Article
Hematology
Georges Jourdi, Sofia Ramstrom, Ruchika Sharma, Tamam Bakchoul, FC-PFT TP Study Grp
Summary: The aim of this study was to provide expert consensus guidance on the use of flow cytometry for the evaluation of platelet function in patients with low platelet counts. Through literature review and focus group discussions, consensus recommendations were made on sample collection, preanalytical variables, sample type, and results expression. These recommendations would allow standardization of protocols in future clinical studies.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Hematology
Christine S. M. Lee, Maria Selvadurai, Leonardo Pasalic, James Yeung, Maria Konda, Geoffrey W. Kershaw, Emmanuel J. Favaloro, Vivien M. Chen
Summary: This study found that heparin-induced thrombocytopenia (HIT), together with platelet agonists, increased the proportion of procoagulant platelets, which may contribute to thrombotic risk. This discovery could lead to a new treatment strategy, and the procoagulant platelet assay may serve as a rapid, clinically relevant, and accurate functional test for detecting pathological HIT antibodies.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Immunology
Rie Tabata, Chiharu Tabata
Summary: Some patients with immune thrombocytopenia show good clinical courses, while others may experience severe bleeding complications. The presence of CD10+/HLA-DR+ cells and their percentage in bone marrow may be correlated with the response to steroid therapy and the risk of serious bleeding complications in immune thrombocytopenia patients. Monitoring the percentage of CD10+/HLA-DR+ cells could be helpful in identifying patients at high risk of severe bleeding complications.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Hematology
Alessandro Aliotta, Debora Bertaggia Calderara, Maxime G. Zermatten, Lorenzo Alberio
Summary: The study demonstrated contrasting patterns in the intracellular ion concentrations of COAT-activated platelets compared to non-COAT platelets, with the former showing high and prolonged calcium concentration, transient sodium increase, and fast potassium efflux. Inhibition of the sodium-calcium exchanger (NCX) reduced calcium mobilization and prevented the formation of procoagulant platelets, highlighting the critical and dichotomous role of NCX during platelet activation induced by convulxin-plus-thrombin.
THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Hematology
Predrag Miljic, Abdelrahman Noureldin, Michelle Lavin, Sajida Kazi, Analia Sanchez-Luceros, Paula D. James, Maha Othman
Summary: This study examined the diagnostic and management approaches and outcomes in women with type 2B von Willebrand disease during pregnancy. The findings highlight the challenges and uncertainties in managing these patients, such as bleeding complications, unknown target safe VWF level, diverse hemostatic management practices, and varied views on delivery mode and anesthesia use.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Veterinary Sciences
Anja Kuhn, Kristina Weiler, Graham Gibbs, Gottfried Prechtl, Natali Bauer, Andreas Moritz
Summary: This study aimed to evaluate the consistency of using a moving threshold method to measure canine r-PLTs with flow cytometry, and to determine the corresponding reference intervals.
VETERINARY CLINICAL PATHOLOGY
(2023)
Article
Cell Biology
Marie-Astrid van Dievoet, Davide Brusa, Marie Octave, Sandrine Horman, Xavier Stephenne
Summary: This article describes an activation problem encountered with anti-GPVI, clone HY101, in a whole blood multicolor flow cytometry panel. Platelet activation was observed after the addition of anti-GPVI, as indicated by increased expression of activation markers CD62P, PAC-1, CD63, and CD107a. This finding was further confirmed by platelet aggregation studies.
Editorial Material
Hematology
Giovanni Riva, Mario Luppi, Enrico Tagliafico
Summary: The study shows that AI-based methods can improve flow cytometry diagnostics in haematology. By exploring an emerging machine learning approach, the research demonstrates that AI-driven computational analysis may be a robust and feasible tool for advanced diagnostics of haematological malignancies.
