Article
Multidisciplinary Sciences
Morgane Le Rolle, Filippo Massa, Pam Siggers, Laurent Turchi, Agnes Loubat, Bon-Kyoung Koo, Hans Clevers, Andy Greenfield, Andreas Schedl, Marie-Christine Chaboissier, Anne-Amandine Chassot
Summary: This study shows that germ cell-intrinsic beta-catenin activity in mouse embryonic ovaries is essential for maintaining pluripotency and its repression is crucial for differentiation and meiosis entry. The interaction of beta-catenin with the pluripotent-associated factor POU5F1 in the nucleus is associated with germ cell pluripotency, and its exit from the nucleus correlates with germ cell differentiation. These findings highlight the role of beta-catenin as a central gatekeeper in ovarian differentiation and gametogenesis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Haifeng Liu, Hanyong Jin, Guenhwi Kim, Jeehyeon Bae
Summary: This study demonstrates that BPA at very low concentrations can increase expression of FOXL2, leading to enhanced levels of CYP19A1 and production of estradiol in human ovarian granulosa cells. The molecular signaling cascade involving BPA-CTNNB1-FOXL2-CYP19A1-E2 contributes to the endocrine-disrupting activities of BPA in human ovarian granulosa cells.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Review
Reproductive Biology
Xiaxia Man, Zhentong Wei, Baogang Wang, Wanying Li, Lingling Tong, Liang Guo, Songling Zhang
Summary: Microcystic stromal tumor (MCST) of the ovary is an extremely rare subtype of sex cord-stromal neoplasm, with unique histologic and immunohistochemical profiles and genetic alterations identified in a case of ovarian MCST with omental metastasis.
JOURNAL OF OVARIAN RESEARCH
(2021)
Article
Agriculture, Dairy & Animal Science
E. M. Ferranti, B. H. Aloqaily, C. A. Gifford, K. K. Forrest, C. A. Loest, J. C. Wenzel, J. A. Hernandez Gifford
Summary: This study demonstrated that low doses of LPS can affect follicular dynamics and hormone levels, potentially modulated through the regulation of beta-catenin on estradiol concentrations.
DOMESTIC ANIMAL ENDOCRINOLOGY
(2022)
Review
Reproductive Biology
Deepa Bhartiya, Hiren Patel, Ankita Kaushik, Pushpa Singh, Diksha Sharma
Summary: This study reveals that stem/progenitor cells in adult tissues express FSH receptor (FSHR) and directly respond to FSH through FSHR-3, leading to a paradigm shift in the field of FSH-FSHR biology. These findings explain the expression of FSHR on cancer cells in multiple organs and provide straightforward explanations for various existing conundrums, including extragonadal expression of FSHR.
JOURNAL OF OVARIAN RESEARCH
(2021)
Article
Obstetrics & Gynecology
Mandeep Kaur, Sukhjashanpreet Singh, Ratneev Kaur, Archana Beri, Anupam Kaur
Summary: This study found an association between FSHR gene variants and clinical features of PCOS, but no significant correlation with direct disease risk.
REPRODUCTIVE SCIENCES
(2023)
Review
Multidisciplinary Sciences
An-Hao Liu, Zi-Qing Zhao, Xiu-Wen Li, Bin Xu, Run-Fang Yu, Shao-Fen Ye, Zhi-Hui Miao, Li-Li Huang, Bin-Bin Gao, Hai-Yan Lai, Jia-Hui Lin, Ming-Zhu Ye, Xiu-Min Huang, Zheng-Jin Liu, Xun Chen
Summary: This case report describes an unusual case of primary ovarian SPN and provides an up-to-date review of SPN-O. It suggests the use of a minimum combination of imaging methods and immunohistochemistry staining for SPN-O, which may be beneficial in clinical practice.
Review
Cell Biology
Carlos Parra-Herran, W. Glenn McCluggage
Summary: Microcystic stromal tumour (MST) is a rare, usually benign, ovarian neoplasm with distinctive morphological features and characteristic immunohistochemical expression. It can also be associated with familial adenomatous polyposis.
Article
Cell Biology
Huibin Zhang, Jinglin Wang, Fan Xie, Yangguang Liu, Mengyao Qiu, Zheng Han, Yueyun Ding, Xianrui Zheng, Zongjun Yin, Xiaodong Zhang
Summary: This study investigated the effects of resveratrol (RSV) on the apoptosis of porcine ovarian granulosa cells (POGCs) through miRNA regulation. The results showed that RSV promoted POGCs apoptosis by regulating the expression of miRNAs, and also provided insights into the role of miRNAs combined with RSV in ovarian granulosa cell development in pigs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Medicine, General & Internal
Lasma Lidaka, Laine Bekere, Adele Rota, Jekaterina Isakova, Gunta Lazdane, Anda Kivite-Urtane, Iveta Dzivite-Krisane, Inga Kempa, Zane Dobele, Linda Gailite
Summary: This study investigated single nucleotide variants (SNVs) in key genes of adolescent PCOS patients and found associations between SNVs ESR2 rs4986938 and LHCGR rs2293275 with phenotypic characteristics of PCOS. However, no significant differences in genotype distributions were found among PCOS patients, risk patients, and healthy controls. Further studies with larger cohorts and follow-up through adulthood are needed to fully understand the influence of these SNVs on PCOS development and symptoms.
