Article
Biochemistry & Molecular Biology
Xianchen Liu, Yukari Shiwaku, Ryo Hamai, Kaori Tsuchiya, Tetsu Takahashi, Osamu Suzuki
Summary: This study examined the effect of synthetic octacalcium phosphate (OCP) on tendon stem/progenitor cells (TSPCs) and found that OCP promoted the osteogenic differentiation of TSPCs. The difference in differentiation was related to the ionic environment induced by OCP and calcium-deficient hydroxyapatite (CDHA), which may be associated with the hydrolysis of OCP into CDHA. These findings support previous in vivo observations of OCP's healing function in rabbit rotator cuff tendon.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Laura Alves, Vanessa Machado, Joao Botelho, Jose Joao Mendes, Joaquim M. S. Cabral, Claudia L. da Silva, Marta S. Carvalho
Summary: Cell-based therapies using PDLSC may be a promising alternative to MSC(M) and MSC(AT) for periodontal regeneration, as PDLSC showed enhanced proliferative and osteogenic potential. PDLSC expressed osteogenic markers and had higher calcium content and upregulated osteogenic/periodontal genes compared to MSC(M) and MSC(AT). CD146 was specifically expressed by PDLSC, which also had a higher proliferative capacity. However, alkaline phosphatase activity did not increase in PDLSC.
Article
Cell Biology
Chih-Yi Yang, Rita Jui-Hsien Lu, Ming-Kang Lee, Felix Shih-Hsian Hsiao, Ya-Ping Yen, Chun-Chun Cheng, Pu-Sheng Hsu, Yi-Tzang Tsai, Shih-Kuo Chen, I-Hsuan Liu, Pao-Yang Chen, Shau-Ping Lin
Summary: MSCs lacking DNMT3L exhibit reduced colony forming ability and impaired osteogenesis in vitro, with differentially regulated genes associated with bone morphology-related phenotypes. The observed defects are hypothesized to result from pre-deposited epigenetic signatures from DNMT3L-expressing progenitors, rather than a direct effect of missing DNMT3L in MSCs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Engineering, Biomedical
Linghao Xiao, Yukari Shiwaku, Ryo Hamai, Kazuyoshi Baba, Kaori Tsuchiya, Satoshi Imazato, Keiichi Sasaki, Osamu Suzuki
Summary: Previous research has shown that octacalcium phosphate (OCP) enhances macrophage accumulation and modifies the initial inflammatory response. This study investigates the behavior of macrophages and their role in bone regeneration during the early inflammatory stage of OCP-mediated osteogenesis. The findings suggest that OCP not only regulates immune function through macrophage polarization and secretion profile, but also positively impacts osteogenesis.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2023)
Article
Multidisciplinary Sciences
Cheng Su, Xiao Zheng, Yanjin He, Li Long, Wenchuan Chen
Summary: This study identified aberrant expression of circRNAs in hUCMSCs during osteogenic differentiation, constructed competing endogenous RNA networks using bioinformatics tools, elucidated underlying mechanisms, and validated the osteogenic-promoting effect of a specific circRNA.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Shuang Zhang, Jeroen van de Peppel, Marijke Koedam, Johannes P. T. M. van Leeuwen, Bram C. J. van der Eerden
Summary: In this study, TNS3 was found to be involved in the fate decision of human bone marrow-derived stromal cells (BMSCs), regulating osteogenesis and adipogenesis through the control of RhoA activity and cytoskeletal reorganization. The findings highlight the crucial role of TNS3 in bone metabolism.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Zhenghai Zhang, Lulu Gong, Min Li, Guoshuai Wei, Yan Liu
Summary: This study investigated the effects of NF and FFF scaffolds on the behavior of hBMSCs using MFCS, IFS, and SVM, revealing that NF scaffolds induce the expression of more osteogenic-related genes in hBMSCs. Enrichment analysis of common genes suggests that NF scaffolds may enhance the osteogenic differentiation ability of hBMSCs.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2021)
Article
Pharmacology & Pharmacy
Victor Gustavo Balera Brito, Mariana Sousa Patrocinio, Ayna Emanuelli Alves Barreto, Sabrina Cruz Tfaile Frasnelli, Vanessa Soares Lara, Carlos Ferreira Santos, Sandra Helena Penha Oliveira
Summary: The study demonstrated that Telmisartan (TELM) inhibited osteogenic differentiation and mineralization of SHR BMSC, favoring an adipogenic phenotype due to Pparg upregulation.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Jiayu Lu, Jiayue Sun, Derong Zou, Jinlin Song, Sheng Yang
Summary: Graphene coating on titanium implants can enhance osteogenic activity by improving protein adsorption and cell adhesion, as well as sustained release of growth factors, providing a feasible alternative for improving bone formation in dental implants.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Cell Biology
Jiachen Lin, Zhifa Zheng, Jieying Liu, Guihua Yang, Ling Leng, Hai Wang, Guixing Qiu, Zhihong Wu
Summary: Nutritional microenvironment plays a crucial role in determining the specification of progenitor cells, with lipid availability impacting osteogenesis in skeletal progenitors. The study found that lipid scarcity suppresses the osteogenic differentiation of BMSCs, while LRP5 mediates lipid uptake in BMSCs and influences osteogenic specification.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell & Tissue Engineering
Atsumu Kouketsu, Keiko Matsui, Tadashi Kawai, Yushi Ezoe, Tetsu Takahashi, Shinji Kamakura
Summary: Our study revealed that the composite of OCPcolTPTD enhances bone regeneration more evenly and homogenously than OCPcol, suggesting its potential as a novel clinical technique for bone regeneration or repair.
