Article
Environmental Sciences
Sen Yang, Junhong Wu, Heli Wang, Qian Yang, Huanheng Zhang, Lihua Yang, Dan Li, Yirong Deng, Yin Zhong, Ping'an Peng
Summary: This study investigated the microbial-mediated dechlorination of chlorinated organophosphate esters (Cl-OPEs) at end-of-life vehicles dismantling sites. The dechlorination products, pathways, and mechanisms of tris(2-chloroethyl) phosphate (TCEP) were analyzed, and the important roles of Dehalococcoides and sulfate-reducing bacteria in the dechlorination process were identified.
ENVIRONMENTAL POLLUTION
(2023)
Article
Engineering, Chemical
Edoardo Dell'Armi, Marco Zeppilli, Bruna Matturro, Simona Rossetti, Marco Petrangeli Papini, Mauro Majone
Summary: The study introduces a sequential bioelectrochemical process composed of two membrane-less microbial electrolysis cells operated with synthetic groundwater, leading to a temporary reduction in PCE removal efficiency due to the establishment of sulphate and nitrate reduction processes.
Article
Environmental Sciences
Zheng-Tao Li, Si-Ying Yang, He -Ping Zhao
Summary: Inorganic arsenic and organochlorines often coexist in anoxic groundwater environments, posing challenges for bioremediation. This study investigated the dechlorination performance of a microbial consortium under different concentrations of arsenate or arsenite and found that the impact of arsenic on dechlorination differed between the two scenarios. The study also revealed that the vinyl chloride-to-ethene step was more susceptible to arsenic exposure and fermentative bacteria showed high potential for arsenic resistance. The findings provide insights for modifying bioremediation strategies at co-contaminated sites.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Engineering, Environmental
Fang Zhang, Runlei Ge, Ziren Wan, Guanghe Li, Lifeng Cao
Summary: PFASs and chlorinated solvents are common co-contaminants in soil and groundwater at firefighter training areas (FTAs). High levels of PFOA or PFOS inhibit the dechlorination of TCE by non-Dhc OHRB, while low levels enhance the dechlorination. PFOS at high concentrations kills certain bacteria and decreases biodiversity of the bacterial community.
Article
Environmental Sciences
Wei-Yu Chen, Jer-Horng Wu
Summary: This study evaluated the effects of different substrates on the reductive dechlorination of trichloroethene at contaminated sites, finding that the molasses-fed reactor exhibited superior performance. The results suggest a significant impact of the substrate on the growth of organohalide-respiring bacteria and the dechlorination process of trichloroethene.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2022)
Article
Microbiology
Maria L. Di Franca, Bruna Matturro, Simona Crognale, Marco Zeppilli, Edoardo Dell'Armi, Mauro Majone, Marco Petrangeli Papini, Simona Rossetti
Summary: This study describes the microbiome composition of a novel bioelectrochemical system (BES) for removing perchloroethylene using sequential reductive/oxidative dechlorination. The feeding composition affects the microbiome and the products of the dechlorination reaction.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Engineering, Environmental
Shuting Xiong, Jinxin Zhao, Bingliang Lun, Fengmin Chen, Yanyan Gong, Fan Wu, Jinhua Wu, Li-Zhi Huang, Weizhao Yin, Dionysios D. Dionysiou
Summary: This study achieved efficient dechlorination of trichloroethene and its removal from real groundwater with a novel composite, providing evidence to reconsider ferrous hydroxide as an inexpensive and efficient reductant for soil and groundwater contaminated with chlorinated ethenes (CEs).
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Tielong Li, Jiaxin Wen, Bingjie Li, Shihu Ding, Wei Wang
Summary: The study found that tourmaline can accelerate the biological dechlorination of TCE by promoting bacterial growth and metabolism, leading to increased ethene production and significant impact on RDase gene expression.