Article
Marine & Freshwater Biology
Maria A. Risalde, Ana Ma Molina, Antonio J. Lora, Nahum Ayala, Jose C. Gomez-Villamandos, Ma Rosario Moyano
Summary: This study evaluated the expression of cyp19a aromatase in zebrafish after exposure to different concentrations of BPA, finding that these aromatases are overexpressed and could be good biomarkers for identifying early effects of low BPA concentrations. The study is the first to localize and quantify the expression of cyp19a1a and cyp19a1b in the cells of brain and ovary after fish exposure to BPA.
AQUATIC TOXICOLOGY
(2021)
Article
Multidisciplinary Sciences
Mahnaz Zarein, Asghar Zarban, Hamed Shoorei, Mehdi Gharekhani, Mohammadmehdi Hassanzadeh-Taheri
Summary: The study aimed to examine the effects of the antioxidant hesperidin on ovarian damage and toxicity caused by malathion. The results showed that malathion exposure led to abnormalities in ovarian architecture and reduced follicular counts, while hesperidin mitigated the negative effects of malathion on ovarian tissues.
Article
Obstetrics & Gynecology
Zuzana Fabova, Barbora Loncova, Miroslav Bauer, Alexander Sirotkin
Summary: This study aimed to investigate the effects of miR-34a mimics and miR-34a inhibitor, both alone and in combination, on the basic functions of ovarian cells cultured with and without FSH, while also examining the impact of FSH on endogenous miR-34a expression. The findings suggest that miR-34a is involved in the control of ovarian functions and that there is a synergistic relationship between miR-34a and FSH in their actions on ovarian cell functions. Furthermore, FSH can promote miR-34a expression, and miR-34a mimics can increase the occurrence of FSHR and modify the effects of FSH on ovarian cells.
REPRODUCTIVE SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Fen Ji, Chao Feng, Jie Qin, Chong Wang, Dongming Zhang, Libo Su, Wenwen Wang, Mengtian Zhang, Hong Li, Longbing Ma, Weicheng Lu, Changmei Liu, Zhaoqian Teng, Baoyang Hu, Fengzeng Jian, Jingdun Xie, Jianwei Jiao
Summary: The deletion of Pd1 in the brain leads to abnormal cortical neurogenesis and depressive-like behaviors. Pd1 regulates neurogenesis by targeting Pax3 through the β-catenin signaling pathway. Furthermore, Pd1 also plays a similar role in the proliferation and differentiation of human neural progenitor cells (hNPCs).
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Developmental Biology
Haiming Yu, Xiaoqing Zhou, Yujing Zhang, Kexin Wen, Zhengli Yan, Hu Fu, Yongfei Zhu
Summary: Exposure to flutamide during pregnancy resulted in lower bodyweight, ovarian and uterine weights in female offspring, along with histopathological changes such as fewer developed follicles. Flutamide increased the expression of UPR members and autophagy- and apoptosis-related genes, suggesting a potential mechanism of endoplasmic reticulum stress-induced damage in the uterus and ovaries of offspring.
REPRODUCTION FERTILITY AND DEVELOPMENT
(2021)
Article
Agriculture, Dairy & Animal Science
M. Grzesiak, K. Knapczyk-Stwora, M. R. Luck, A. Mobasheri, M. Slomczynska
REPRODUCTION IN DOMESTIC ANIMALS
(2016)
Article
Agriculture, Dairy & Animal Science
M. Nowak, M. Grzesiak, N. Saito, M. Kwasniewska, A. Sechman, A. Hrabia
REPRODUCTION IN DOMESTIC ANIMALS
(2017)
Article
Agriculture, Dairy & Animal Science
M. Grzesiak, A. Michalik, A. Rak, K. Knapczyk-Stwora, A. Pieczonka
DOMESTIC ANIMAL ENDOCRINOLOGY
(2018)
Article
Reproductive Biology
Malgorzata Grzesiak, Ewa Waszkiewicz, Magdalena Wojtas, Kinga Kowalik, Anita Franczak
Article
Agriculture, Dairy & Animal Science
P. Witek, M. Grzesiak, M. Koziorowski, M. Slomczynska, K. Knapczyk-Stwora
DOMESTIC ANIMAL ENDOCRINOLOGY
(2020)
Article
Reproductive Biology
Katarzyna Knapczyk-Stwora, Malgorzata Grzesiak, Patrycja Witek, Malgorzata Duda, Marek Koziorowski, Maria Slomczynska
Article
Agriculture, Dairy & Animal Science
Malgorzata Grzesiak, Magdalena Socha, Anna Hrabia
Summary: This study found that the concentration of 25OHD(3) in the fluid of follicular and follicular lutein cysts of sows was significantly lower than in preovulatory follicles. The protein abundance of vitamin D metabolic enzymes (CYP27B1 and CYP24A1) in the cyst wall was also decreased compared to preovulatory follicles, suggesting a potential association between altered VD metabolism and the formation of ovarian cysts in sows.
