期刊
TISSUE ENGINEERING PART A
卷 14, 期 1, 页码 195-206出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/ten.a.2006.0370
关键词
-
资金
- NATIONAL CENTER FOR RESEARCH RESOURCES [P40RR017447] Funding Source: NIH RePORTER
- NCRR NIH HHS [P40RR017447] Funding Source: Medline
In this study, the fabrication and characterization of highly porous composites composed of poly (epsilon-caprolactone) and bioactive mesoporous silicon (BioSilicon) prepared using salt-leaching and microemulsion/freeze-drying methods are described. The role of silicon, along with porosity, in the scaffolds on calcium phosphate deposition was assessed using acellular in vitro calcification analyses. The presence of bioactive silicon in these scaffolds is essential for the deposition of calcium phosphate while the samples are immersed in simulated body fluid (SBF). Silicon-containing scaffolds produced using salt-leaching methods are more likely to calcify as a consequence of SBF exposure than those produced using microemulsion methods. In vitro proliferation and cell viability assays of these porous composites using human embryonic kidney fibroblast cells indicate that no cytotoxic effects are present in the scaffolds under the conditions used. Preliminary analyses of bone sialoprotein and alkaline phosphatase expression using orthopedically relevant mesenchymal cells derived from bone marrow suggest that such scaffolds are capable of mediating osteoblast differentiation. Overall, the results show that these porous silicon-containing polymer scaffolds enhance calcification, can be considered nontoxic to cells, and support the proliferation, viability, attachment, and differentiation of bone precursor cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据