BRITISH JOURNAL OF HAEMATOLOGY
(2023)
Article
Hematology
Matthew S. Hindle, Benjamin E. J. Spurgeon, Lih T. Cheah, Beth A. Webb, Khalid M. Naseem
Summary: The study identified novel platelet subpopulations that suggest differential regulation of platelet functions by PGI(2) using multicolor flow cytometry assays and computational analysis.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Microbiology
Firas S. Midani, Lawrence A. David
Summary: Cross feeding between microbes is widespread but not fully understood. In this study, a multicolor flow cytometry combined with a machine learning-based classifier was used to rapidly quantify species abundances in simple microbial communities. The results showed evidence of cooperation and competition within the community, as well as the impact of different sugars on microbial productivity and community diversity. This approach provides an economic and tractable model system for studying microbial ecology and can be extended to more species and complex environments.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Hematology
Guillaume Bonnard, Antoine Babuty, Romain Collot, Domitille Costes, Nicolas Drillaud, Marion Eveillard, Antoine Neel, Alexandra Espitia, Agathe Masseau, Anais Wahbi, Mohamed Hamidou, Marie C. Bene, Marc Fouassier
Summary: This study found that platelet sialylation and activation levels, as well as immature platelet counts, could help differentiate between ITP and IT, providing potential useful diagnostic tools for physicians in complex cases of thrombocytopenia.
ANNALS OF HEMATOLOGY
(2021)
Article
Hematology
Maria N. Barrachina, Lidia Hermida-Nogueira, Luis A. Moran, Vanessa Casas, Sarah M. Hicks, Aurelio M. Sueiro, Ying Di, Robert K. Andrews, Steve P. Watson, Elizabeth E. Gardiner, Joaquin Abian, Montserrat Carrascal, Maria Pardo, Angel Garcia
Summary: In this study, a comparative phosphoproteomic analysis of platelets from obese patients revealed up-regulated levels of phosphorylation events and altered signaling pathways compared to lean controls. Flow cytometry assays and mass spectrometry validation confirmed platelet dysfunction in obese individuals, including higher surface levels of GPVI and CLEC2. Soluble GPVI levels were also found to be elevated in plasma from obese patients, suggesting increased GPVI signaling activation.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Anne Runser, Caroline Schaning, Frederic Allemand, Jean Amiral
Summary: HIT is a thrombocytopenia caused by heparin with a paradoxical high thrombotic risk, and its diagnosis involves clinical scoring and laboratory testing, with antigen binding assays being the first-line routine tests and functional assays being necessary to exclude false-positive results. Flow cytometry is a possible alternative to detect platelet activation, but lacks standardization.
Article
Biochemical Research Methods
Staffan Holmberg-Thyden, Kirsten Gronbaek, Anne Ortved Gang, Daniel El Fassi, Sine Reker Hadrup
Summary: Multicolor flow cytometry is crucial for studying the immune system, but variations in instrument performance can lead to common analytical errors. By systematically pairing colors with markers and optimizing for specific equipment, designing comprehensive panels for flow cytometry experiments can help overcome these limitations. Unsupervised clustering techniques are then used to identify important subpopulations that may not be detected through traditional gating methods, allowing for better handling of the large amounts of data generated.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Hematology
Justine Brodard, Vincent Benites, Denise Stalder Zeerleder, Michael Nagler
Summary: The study investigated a rapid flow cytometer-based assay for the diagnostic accuracy of HIT, showing high specificity but limited sensitivity in verifying HIT patients. The results suggest that other rapid functional assays should be studied in appropriately designed diagnostic accuracy studies.
THROMBOSIS RESEARCH
(2021)
Article
Hematology
Karina Meijer, Waander van Heerde, Keith Gomez
Summary: Rare bleeding disorders have significant morbidity but are often underdiagnosed globally. Advances in genomic testing and specialist laboratory assays have greatly improved the diagnostic capabilities, leading to the discovery of new genetic causes for rare diseases and a better understanding of their underlying molecular pathology.