TISSUE ENGINEERING PART A
(2022)
Article
Polymer Science
Krishnamurithy Genasan, Mohammad Mehrali, Tarini Veerappan, Sepehr Talebian, Murali Malliga Raman, Simmrat Singh, Sasikumar Swamiappan, Mehdi Mehrali, Tunku Kamarul, Hanumantha Rao Balaji Raghavendran
Summary: The study investigated the physicochemical, biocompatible, and osteoinductive properties of gellan-chitosan incorporated with different percentages of calcium silicate in human bone-marrow mesenchymal stromal cells (hBMSCs). Results showed that the 40% calcium silicate incorporation exhibited the most promising osteogenic potential in hBMSCs, making it a potential biomaterial for treating critical bone defects.
Article
Immunology
Chiara Tomasoni, Corinne Arsuffi, Samantha Donsante, Alessandro Corsi, Mara Riminucci, Andrea Biondi, Alice Pievani, Marta Serafini
Summary: Acute myeloid leukemia (AML) cells have a negative impact on the osteogenic differentiation of normal bone marrow stromal cells (BMSCs), and direct interaction between AML cells and BMSCs plays a vital role in this process. AML-mediated activation of Notch signaling is involved in the ineffective osteogenic commitment of BMSCs, resulting in the formation of a pre-osteoblast-rich niche supportive of leukemia development in the bone marrow.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Tomislav Smoljo, Barbara Tomic, Hrvoje Lalic, Vilma Dembitz, Josip Batinic, Antonio Bedalov, Dora Visnjic
Summary: This study demonstrates that bone marrow stroma reduces differentiation of acute myeloid leukemia (AML) induced by low-dose cytarabine (LDAC). These findings provide insights into the limited occurrence of terminal differentiation observed in AML patients and suggest a potential explanation for this observation.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Oncology
Rafaela Rossetti, Felipe Augusto Ros, Lucas Eduardo Botelho de Souza, Juliana de Matos Maconetto, Pericles Natan Mendes da Costa, Fernanda Ursoli Ferreira, Josiane Serrano Borges, Julianne Vargas de Carvalho, Nayara Patricia Morotti, Simone Kashima, Dimas Tadeu Covas
Summary: The bone marrow microenvironment may contain two distinct populations of mesenchymal stromal cells (MSC), one located perivascularly and the other in the endosteum. Recent research has focused on better understanding these MSC subsets in order to improve their clinical applications. Studies indicate that compact bone is a rich source of MSC and offers lower risk of contamination by hematopoietic cells. Compact bone-derived MSC also show higher osteogenic potential compared to bone marrow-derived MSC. These findings suggest the presence of functionally different MSC populations in bone marrow and compact bone.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Engineering, Biomedical
Kei-ichiro Miura, Yoshinori Sumita, Fumihiko Kajii, Hidenori Tanaka, Shinji Kamakura, Izumi Asahina
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2020)
Article
Cell & Tissue Engineering
Atsumu Kouketsu, Keiko Matsui, Tadashi Kawai, Yushi Ezoe, Toshiki Yanagisawa, Ayato Yasuda, Tetsu Takahashi, Shinji Kamakura
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2020)
Article
Chemistry, Physical
Jianxin Zhao, Yoshitomo Honda, Tomonari Tanaka, Yoshiya Hashimoto, Naoyuki Matsumoto
Article
Engineering, Biomedical
Toshiki Yanagisawa, Ayato Yasuda, Ria I. Makkonen, Shinji Kamakura
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2020)
Article
Biochemistry & Molecular Biology
