Article
Agriculture, Dairy & Animal Science
Margarida Fernandes, Pablo Rodriguez Hernandez, Joao Simoes, Joao Pedro Barbas
Summary: The study revealed that the egg yolk-based semen extender performed better in cryopreserving semen from Merino rams. However, there were significant interactions between different semen extenders, semen processing, and collection months on sperm parameters. Further research is needed to address the causes of these interaction effects and improve semen extenders.
Article
Agriculture, Dairy & Animal Science
James D. Gillis, Linda M. Penfold, Natalie D. Mylniczenko
Summary: This study explored the reproductive biology and sperm cryopreservation of southern stingrays, finding correlations between plasma testosterone levels and semen quality, as well as the potential benefits of a slow freezing method in improving post-thaw sperm survival.
Article
Biology
Guy Shrem, Liat Azani, Ido Feferkorn, Tamar Listovsky, Sofia Hussaini, Benjamin Farber, Michael H. Dahan, Mali Salmon-Divon
Summary: The purpose of this study was to examine how different types of cancer affect semen quality. The results showed that all types of cancer significantly impaired sperm quality parameters.
Review
Agriculture, Dairy & Animal Science
Mohammed S. Liman, Abubeker Hassen, Lyndy J. McGaw, Peter Sutovsky, Dietmar E. Holm
Summary: This review examined the impact of semen cryopreservation and tannins on sperm function, highlighting the role of tannins in combating reactive oxygen species and promoting successful fertilization. Additionally, the effects of tannin extract feed supplementation on male fertility were investigated, and the potential benefits of tannins as additives in semen extenders were explored.
Article
Agriculture, Dairy & Animal Science
Shaimaa K. Hamad, Ahmed M. Elomda, Yanyan Sun, Yunlei Li, Yunhe Zong, Jilan Chen, Ahmed O. Abbas, Farid K. R. Stino, Ali Nazmi, Gamal M. K. Mehaisen
Summary: This study aims to evaluate the impact of different concentrations (3%, 6%, or 9%) of dimethylacetamide (DMA) as a cryoprotectant on post-thawed sperm quality, motility, antioxidant-biomarkers, and the expression of anti-freeze related genes. The results showed that 3% DMA group maintained higher post-thawed sperm quality compared to the other tested groups, with improvements in motility, viability, and antioxidant enzyme activity. Additionally, certain anti-freeze related genes were upregulated in the 3% DMA group.
Article
Reproductive Biology
Xing Zhang, Song-Mao Guo, Da-Wei Zhu, Yu Li, Fei Wen, Ming Xian, Zhang-Tao Hu, Qian-Long Zou, Li-Kun Zhang, Yu-Lin Chen, Jian-Hong Hu
Summary: This study investigated the effect of metformin on sheep semen cryopreservation and identified the most effective concentration as 1.0 mmol/L. The results demonstrated that metformin significantly improved sperm quality, reduced oxidative stress, and enhanced sperm mitochondrial function and fertilization capacity through the AMPK pathway.
Review
Agriculture, Dairy & Animal Science
Mohsen Sharafi, Seyyed Mohsen Borghei-Rad, Maryam Hezavehei, Abdolhossein Shahverdi, James D. Benson
Summary: This comprehensive review examines the mechanisms of damage caused by cryopreservation and highlights classical and novel strategies to reduce this damage and improve sperm survival. Cryopreservation is an important method for preserving germplasm in various fields, but the current methods only yield about 50% viable spermatozoa after cryopreservation. Various sperm components, including the plasma membrane, nucleus, mitochondria, proteins, mRNAs, and microRNAs, can be damaged during the process. Conventional strategies using chemical additives have been employed, but they are not completely effective in preventing damage.
Article
Veterinary Sciences
Zainal Zahari Zainuddin, Symphorosa Sipangkui, Mohd Farqhan Kelana, Yap Keng Chee, Mohamed Reza Mohamed Tarmizi, Pierre Comizzoli
Summary: The Arctic Lab aims to study ancient climate change and ancient earthquakes using InSAR technology. They are particularly concerned about the rapid melting of the ice caps in Greenland. By researching these changes, they hope to better predict future climate change trends.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
Cristina Palacin-Martinez, Mercedes Alvarez, Rafael Montes-Garrido, Marta Neila-Montero, Luis Anel-Lopez, Paulino de Paz, Luis Anel, Marta F. Riesco
Summary: Sperm cryopreservation plays a crucial role in optimizing artificial insemination protocols for rams. Previous efforts to improve ram sperm cryopreservation protocols have focused on different methods, such as using different cryoprotectants, antioxidants, or freezing-thawing rates. However, the influence of male sexual regimes on freezeability has not been studied until now. In this study, a multiparametric analysis was conducted to evaluate the quality of cooled and frozen-thawed semen after different sperm collection frequencies. The results showed that semen quality significantly decreased in the groups with zero and ten collections per week compared to the group with four collections per week. Additionally, the apoptosis rate significantly increased in the group with ten collections per week. Enzyme activity related to redox balance, such as glutathione peroxidase and superoxide dismutase, also showed significant decreases in the group with ten collections per week. This study highlights the importance of considering male sexual regimes in alternative strategies for sperm cryopreservation in order to improve frozen-thawed sperm quality.
