Article
Reproductive Biology
Marcella Spinaci, Chiara Nerozzi, Beatrice Mislei, Olga Blanco-Prieto, Gaetano Mari, Giovanna Galeati, Diego Bucci
Summary: This study investigated the impact of glyphosate and glyphosate-based herbicides on stallion sperm. The results indicate that the commercial formulation Roundup has a more toxic effect on sperm motility and viability compared to the active molecule glyphosate. The negative impact is likely due to damage to the sperm membrane and mitochondria, as well as redox imbalance. Interestingly, horse sperm appear to be more sensitive to higher concentrations of Roundup compared to other species such as humans and pigs.
Article
Reproductive Biology
Camilo Hernandez-Aviles, Luisa Ramirez-Agamez, Dickson D. Varner, Charles C. Love
Summary: The process of fertilization involves acrosomal exocytosis (AE) as the interaction between sperm and oocyte. While the role of sperm acrosome in fertilization has been extensively studied, there is limited knowledge about the molecular mechanisms governing acrosomal function in non-mice or human species. Evaluating sperm acrosomal function should be a routine test for individuals selected for breeding potential, even though acrosomal dysfunction-related subfertility is less common in large animals. High throughput technologies such as flow cytometry and mass spectrometry-based proteomic analysis can be used to study acrosomal function and discover new markers of acrosomal dysfunction in stallions.
Article
Biophysics
Francisca A. Ebel, Ana C. Liaudat, Damian A. Blois, Virginia Capella, Martin F. Broglia, Cesar A. Barbero, Nancy Rodriguez, Pablo Bosch, Claudia R. Rivarola
Summary: This study proposes novel soft materials based on hydrogel for enhancing the selection of high-quality stallion sperm. The hydrogel surfaces, characterized by their interfacial properties and modified through copolymerization with neutral and ionic co-monomers, exhibit favorable outcomes in terms of sperm adhesion capacity and metabolic activity. The results suggest that these hydrogel surfaces have potential applications in selecting high-quality stallion sperm and studying other mammalian reproductive cells.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Reproductive Biology
Camilo Hernandez-Aviles, Luisa Ramirez-Agamez, Dickson D. Varner, Charles C. Love
Summary: This study developed an assay to evaluate acrosome function in stallion sperm and validated its relationship with stallion fertility. The assay demonstrated that incubation of stallion sperm in a lactate-only medium promotes acrosomal exocytosis. This method can be used to assess stallion sperm in different conditions and predict the success of artificial insemination programs.
Article
Multidisciplinary Sciences
Haruhiko Miyata, Seiya Oura, Akane Morohoshi, Keisuke Shimada, Daisuke Mashiko, Yuki Oyama, Yuki Kaneda, Takafumi Matsumura, Ferheen Abbasi, Masahito Ikawa
Summary: SPATA33 interacts with sperm calcineurin via a PQIIIT sequence, and its knockout mice exhibit reduced sperm motility and impaired male fertility, which phenocopies Ppp3cc and Ppp3r2 knockout mice. Further analysis reveals that SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Obstetrics & Gynecology
Elisabetta Baldi, Meurig T. Gallagher, Stepan Krasnyak, Jackson Kirkman-Brown
Summary: The sixth edition of the World Health Organization manual explores extended examination methods that offer key diagnostics for investigating male reproductive system function. These methods go beyond basic semen analysis and can specifically guide the clinical characterization of fertile or infertile men. Multiparametric scoring for sperm morphological defects, sperm DNA fragmentation, and computer-assisted analysis of sperm or semen are among the most widely used and debated techniques.
