Article
Agriculture, Dairy & Animal Science
Brenda Florencia Gimeno, Maria Victoria Bariani, Lucia Laiz-Quiroga, Eduardo Martinez-Leon, Micaela Von-Meyeren, Osvaldo Rey, Adrian Angel Mutto, Claudia Elena Osycka-Salut
Summary: This study improved the yield of fertilization-capable sperm from equine frozen sperm by optimizing manipulation conditions and establishing a primary culture model of oviductal cells. The in vitro model demonstrated the potential fertilizing capacity of sperm released from oviductal cells, suggesting advancements in handling and selection of cryopreserved sperm for improved efficiencies in assisted reproductive techniques.
Article
Reproductive Biology
E. M. Donnellan, P. Lonergan, K. G. Meade, S. Fair
Summary: Despite passing quality control checks, bulls with apparently normal semen quality may have low field fertility rates. This study assessed the interaction between spermatozoa and the female reproductive tract in bulls with divergent field fertility. The results showed that there were no significant differences in most sperm motility and functional parameters between high and low fertility bulls. However, high fertility bulls had a higher straight-line velocity and a stronger binding ability to oviductal explants. There was also a positive correlation between straight-line velocity and sperm rheotactic response.
Article
Genetics & Heredity
Julie M. Cridland, David J. Begun
Summary: In species with internal fertilization, sperm and seminal fluid are transferred from male to female during mating. The role of most molecules in fertilization coordination or other functions is not well understood. In Drosophila, exosomes from the accessory gland are transferred to females, but their cargoes have not been described. The study finds that mated females carry male-derived transcripts from many genes, including those that are markers of the accessory gland and known seminal fluid proteins. The observations also support the isolation of intact sperm transcripts from female sperm storage organs.
G3-GENES GENOMES GENETICS
(2023)
Article
Multidisciplinary Sciences
Ayako Gotoh, Mika Takeshima, Ken-ichi Mizutani
Summary: After copulation, insect females store sperm in a spermatheca for some duration until fertilization. This study found that the nearly anoxic environment in the spermatheca induces sperm immobilization, possibly due to a shortage of ATP generated from only glycolysis under near-anoxic conditions. Artificial anoxic conditions were shown to sustain viable sperm cells, indicating the importance of near-anoxia for long-term sperm storage in ant queens.
SCIENTIFIC REPORTS
(2023)
Article
Veterinary Sciences
Essam A. A. Almadaly, Abdel-Basaer S. Abdel-Salam, Ferial M. M. Sahwan, Khaled A. A. Kahilo, Tarek K. K. Abouzed, Wael B. B. El-Domany
Summary: This study aimed to identify components in seminal plasma (SP) and/or serum that are closely associated with the in vivo fertility of buffalo bulls. Fourteen healthy mature buffalo bulls were divided into fertile and subfertile groups based on their in vivo fertility. Semen and serum samples were collected, and various analyses were performed, including sperm characteristics, hormonal and biochemical measurements, and proteomic analysis. Significant differences were observed in sperm characteristics, hormonal levels, and biochemical components between the fertile and subfertile groups. Protein bands of specific sizes were found to be associated with fertility in buffalo bull SP. Overall, evaluating sperm characteristics, hormonal and biochemical components can be reliable predictors of buffalo bull fertility, and certain protein bands may serve as markers for fertility in buffalo bull SP.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Veterinary Sciences
Ali Jawad, Dongjin Oh, Hyerin Choi, Mirae Kim, Lian Cai, Joohyeong Lee, Sang-Hwan Hyun
Summary: This study aimed to investigate the protective effect of Myo-Ins on boar sperm against oxidative stress during liquid preservation and determine the optimal concentration of Myo-Ins. The results showed that different concentrations of Myo-Ins had different effects on the quality of boar sperm. The addition of 2 mg/mL Myo-Ins improved sperm motility, viability, membrane integrity, mitochondrial membrane potential, and effective survival time, as well as increasing the expression of antioxidative genes.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Review
Medicine, General & Internal
Michal Jeseta, Anna Pospisilova, Lenka Mekinova, Katerina Franzova, Pavel Ventruba, Eva Lousova, Bartosz Kempisty, Tomas Ozdian, Jana Zakova, Igor Crha
Summary: The compounds in seminal plasma have potential as biomarkers for male fertility and can be used for diagnosing different types of azoospermia. Distinguishing between obstructive and non-obstructive azoospermia is important for effective treatment. Proteins in seminal plasma, particularly testicular and epididymal proteins, can serve as biomarkers for diagnosing azoospermia. Combining multiple biomarkers, such as TEX101 and ECM1 or TEX101 and SPAG1, can improve diagnostic accuracy.