Article
Environmental Sciences
Silviu-Laurentiu Badea, Diana-Ionela Stegarus, Violeta-Carolina Niculescu, Stanica Enache, Amalia Soare, Roxana-Elena Ionete, Didier Gori, Patrick Hohener
Summary: This study investigated the carbon isotope fractionation of alpha-HCH during dechlorination by FeS nanoparticles at different pH values. The results showed that the apparent rate constants during dehalogenation of alpha-HCH by FeS increased with pH, with complete dechlorination only occurring at pH values 9.9 and 11.8. The study suggests that abiotic isotope fractionation by FeS must be considered in anoxic sediments and aquifers contaminated with HCH isomers.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Environmental Sciences
W. H. Lin, C. C. Chien, C. W. Lu, Deyi Hou, Y. T. Sheu, S. C. Chen, C. M. Kao
Summary: Trichloroethene (TCE) dechlorination under anaerobic conditions is an effective method for cleaning up TCE-polluted groundwater. Supplementing organic substrates can enhance hydrogen production and improve the activity of dechlorinating bacteria, but the produced hydrogen may be consumed by methanogens, leading to decreased efficiency. Addition of methanogen inhibitors can effectively inhibit methanogens growth, resulting in enhanced TCE dechlorination efficiency.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Chemistry, Inorganic & Nuclear
Caroline K. Williams, Gavin A. McCarver, Amir Lashgari, Konstantinos D. Vogiatzis, Jianbing ''Jimmy'' Jiang
Summary: The study presents a molecular copper(I) complex CuT2 as an efficient catalyst for dehalogenation of organohalides in water, with possible catalytic mechanisms proposed. CuT2 catalyst showed high efficiency and activity in water, along with excellent stability, highlighting the importance of the copper center for catalytic performance compared to a copper-free triazole unit T1.
INORGANIC CHEMISTRY
(2021)
Article
Engineering, Environmental
Marco Zeppilli, Bruna Matturro, Edoardo Dell 'Armi, Lorenzo Cristiani, Marco Petrangeli Papini, Simona Rossetti, Mauro Majone
Summary: A bioelectrochemical process combining two membrane-less reactors equipped with graphite granules counter electrode was developed for perchloroethylene removal. The reductive reactor completely reduced PCE into VC and Eth, which were efficiently removed by the oxidative reactor. Different redox niches in each compartment allowed the co-existence of both reducing and oxidative reactions, enhancing the overall efficiency of PCE removal.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Environmental Sciences
Jianling Fan, Cuiying Liu, Jinjin Zheng, Yang Song
Summary: Exploring the mechanisms of accelerated degradation of Hexachlorobenzene (HCB) in soil is significant in both scientific and practical aspects. This study investigated the effects of goethite, dithionite, and their interaction on the microbial community and HCB dechlorination in a paddy soil. The results indicated that dithionite addition, either with or without goethite, promoted HCB dechlorination by increasing microbial diversity and abundance of specific bacterial genera.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Environmental Sciences
Edoardo Dell'Armi, Marta Maria Rossi, Lucia Taverna, Marco Petrangeli Papini, Marco Zeppilli
Summary: Based on the study, it can be concluded that the bioelectrochemical system using fermentable organic substrates as electron donors shows higher activity and efficiency in removing trichloroethylene pollution, compared to directly using molecular hydrogen as an electron donor. Additionally, the bioelectrochemical system achieves higher efficiency compared to the use of fermentable substrates and other chemicals.
Article
Engineering, Environmental
Edoardo Dell'Armi, Marco Zeppilli, Maria Letizia Di Franca, Bruna Matturro, Viktoria Feigl, Monika Molnar, Zsofia Berkl, Imre Nemeth, Hafsa Yaqoubi, Simona Rossetti, Marco Petrangeli Papini, Mauro Majone
Summary: In this study, a sequential reductive/oxidative bioelectrochemical process was used to remove chlorinated aliphatic hydrocarbons (CAHs) from contaminated groundwater. The sequential system consisted of two membrane-less microbial electrolysis cells (MECs) connected in series. The results showed that the sequential process effectively mineralized CAHs and reduced their ecotoxicity, as evaluated by biomarkers and ecotoxicity tests.