Letter
Hematology
Lars L. F. G. Valke, Erik A. M. Beckers, Nicole M. A. Blijlevens, Waander L. van Heerde, Saskia E. M. Schols
Article
Hematology
Lars L. F. G. Valke, Laura H. Bukkems, Wideke Barteling, Britta A. P. Laros-van Gorkom, Nicole M. A. Blijlevens, Ron A. A. Mathot, Waander L. van Heerde, Saskia E. M. Schols
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2020)
Article
Hematology
Joline L. Saes, Marieke J. A. Verhagen, Karina Meijer, Marjon H. Cnossen, Roger E. G. Schutgens, Marjolein Peters, Laurens Nieuwenhuizen, Felix J. M. van der Meer, Ilmar C. Kruis, Waander L. van Heerde, Saskia E. M. Schols
Article
Pharmacology & Pharmacy
Laura H. Bukkems, Lars L. F. G. Valke, Wideke Barteling, Britta A. P. Laros-van Gorkom, Nicole M. A. Blijlevens, Marjon H. Cnossen, Waander L. van Heerde, Saskia E. M. Schols, Ron A. A. Mathot
Summary: The study aimed to develop a population PK/PD model based on FVIII activity levels and NHA patterns, in order to assess the relationship between individual hemostatic capacity and dosing methods in hemophilia A patients.
BRITISH JOURNAL OF CLINICAL PHARMACOLOGY
(2022)
Article
Hematology
Dominique P. M. S. M. Maas, Joline L. Saes, Nicole M. A. Blijlevens, Marjon H. Cnossen, Paul L. den Exter, Ilmar C. Kruis, Karina Meijer, Laurens Nieuwenhuizen, Marjolein Peters, Roger E. G. Schutgens, Waander L. van Heerde, Saskia E. M. Schols
Summary: Patients with rare inherited bleeding disorders (RBDs) exhibit heterogeneity in treatment plans and frequently experience bleeding after invasive procedures. Adequate peri-procedural therapy can reduce bleeding risk. Improved guidelines should include specific treatment recommendations for each type of RBD and emphasize the relevance of individual bleeding history.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Hematology
Dominique P. M. S. M. Maas, Ferdows Atiq, Nicole M. A. Blijlevens, Paul P. T. Brons, Sandy Krouwel, Britta A. P. Laros-van Gorkom, Frank W. G. Leebeek, Laurens Nieuwenhuizen, Selene C. M. Schoormans, Annet Simons, Danielle Meijer, Waander L. van Heerde, Saskia E. M. Schols
Summary: This study reveals the clinical and laboratory characteristics of genetically confirmed VWD type 2M patients, showing that they have a relatively mild clinical phenotype, except for bleeding after surgery and delivery. Laboratory phenotype varies and depends on the underlying genetic variant. The addition of genotyping to the current phenotypic characterization may improve diagnosis and classification of VWD.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Biochemistry & Molecular Biology
Daan Sondag, Jona Merx, Emiel Rossing, Thomas J. Boltje, Dennis W. P. M. Lowik, Frank H. T. Nelissen, Mark van Geffen, Cornelis van 't Veer, Waander L. van Heerde, Floris P. J. T. Rutjes
Summary: This article introduces a luminescent assay using a peptide-based probe to screen and validate M-Pro inhibitors. The method has been proven effective in screening potential inhibitors.
Article
Hematology
Karina Meijer, Waander van Heerde, Keith Gomez
Summary: Advancements in genomic testing and specialist laboratory assays have greatly improved the diagnostic accuracy of rare bleeding disorders, leading to the discovery of new genetic causes and a better understanding of the underlying molecular pathology.
Letter
Hematology
Dominique P. M. S. M. Maas, Waander L. van Heerde, Saskia E. M. Schols
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Hematology
Ferdows Atiq, Jessica Heijdra, Fleur Snijders, Johan Boender, Eva Kempers, Waander L. van Heerde, Dominique P. M. S. M. Maas, Sandy Krouwel, Selene C. Schoormans, Joke de Meris, Saskia E. M. Schols, Karin P. M. van Galen, Johanna G. van der Bom, Marjon H. Cnossen, Karina Meijer, Karin Fijnvandraat, Jeroe Eikbenboom, Frank W. G. Leebeek
Summary: Patients with type 1 von Willebrand disease who have a VWF gene variant show a weaker response to desmopressin, compared to those without a variant. On the other hand, patients without a VWF gene variant have a complete response to desmopressin. Desmopressin response in patients with the same variant is comparable.
