Article
Agronomy
Shang Gao, Yunfeng Jiang, Hong Zhou, Yaxi Liu, Huihui Li, Chunji Liu, Zhi Zheng
Summary: This study validated and mapped a Fusarium crown rot resistant locus on chromosome arm 6HL in barley using near isogenic lines and transcriptome sequences. The locus was confirmed to have a large effect, and it was fine-mapped to a 0.9 cM interval covering a physical distance of approximately 547 kb. Six co-segregating markers and candidate genes associated with the resistance were identified. These findings will improve the efficiency of incorporating the targeted locus into barley breeding programs and facilitate the cloning of causal genes responsible for the resistance.
THEORETICAL AND APPLIED GENETICS
(2023)
Article
Plant Sciences
Jacob R. MacWilliams, Paul D. Nabity, Kerry E. Mauck, Isgouhi Kaloshian
Summary: This study investigates the interaction between cowpea plants and aphids and identifies the genes underlying cowpea resistance to aphids. The results show that the feeding behavior of aphids remodels the transcriptome of cowpea and that this remodeling depends on both the duration of feeding and host-plant resistance. The study also identifies candidate genes involved in aphid resistance, including both conventional resistance genes and novel genes.
Article
Plant Sciences
Sina Nouraei, Md Sultan Mia, Hui Liu, Neil C. Turner, Guijun Yan
Summary: This study analyzed gene expression profiling in wheat to investigate the genetic control of drought tolerance. The researchers identified candidate genes and genetic markers associated with the qDSI.4B.1 locus, which can be used in breeding programs for drought tolerance. The study also revealed the biological processes involved in the response to drought stress.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Judit Kolozsvarine Nagy, Ildiko Schwarczinger, Lorant Kiraly, Renata Bacso, Attila L. Adam, Andras Kunstler
Summary: This study investigates the effects of short- and long-term heat stress on barley resistance to powdery mildew infection, finding that heat stress negatively affects resistance and enhances susceptibility in barley cultivars.
Article
Agronomy
Ahmed Sherif, Bo Zhang, Bi Wu, Yong Hu, Shuangle Li, Xiangchun Zhou, Ali Mahmoud Elbadri, Mostafa Elshenawy, Mahmoud El. M. El-Badawy, Ibrahim O. Hassan, Sedhom A. Sedhom, Mahmoud Abo-Yousef, Mohammed Ayaad, Yongzhong Xing
Summary: A rapid QTL cloning strategy, Pseudo-NIF2, is proposed to save time compared to conventional methods, by generating a population that segregates at the target QTL but is fixed at other QTLs.
MOLECULAR BREEDING
(2023)
Article
Biochemistry & Molecular Biology
Tanushree Halder, Hui Liu, Yinglong Chen, Guijun Yan, Kadambot H. M. Siddique
Summary: This study phenotyped root traits in wheat using 10 pairs of near-isogenic lines targeting four genomic regions (GRs) and identified 15 putative candidate genes associated with different root traits. Among the candidate genes, UDP-glycosyltransferase (UGT)-encoding genes and leucine-rich repeat receptor-like protein kinase (LRR-RLK)-encoding gene showed important functions for root trait control in other crops. Further confirmation and validation of these candidate genes are needed for marker-assisted wheat breeding.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agronomy
Yunxiao Zhang, Hui Liu, Guijun Yan
Summary: The study assessed seven pairs of NILs targeting four QTL on wheat chromosome arms and found significant differences in PH and other traits among NIL pairs. SNP and candidate gene information can be useful for marker-assisted selection in breeding programs, and the four targeted QTL are crucial genomic regions controlling agronomic traits.
MOLECULAR BREEDING
(2021)
Article
Plant Sciences
Shibani Mohapatra, Alok Kumar Panda, Akshaya Kumar Bastia, Arup Kumar Mukherjee, Priyadarshini Sanghamitra, Jitendriya Meher, Shakti Prakash Mohanty, Sharat Kumar Pradhan
Summary: By using marker-assisted backcross breeding, advantageous genes were introduced into the Swarna variety, resulting in progenies with higher resistance to bacterial blight and submergence tolerance. Through multiple generations of backcrossing, superior hybrid lines with higher yield and multiple desirable genes were developed, exhibiting enhanced disease resistance and submergence tolerance.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Yia Yang, Thang C. La, Jason D. Gillman, Zhen Lyu, Trupti Joshi, Mariola Usovsky, Qijian Song, Andrew Scaboo
Summary: This study aimed to explore the genetic effects of introducing wild soybean genes on soybean protein and oil. Multiple quantitative trait loci were identified, and a new detection method was successfully developed to introduce beneficial traits from wild soybeans into modern soybean cultivars.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Yanming Zhao, Xiaojie Ma, Miaomiao Zhou, Junyan Wang, Guiying Wang, Chengfu Su
Summary: This study validated a major quantitative trait locus (QTL), qKW-1, for kernel width in maize and predicted candidate genes. Near-isogenic lines (NILs) were developed through continuous backcrossing and marker-assisted selection. The qKW-1 locus was mapped between specific intervals, and two candidate genes, GRMZM2G083176 and GRMZM2G081719, were identified. Reverse transcription-polymerase chain reaction (RT-qPCR) validation further supported the association of these candidate genes with qKW-1.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Krzysztof Mikolajczak, Anetta Kuczynska, Piotr Ogrodowicz, Agnieszka Kielbowicz-Matuk, Hanna Cwiek-Kupczynska, Agata Daszkowska-Golec, Iwona Szarejko, Maria Surma, Pawel Krajewski
Summary: High temperature shock affects plant growth and productivity. This study found that gibberellin-deficient barley mutants showed modifications in plant stature and crown-specific transcriptome remodeling under increased temperature.
