Review
Biotechnology & Applied Microbiology
Xiaoshen Hu, Weiyang Zhang, Xiang Li, Dongling Zhong, Yuxi Li, Juan Li, Rongjiang Jin
Summary: Studies have shown that chondrocytes tend to dedifferentiate during cell amplification, losing their physiological properties, but recent research has made progress in redifferentiation control. By modulating various factors in cell culture, similar physiological characteristics and functions to human cartilage tissue have been achieved, offering hope for future cartilage repair and treatment.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Alexandre Dufour, Frederic Mallein-Gerin, Emeline Perrier-Groult
Summary: In this study, a combination of soluble factors, fibrin hydrogel, direct perfusion, and human articular chondrocytes was used to engineer large cartilage tissues. Perfusion preconditioning improved the quality and spatial distribution of the cartilage matrix, resulting in the reconstruction of hyaline cartilage and increased production of the main component of the pericellular matrix by chondrocytes.
APPLIED SCIENCES-BASEL
(2021)
Article
Chemistry, Medicinal
Jiaxiang Gu, Bin Wang, Tianliang Wang, Naichen Zhang, Hongjun Liu, Jianchao Gui, Yiming Lu
Summary: In this study, the effects of cartilage progenitor cells (CPCs), bone marrow mesenchymal stem cells (BMSCs), and chondrocytes (CCs) on cartilage repair were investigated. The results showed that CPCs had higher proliferation ability compared to CCs and BMSCs. Moreover, CPCs seeded in agarose gel exhibited significantly higher levels of collagen, glycosaminoglycan, DNA content, and chondrocyte-related gene expression. Integration strength in the CPCs seeded gel was also higher than the other two gels. These findings suggest that CPCs have advantages over CCs and BMSCs as seed cells in tissue engineering for cartilage repair.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2022)
Article
Orthopedics
Nadine Frerker, Tommy A. Karlsen, Magnus Borstad Lilledahl, Sverre-Henning Brorson, John E. Tibballs, Jan E. Brinchmann
Summary: Scaffold-free engineering with BMP2 and allowing free movement in chondrogenic differentiation medium resulted in firm, elastic cartilage discs rich in type II collagen. This approach may have potential applications in clinical trials.
Article
Biochemistry & Molecular Biology
Xiaoyi Lan, Yan Liang, Esra J. N. Erkut, Melanie Kunze, Aillette Mulet-Sierra, Tianxing Gong, Martin Osswald, Khalid Ansari, Hadi Seikaly, Yaman Boluk, Adetola B. Adesida
Summary: Skin cancer patients with tumorigenic lesions on their noses often require surgical resection resulting in nasal cartilage removal. Autologous tissue-engineered nasal cartilage grafts can help mitigate the problem, despite the issue of inhomogeneous distribution of cartilage matrix. Advances in 3D bioprinting technology offer customizable and anatomically shaped cartilages without the distribution issues.
Article
Biochemistry & Molecular Biology
Maria Oliver-Ferrandiz, Lara Milian, Maria Sancho-Tello, Jose Javier Martin de Llano, Fernando Gisbert Roca, Cristina Martinez-Ramos, Carmen Carda, Manuel Mata
Summary: A novel MACI scaffold composed of a mixed alginate-agarose hydrogel with human dental pulp stem cells (hDPSCs) was proposed, showing superior biomechanical properties and uniform cell distribution. The hDPSCs cultured in the scaffold exhibited good chondrogenic differentiation potential.
Article
Biology
Ning Wang, Yuchen He, Silvia Liu, Meagan J. Makarcyzk, Guanghua Lei, Alexander Chang, Peter G. Alexander, Tingjun Hao, Anne-Marie Padget, Nuria de Pedro, Tsapekos Menelaos, Hang Lin
Summary: The study developed a senescence-relevant OA-like cartilage model using senescent P10-MSCs, displaying both senescent and OA-like phenotypes without using other OA-inducing agents. Gene expression differences observed between P4-MSCs and P10-MSC-derived cartilage tissues were also identified between preserved and damaged OA cartilage regions in human samples. The senescence-initiated OA-like cartilage model was also useful in assessing the efficacy and toxicity of potential DMOADs on both preserved and damaged cartilage in humans.