Article
Hematology
Ferdows Atiq, Johan Boender, Waander L. van Heerde, Juan M. Tellez Garcia, Selene C. Schoormans, Sandy Krouwel, Marjon H. Cnossen, Britta A. P. Laros-van Gorkom, Joke de Meris, Karin Fijnvandraat, Johanna G. van der Bom, Karina Meijer, Karin P. M. van Galen, Jeroen Eikenboom, Frank W. G. Leebeek
Summary: Genotyping plays an important role in understanding the pathogenic mechanisms and phenotypic variability of von Willebrand disease (VWD). By analyzing the VWF gene in 390 VWD patients, various gene variants associated with VWD were identified, and the mechanisms of VWF level reduction were determined. Additionally, significant differences in clinical features were observed between type 1 VWD patients with and without VWF gene variants, highlighting the importance of genotyping in clinical management.
Article
Hematology
Lars L. F. G. Valke, Danielle Meijer, Laurens Nieuwenhuizen, Britta A. P. Laros-van Gorkom, Nicole M. A. Blijlevens, Waander L. van Heerde, Saskia E. M. Schols
Summary: Analysis of fibrinolytic disorders in selected patients has a high diagnostic yield. Incorporating fibrinolytic analysis in the diagnostic workup of patients with bleeding of unknown cause can improve diagnosis and management of their bleeding episodes.
RESEARCH AND PRACTICE IN THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Medicine, General & Internal
F. Atiq, J. L. Saes, M. C. Punt, K. P. M. van Galen, R. E. G. Schutgens, K. Meijer, M. H. Cnossen, B. A. P. Laros-Van Gorkom, M. Peters, L. Nieuwenhuizen, M. J. H. A. Kruip, J. de Meris, J. G. van der Bom, F. J. M. van der Meer, K. Fijnvandraat, I. C. Kruis, W. L. van Heerde, H. C. J. Eikenboom, Frank W. G. Leebeek, S. E. M. Schols
Summary: In autosomal inherited bleeding disorders, women are more likely to be referred for bleeding, have a longer diagnostic delay, and often require treatment for sex-specific bleeding.
Article
Hematology
Britta Laros-van Gorkom, Pal Andre Holme, Christine Joch, Tobias Rogosch, Annette Feussner, William McKeand, John Roberts, Waander van Heerde
Article
Hematology
E. O. Artemenko, S. I. Obydennyi, K. S. Troyanova, G. A. Novichkova, D. Y. Nechipurenko, M. A. Panteleev
Summary: In this study, we investigated the adhesive properties of circulating plasma-derived and platelet-derived microvesicles (MVs) and their potential role in thrombus growth. The results showed that both types of MVs weakly bound fibrinogen but did not stably adhere to immobilized fibrinogen. Moreover, the presence of these MVs did not significantly affect thrombus formation. Therefore, MVs present in the plasma of healthy individuals are unlikely to directly contribute to thrombus formation under arterial flow conditions.
THROMBOSIS RESEARCH
(2024)
Letter
Hematology
Giuseppe Lippi, Camilla Mattiuzzi, Emmanuel J. Favaloro
THROMBOSIS RESEARCH
(2024)
Article
Hematology
Marium Malik, Maha Al-Ghafry, Abraham Haimed, Julia Su, Maribel Lema, Linda Shore-Lessersson, Suchitra S. Acharya
Summary: This study analyzed and evaluated leukemia-associated coagulopathy in newly diagnosed pediatric leukemia patients using CCTs and ROTEM. The results showed that fibrinogen and platelets played a significant role in causing coagulopathies in this population. This suggests the utility of using CCTs and ROTEM in the pediatric population to evaluate hemostatic function and guide blood product replacement.
