Article
Biochemistry & Molecular Biology
Nory Marino-Ocampo, Diego F. Rodriguez, Daniel Guerra Diaz, Daniel Zuniga-Nunez, Yorley Duarte, Denis Fuentealba, Flavia C. Zacconi
Summary: Direct FXa inhibitors, including rivaroxaban, apixaban, edoxaban, and betrixaban, are widely used for thromboprophylaxis in cardiovascular diseases. This research focuses on the interaction between these inhibitors and human serum albumin (HSA), which plays a crucial role in the drugs' pharmacokinetics and pharmacodynamic properties. The study reveals that the complex formation between FXa inhibitors and HSA occurs through static quenching and affects HSA's fluorescence. The binding constants obtained through different methods show variations, and molecular dynamics simulations support the suspected binding mode.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Toxicology
Supun Wedasingha, Anjana Silva, Sisira Siribaddana, Kanishka Seneviratne, Geoffrey K. Isbister
Summary: This study investigated the diagnostic accuracy of whole blood clotting test and capillary blood clotting test for detecting VICC in viper envenoming in Sri Lanka. WBCT-15 had the best sensitivity for detecting VICC and complete VICC, while CBCT-t had an excellent sensitivity for detecting VICC but poor specificity. The study found higher sensitivity in detecting VICC in Russell's viper bites.
CLINICAL TOXICOLOGY
(2022)
Article
Biology
Vepusha Sathanantham, Lorenzo Alberio, Cedric Bovet, Pierre Fontana, Bernhard Gerber, Lukas Graf, Adriana Mendez, Thomas C. Sauter, Adrian Schmidt, Jan-Dirk Studt, Walter A. Wuillemin, Michael Nagler
Summary: PiCT is proposed as a reliable laboratory test for measuring direct oral anticoagulant drug levels, and it can accurately predict clinically relevant drug concentrations.
Article
Cardiac & Cardiovascular Systems
Youngeun Lee, Ja-Yoon Gu, Hyun Kyung Kim
Summary: Lupus anticoagulant (LA), composed of heterogeneous autoantibodies, is closely associated with thrombotic events. Patients with positive results for both the dilute Russell viper venom time (dRVVT) and silica clotting time (SCT) were found to have a higher risk of developing thrombotic events, along with higher rates of positivity for aCL and aB2GPI antibodies.
JOURNAL OF THROMBOSIS AND THROMBOLYSIS
(2022)
Article
Medicine, General & Internal
Carine Farkh, Syrine Ellouze, Louis Gounelle, Mama Sad Houari, Jerome Duchemin, Valerie Proulle, Michaela Fontenay, Xavier Delavenne, Georges Jourdi
Summary: The DOAC Filter is an easy-to-use device that effectively removes FXa inhibitors from plasma samples, reducing their interference with LA testing in treated patients.
FRONTIERS IN MEDICINE
(2021)
Article
Pharmacology & Pharmacy
D. Pangam, V Jaiswal, P. Dongre
Summary: This study successfully inhibited the activity of Russell's viper venom using chemically synthesized silver nanoparticles, and further analyzed the properties and biocompatibility of the conjugate.
INDIAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Biochemical Research Methods
Parviz Ghezellou, Sven Heiles, Patrik Kadesch, Alireza Ghassempour, Bernhard Spengler
Summary: The snake venom gland is the site for synthesis, storage, and secretion of venom. While its morphology has been studied, the cellular secretory and functional processes are still not fully understood. Using advanced mass spectrometry imaging techniques, lipids and peptides were identified in the venom gland, providing insights into its composition and potential functions.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Chemistry, Medicinal
Juliana Castro-Amorim, Ana Oliveira, Ashis K. Mukherjee, Maria J. Ramos, Pedro A. Fernandes
Summary: In this study, the catalytic mechanism of RVV-X was determined using a density functional theory/molecular mechanics approach. The results showed that the catalytic process occurs in two steps, involving nucleophilic attack and protonation of the substrate. Zn2+ was found to play a unique role in catalysis by lowering the pKa of the Zn2+-bound water molecule.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2023)
Article
Medicine, General & Internal
Aljeirou Alcachupas, Krisverlyn Bellosillo, Wynlee Rhm Catolico, Mark Cullen Davis, Alyssa Diaz, Yvette Karla Doyongan, Reczy Eduarte, Emerald Gersava, Mary Bernadette Intrepido, Candra Carmelli Lavalle, Jeffrey Millan
Summary: The venom of T. flavomaculatus, a snake species found in the Philippines, may have thrombolytic properties. This study conducted in vitro and in vivo experiments to determine the thrombolytic effect of T. flavomaculatus venom on human blood and ferric chloride-induced cardiac thrombosis in mice.