Article
Plant Sciences
Anne Quillevere-Hamard, Gwenola Le Roy, Angelique Lesne, Christophe Le May, Marie-Laure Pilet-Nayel
Summary: The study evaluated the aggressiveness of diverse French isolates of Aphanomyces euteiches on Near Isogenic Lines (NILs) carrying different resistance QTLs. The isolates were categorized into three groups based on aggressiveness levels, while the pea lines were classified into three groups based on their resistance levels to the isolates carrying different resistance QTLs.
Article
Food Science & Technology
Antoni Femenias, Enric Llorens-Serentill, Antonio J. Ramos, Vicente Sanchis, Sonia Marin
Summary: This study focuses on the application of Near-Infrared Hyperspectral Imaging (HSI-NIR) for detecting and discriminating DON in Fusarium Damaged Kernels (FDK) exceeding the EU limit. The results show that HSI-NIR can accurately predict DON levels and effectively classify wheat contaminations.
Article
Agronomy
Zhitong Chen, Zhi Zheng, Wei Luo, Hong Zhou, Zhaoyang Ying, Chunji Liu
Summary: Trichomes in Arabidopsis were studied to identify a major locus on chromosome responsible for trichome characteristics. Near isogenic lines derived from this locus showed similar traits, indicating its importance. These NILs with homogeneous genetic backgrounds could be valuable in studying the relationship between trichome density and resistance to stresses.
MOLECULAR BREEDING
(2021)
Article
Plant Sciences
Junling Dou, Yinping Wang, Huihui Yang, Huanhuan Niu, Dongming Liu, Sen Yang, Huayu Zhu, Shouru Sun, Luming Yang
Summary: This study successfully developed branchless near isogenic lines (NILs) in watermelon, which provide ideal materials for watermelon breeding and plant architecture research.
HORTICULTURAL PLANT JOURNAL
(2022)
Article
Agronomy
Bishal G. Tamang, Jose R. Lopez, Erik McCoy, Allison Haaning, Ahmad Sallam, Brian J. Steffenson, Gary J. Muehlbauer, Kevin P. Smith, Walid Sadok
Summary: Research suggests that winter survival in winter barley is closely associated with smaller xylem vessel diameters, a trait that is heritable and potentially identifiable through gas exchange measurements. Genotypes with smaller vasculature also tend to have lower stomatal conductance and transpiration rates, which could aid in breeding for freezing survival. These findings point to new phenotyping approaches to accelerate the breeding process.
JOURNAL OF AGRONOMY AND CROP SCIENCE
(2022)
Article
Plant Sciences
Ian G. McNish, Kevin P. Smith
Summary: This study utilized unmanned aerial vehicle technology to assess oat disease severity using spectral data and found that the accuracy of photo estimates was highest for adult plants shortly before they began to senesce. The research suggested that UAV-mounted sensors could improve disease scoring efficiency, but further investigation into spectral characteristics of disease severity at all plant growth stages is needed for accurate full-season measurements.
Article
Agronomy
Jeffrey L. Neyhart, Lucia Gutierrez, Kevin P. Smith
Summary: This study utilized barley breeding nurseries to optimize the selection of test locations based on multitrait data, leading to a significant reduction in the number of locations and phenotyping costs. The approach allowed for the selection of locations to match different sets of traits, increasing resource efficiency for regional evaluation trials.
Article
Genetics & Heredity
Lauren J. Brzozowski, Haixiao Hu, Malachy T. Campbell, Corey D. Broeckling, Melanie Caffe, Lucia Gutierrez, Kevin P. Smith, Mark E. Sorrells, Michael A. Gore, Jean-Luc Jannink
Summary: This study investigates the variation and genetic influence on the metabolite profiles of oats, specifically the seed metabolites with antioxidant properties. The research finds reduced genetic variation in modern oat germplasm compared to diverse germplasm, mainly due to increased seed size from intensive breeding. The abundance of avenanthramides, one of the specialized metabolites, increases with seed size, while avenacoside abundance decreases. The study highlights the importance of understanding how plant breeding influences plant traits and the genetic basis behind these changes.
G3-GENES GENOMES GENETICS
(2022)
Article
Agronomy
John H. Price, David L. Van Tassel, Valentin D. Picasso, Kevin P. Smith
Summary: This study conducted a multilocation trial of silflower genotypes and identified heritable traits and patterns of correlation across years and environments. The study also found several important traits contributing to yield and lodging levels. These findings will guide future silflower domestication efforts and breeding programs.