THROMBOSIS RESEARCH
(2024)
Review
Hematology
Mohammad M. Elahi, Alexandra N. Witt, Edward L. G. Pryzdial, Paul B. McBeth
Summary: Thrombotic disease may be underdiagnosed in microgravity conditions, and the underlying factors are still poorly understood. A case of internal jugular vein thrombosis was recently diagnosed in a low-risk female astronaut, and six out of ten additional crew members showed risk factors for jugular venous flow. Observations in space and studies on Earth suggest that microgravity affects blood flow, coagulation, and vascular function.
THROMBOSIS RESEARCH
(2024)
Review
Hematology
Stian Ingemann-Molden, Christina Krogner Caspersen, Nanna Rolving, Anette Arbjerg Hojen, Frederikus A. Klok, Erik L. Grove, Barbara Cristina Brocki, Jane Andreasen
Summary: This study found that no single PROM covered all aspects considered important by PE patients. PROMs recommended in the ICHOM core set covered most aspects, but worrisome thoughts, hypervigilance around symptoms, and uncertainty of illness were not covered.
THROMBOSIS RESEARCH
(2024)
Article
Hematology
Haomin Yan, Tsutomu Sasaki, Yasufumi Gon, Kumiko Nishiyama, Hideaki Kanki, Hideki Mochizuki
Summary: The incidence of cancer-associated stroke has increased with the prolonged survival times of cancer patients. In this study, the researchers focused on the driver gene KRAS and found that KRAS mutations exacerbated neurological deficits after stroke and promoted tumor growth. They also identified the upregulation of pro-inflammatory responses and the promotion of STAT3 phosphorylation in mice with KRAS mutations. Additionally, the researchers found that colorectal cancer patients with stroke onset within 3 months after cancer diagnosis had a worse prognosis.
THROMBOSIS RESEARCH
(2024)
Letter
Hematology
Tomoki Togashi, Satomi Nagaya, Makiko Meguro-Horike, Haruto Matsumoto, Yuta Imai, Koichi Yamaguchi, Yoshinari Fujii, Haruka Moriya, Yuika Kikuchi, Ibuki Yasuda, Shin-ichi Horike, Eriko Morishita
THROMBOSIS RESEARCH
(2024)
Article
Hematology
Marta Najarro, Winnifer Briceno, Carmen Rodriguez, Alfonso Muriel, Sara Gonzalez, Ana Castillo, Ignacio Jara, Parth Rali, Catalin Toma, Behnood Bikdeli, David Jimenez
Summary: The CPES score is a useful tool in identifying the risk of hemodynamic deterioration in normotensive patients with acute PE, with acceptable C-statistic, excellent specificity, and low positive predictive value.
THROMBOSIS RESEARCH
(2024)
Article
Hematology
Lei Liu, Huifang Xu, Jian Wang, Haiyan Wang, Saisai Ren, Qian Huang, Mingyan Zhang, Hui Zhou, Chunyan Yang, Lu Jia, Yu Huang, Hao Zhang, Yanling Tao, Ying Li, Yanan Min
Summary: This study investigated the gut microbiota metabolites and cytokines, chemokines, and growth factors in patients with ITP and found their association with treatment responsiveness. The findings suggest that imbalanced secretion of cyto(chemo)kins/growth factors and inadequate levels of TMAO are characteristic of patients with ITP.
THROMBOSIS RESEARCH
(2024)
Article
Hematology
Roxanne Mcknight, Mohammad Salameh, Lee Ann Jones, Greta Anton, Lindsay Carter
Summary: This study evaluated the safety of fixed-dose heparin titration boluses in underweight patients with VTE. The results showed that although underweight patients required more frequent holding of heparin, there was no significant increase in bleeding risk.
THROMBOSIS RESEARCH
(2024)