CUREUS JOURNAL OF MEDICAL SCIENCE
(2023)
Article
Pharmacology & Pharmacy
Allison Cid, Megan E. Smetana, Courtney Hebert, Kelci Coe, Jessica M. Smith
Summary: The aim of this study was to evaluate the effect of concomitant use of fluconazole and direct oral anticoagulants (DOACs) on bleeding risk. The study found that patients receiving a DOAC with fluconazole did not have a significantly increased risk for bleeding at 30 days compared to those receiving a DOAC alone after adjusting for confounding variables.
Article
Toxicology
Chuanbin Shen, Ming Liu, Daniel Thomas Mackeigan, Zi Yan Chen, Pingguo Chen, Danielle Karakas, June Li, Peter A. A. Norris, Jiayao Li, Yanling Deng, Chengbo Long, Ren Lai, Heyu Ni
Summary: This study discovered that patients bitten by specific vipers may develop antivenom-resistant and coagulopathy-independent thrombocytopenia. The desialylation-mediated platelet clearance caused by viper envenomation is identified as a novel mechanism of refractory thrombocytopenia, with C-type lectin-like proteins in viper venoms contributing to this condition.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Immunology
Thyago Bispo Leonel, Joel Jose Megale Gabrili, Carla Cristina Squaiella-Baptistao, Trent M. Woodruff, John D. Lambris, Denise V. Tambourgi
Summary: This study analyzed the effects of Bothrops jararaca snake venom on the complement system and innate immunity. The venom was found to activate the complement system and induce inflammatory responses. Inhibition of the complement system may represent a potential therapeutic approach for Bothrops jararaca envenoming.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Infectious Diseases
Frank-Leonel Tianyi, Muhammad Hamza, Saidu B. Abubakar, Jaffer Al Solaiss, Anna Trelfa, Hadiza L. Abdullahi, Garba Iliyasu, Nuhu Mohammed, Suleman A. Mohammed, Nicholas R. Casewell, Robert A. Harrison, David G. Lalloo, Ymkje Stienstra, Abdulrazaq G. Habib
Summary: Bites by the West African carpet viper often lead to a bleeding syndrome. In this study, the researchers analyzed the use of the 20-minute whole blood clotting test (20WBCT) for detecting venom-induced consumptive coagulopathy (VICC) in snakebite patients. The 20WBCT showed a high sensitivity of 87% and a specificity of 50% for detecting VICC. It is a simple and cost-effective bedside test that can be used in the diagnosis of VICC in North-eastern Nigeria.
PLOS NEGLECTED TROPICAL DISEASES
(2023)
Article
Cardiac & Cardiovascular Systems
Asmaa Albuloushi, Megan Rhoten, Julie Kelly, Katelyn W. Sylvester, Jessica Grandoni, Jean M. Connors
Summary: Historically, HIT has been treated with non-heparin parenteral anticoagulants followed by warfarin once platelets recover. Limited data supports the use of DOACs for HIT treatment. This study described DOAC prescribing patterns for HIT, showing that most patients transitioned to DOACs after platelet recovery, with no progression of HIT observed. Apixaban was the most frequently utilized DOAC in this cohort.
JOURNAL OF THROMBOSIS AND THROMBOLYSIS
(2022)
Article
Pharmacology & Pharmacy
Chelsea N. Lopez, Luma Succar, Sara Varnado, Kevin R. Donahue
Summary: This study compared the impact on bleeding rates of a delayed, clinically driven strategy versus a standard per-package-insert strategy for transitioning from a direct oral anticoagulant to a parenteral anticoagulant. The primary endpoint showed a higher incidence of major bleeding in the delayed group compared to the standard group. Patients in both groups who experienced bleeding had a higher severity of illness and higher median DOAC anti-factor Xa concentrations.
JOURNAL OF CLINICAL PHARMACOLOGY
(2021)
Article
Hematology
E. O. Artemenko, S. I. Obydennyi, K. S. Troyanova, G. A. Novichkova, D. Y. Nechipurenko, M. A. Panteleev
Summary: In this study, we investigated the adhesive properties of circulating plasma-derived and platelet-derived microvesicles (MVs) and their potential role in thrombus growth. The results showed that both types of MVs weakly bound fibrinogen but did not stably adhere to immobilized fibrinogen. Moreover, the presence of these MVs did not significantly affect thrombus formation. Therefore, MVs present in the plasma of healthy individuals are unlikely to directly contribute to thrombus formation under arterial flow conditions.