JOURNAL OF WATER PROCESS ENGINEERING
(2022)
Article
Biochemical Research Methods
Nane Griem-Krey, Bente Frolund, Ales Marek, Petrine Wellendorph
Summary: GHB is both an endogenously present small molecule and a clinically prescribed drug for narcolepsy. Its mechanism of action, targeting GABA(B) receptors and high-affinity binding sites, remains to be fully understood. The use of radioligands like [H-3]HOCPCA for selectively probing GHB high-affinity binding sites shows promise in drug discovery and future investigations.
JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS
(2021)
Review
Biochemical Research Methods
Veronika Prazienkova, Ales Marek, Lenka Maletinska
Summary: CART peptides are involved in food intake regulation, stress, and other physiological functions, but their receptor(s) have not been fully characterized. Previous studies have shown that CART(61-102) and its di-iodinated analogs can bind to PC12 cells and maintain biological activity.
JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS
(2021)
Article
Biochemical Research Methods
Yue Liu, Congcong Ma, Calvin J. A. Leonen, Champak Chatterjee, Gabriela Novakova, Ales Marek, Frantisek Turecek
Summary: The study of gas-phase riboguanosine radicals with a fixed charge provides insights into the mechanism of radical generation, which is crucial for exploring reactions and processes related to nucleic acids.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Biochemical Research Methods
Seiya Asai, Lenka Zakova, Irena Selicharova, Ales Marek, Jiri Jiracek
Summary: The study utilized an IR binding assay to determine insulin levels, showing comparable results to RIA kits. Arginine stimulated glucose-induced insulin secretion in MIN6 cells, while dopamine had inhibitory effects. The effects of serotonin varied with concentration levels. The IR binding assay allows for higher throughput and broader range of measurements compared to RIA kits.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Michal Kriegelstein, Milos Hroch, Ales Marek
Summary: A convenient and straightforward method for synthesizing ibrutinib labeled with carbon-13 isotope was reported, achieving a sufficient isolated yield of [C-13(6)]-ibrutinib in the final step of the reaction sequence. The yield was calculated based on commercially available [C-13(6)]-bromobenzene.
JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS
(2021)
Article
Chemistry, Medicinal
Marketa Pimkova Polidarova, Petra Brehova, Martin Maxmilian Kaiser, Miroslav Smola, Martin Dracinsky, Joshua Smith, Ales Marek, Milan Dejmek, Michal Sala, Ondrej Gutten, Lubomir Rulisek, Barbora Novotna, Andrea Brazdova, Zlatko Janeba, Radim Nencka, Evzen Boura, Ondrej Pav, Gabriel Birkus
Summary: In this study, the activity of 3',3'-c-di(2'F,2'dAMP) and its analogues against different STING allelic forms was evaluated. The synthesis of acyloxymethyl and isopropyloxycarbonyl phosphoester prodrugs of CDNs was described, and masking the negative charges of CDNs resulted in significantly improved activity of the prodrugs. Rapid uptake and intracellular cleavage of pivaloyloxymethyl prodrugs to the parent CDN were also observed, with peak intracellular concentration reached within 2 hours.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Chemistry, Physical
Yue Liu, Congcong Ma, Gabriela Novakova, Ales Marek, Frantisek Turecek
Summary: Cytidine ribonucleosides were studied in the gas phase with fixed-charge tags to probe the behavior of isolated nucleobase chromophores in different forms. The formation of protonated tautomers and hydrogen-adduct radical forms were observed, with the protonated forms favored under different conditions. Collision-induced dissociations of cytidine radicals were characterized by calculations of transition state structures and energies, revealing them to be more stable than previously reported adenosine and guanosine radicals.
