Article
Chemistry, Multidisciplinary
Yong Woong Jun, Eddy Albarran, David L. Wilson, Jun Ding, Eric T. Kool
Summary: Mitochondrial function declines with aging and neurodegeneration, largely due to accumulated mutations in mtDNA from deficient DNA repair. A fluorescent probe, UBER, was used to visualize mitochondrial BER activity, showing increased repair under oxidative stress and time delay between stress onset and BER initiation. Cells lacking MTH1 exhibited elevated demand for BER activity during extended stress, while absence of OGG1 limited glycosylation capacity.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biochemical Research Methods
Miroslav Kuba, Radek Pohl, Tomas Kraus, Michal Hocek
Summary: Nucleosides and 2'-deoxyribonucleoside triphosphates (dNTPs) containing a bodipy fluorophore attached to position S of 2'-deoxycytidine were synthesized. They exhibited strong fluorescence and sensitivity to viscosity. The modified dNTPs served as substrates for DNA polymerases and were used for enzymatic synthesis of labeled DNA. The modified DNA probes acted as viscosity sensors, responding to protein binding. The dNTP with a longer linker was successfully transported into live cells and incorporated into genomic DNA, enabling DNA staining and imaging of DNA synthesis.
BIOCONJUGATE CHEMISTRY
(2023)
Article
Chemistry, Analytical
Anna Maria Ranieri, Matteo Vezzelli, Kathryn G. Leslie, Song Huang, Stefano Stagni, Denis Jacquemin, Haibo Jiang, Alysia Hubbard, Luca Rigamonti, Elizabeth L. J. Watkin, Mark I. Ogden, Elizabeth J. New, Massimiliano Massi
Summary: New organometallic molecular probes have been developed for bacterial imaging, with the potential to support multiple imaging modalities.
Article
Chemistry, Multidisciplinary
Aaron T. Blanchard, Zi Li, Elizabeth C. Duran, Catherine E. Scull, J. Damon Hoff, Keenan R. Wright, Victor Pan, Nils G. Walter
Summary: DNA-based FluoroCubes were developed as a solution to photobleaching in fluorescence microscopy. These compact FluoroCubes, coupled with up to 6 fluorescent dyes, exhibit enhanced photostability up to 50 times and emit a higher number of photons up to 40 times compared to single dyes. The study addresses the mechanism behind the improved photostability and confirms the compatibility of FluoroCubes with FRET and dark quenching applications.
Review
Chemistry, Multidisciplinary
Fantian Zeng, Sureya Nijiati, Longguang Tang, Jinmin Ye, Zijian Zhou, Xiaoyuan Chen
Summary: Understanding the intricate molecular machinery of ferroptosis and leveraging this knowledge can advance disease prevention, diagnosis, treatment, and prognosis. New approaches for in situ detection of key regulators and events provide a comprehensive view of ferroptosis across different scales. Advanced applications integrating ferroptosis detection with cutting-edge technologies show great potential in ferroptosis research. This review summarizes the mechanisms and regulators of ferroptosis, presents detection approaches and applications, and discusses future prospects and challenges for therapeutic development.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Suji Lee, Min Su Han
Summary: We described an analyte-activatable artificial peroxidase system by caging a dimanganese complex with an analyte-reactive trigger. This system allowed adjustments of the detection target based on the trigger and detection modes, such as fluorescence and colorimetric. It was successfully applied to a versatile enzyme assay for leucine aminopeptidase and gamma-glutamyl transpeptidase based on spectrophotometric change induced from the oxidation of the peroxidase substrate by analyte-triggered peroxidase-like activity.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Siyang Feng, Zhirong Qu, Zhikuan Zhou, Jiaying Chen, Lizhi Gai, Hua Lu
Summary: Two novel Si-bridged meso-annulated BODIPY dyes were prepared through intermolecular C-I silylation and subsequent intramolecular C-H silylation in a one-pot reaction. A marked redshift of the main spectral bands was observed due to efficient sigma*-pi* conjugation, resulting in notable stabilization of the LUMOs. Si-annulation blocks non-radiative decay and contributes to higher fluorescence quantum yields, making this strategy attractive for constructing highly emissive polycyclic aromatic hydrocarbons.