Article
Agriculture, Dairy & Animal Science
Elba Rodriguez-Pena, Diego Suarez, Graciela Estevez-Perez, Patricia Verisimo, Noelia Barreira, Luis Fernandez, Ana Gonzalez-Tizon, Andres Martinez-Lage
Summary: Female spider crabs can store sperm for several years, but the viability and DNA integrity of the stored sperm decline sharply during the first few months.
Article
Developmental Biology
Haitao Nie, Yue Wen, Ruidong Zhang, Mengjuan Wu, Yuqian Zhang, Yunlu Xu, Jixiang Zhan, Xiaobing Wu
Summary: This study investigated the morphological variations related to sperm storage and gene expression patterns in female Chinese alligators, highlighting the seasonal environmental influences on sperm storage mechanisms. Through RNA sequencing analysis, genes involved in PPARs and FOXO signaling pathways were identified, contributing to the understanding of sperm storage in A. sinensis.
REPRODUCTION FERTILITY AND DEVELOPMENT
(2022)
Article
Reproductive Biology
Pradeep Nag, Arumugam Kumaresan, Sivamanikandan Akshaya, Ayyasamy Manimaran, Duraisamy Rajendran, Nilendu Paul, Ankur Sharma, Thirumalaisamy Karuthadurai, Saraf Kaustubh, Sakthivel Jeyakumar, Kerekoppa Ramesha
Summary: This study standardized an in vitro oviduct explants model for cattle and evaluated the spermatozoa binding ability and phenotypic characteristics in breeding bulls with high and low sperm DNA fragmentation index (DFI%). Spermatozoa from bulls with low DFI% showed significantly better membrane integrity, acrosome intactness, and mitochondrial membrane potential. The oviduct binding ability of spermatozoa was found to be significantly higher in bulls with low DFI% compared to bulls with high DFI%.
Article
Reproductive Biology
Olatunji Abubakar Jimoh, Wahab Adekunle Oyeyemi, Hafsat Ololade Okin-Aminu, Bolaji Fatai Oyeyemi
Summary: This study found that mistletoe supplementation in bucks' diets significantly increased sperm concentration, progressive motility, velocity, and head amplitude compared to the control group. The inclusion of herbal supplements linearly increased seminal total antioxidant activity while decreasing seminal lipid peroxidation levels. Bucks fed on herbal supplements showed depleted gonadal and extra-gonadal sperm reserves compared to the control group.
Article
Genetics & Heredity
Kaelina D. Lombardo, Hayley K. Sheehy, Julie M. Cridland, David J. Begun
Summary: Most eukaryotic genes are vertically transmitted from ancestors, but gene gain and loss can also occur, resulting in variable gene numbers across species. Previous studies in Drosophila have shown that de novo genes, which are born from non-genic ancestral sequences, are commonly expressed in male reproductive tissues. However, there is a lack of research on female reproductive tissues. In this study, we analyzed the transcriptomes of three female reproductive tract organs in three Drosophila species to identify putative D. melanogaster-specific de novo genes.
G3-GENES GENOMES GENETICS
(2023)
Article
Anatomy & Morphology
Maria Alice Garcia Bento, Laura S. Lopez Greco, Fernando L. Mantelatto, Fernando Jose Zara
Summary: This study describes the mating behavior of Hypoconcha parasitica under laboratory conditions, focusing on the spermathecal morphology and the storage and release of spermatozoa. The study found no mate guarding behavior in H. parasitica, and sperm transfer occurred with each pair maintaining the protection shield on their dorsum. The spermathecal organization suggests that sperm release during fertilization is achieved through muscular action exerted by the female. Additionally, the first pleopod in H. parasitica seems to be involved in the transfer of spermatozoa and oocytes, forming a temporary chamber for fertilization.
ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY
(2022)
Article
Cell Biology
Sergio Garcia-Segura, Javier del Rey, Laia Closa, Iris Garcia-Martinez, Carlos Hobeich, Ana Belen Castel, Francisco Vidal, Jordi Benet, Jordi Ribas-Maynou, Maria Oliver-Bonet
Summary: The development of new biomarkers for male infertility is crucial. This study found a relationship between seminal microbiota and sperm quality parameters, chromatin integrity, and oxidative stress. Specific genera were associated with different sperm quality defects, suggesting the potential use of microbial profiles as biomarkers for male fertility.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Yentel Mateo-Otero, Pol Fernandez-Lopez, Jordi Ribas-Maynou, Jordi Roca, Jordi Miro, Marc Yeste, Isabel Barranco
Summary: Metabolomic approaches were used to analyze pig seminal plasma and identify specific relationships between metabolites and sperm quality and functionality. The study found potential metabolite patterns for specific sperm parameters, indicating the possibility of using these metabolites as biomarkers for sperm function.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.