期刊
TALANTA
卷 129, 期 -, 页码 411-416出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2014.06.017
关键词
Sandwich-type electrochemical immunosensor; Alpha fetoprotein; MCM-41; Toluidine blue; Gold nanoparticles
资金
- National Natural Science Foundation of China [21175057, 21375047, 21377046]
- Science and Technology Plan Project of Jinan [201307010]
A novel and ultrasensitive sandwich-type electrochemical immunosensor was designed for the quantitative detection of alpha fetoprotein (AFP) using multifunctional mesoporous silica (MCM-41) as platform and label for signal amplification. MCM-41 has high specific surface area, high pore volume, large density of surface silanol groups (Si-OH) and good biocompatibility. MCM-41 functionalized with 3-aminopropyltriethoxysilane (APTES), gold nanoparticles (Au NPs) and toluidine blue (TB) could enhance electrochemical signals. Moreover, primary antibodies (Ab(1)) and secondary antibodies (Ab(2)) could be effectively immobilized onto the multifunctional MCM-41 by the interaction between Au NPs and amino groups (-NH2) on antibodies. Using multifunctional MCM-41 as a platform and label could greatly simplify the fabrication process and result in a high sensitivity of the designed immunosensor. Under optimal conditions, the designed immunosensor exhibited a wide liner range from 10(-4) ng/mL to 10(3) ng/mL with a low detection limit of 0.05 pg/mL for AFP. The designed immunosensor showed acceptable selectivity, reproducibility and stability, which could provide potential applications in clinical monitoring of AFP. (C) 2014 Elsevier B.V. All rights reserved.
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