CHEMICAL COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Natalia Grytsyk, Ludovic Richert, Pascal Didier, Dmytro Dziuba, Stefano Ciaco, Viola Mazzoleni, Thiebault Lequeu, Mattia Mori, Yitzhak Tor, Lara Martinez-Fernandez, Roberto Improta, Yves Mely
Summary: Time-resolved fluorescence anisotropy (TRFA) technique provides important information on the dynamics of biomolecules and their interaction with ligands. This study shows that thienoguanosine ((th)G), a fluorescent analogue of guanosine, overcomes the limitations of non-fluorescent natural nucleosides and serves as a unique reporter for investigating the rotational dynamics of dsDNA and protein/dsDNA complexes.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Chemistry, Analytical
Hongwei Yang, Yayun Wu, Hefei Ruan, Feng Guo, Yuxin Liang, Gege Qin, Xiaolong Liu, Zhen Zhang, Jinghe Yuan, Xiaohong Fang
Summary: This study introduces a new type of surface-engineered gold nanoclusters (Au NCs) for super-resolution imaging, which are ultrasmall and ultrabright. With modification on the surface, the fluorescence intensity of Au NCs is enhanced and specific biomolecules can be labeled. These Au NCs show excellent performance and have great potential for applications in STED and DLEM imaging.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Ahsan Ausaf Ali, Yousef Bagheri, Qian Tian, Mingxu You
Summary: The cell membrane is a complex mixture of lipids, proteins, and other components that can selectively interact with each other to control cell signaling. This study introduces new types of DNA zipper probes, which can be used to measure cell membrane dynamic interactions and the formation of lipid domains.
Article
Chemistry, Multidisciplinary
Sandeep Kumar, Sumit Kumar, Jyotirmoy Maity, Banty Kumar, Shilpika Bali Mehta, Ashok K. Prasad
Summary: The Groebke-Blackburn-Bienayame (GBB) reaction is efficiently used for synthesizing novel fluorescent 5-azaindolizino-2'-deoxyuridines from commercially available thymidine. These synthesized compounds show strong fluorescence properties and have potential applications in studying the local structure and dynamics of nucleic acids.
NEW JOURNAL OF CHEMISTRY
(2021)
Article
Chemistry, Analytical
Li Guan, Yanyan Zhou, Xiao Li, Yongbao Mao, Anyang Li, Yile Fu, Wen Liu, Sheying Dong, Zhen Liang, Yukui Zhang, Qun Zhao, Lihua Zhang
Summary: A universal strategy has been developed to synthesize pentamethine cyanines with different aromatic heterocycle substituents on the meso-polymethine chain, leading to the development of specific fluorescent probes with high sensitivity and robust OFF/ON switching for targeting G4s. These probes exhibit self-quenching in aqueous solution due to H-aggregation, but can be triggered to emit fluorescence upon recognition of G-quadruplexes.
ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Bhavna Gowan Gordhan, Carolina Herrera, Azure-Dee Pillay, Thabiso Seiphetlo, Christopher Shawn Ealand, Edith Machowski, Natasha Singh, Ntombexolo Seatholo, Kennedy Otwombe, Limakatso Lebina, Rebecca Frise, Gabriella Scarlatti, Francesca Chiodi, Neil Martinson, Julie Fox, Bavesh Davandra Kana
Summary: In this study, an ex vivo mucosal tissue explant challenge model for studying SARS-CoV-2 infection and replication was established. However, the model failed to support viral replication, possibly due to neutralizing antibodies generated from vaccination.
Article
Chemistry, Multidisciplinary
Kazuto Takaishi, Sho Murakami, Kazuhiro Iwachido, Tadashi Ema
Summary: A series of axially chiral binaphthyls and quaternaphthyls with alternating aromatic fluorophores were synthesized using a divergent method, leading to the formation of unidirectionally twisted exciplex exhibiting circularly polarized luminescence (CPL). These compounds represent the first examples of monomolecular exciplex CPL dyes and the versatile synthesis method also improved fluorescence intensity. The study confirmed that the excimer chirality rule can be applied to exciplex dyes, with left- and right-handed exciplexes showing (-)- and (+)-CPL, respectively, when the twist angle is less than 90 degrees.
Article
Materials Science, Biomaterials
Yan Zhao, Qingxin Yao, Jiali Chen, Ruijia Zhang, Jialei Song, Yuan Gao
Summary: There is an urgent need for a visual drug delivery system in precision medicine. This study constructs a self-reporting bioorthogonal prodrug activation system that interprets prodrug activation events using fluorescence emission. The system selectively forms in tumor cells and can reflect the release of active drugs through measuring fluorescence brightness.