Article
Biology
Gianluca Paventi, Michele Di Iorio, Giusy Rusco, Anatoly P. Sobolev, Silvia Cerolini, Emanuele Antenucci, Mattia Spano, Luisa Mannina, Nicolaia Iaffaldano
Summary: Semen cryopreservation is crucial for preserving biodiversity, but in avian species like turkeys, the process leads to a significant reduction in sperm quality. Using NMR technology, this study analyzed the metabolic changes in turkey sperm during cryopreservation, revealing differences in the levels of amino acids, water-soluble compounds, and lipids between fresh and frozen-thawed samples.
Article
Agriculture, Dairy & Animal Science
Martina Colombo, Maria Giorgia Morselli, Giulia Franchi, Sabine Schafer-Somi, Gaia Cecilia Luvoni
Summary: The aim of this study was to evaluate the possibility of freezing dog semen after 24 or 48 hours of cooled transport to an equipped laboratory when semen collection was performed in the field. The results showed that there were no significant differences among the frozen groups, regardless of freezing time or extender used. However, motility at thawing decreased at 48 hours. Therefore, freezing after cooled transport could be a good option for long-term preservation of dog semen, especially if the transport can be organized within 24 hours.
Article
Fisheries
Sen Yang, Zhilu Zeng, Xinghan Chen, Bin Fan, Zining Meng
Summary: This study aimed to assess the effect of melatonin addition on post-thaw sperm function of giant grouper. The results showed that melatonin improved the total motility, progressive motility, mitochondrial membrane potential, and velocity of frozen-thawed sperm. Melatonin also reduced apoptotic-like changes, increased sperm viability, and decreased DNA damage. Additionally, melatonin supplementation improved the fertility and hatching rate of cryopreserved giant grouper sperm, with a recommended concentration of 1-2 μM in the cryomedium.
AQUACULTURE REPORTS
(2023)
Article
Agriculture, Dairy & Animal Science
Abouzar Najafi, Hossein Daghigh-Kia, Mahdieh Mehdipour, Hossein Mohammadi, Hamed Hamishehkar
Summary: Antioxidant nanoparticles such as γ-oryzanol nanoparticles have a positive impact on the quality and fertility of thawed rooster sperm, improving motility, plasma membrane functionality, mitochondrial activity, and reducing apoptosis and oxidative stress.
Article
Agriculture, Dairy & Animal Science
Fernanda Gabriela Fumuso, Mariana Lucia Bertuzzi, Nicolas Velasquez Gonzalez, Marcelo H. Miragaya, Maria Ignacia Carretero
Summary: The study found that there was no clear superiority between using a single or a combination of permeable cryoprotectants to freeze llama semen. After equilibration, total sperm motility and live sperm with intact acrosomes remained unchanged. However, a significant decrease in sperm quality was observed after thawing, including in functional membrane percentage, motile sperm, and live sperm with intact acrosomes.
