Article
Plant Sciences
Benjamin Selles, Tiphaine Dhalleine, Alexis Boutilliat, Nicolas Rouhier, Jeremy Couturier
Summary: The study found that the cysteine residue in Arabidopsis PIN1At is crucial for its activity, and oxidative modifications of this residue can lead to decreased activity, formation of dimers, sulfinic or sulfonic acid forms, indicating its susceptibility to oxidative stress conditions.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Cell Biology
Jingxiu Xu, Xinan Meng, Qingxian Yang, Jianqin Zhang, Wei Hu, Hongying Fu, Jack Wei Chen, Weirui Ma, Andrew D. Chisholm, Qiming Sun, Suhong Xu
Summary: The study explores how tissue damage activates Rho small GTPases to trigger actin polymerization for wound repair. The research indicates that H2O2-regulated fine-tuning of CDC-42 localization plays a crucial role in wound closure.
Article
Microbiology
Johan O. Ipsen, Katja S. Johansen, Soren Brander
Summary: Lytic polysaccharide monooxygenases (LPMOs) are enzymes that use copper and oxygen to break down stubborn polysaccharides, making them important in industries such as lignocellulosic refineries. A new fluorescence-based assay has been developed to measure LPMO activity, which can detect as little as 1 nM LPMO under optimized conditions. The assay can also be used to identify peroxidase activity.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Yun Lu, Hongtong Chen, Penghe Wang, Jing Pang, Xi Lu, Guoqing Li, Xinxin Hu, Xiukun Wang, Xinyi Yang, Congran Li, Yu Lu, Xuefu You
Summary: The study investigates the modifications of cysteine residues in the proteome of Mycobacterium tuberculosis under oxidative stress, revealing proteins that play a role in maintaining redox homeostasis.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Marie Pacoud, Karine Mandon, Julie Cazareth, Olivier Pierre, Pierre Frendo, Genevieve Alloing
Summary: Reactive oxygen species, such as hydrogen peroxide, play a crucial role in controlling the establishment and functioning of Rhizobium-legume symbiosis. This study used biosensors to measure the intracellular redox state of Sinorhizobium meliloti, a bacterium involved in nitrogen-fixing symbiosis. The results revealed an oxidative shift in the redox status of S. meliloti during bacteroid differentiation. These findings provide new opportunities for studying redox dynamics during N2-fixing symbiosis.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Anthony W. DeMartino, Matthew B. Amdahl, Kaitlin Bocian, Jason J. Rose, Jesus Tejero, Mark T. Gladwin
Summary: The research indicates that the His81 binding equilibrium of cytoglobin is modulated by the formation of a disulfide bridge between Cys38 and Cys83. Experimental results show the presence of at least two kinetically distinct subpopulations in cytoglobin, depending on the thiol oxidation states.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Nina Prescher, Sebastian Haensch, Christiane B. Knobbe-Thomsen, Kai Stuehler, Gereon Poschmann
Summary: CAPG is a redox-sensitive protein with newly identified cysteines that play a role in regulating cell migration, potentially serving as new targets for controlling tumor invasive properties.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
K. Pimkova, M. Jassinskaja, R. Munita, M. Ciesla, N. Guzzi, P. Cao Thi Ngoc, M. Vajrychova, E. Johansson, C. Bellodi, J. Hansson
Summary: Fetal and adult hematopoietic stem and progenitor cells have distinct redox homeostasis, with fetal cells being more susceptible to protein thiol oxidation. The study reveals ontogenic changes to oxidation state of thiols in proteins involved in metabolism and protein homeostasis, as well as thiol oxidation changes in mitochondrial respiration and protein homeostasis during initiation of MLL-rearranged leukemia in fetal hematopoietic stem and progenitor cells.