THROMBOSIS RESEARCH
(2024)
Letter
Hematology
Giuseppe Lippi, Camilla Mattiuzzi, Emmanuel J. Favaloro
THROMBOSIS RESEARCH
(2024)
Article
Hematology
Marium Malik, Maha Al-Ghafry, Abraham Haimed, Julia Su, Maribel Lema, Linda Shore-Lessersson, Suchitra S. Acharya
Summary: This study analyzed and evaluated leukemia-associated coagulopathy in newly diagnosed pediatric leukemia patients using CCTs and ROTEM. The results showed that fibrinogen and platelets played a significant role in causing coagulopathies in this population. This suggests the utility of using CCTs and ROTEM in the pediatric population to evaluate hemostatic function and guide blood product replacement.
THROMBOSIS RESEARCH
(2024)
Review
Hematology
Mohammad M. Elahi, Alexandra N. Witt, Edward L. G. Pryzdial, Paul B. McBeth
Summary: Thrombotic disease may be underdiagnosed in microgravity conditions, and the underlying factors are still poorly understood. A case of internal jugular vein thrombosis was recently diagnosed in a low-risk female astronaut, and six out of ten additional crew members showed risk factors for jugular venous flow. Observations in space and studies on Earth suggest that microgravity affects blood flow, coagulation, and vascular function.
THROMBOSIS RESEARCH
(2024)
Review
Hematology
Stian Ingemann-Molden, Christina Krogner Caspersen, Nanna Rolving, Anette Arbjerg Hojen, Frederikus A. Klok, Erik L. Grove, Barbara Cristina Brocki, Jane Andreasen
Summary: This study found that no single PROM covered all aspects considered important by PE patients. PROMs recommended in the ICHOM core set covered most aspects, but worrisome thoughts, hypervigilance around symptoms, and uncertainty of illness were not covered.
THROMBOSIS RESEARCH
(2024)
Article
Hematology
Haomin Yan, Tsutomu Sasaki, Yasufumi Gon, Kumiko Nishiyama, Hideaki Kanki, Hideki Mochizuki
Summary: The incidence of cancer-associated stroke has increased with the prolonged survival times of cancer patients. In this study, the researchers focused on the driver gene KRAS and found that KRAS mutations exacerbated neurological deficits after stroke and promoted tumor growth. They also identified the upregulation of pro-inflammatory responses and the promotion of STAT3 phosphorylation in mice with KRAS mutations. Additionally, the researchers found that colorectal cancer patients with stroke onset within 3 months after cancer diagnosis had a worse prognosis.
THROMBOSIS RESEARCH
(2024)
Letter
Hematology
Tomoki Togashi, Satomi Nagaya, Makiko Meguro-Horike, Haruto Matsumoto, Yuta Imai, Koichi Yamaguchi, Yoshinari Fujii, Haruka Moriya, Yuika Kikuchi, Ibuki Yasuda, Shin-ichi Horike, Eriko Morishita
THROMBOSIS RESEARCH
(2024)
Article
Hematology
Marta Najarro, Winnifer Briceno, Carmen Rodriguez, Alfonso Muriel, Sara Gonzalez, Ana Castillo, Ignacio Jara, Parth Rali, Catalin Toma, Behnood Bikdeli, David Jimenez
Summary: The CPES score is a useful tool in identifying the risk of hemodynamic deterioration in normotensive patients with acute PE, with acceptable C-statistic, excellent specificity, and low positive predictive value.
THROMBOSIS RESEARCH
(2024)
Article
Hematology
Lei Liu, Huifang Xu, Jian Wang, Haiyan Wang, Saisai Ren, Qian Huang, Mingyan Zhang, Hui Zhou, Chunyan Yang, Lu Jia, Yu Huang, Hao Zhang, Yanling Tao, Ying Li, Yanan Min
Summary: This study investigated the gut microbiota metabolites and cytokines, chemokines, and growth factors in patients with ITP and found their association with treatment responsiveness. The findings suggest that imbalanced secretion of cyto(chemo)kins/growth factors and inadequate levels of TMAO are characteristic of patients with ITP.
THROMBOSIS RESEARCH
(2024)
Article
Hematology
Roxanne Mcknight, Mohammad Salameh, Lee Ann Jones, Greta Anton, Lindsay Carter
Summary: This study evaluated the safety of fixed-dose heparin titration boluses in underweight patients with VTE. The results showed that although underweight patients required more frequent holding of heparin, there was no significant increase in bleeding risk.
THROMBOSIS RESEARCH
(2024)