REPRODUCTION IN DOMESTIC ANIMALS
(2021)
Article
Reproductive Biology
Malgorzata Grzesiak, Klaudia Kapusta, Kinga Kaminska, Sylwia Palka, Michal Kmiecik, Joanna Zubel-Lojek
Summary: The study investigated the effects of dietary supplementation with nettle or fenugreek on folliculogenesis and steroidogenesis in juvenile rabbit ovaries. Nettle decreased primordial and early antral follicles, while increasing primary follicles, and fenugreek increased primary and antral follicles but decreased early antral follicles. Fenugreek also targeted steroidogenic enzymes, affecting estradiol biosynthesis and plasma concentration, potentially influencing sexual maturation in rabbits.
Article
Biochemistry & Molecular Biology
Malgorzata Grzesiak, Kinga Kaminska, Aleksandra Bodzioch, Ewa M. Drzewiecka, Anita Franczak, Katarzyna Knapczyk-Stwora
Summary: This study reveals the presence of vitamin D-3 metabolism in the uterus of pigs, with both endometrium and myometrium capable of synthesizing vitamin D-3, while only the endometrium is involved in its metabolism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Reproductive Biology
Malgorzata Grzesiak, Kinga Kaminska, Katarzyna Knapczyk-Stwora, Anna Hrabia
Summary: This study identified altered expression of the MMP/TIMP system in sow follicular cysts, suggesting increased ECM degradation and implicating these components in the pathogenesis of porcine ovarian cysts through ECM remodeling.
Review
Biochemistry & Molecular Biology
Malgorzata Grzesiak, Marcelina Tchurzyk, Magdalena Socha, Andrzej Sechman, Anna Hrabia
Summary: Recent studies have shown that vitamin D-3 is crucial for female reproductive processes. Deficiency can lead to reproductive disturbances such as ovarian cysts. While extensively studied in humans and rodents, the association with female reproductive function in farm animals, birds, and fish has been rarely summarized.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
Magdalena Socha, Joanna Szczygiel, Jaroslaw Chyb, Ewa Drag-Kozak, Miroslawa Sokolowska-Mikolajczyk, Elzbieta Brzuska, Anna Pecio, Malgorzata Grzesiak
Summary: This study investigated the effect of Roundup herbicide on the reproductive parameters of crucian carp. The results suggest that exposure to this herbicide may adversely affect oocyte maturation and egg quality, leading to reproductive disorders in fish.
Article
Reproductive Biology
Kinga Kaminska, Kasun Godakumara, Bianka Swiderska, Agata Malinowska, Getnet Midekessa, Kamila Sofinska, Jakub Barbasz, Alireza Fazeli, Malgorzata Grzesiak
Summary: Extracellular vesicles (EVs) are membrane-bound nanoparticles released by different cell types, playing a crucial role in intercellular communication by carrying various biomolecular compounds. This study assessed the use of size-exclusion chromatography (SEC) for effectively isolating EVs from porcine ovarian follicular fluid. The characterization of EVs was performed using multiple techniques, and the results showed that SEC is an effective method for isolating and studying EVs from porcine follicular fluid, providing opportunities for further functional analyses such as proteomics.
Article
Cell Biology
Malgorzata Grzesiak, Gabriela Burzawa, Patrycja Kurowska, Klaudia Blaszczyk, Agata Szlaga, Anna Blasiak, Andrzej Sechman, Agnieszka Rak
Summary: In this study, differences in VD3 metabolism in the ovary and periovarian adipose tissue of PCOS rats were investigated. The reduced concentration of 1,25(OH)(2)D-3 in these tissues suggest their involvement in VD3 deficiency in PCOS and potential implications in PCOS pathogenesis.
HISTOCHEMISTRY AND CELL BIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
M. Grzesiak, K. Knapczyk-Stwora, M. Slomczynska
DOMESTIC ANIMAL ENDOCRINOLOGY
(2017)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.