BIOMATERIALS SCIENCE
(2022)
Article
Engineering, Biomedical
Victor Naumenko, Aleksey Nikitin, Anastasiia Garanina, Pavel Melnikov, Stepan Vodopyanov, Ksenia Kapitanova, Daria Potashnikova, Daniil Vishnevskiy, Irina Alieva, Artem Ilyasov, Barbara Z. Eletskaya, Maxim Abakumov, Vladimir Chekhonin, Alexander Majouga
ACTA BIOMATERIALIA
(2020)
Article
Chemistry, Organic
Oleksandr Vyhivskyi, Dimitri N. Laikov, Alexander V. Finko, Dmitry A. Skvortsov, Irina V. Zhirkina, Victor A. Tafeenko, Nikolay V. Zyk, Alexander G. Majouga, Elena K. Beloglazkina
JOURNAL OF ORGANIC CHEMISTRY
(2020)
Article
Chemistry, Medicinal
Dmitry A. Skvortsov, Sophia K. Emashova, Marina A. Kalinina, Olga A. Dontsova
Summary: Delocalized lipophilic cations (DLCs) are molecules known to target mitochondria and may have antioxidant or anticancer properties. The novel cytotoxic fluorescent compound 62E2 exhibits cytotoxic and mitotoxic effects at low concentrations, with some selectivity against A549 cancer cells. Its closest analog, F16, has also been described as a potential anticancer agent.
ARCHIV DER PHARMAZIE
(2021)
Review
Biochemistry & Molecular Biology
Anzhela Pavlova, Elena A. Kubareva, Mayya Monakhova, Maria Zvereva, Nina G. Dolinnaya
Summary: DNA G-quadruplexes are crucial components of regulatory systems in cells and impact genome integrity by impeding DNA replication. Recent studies have focused on the role of G4s in genomic instability and DNA repair processes, highlighting the intricate balance between stability and instability in the genome.
Article
Biochemistry & Molecular Biology
Fedor Grabovenko, Liudmila Nikiforova, Bogdan Yanenko, Andrey Ulitin, Eugene Loktyushov, Timofei Zatsepin, Elena Zavyalova, Maria Zvereva
Summary: Study on nucleic acid aptamers specific to S-protein and its receptor binding domain reveals their sensitivity to glycosylation patterns and biotinylation sites, highlighting the importance of careful testing for new viral variants in the development of specific therapy and biosensors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Alexander N. Malyavko, Olga A. Petrova, Maria Zvereva, Vladimir Polshakov, Olga A. Dontsova
Summary: This study reports the functional characterization of the Rif1 homologue from the methylotrophic thermotolerant budding yeast Hansenula polymorpha DL-1. The study reveals that Rif1 in H. polymorpha suppresses telomerase-dependent telomere elongation through direct DNA binding and association with the Ku heterodimer. It is also found that Rif1 binds Stn1 and promotes its accumulation at telomeres in H. polymorpha.
Article
Chemistry, Multidisciplinary
E. K. Pisarev, O. O. Kapitanova, I. A. Vesolova, M. Zvereva
Summary: This review discusses contemporary approaches to designing sensory systems for the identification and determination of nucleic acids without amplifying target molecules, focusing on methods based on surface plasmon resonance and surface-enhanced Raman spectroscopy, and their possibilities, limitations, and prospects for further development.
MOSCOW UNIVERSITY CHEMISTRY BULLETIN
(2021)
Article
Biochemistry & Molecular Biology
Mayya Monakhova, Elena A. Kubareva, Kirill K. Kolesnikov, Viktor A. Anashkin, Egor M. Kosaretskiy, Maria Zvereva, Elena A. Romanova, Peter Friedhoff, Tatiana S. Oretskaya, Timofei S. Zatsepin
Summary: Covalent protein capture by reactive DNA derivatives allows for investigation of structural features in DNA-protein recognition. The use of acrylamide-modified DNA duplexes provides excellent selectivity for cysteine trapping, making them valuable tools for studying DNA-protein interactions.
Article
Biochemistry & Molecular Biology
Tsimur Hasanau, Eduard Pisarev, Olga Kisil, Naosuke Nonoguchi, Florence Le Calvez-Kelm, Maria Zvereva
Summary: This article reviews the methods used to determine the TERT promoter mutational status in tumoral diseases of the central nervous system. It compares the operational characteristics of the most common methods and their applicability in clinical practice for personalized treatments. The article also discusses the importance of TERT mutational status and other molecular biomarkers in the management of central nervous system tumors.