Article
Ecology
John H. Price, Andrew R. Raduski, Yaniv Brandvain, David L. Van Tassel, Kevin P. Smith
Summary: In this study, we successfully identified the self-incompatibility locus in Silphium integrifolium and developed a fast and efficient method for its determination, which could be applied to other species.
Article
Plant Sciences
Lauren J. Brzozowski, Malachy T. Campbell, Haixiao Hu, Melanie Caffe, Lucia Gutierrez, Kevin P. Smith, Mark E. Sorrells, Michael A. Gore, Jean-Luc Jannink
Summary: In this study, we tested the applicability of genomic prediction methods based on biochemical pathways for oat seed metabolites. The results showed that metabolite kernels developed from the discovery panel significantly improved the prediction accuracy of the same metabolites in the validation panel. However, this method did not improve the prediction accuracy for GC-MS metabolites.
Article
Agronomy
Jeffrey L. Neyhart, Kevin A. T. Silverstein, Kevin P. Smith
Summary: Extending the genomewide prediction framework to include environmental covariates can improve the accuracy of predicting the performance of new plant genotypes under new environmental conditions. However, the similarity between training and target environments is a limiting factor for accurate predictions.
Article
Plant Sciences
Yun-Yu Chen, Miriam Schreiber, Micha M. Bayer, Ian K. Dawson, Peter E. Hedley, Li Lei, Alina Akhunova, Chaochih Liu, Kevin P. Smith, Justin C. Fay, Gary J. Muehlbauer, Brian J. Steffenson, Peter L. Morrell, Robbie Waugh, Joanne R. Russell
Summary: The pericentromeric regions of barley show reduced diversity compared to wild barley, and there are different types of variations and linkage disequilibrium between the domesticated and wild gene pools. The chromosome 7H clearly differentiates barley types associated with different geographical origins.
Article
Agronomy
Jose R. Lopez, Bishal G. Tamang, Daniel M. Monnens, Kevin P. Smith, Walid Sadok
Summary: This study aims to identify locally-adapted high temperature stress-tolerant oat breeding lines and propose a screening method. Canopy cooling was found to be the main factor explaining the superior yield performance of heat-stress tolerant lines.
EUROPEAN JOURNAL OF AGRONOMY
(2022)
Article
Agronomy
Walid Sadok, Jochum J. Wiersma, Brian J. Steffenson, Sigelinde S. Snapp, Kevin P. Smith
Summary: Continental cropping systems are facing extreme temperature trends, which pose significant challenges to the growth of winter barley. This study proposes a framework to identify traits that enable adaptation to stressful conditions, while minimizing costs. The authors discuss the feasibility of implementing this framework and emphasize the importance of identifying relevant genetic resources and physiological traits.
FIELD CROPS RESEARCH
(2022)
Article
Agronomy
Chris Massman, Brigid Meints, Javier Hernandez, Karl Kunze, Kevin P. Smith, Mark E. Sorrells, Patrick M. Hayes, Lucia Gutierrez
Summary: This study aimed to identify quantitative trait loci associated with threshability and evaluate their utility in genomic prediction models. Two loci on chromosomes 2H and 3H were found to be associated with threshability, but their impact on marker-assisted selection may be limited. The incorporation of these loci as covariates in the model did not improve predictive ability, while a multi-trait model including grain test weight showed improved predictive ability. Overall, genomic selection has a high predictive ability for threshability in naked barley.
Article
Multidisciplinary Sciences
Tarun Gangwar, Alexander Q. Q. Susko, Svetlana Baranova, Michele Guala, Kevin P. P. Smith, D. Jo Heuschele
Summary: Lodging poses challenges to the successful cultivation of cereal crops. A unique collaboration among disciplines of plant science, modelling and simulations, and experimental fluid dynamics was conducted to breed lodging resilient wheat and oat. Wind tunnel experiments were run to measure the stem bending behavior of both cereals and a finite-element simulation framework was created to predict their mechanical response. This framework revealed the multi-scale origin of the observed differences in stem strength of both cereals.
ROYAL SOCIETY OPEN SCIENCE
(2023)
Article
Agronomy
Ahmad H. Sallam, Matthew Haas, Yadong Huang, Zenith Tandukar, Gary Muehlbauer, Kevin P. Smith, Brian J. Steffenson
Summary: Fusarium head blight is a devastating disease of barley that reduces yield and quality, and mycotoxins produced by Fusarium species can contaminate grain. Few good sources of resistance have been identified, but mapping studies have revealed multiple QTL for FHB resistance and DON accumulation across the barley genome. Only a few QTL were considered priority targets for marker-assisted selection.
Article
Plant Sciences
Lauren J. J. Brzozowski, Malachy T. T. Campbell, Haixiao Hu, Linxing Yao, Melanie Caffe, Lucia Gutierrez, Kevin P. P. Smith, Mark E. E. Sorrells, Michael A. A. Gore, Jean-Luc Jannink
Summary: This study examines genomic prediction methods for predicting seed fatty acid content traits in oat, and finds that using related germplasm panels as training populations can improve prediction accuracy.