Yoshitomo Honda, Anqi Huang, Tomonari Tanaka, Xiaoyu Han, Beiyuan Gao, Haitao Liu, Xinchen Wang, Jianxin Zhao, Yoshiya Hashimoto, Kazuyo Yamamoto, Naoyuki Matsumoto, Shunsuke Baba, Makoto Umeda
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Chemistry, Physical
Xiaoyu Han, Yoshitomo Honda, Tomonari Tanaka, Kazuki Imura, Yoshiya Hashimoto, Kazushi Yoshikawa, Kazuyo Yamamoto
Article
Biochemistry & Molecular Biology
Jinzhao Lyu, Yoshiya Hashimoto, Yoshitomo Honda, Naoyuki Matsumoto
Summary: Scaffolds play a crucial role in bone defect repair by stimulating cell proliferation and differentiation. The study found that using CellSaic containing rDPSCs showed better outcomes for promoting bone regeneration compared to using rBMSCs, indicating DPSCs as a promising source for bone tissue regenerative therapy, particularly in craniofacial bone defect repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Xinchen Wang, Yoshitomo Honda, Jianxin Zhao, Hidetoshi Morikuni, Aki Nishiura, Yoshiya Hashimoto, Naoyuki Matsumoto
Summary: The study demonstrates that beta-TCP granules can induce senescent-like cells, and the administration of senolytics may improve bone-forming ability by inhibiting senescence-associated mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Yanan Gong, Yoshitomo Honda, Tetsuya Adachi, Elia Marin, Kazushi Yoshikawa, Giuseppe Pezzotti, Kazuyo Yamamoto
Summary: The study investigated the effect of different types of Si3N4 granules on rat dental pulp cells and found that sintered Si3N4 granules significantly promoted cell attachment, upregulated odontogenic marker genes, and enhanced mineralization nodule formation. The sintering process positively altered the surface properties of Si3N4 granules and facilitated odontogenic differentiation, suggesting potential clinical applications in dentistry.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Polymer Science
Beiyuan Gao, Yoshitomo Honda, Yoichi Yamada, Tomonari Tanaka, Yoshihiro Takeda, Takayuki Nambu, Shunsuke Baba
Summary: The newly developed material vhEc-GS-beta showed high stability and bone-forming ability in experiments, achieved through thermal cross-linking induced in gelatin sponges by vacuum heating treatment. This insight could potentially lead to the development of novel materials with good operability and bone-forming ability in bone regenerative medicine.
Article
Chemistry, Multidisciplinary
Nobuhito Tsumano, Hirohito Kubo, Rie Imataki, Yoshitomo Honda, Yoshiya Hashimoto, Masahiro Nakajima
Summary: The study demonstrated that a composite gelatin sponge of alpha-tricalcium phosphate mixed with gelatin scaffolds induced bone regeneration in a rat calvarial defect model, with the group containing DFAT cells showing better results.
APPLIED SCIENCES-BASEL
(2021)
Article
Chemistry, Physical
Kosuke Nakano, Hirohito Kubo, Masahiro Nakajima, Yoshitomo Honda, Yoshiya Hashimoto
Article
Engineering, Biomedical
Nguyen Dien Bien, Kei-ichiro Miura, Yoshinori Sumita, Yuya Nakatani, Rena Shido, Fumihiko Kajii, Shinji Kamakura, Izumi Asahina
JOURNAL OF HARD TISSUE BIOLOGY
(2020)
Article
Dentistry, Oral Surgery & Medicine
Toshiki Yanagisawa, Ayato Yasuda, Ria Makkonen, Shinji Kamakura
CLINICAL AND EXPERIMENTAL DENTAL RESEARCH
(2020)
Article
Multidisciplinary Sciences
Takashi Sasaki, Kuniyasu Niizuma, Atsushi Kanoke, Keiko Matsui, Shogo Ogita, Sherif Rashad, Tadashi Kawai, Mika Watanabe, Hidenori Endo, Tetsu Takahashi, Shinji Kamakura, Teiji Tominaga