Article
Physiology
Andonis Karachitos, Wojciech Grabinski, Martyna Baranek, Hanna Kmita
Summary: VDAC serves as a channel protein allowing the exchange of small metabolites and inorganic ions across the mitochondrial outer membrane, participating in regulating mitochondrial and cellular functions. The large diversity of VDAC-encoding genes may lead to physiological plasticity to adapt to stresses impacting mitochondrial function. The occurrence of redox-sensitive VDAC (rsVDAC) is possible in mitochondria, especially prevalent in parasites.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Loes van Dam, Marc Pages-Gallego, Paulien E. Polderman, Robert M. van Es, Boudewijn M. T. Burgering, Harmjan R. Vos, Tobias B. Dansen
Summary: Redox signaling is controlled through the reversible oxidation of cysteine thiols, triggered by H2O2 as a second messenger. Peroxiredoxins are suggested to act as peroxidases, facilitating H2O2-dependent oxidation of redox-regulated proteins. The selective substrate targeting and signaling specificity in peroxiredoxin-based redox relays remain unclear, but evidence supports their role in generating complex cellular signaling responses.
Article
Multidisciplinary Sciences
Christine C. Winterbourn, Alexander Peskin, Torsten Kleffmann, Rafael Radi, Paul E. Pace
Summary: The presence of bicarbonate enhances the inactivation of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) by hydrogen peroxide (H2O2) in a reversible manner. The enhanced inactivation is most likely due to the formation of a more reactive oxidant, peroxy-monocarbonate (HCO4-). GAPDH may facilitate the formation and/or targeting of HCO4- to promote its own inactivation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Yomade Ayodeji Jimoh, Akeem Olalekan Lawal, Ige Joseph Kade, Damilola Moses Olatunde, Oluwafunso Oluwayomi
Summary: The study found that diphenyl diselenide (DPDSe) can reduce the effects of diesel exhaust particle (DEP)-induced neurotoxicity in rats by decreasing oxidative stress and inflammation. DPDSe not only reduced the production of oxidative reaction products, but also increased the activity of antioxidant enzymes and improved the abnormal expression of related genes. These findings suggest that DPDSe may be a promising therapeutic candidate for DEP-induced neurotoxicity.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Nutrition & Dietetics
Konstantinos Papanikolaou, Athanasios Z. Jamurtas, Athanasios Poulios, Panagiotis Tsimeas, Dimitrios Draganidis, Nikos V. Margaritelis, Ioannis Baloyiannis, Constantinos Papadopoulos, Apostolos Sovatzidis, Chariklia K. Deli, Anastasia Rosvoglou, Kalliopi Georgakouli, Theofanis Tzatzakis, Michalis G. Nikolaidis, Ioannis G. Fatouros
Summary: This study investigated the association between dietary nutrient intake, physical fitness, and redox status in erythrocytes and skeletal muscle. The results showed that dietary cysteine intake was positively correlated with glutathione levels and glutathione reductase activity, and negatively correlated with protein carbonyls in erythrocytes. Musculoskeletal fitness and physical activity levels were related to redox biomarkers in skeletal muscle. These findings highlight the importance of cysteine intake for optimal redox regulation and suggest that musculoskeletal fitness and physical activity may influence antioxidant capacity in skeletal muscle.
EUROPEAN JOURNAL OF NUTRITION
(2023)
Review
Biochemistry & Molecular Biology
Luis Eduardo S. Netto, Luciana Elena S. F. Machado
Summary: Protein phosphorylation is an important post-translational modification involved in cell signalling. This review focuses on the biochemical and structural features of protein tyrosine phosphatases (PTPs) and their regulation by reversible oxidation. The authors discuss the oxidation products of PTPs and propose possible mechanisms for the apparent contradiction between biological and chemical redox aspects of PTPs.
Article
Chemistry, Analytical
Islam M. Mostafa, Hongzhan Liu, Saima Hanif, Muhammad Rehan Hasan Shah Gilani, Yiran Guan, Guobao Xu
Summary: A novel electrochemical probe has been developed for the selective determination of biothiols, synthesized through a one-step reaction with high sensitivity and excellent recoveries for cysteine, glutathione, and homocysteine. The probe uses 2,4-dinitrobenzenesulfonyl moiety as a selective recognition group and can detect biothiols in human plasma samples with a high degree of accuracy.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Sabrina Romano, Flavio Di Giacinto, Aniello Primiano, Jacopo Gervasoni, Alberto Mazzini, Massimiliano Papi, Andrea Urbani, Annalucia Serafino, Marco De Spirito, Ewa K. Krasnowska, Gabriele Ciasca
Summary: FTIR spectroscopy is an effective method for studying the differences in cancer-derived EXOs from different cell phenotypes, providing possibilities for diagnosis. The spectral features of EXOs derived from different cell phenotypes in the infrared range can be used to monitor cell changes effectively.
