Article
Entomology
Nan Xu, Sheng-Fei Wei, Hai-Jun Xu
Summary: The brown planthopper Nilaparvata lugens can develop into short-winged or long-winged adults based on environmental conditions, with the transcription factor NlFoxO playing a key role in directing wing morph development. RNA sequencing analysis revealed that NlFoxO regulates wing bud development by mainly controlling the expression of cell proliferation-associated genes.
Article
Entomology
Kaikai Mao, Zhijie Ren, Wenhao Li, Tingwei Cai, Xueying Qin, Hu Wan, Byung Rae Jin, Shun He, Jianhong Li
Summary: Carboxylesterases (CarEs) are an important detoxification enzyme family in insecticide resistance, with specific genes in the insect Nilaparvata lugens being identified and shown to be induced by various insecticides. Tissue-specific expression analysis showed certain CarE genes are highly expressed in the midgut and fat body, while others are more highly expressed in the head. The NlCarE1 gene was identified as a main candidate gene for nitenpyram resistance, with evidence suggesting a cooperative relationship with NlCarE19 in resistance formation. These findings provide a foundation for further understanding of insecticide resistance mediated by CarE in N. lugens.
Article
Agriculture, Multidisciplinary
Jinghua Zhu, Zengxin Li, Meng Zhang, Weiwei Qin, Yoonseong Park, Yueping He
Summary: This study utilized RNA-seq to identify a set of transporter and receptor genes enriched in the excretory organs of the brown planthopper (BPH). RNA interference assays showed that the knockdown of certain genes significantly affected the survival and honeydew secretion of BPH. These genes may serve as potential targets for insect control.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Environmental Sciences
Yibei Cheng, Yimin Li, Wenru Li, Yuanyuan Song, Rensen Zeng, Kai Lu
Summary: The study demonstrates that inhibition of hepatocyte nuclear factor 4 confers imidacloprid resistance in brown planthopper by regulating cytochrome P450 and UDP-glycosyltransferase genes. Knockdown of HNF4 enhances resistance to imidacloprid in BPH, leading to up-regulation of cytochrome P450- and UGT-mediated metabolic detoxification pathways. Over-expression of UGT-1-7, UGT-2B10, and CYP6ER1 in the resistant strain indicates potential targets for pest management.
Article
Genetics & Heredity
Wen-Hua Xue, Nan Xu, Sun-Jie Chen, Xin-Yang Liu, Jin-Li Zhang, Hai-Jun Xu
Summary: In insects, the second insulin receptor (InR2) has distinct functions from the InR homologue (NlInR1) in terms of fuel metabolism, lifespan, and starvation tolerance, while resembling NlInR1 in nymph development and reproduction. Proper expression of NlInR2 is crucial for maintaining symmetric vein patterning in wings.
Article
Multidisciplinary Sciences
Cai-Di Xu, Yong-Kang Liu, Ling-Yu Qiu, Sha-Sha Wang, Bi-Ying Pan, Yan Li, Shi-Gui Wang, Bin Tang
Summary: GFAT and PFK play a role in regulating chitin metabolism by affecting the expression of related genes, leading to severe malformation and mortality rates in Nilaparvata lugens. These findings may aid in the development of new chitin synthesis inhibitors for pest control.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Litong Sun, Jitong Li, Yongyan Liu, Ali Noman, Lin Chen, Jinglan Liu
Summary: This study found that overexpression of OsNCED3 gene in rice can improve resistance to the brown planthopper (BPH) and has potential application value in rice breeding programs.
