期刊
TISSUE & CELL
卷 44, 期 2, 页码 95-100出版社
CHURCHILL LIVINGSTONE
DOI: 10.1016/j.tice.2011.12.001
关键词
Estrogen receptor alpha; Oocytes; Follicle cells; Immunohistochemistry; Neomysis japonica
资金
- National Nature Science Foundation of China [30700609, 30871927]
- innovation research group developing project in the universities of Shanghai (nutrition, feed and environment of animal aquaculture)
- Shanghai Leading Academic Discipline Project
- Shanghai modern agricultural industry technology system for Chinese mitten crab [D2010100208]
- Shanghai Postgraduate Education Innovation Program [B94001000062]
Estrogen induces oocytes development and vitellogenesis in crustacean by interacting with estrogen receptor (ER) subtypes. In the present study, we detect for the first time the ER alpha in oocytes and follicle cells and hepatopancreas cells of mysis by immunohistochemistry using a specific ER alpha antibody. ER alpha was mainly localized in the nuclei of oocytes and follicle cells, while mainly detected in nuclei of oogonia (OG), previtellogenic oocyte (PR) and endogenous vitellogenic oocyte (EN) at previtellogenic and early vitellogenic stage (I-early III). Follicle cells in all stages of ovary (all vitellogenic stages) showed strong ER alpha positive reaction, and they were able to gradually move to oocytes during the development of oocytes. In addition, ER alpha was also localized in the nuclei and cytoplasm of four hepatopancreas cells (including E-, R-, F- and B-cell) in all ovary stages. These findings suggest, for the first time to our knowledge, that there could be a close link between oogenesis, follicle cells, hepatopancreas cells and endocrine regulation, and estrogens might be involved in the regulation of oocytes at early ovarian stage in mysis. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据