Article
Chemistry, Multidisciplinary
V. D. Makarova, E. K. Pisarev, S. A. Pavlova, G. Pavlova, M. Zvereva
Summary: This study compared the methods of real-time PCR and digital droplet PCR for detecting mutations in the promoter region of the TERT gene, showing the advantage of digital droplet PCR in analyzing central nervous system tumor lesions. By analyzing mutations in the TERT gene of human brain tumor cell cultures, the mutant allelic fraction for Grade IV glioblastoma IDH1 was determined for the first time.
MOSCOW UNIVERSITY CHEMISTRY BULLETIN
(2022)
Article
Chemistry, Inorganic & Nuclear
Daniil Spector, Alexander Erofeev, Peter Gorelkin, Dmitry Skvortsov, Alexander Trigub, Alina Markova, Vita Nikitina, Nikolay Ul'yanovskiy, Alexander Shtil, Alevtina Semkina, Ksenia Vlasova, Nikolay Zyk, Alexander Majouga, Elena Beloglazkina, Olga Krasnovskaya
Summary: A design of Pt(iv) prodrugs with tumor cell targeting moieties leading to increased selectivity is of interest. Herein, we designed a novel Pt(iv) prodrugs with COX-inhibitor naproxen, long-chain hydrophobic stearic acid moiety and biotin as axial ligands. We have established that for Pt(iv) prodrugs with biotin and naproxen or stearate in axial position, the lipophilicity rather than biotin receptors expression is the main factor of cytotoxicity. We also monitored the reduction speed of Pt(iv) prodrug 3 with naproxen and biotin in axial positions in A549 cells using XANES and demonstrated that the prodrug gradually releases cisplatin within 20 hours of incubation.
DALTON TRANSACTIONS
(2023)
Article
Chemistry, Organic
Georgy L. Karetnikov, Dmitry A. Skvortsov, Anastasiia A. Moseicheva, Nikolay V. Zyk, Oksana B. Bondarenko
Summary: An efficient synthesis route for new hybrid isoxazole-triazole compounds was developed starting from 2-aryl-1,1-dichlorocyclopropanes or N-hydroxybenzimidoyl chlorides, achieving high yields and excellent regioselectivity. The synthesized compounds were evaluated for their biological activity.
ASIAN JOURNAL OF ORGANIC CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Alexander Finko, Anatolii Sokolov, Dmitry A. Guk, Victor A. Tafeenko, Anna A. Moiseeva, Dmitry A. Skvortsov, Andrei A. Stomakhin, Andrei A. Beloglazkin, Roman S. Borisov, Vladimir Pergushov, Mikhail Ya Melnikov, Nikolay Zyk, Alexander G. Majouga, Elena K. Beloglazkina
Summary: A series of new organic ligands consisting of two 5-(2-pyridylmethylene)-3,5-dihydro-4H-imidazol-4-one units linked with polymethylene chains of various lengths have been synthesized. These ligands react with copper salts to form mono- and binuclear complexes containing Cu2+ or Cu1+, showing higher cytotoxicity and reasonable selectivity to cancer cell lines.
Article
Chemistry, Inorganic & Nuclear
Olga Krasnovskaya, Daniil Spector, Alexander Erofeev, Peter Gorelkin, Roman Akasov, Dmitry Skvortsov, Alexander Trigub, Ksenia Vlasova, Alevtina Semkina, Nikolay Zyk, Elena Beloglazkina, Alexander Majouga
Summary: Researchers reported two multispecific Pt-IV complexes, DNP and NP, with the non-steroidal anti-inflammatory drug naproxen (NPX) as the axial ligand(s). The study clarified the mechanism of action of DNP, its therapeutic target, and intracellular redox-status.
DALTON TRANSACTIONS
(2021)
Article
Chemistry, Multidisciplinary
Vadim Filatov, Maksim Kukushkin, Juliana Kuznetsova, Dmitry Skvortsov, Viktor Tafeenko, Nikolay Zyk, Alexander Majouga, Elena Beloglazkina
Article
Chemistry, Organic
Geeta Devi Yadav, Pooja Chaudhary, Balaram Pani, Surendra Singh
Summary: Chiral transition metal complexes with privileged ligands are efficient catalysts for various asymmetric organic transformations. Transition metal complexes of C1-symmetric pyrrolidine-based ligands have been widely used in asymmetric organic reactions. However, a comprehensive review article on the transition metal complexes of chiral C1-symmetric pyrrolidine-based ligands derived from (L)-proline has not been published.
TETRAHEDRON LETTERS
(2024)