ANALYTICA CHIMICA ACTA
(2022)
Review
Chemistry, Physical
Benedetta Niccolini, Valentina Palmieri, Marco De Spirito, Massimiliano Papi
Summary: Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease characterized by motor neuron degeneration, and deregulation of miRNAs is critical for its onset. Recent studies have focused on the design of delivery systems, such as graphene, to correct aberrant miRNA expression, providing a primer for future research in this field.
Article
Biochemistry & Molecular Biology
Giordano Perini, Enrico Rosa, Ginevra Friggeri, Lorena Di Pietro, Marta Barba, Ornella Parolini, Gabriele Ciasca, Chiara Moriconi, Massimiliano Papi, Marco De Spirito, Valentina Palmieri
Summary: This study presents an image analysis software, INSIDIA 2.0, for high-throughput analysis of in vitro 3D cancer cell spheroids. It allows advanced parameterization and quantification of the effects of nanoparticle photothermal therapy on glioblastoma and pancreatic cancer spheroids. INSIDIA 2.0 analysis revealed two types of effects, which can be measured using quantitative parameters.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Manuela Zonfrillo, Federica Andreola, Ewa K. Krasnowska, Gianluca Sferrazza, Pasquale Pierimarchi, Annalucia Serafino
Summary: Eucalyptus essential oil and its major component eucalyptol have immunostimulatory effects, enhancing the phagocytic activity of macrophages and promoting pathogen clearance. Additionally, eucalyptus essential oil stimulates the formation of podosomes and increases the expression of podosome components. These findings provide further evidence of the multiple properties of eucalyptus essential oil, including immune activation, in addition to its known antimicrobial and anti-inflammatory effects.
Article
Chemistry, Analytical
Cassandra Serantoni, Giovanna Zimatore, Giada Bianchetti, Alessio Abeltino, Marco De Spirito, Giuseppe Maulucci
Summary: The VO(2)max index is of significant importance to overall health, and the use of wearables can estimate it but lacks detailed analysis. This study presents a multiple modality biosignal processing method for real-time investigation of heartbeat dynamics in response to external energy demand during running sessions. The analysis identifies dynamic regimes that increase with VO(2)max and the emergence of neuromuscular fatigue, providing personalized feedback for improving fitness level and reducing cardiovascular risk.
Article
Materials Science, Textiles
Flavio De Maio, Giulia Santarelli, Valentina Palmieri, Giordano Perini, Alessandro Salustri, Ivana Palucci, Giovanni Delli Carpini, Alberto Augello, Maurizio Sanguinetti, Marco De Spirito, Michela Sali, Giovanni Delogu, Massimiliano Papi
Summary: Respiratory tract infections are the leading cause of death from infectious diseases globally, with COVID-19 adding to the burden of existing respiratory tract infections like tuberculosis. Graphene and curcumin can be used to create fabric coatings on cotton and polyester, providing personal protective equipment that is resistant to infectious agents.
JOURNAL OF NATURAL FIBERS
(2023)
Article
Biochemistry & Molecular Biology
Giada Bianchetti, Maria Elisabetta Clementi, Beatrice Sampaolese, Cassandra Serantoni, Alessio Abeltino, Marco De Spirito, Shlomo Sasson, Giuseppe Maulucci
Summary: Diabetes-induced oxidative stress causes vascular complications, particularly diabetic retinopathy (DR), which is attributed to harmful oxidative stress events in the blood-retinal barrier (BRB). Docosahexaenoic acid (DHA) has therapeutic potential against oxidative damage in retinal pigment epithelium cells (ARPE-19) and activates the Nrf2/Nqo1 signaling cascade to promote antioxidant response. This study investigates the modulation of lipid metabolism by high-glucose (HG) conditions on ARPE-19 cells and demonstrates that DHA ameliorates lipid metabolism and reduces ROS production under HG conditions. Targeting lipid metabolism pathways may offer new treatment strategies for DR.