SCIENCE CHINA-LIFE SCIENCES
(2022)
Article
Oncology
Haneen A. Abusharkh, Olivia M. Reynolds, Juana Mendenhall, Bulent A. Gozen, Edwin Tingstad, Vincent Idone, Nehal Abu-Lail, Bernard J. Van Wie
Summary: The study reveals that gallic acid and mechanical stretching have synergistic effects on promoting the deposition of collagen and glycosaminoglycan in osteoarthritic human articular chondrocytes, as well as selectively upregulating the expression of cartilage-specific proteins while downregulating inflammation markers.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Hannah Agten, Inge Van Hoven, Samuel R. Viseu, Jasper Van Hoorick, Sandra Van Vlierberghe, Frank P. Luyten, Veerle Bloemen
Summary: This study investigates the regenerative potential of human-induced pluripotent stem cell (iPSC)-derived chondrocytes in gelatin methacryloyl (GelMA) hydrogel for hyaline cartilage production. The study demonstrates that GelMA hydrogel supports the chondrocyte phenotype and that encapsulated iPSC-derived chondrocytes can preserve their matrix formation capacity. The study also shows that differentiation medium can promote the formation of hyaline cartilage-like tissue.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Engineering, Biomedical
A. R. Chin, J. M. Taboas, A. J. Almarza
Summary: Research showed that mandibular condyle cartilage cells do have the potential for cartilage regeneration, but they are more sensitive to hydrogel materials compared to costal cartilage cells.
ANNALS OF BIOMEDICAL ENGINEERING
(2021)
Article
Polymer Science
Liang Lu, Xifu Shang, Bin Liu, Weijian Chen, Yu Zhang, Shuyun Liu, Xiang Sui, Aiyuan Wang, Quanyi Guo
Summary: This paper explores the application value of tissue engineering in vitro for the construction of artificial cartilage. By seeding healthy porcine chondrocytes on articular cartilage extracellular matrix (ACECM) scaffolds, it was found that the cells performed well in terms of adhesion and growth on the scaffolds, and they secreted collagen type II. Moreover, the porcine ACECM scaffolds showed good biocompatibility and could be used for xenotransplantation without significant immune inflammatory response. Therefore, ACECM-oriented scaffold is an ideal natural biomaterial for cartilage tissue engineering.
Article
Orthopedics
Meike W. A. Kleuskens, Joao F. Crispim, Marina van Doeselaar, Corrinus C. van Donkelaar, Rob P. A. Janssen, Keita Ito
Summary: In this study, a novel chondrocyte suspension expansion protocol was used to self-assemble human chondrocytes into cartilage organoids using porcine notochordal cell-derived matrix. The resulting organoids showed similar histologic appearance and gene expression profile, indicating their potential for cartilage regeneration and as an in vitro model for studying pathways or drug development.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Nadine Frerker, Tommy A. A. Karlsen, Maria Stensland, Tuula A. A. Nyman, Simon Rayner, Jan E. E. Brinchmann
Summary: Focal lesions of articular cartilage can cause pain and reduced joint function, leading to osteoarthritis if untreated. In vitro generated, scaffold-free autologous cartilage discs made from articular chondrocytes were compared to those made from bone marrow-derived mesenchymal stromal cells. Articular chondrocytes produced more extracellular matrix and contained more articular cartilage proteins, while mesenchymal stromal cells had more proteins associated with cartilage hypertrophy and bone formation. MicroRNA analysis suggested that differential expression of microRNAs played a key role in the differential synthesis of proteins. Therefore, articular chondrocytes are preferred for engineering articular cartilage.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Engineering, Biomedical
Xiaoyi Lan, Zhiyao Ma, Melanie Kunze, Aillette Mulet-Sierra, Martin Osswald, Khalid Ansari, Hadi Seikaly, Yaman Boluk, Adetola B. Adesida
Summary: Hydrogels with different crosslinking densities were used to investigate their effects on chondrocyte phenotype and cellular interaction. The results showed that the storage modulus of the hydrogel did not significantly alter the cartilaginous gene expression of chondrocytes. However, the gene expression of PPAR-gamma was positively correlated with crosslinking density. RNA-seq analysis revealed numerous genes that were significantly correlated to crosslinking density, which warrants further investigation.
ANNALS OF BIOMEDICAL ENGINEERING
(2023)
Review
Biotechnology & Applied Microbiology
Marcin Szustak, Edyta Gendaszewska-Darmach
Summary: Nanocellulose is a promising biocompatible biomaterial with good mechanical properties for use as a scaffold in cartilage tissue engineering. The limited regenerative capacity of cartilage due to its lack of vascularization and sparsely distributed chondrocytes poses challenges for cell infiltration, making nanocellulose scaffolds a potential solution. The scaffolds have been shown to induce stem cell differentiation into chondrocyte phenotypes, offering opportunities for cartilage tissue development.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