Article
Biotechnology & Applied Microbiology
Xifa Yang, Shaokai Liu, Wenhui Lu, Mengfang Du, Zhuangzhuang Qiao, Zhen Liang, Yiting An, Jing Gao, Xiang Li
Summary: This study evaluated the lethal and teratogenic effects of the Notch signaling pathway ligand genes Nldl and Nljag on the brown planthopper (BPH) population. The results confirmed the potential of Nldl and Nljag as target genes for RNAi biopesticides and proposed optimized dosages for BPH control.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Entomology
Jia-Bao Lu, Peng-Peng Ren, Qiao Li, Fang He, Zhong-Tian Xu, Sai-Nan Wang, Jian-Ping Chen, Jun-Min Li, Chuan-Xi Zhang
Summary: This study identified 10 ApoD-like genes in Nilaparvata lugens (BPH) and found that NlApoD2 is essential for BPH development and survival. NlApoD4/5 are highly expressed in testes and may play a role in reproduction. Additionally, NlApoD3-5/9, NlApoD3-5, and NlApoD9 were up-regulated after treatment with lipopolysaccharide, H2O2, and ultraviolet-C, respectively, suggesting their potential roles in stress resistance.
Article
Physiology
Hui-Ru Si, Si-Si Sun, Yong-Kang Liu, Ling-Yu Qiu, Bin Tang, Fang Liu, Qiang Fu, Cai-Di Xu, Pin-Jun Wan
Summary: In this study, the knockdown of NlGFAT or NlPFK in brown planthoppers resulted in metabolism disorders, with significant changes in trehalose, glucose, and glycogen metabolism pathways. Knockdown of NlGFAT led to increased trehalose and glycogen content, while knockdown of NlPFK caused extreme increases in glucose and glycogen content. The knockdown of NlGFAT or NlPFK also affected the glycolytic pathway and pyruvate kinase activity, as well as ATP content.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Priyadarshini Rout, Nihal Ravindranath, Dinkar Gaikwad, Satyabrata Nanda
Summary: This study analyzed the transcriptomic, physiological, and gene-silencing responses of the BPH biotype 4 during compatible and incompatible rice-BPH interactions. It was found that in the incompatible interaction, there was a significant reduction in honeydew production and negative weight gain in BPH. Additionally, the trehalose and glucose contents were significantly high and low, respectively, during the incompatible interaction. The study also identified differentially expressive genes involved in vital BPH physiological processes.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Agronomy
Bin Zeng, Ya-Ting Liu, Ze-Rui Feng, Fu-Rong Chen, Shun-Fan Wu, Chris Bass, Cong-Fen Gao
Summary: The overexpression of genes CYP6ER1vA and CYP439A1 contributes to buprofezin resistance in brown planthopper (BPH), and this resistance is associated with low-level resistance to other insecticides.
PEST MANAGEMENT SCIENCE
(2023)
Article
Agronomy
Chao Ma, Wei Gui, Yuanyuan Zhang, Amir Zaman Shah, Gang Xu, Guoqing Yang
Summary: Seed priming with decoyinine (DCY) enhances the resistance of rice to the brown planthopper (BPH) by increasing the activity of defense-related enzymes and concentrations of certain compounds, while decreasing the concentration of malondialdehyde (MDA). Transcriptome analysis further reveals the differential expression of genes related to defense response, transcription factors, secondary metabolites, reactive oxygen species, and cell wall organization. It suggests that DCY may enhance rice resistance to BPH by regulating WRKY transcription factor genes.
Article
Entomology
Chao Zhang, Meng-Sha Mao, Xiang-Dong Liu
Summary: This study investigated the wing dimorphism of the brown planthopper and found that the wing morphs were primarily determined by genetics and not influenced by environmental factors. Additionally, the expression patterns of wing development-regulated genes differed between the different wing morphs.
Article
Genetics & Heredity
Xuan Chen, Ze-Dong Li, Dan-Ting Li, Ming-Xing Jiang, Chuan-Xi Zhang
Summary: This study systematically identified and characterized HSP70 and DNAJ family members in the brown planthopper, highlighting their high diversity. The research showed that these genes are highly expressed in the adult stage and gonads, and play essential roles in nymphal development, oogenesis, and female fertility. Additionally, one HSP70 gene was found to be crucial for thermal tolerance of eggs, shedding light on the biological roles of HSP70/DNAJ in insects.