Article
Chemistry, Physical
Valentina Palmieri, Francesco Amato, Andrea Giacomo Marrani, Ginevra Friggeri, Giordano Perini, Alberto Augello, Marco De Spirito, Massimiliano Papi
Summary: Graphene Oxide (GO) is an oxidized form of graphene that contains various oxygen functional groups. The solubility of GO in water makes it an ideal material in the biomedical field, but the synthesis methods used can affect the balance of oxygen groups and the impact on cells. Spectroscopic techniques are commonly used to characterize GO, but they have limitations in indicating its reactivity with polymers or biological media. In this study, a colorimetric method based on GO's reactivity with copper ions was developed to evaluate the oxidation degree and accessibility of polymeric samples. This technique will be crucial for scaffold characterization in tissue engineering and studying interactions between GO-related materials and biological entities.
APPLIED SURFACE SCIENCE
(2023)
Article
Chemistry, Physical
Massimiliano Papi, Marco De Spirito, Valentina Palmieri
Summary: The COVID-19 pandemic has prompted collaboration between nanotechnology scientists, industry stakeholders, and clinicians to find solutions for SARS-CoV-2 infections. Carbon-based materials (CBM) like graphene and carbon nanotubes have shown potential in viral research due to their unique effects on microorganisms and immune interaction. This review explores the interaction of CBM with SARS-CoV-2, including their physical and chemical properties, known interactions with viral components, and potential applications in prevention, treatment, and diagnostics.
Article
Biochemistry & Molecular Biology
Alex De Gregorio, Annalucia Serafino, Ewa Krystyna Krasnowska, Fabiana Superti, Maria Rosa Di Fazio, Maria Pia Fuggetta, Ivano Hammarberg Ferri, Carla Fiorentini
Summary: Chemotherapy and gut inflammation can lead to increased intestinal permeability, but the gut microbiota may play a role in maintaining intestinal barrier integrity. This study found that L. fermentum ME-3 can prevent the increase in intestinal permeability caused by chemotherapy or inflammation and reduce the release of inflammatory cytokines. These findings suggest that L. fermentum ME-3 may be a valuable therapeutic tool for preventing leaky gut syndrome.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Nutrition & Dietetics
Alessio Abeltino, Giada Bianchetti, Cassandra Serantoni, Alessia Riente, Marco De Spirito, Giuseppe Maulucci
Summary: Nutrition is a crucial aspect of medicine, affecting various health conditions. Digital medicine utilizes digital twins, which are replicas of human physiology, to prevent and treat diseases. This study compared different models for the deployment of Personalized Metabolic Avatars, with GRUs and LSTMs showing the best predictive performance and acceptable computational times. The Transformer model did not significantly improve predictive performance but increased computational time, while the SARIMAX model had the best computational time but worst predictive performance.
Article
Multidisciplinary Sciences
Davide Pierangeli, Giordano Perini, Valentina Palmieri, Ivana Grecco, Ginevra Friggeri, Marco De Spirito, Massimiliano Papi, Eugenio DelRe, Claudio Conti
Summary: The authors discovered that tumor-cell spheroids exhibit optical rogue waves under randomly modulated laser beam illumination. The intensity of transmitted light follows a Weibull statistical distribution, with extreme events corresponding to localized optical modes propagating within the cell network. These nonlinear optical filaments form high-transmission channels and can be used to achieve controlled temperature increase. This study sheds light on optical propagation in biological aggregates and demonstrates the potential of using rogue waves in biomedical applications.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Francesco Amato, Giordano Perini, Ginevra Friggeri, Alberto Augello, Alessandro Motta, Leonardo Giaccari, Robertino Zanoni, Marco De Spirito, Valentina Palmieri, Andrea Giacomo Marrani, Massimiliano Papi
Summary: Graphene oxide and reduced graphene oxide are widely used carbon nanomaterials in biomedicine. However, the hydrophobic behavior of reduced graphene oxide limits its stability in biological media. In this study, sodium ascorbate is used as a reducing agent to prepare reduced graphene oxide with improved stability and suitability for applications in cell culture media.
ADVANCED MATERIALS INTERFACES
(2023)
Article
Chemistry, Analytical
Alessia Riente, Alessio Abeltino, Cassandra Serantoni, Giada Bianchetti, Marco De Spirito, Stefano Capezzone, Rosita Esposito, Giuseppe Maulucci
Summary: We developed a non-invasive device that evaluates personalized chewing styles by analyzing various aspects. The device provides valuable insights into personalized chewing profiles and could modify unhealthy chewing habits.