JOURNAL OF PHYSICAL CHEMISTRY A
(2021)
Article
Biochemistry & Molecular Biology
Matej Milacek, Lenka Bittova, Sarka Tumova, Ondrej Luksan, Robert Hanus, Pavlina Kyjakova, Ales Machara, Ales Marek, Marek Jindra
Summary: Juvenile hormone controls insect reproduction and development through a receptor complex consisting of Met and Tai proteins, affecting vitellogenesis in cockroaches and caste polyphenism in termites. Studies show high-affinity binding of JH III by Met proteins in cockroaches and termites, with evidence of JH receptor agonist binding in hemimetabolous species.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Biochemical Research Methods
Tian Yongsong, Bretislav Broz, Frantisek Turecek, Ales Marek
Summary: This study presents a time- and cost-efficient method for tritium late-stage labeling of compounds with a tertiary trifluoroethylsulfonamide moiety. By utilizing hydrogen-isotope exchange reactions in tertiary benzenesulfonamides and iridium catalysts, a high specific activity of the radioligand was achieved. Alternative heterogeneous catalyst conditions provided significant enrichment, but failed when repeating with tritium gas.
JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS
(2022)
Article
Biochemistry & Molecular Biology
Zuzana Honetschlagerova, Lucie Hejnova, Jiri Novotny, Ales Marek, Ludek Cervenka
Summary: The study aimed to evaluate the effects of renal denervation on heart failure, specifically looking at renal vascular responsiveness to angiotensin II and the characterization of angiotensin II receptors in the kidney. The findings suggest that maintained renal AT(1) receptor binding combined with elevated ANG II levels influence renal hemodynamics and lead to renal dysfunction. Renal denervation did not attenuate the decrease in renal blood flow or enhance renal vascular responsiveness to angiotensin II, indicating that the beneficial effects of renal denervation on heart failure mortality may not be primarily mediated by renal mechanisms.
Article
Biochemical Research Methods
Michal Kriegelstein, Ales Marek
Summary: [H-3]NAAG, a widely used substrate in research, has been successfully synthesized with high purity and activity. Due to its unavailability in the market, a reliable synthetic procedure has been developed.
JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS
(2022)
Article
Biochemical Research Methods
Bretislav Broz, Frantisek Turecek, Ales Marek
Summary: Fast and reasonable low-scale (200 nmol) syringe-made synthesis of N-15-labeled oligonucleotides representing DNA trinucleotide codons was achieved using solid-phase controlled pore glass synthesis column technique via the phosphoramidite method. The labeled oligonucleotides have acceptable yields and purity for direct applications in mass spectrometry.
JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS
(2022)
Article
Biochemical Research Methods
Jiahao Wan, Bretislav Broz, Yue Liu, Ales Marek, Shu R. Huang, Frantisek Turecek
Summary: The dissociation of DNA trinucleotide codons in gas-phase ions was investigated by mass spectrometry. The study reveals different patterns of nucleobase loss depending on the nucleobase, as well as the involvement of proton migration and neighboring group participation.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Article
Biochemical Research Methods
Jiahao Wan, Bretislav Broz, Yue Liu, Shu R. Huang, Ales Marek, Frantisek Turecek
Summary: Sixty DNA trinucleotide cation radicals were generated by electron transfer and studied using mass spectrometry and theoretical calculations. The dissociations involved loss of nucleobase molecules, backbone cleavage, and cross-ring fragmentations. Specific features included the formation of d2+ ions, hydrogen atom migrations, and cross-ring cleavages, which depended on the nucleobase type and position.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Biochemistry & Molecular Biology
Anna Kertisova, Lenka Zakova, Katerina Machackova, Ales Marek, Pavel Sacha, Petr Pompach, Jiri Jiracek, Irena Selicharova
Summary: This study investigates the transition between inactive and active states of insulin receptor (IR) and insulin-like growth factor 1 receptor (IGF-1R) through mutation of key residues. The results show that these mutations significantly affect the binding and activation abilities of the receptors, providing further insights into the relationship between the structure and function of these receptors.
Article
Chemistry, Organic
Geeta Devi Yadav, Pooja Chaudhary, Balaram Pani, Surendra Singh
Summary: Chiral transition metal complexes with privileged ligands are efficient catalysts for various asymmetric organic transformations. Transition metal complexes of C1-symmetric pyrrolidine-based ligands have been widely used in asymmetric organic reactions. However, a comprehensive review article on the transition metal complexes of chiral C1-symmetric pyrrolidine-based ligands derived from (L)-proline has not been published.
TETRAHEDRON LETTERS
(2024)