REPRODUCTION IN DOMESTIC ANIMALS
(2021)
Article
Veterinary Sciences
Marcelo Martinez-Barbitta, Claudio Rivera Salinas
Summary: Various tools and methodologies have been developed in modern biotechnology to optimize the conservation of canine semen and improve reproductive outcomes. Sperm activators have been used to enhance sperm survival and quality, with the goal of increasing fertility. The use of sperm activators prior to artificial insemination has been shown to significantly improve the quality of chilled semen.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Andrology
F. C. Almeida, S. V. Silva, H. M. Souza, W. A. Gomes, J. A. C. Lima Filho, A. A. Wicke, A. M. Batista, M. M. P. Guerra
Article
Reproductive Biology
Bruna S. P. Oliveira, Joana A. S. Costa, Elizabete T. Gomes, Diogo M. F. Silva, Sandra M. Torres, Pedro L. J. Monteiro, Antonio S. Santos Filho, Maria Madalena P. Guerra, Gustavo F. Carneiro, Aurea Wischral, Andre M. Batista
Article
Fisheries
Thais Castelo-Branco, Maria Madalena Pessoa Guerra, Roberta Soares, Silvio Peixoto
AQUACULTURE INTERNATIONAL
(2018)
Article
Veterinary Sciences
L. C. P. Arruda, R. A. J. Araujo Silva, M. M. Monteiro, R. P. F. Silva, A. S. Oliveira, F. C. C. Mergulhao, P. L. J. Monteiro, A. M. Batista, M. M. P. Guerra
ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA
(2018)
Article
Agriculture, Dairy & Animal Science
Elizabete T. Gomes, Joana Amelia S. Costa, Diogo Manoel F. Silva, Wasim Al Shebli, Marina L. Azevedo, Pedro L. J. Monteiro, Robespierre A. J. Araujo Silva, Antonio S. Santos Filho, Maria Madalena P. Guerra, Claudio Coutinho Bartolomeu, Aurea Wischral, Andre M. Batista
REPRODUCTION IN DOMESTIC ANIMALS
(2018)
Article
Veterinary Sciences
E. C. B. Silva, L. C. P. Arruda, J. I. T. Vieira, P. C. Soares, M. M. P. Guerra
ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA
(2019)
Article
Agriculture, Dairy & Animal Science
Mine Saraiva de Oliveira, Diogo Ribeiro Camara, Andre Mariano Batista, Robespierre Augusto Joaquim Aradjo Silva, Lucia Cristina Pereira Arruda, Millena Maria Monteiro, Joana Amelia de Senna Costa, Maria Madalena Pessoa Guerra
SMALL RUMINANT RESEARCH
(2019)
Article
Agriculture, Dairy & Animal Science
H. M. Souza, L. C. P. Arruda, M. M. Monteiro, I. H. A. V. Nery, R. A. J. Araujo Silva, A. S. Oliveira, A. M. Batista, M. M. P. Guerra
SOUTH AFRICAN JOURNAL OF ANIMAL SCIENCE
(2019)
Article
Veterinary Sciences
E. C. B. Silva, J. I. T. Vieira, I. H. A. Nery, R. A. J. Araujo Silva, V. F. M. H. Lima, M. M. P. Guerra
ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA
(2020)
Article
Cell Biology
Igor H. A. Nery, Robespierre A. J. Araujo Silva, Helder M. Souza, Lucia C. P. Arruda, Millena M. Monteiro, Desiree C. M. Seal, Girliane R. Silva, Tania M. S. Silva, Gustavo F. Carneiro, Andre M. Batista, Diogo R. Camara, Maria Madalena Pessoa Guerra
BIOPRESERVATION AND BIOBANKING
(2020)
Article
Veterinary Sciences
Rodrigo Alves Monteiro, Rodolfo Marinho Cunha, Maria Madalena Pessoa Guerra, Valdir Morais de Almeida, Carlos Enrique Pena-Alfaro, Sildivane Valcacia Silva
Summary: The aim of this study was to test equine semen cryopreservation techniques for the conservation of donkey germplasm. The study found that centrifugation is the most suitable technique to remove donkey seminal plasma, and both automated and conventional freezing methods can be used for donkey semen conservation.
JOURNAL OF EQUINE VETERINARY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Iara Nobrega Macedo, Lucia Cristina Pereira Arruda, Breno Barros de Santana, Thalles Cloves Maciel de Moura, Maria Madalena Pessoa Guerra, Diogo Gutemberg Bezerra, Gustavo Ferrer Carneiro, Sildivane Valcacia Silva
Summary: The study aimed to evaluate the effects of adding different concentrations of ozone to quarter horse semen submitted to cryopreservation. The results indicated that ozone addition did not have beneficial effects on the cryopreservation of equine semen at the concentrations used, and decreased important fertility parameters.
ANIMAL REPRODUCTION
(2021)
Article
Agriculture, Dairy & Animal Science
Joane Isis Travassos Vieira, Taylane Alves da Silva, Williane Maria Pereira Barbosa, Gabriela Lima de Azevedo, Lucia Cristina Pereira Arruda, Maria Madalena Pessoa Guerra, Pierre Castro Soares, Ellen Cordeiro Bento da Silva
ANIMAL REPRODUCTION
(2020)
Article
Agriculture, Dairy & Animal Science
Robespierre Augusto Joaquim Araujo Silva, Andre Mariano Batista, Lucia Cristina Pereira Arruda, Helder Melo de Souza, Igor Henrique de Azevedo Valenca Nery, Wilton Arruda Gomes, Pierre de Castro Soares, Sildivane Valcacia Silva, Maria Madalena Pessoa Guerra
ANIMAL REPRODUCTION
(2019)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.
