期刊
TETRAHEDRON
卷 68, 期 3, 页码 857-864出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tet.2011.11.041
关键词
CuAAC click chemistry; Reverse-click oligonucleotide labeling; Cyanine dyes; Fluorescent oligonucleotide probes; HyBeacons in genetic analysis
资金
- ATDBio Ltd.
- BBSRC [BB/J001694/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/J001694/1] Funding Source: researchfish
A convenient method of oligonucleotide labeling using click chemistry has been developed. A 2'-mesyloxyethyl ribothymidine phosphoramidite monomer was incorporated into DNA at several loci during solid phase oligonucleotide synthesis and converted to 2'-azidoethyl ribothymidine in high yield on the synthesis resin. The resultant azide oligonucleotides were doubly and triply labeled with alkynemodified cyanine dyes and their biophysical properties were studied. The influence of the dye structures and method of labeling on the fluorescence properties of the DNA probes is discussed and compared with a standard labeling method using active esters of Cy-Dyes. (C) 2011 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据