FERTILITY AND STERILITY
(2022)
Article
Agriculture, Dairy & Animal Science
Won-Ki Pang, Ji-Hyun Son, Do-Yeal Ryu, Md Saidur Rahman, Yoo-Jin Park, Myung-Geol Pang
Summary: This study investigated the effects of differential mRNA expression of selected genes on fertilisation parameters in a porcine model. The results revealed a significant correlation between HSPD1 expression and male fertility, suggesting HSPD1 as a potential biomarker for superior boar selection in artificial insemination, ultimately boosting field fertility.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2022)
Article
Cell Biology
Joao C. Ribeiro, Raquel L. L. Bernardino, Ana Goncalves, Alberto Barros, Giuseppe Calamita, Marco G. G. Alves, Pedro F. F. Oliveira
Summary: Osmoregulation is essential for sperm function, and aquaglyceroporins play a crucial role in glycerol permeability, which is important for sperm motility. AQP7 is especially significant in glycerol transport, and its impairment may lead to reduced sperm motility in asthenozoospermic individuals.
Article
Reproductive Biology
Suzane Peres Campanholi, Sebastiao Garcia Neto, Andrea Cristina Basso, Joao Diego de Agostini Losano, Adriano Felipe Perez Siqueira, Marcilio Nichi, Mayra Elena Ortiz D'Avila Assumpcao, Luara Afonso de Freitas, Claudia Cristina Paro de Paz, Antonio Sergio Ferraudo, Fabio Morato Monteiro, Lindsay Unno Gimenes
Summary: In this study, three subpopulations of sperm were identified in cryopreserved Senepol semen, but only two subpopulations (SBP1 and SBP2) were found to be associated with IVEP. These findings suggest that the model based on SBP1 and SBP2 may help estimate embryo production in Senepol bulls more accurately.
Article
Cell Biology
Kristin A. Hook, Lauren M. Wilke, Heidi S. Fisher
Summary: Research shows that the width of the sperm hook in rodents is negatively correlated with sperm aggregation and swimming speed, indicating that larger hooks may hinder sperm movement within this group.
Review
Andrology
Kinga Domrazek, Ilona Kaszak, Szymon Kanafa, Mariusz Sacharczuk, Piotr Jurka
Summary: Mycoplasma spp. are difficult to detect bacteria, and PCR is the most effective diagnostic tool for detecting them. However, there is a lack of information about the pathogenicity and prevalence of different species. The impact of Mycoplasma on human and canine fertility is still controversial, but it is more common in dogs. Canine and human Mycoplasma are phylogenetically related and some species cluster together.
ASIAN JOURNAL OF ANDROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Shuai Wang, Hongjie Qiao, Pengxiang Wang, Yuan Wang, Danian Qin
Summary: Zdhhc19 knockout mice did not show disruption in spermatogenesis but exhibited male infertility with defects in sperm morphology and functions, highlighting the essential role of Zdhhc19 in sperm production and fertility in mice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Andrology
Yoo-Jin Park, Byeong-Mu Lee, Won-Ki Pang, Do-Yeal Ryu, Md Saidur Rahman, Myung-Geol Pang
Summary: This study investigated the relationship between sequential changes in protein expression during epididymal sperm maturation and sperm motility and fertility in bovine. The study found that irregular modifications of mitochondrial proteins during epididymal sperm maturation may lead to excessive ROS production and premature activation of spermatozoa, resulting in a decline in sperm motility and bull fertility.
WORLD JOURNAL OF MENS HEALTH
(2022)
Review
Agriculture, Dairy & Animal Science
Gerhard van der Horst
Summary: Quantitative evaluation of sperm quality using modern technologies such as computer-aided sperm analysis is crucial in understanding sperm functionality and assisting in the propagation of wildlife species.
Article
Reproductive Biology
Camilo Hernandez-Aviles, Luisa Ramirez-Agamez, Mariah Pearson, Anne M. N. Beckham, Dickson D. Varner, Charles C. Love
Summary: Analysis of sperm morphology is essential in assessing stallion fertility. DIC and PH methods show higher agreement in assessing normal sperm. However, other methods show varying agreement with DIC depending on sperm quality. Underestimation of abnormalities in staining methods may impact clinical interpretation of stallion fertility.
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.