期刊
TALANTA
卷 118, 期 -, 页码 14-20出版社
ELSEVIER
DOI: 10.1016/j.talanta.2013.09.036
关键词
MagCNTs@TiO2 composites; Selective enrichment; Phosphopeptides; Mass spectrometry analysis
资金
- National Basic Research Priorities Program [2012CB910601, 2013CB911201]
- National Natural Science Foundation of China [21075022, 21275033, 21105016]
- Fudan University
- Research Fund for the Doctoral Program of Higher Education of China [20110071110007, 201000711 20053]
- Shanghai Municipal Natural Science Foundation [11ZR1 403200]
- Shanghai Leading Academic Discipline Project [B109]
Selective enrichment of phosphoproteins or phosphopeptides from complex mixtures is essential for mass spectrometry (MS)-based phosphoproteomics. In this work, for the first time, titania nanoparticles coated magnetic carbon nanotubes (denoted as MagCNTs@TiO2 composites) were synthesized through a facile but effective solvothermal reaction for selective enrichment of phosphopeptides. The MagCNTs@TiO2 material demonstrated low limit of detection (20 fmol), along with an exceptional great specificity to capture phosphopeptides from a tryptic digest of the mixture of a nonphosphorylated protein BSA and a phosphorylated protein beta-casein with molar ratios of BSA/beta-casein up to 200:1. In addition, the high magnetic susceptibility allowed convenient separation of the target peptides by magnetic separation. Experimental results demonstrated that the MagCNTs@TiO2 composites showed excellent potential for the selective enrichment of phosphopeptides for MS analysis. (C) 2013 Elsevier B.V. All rights reserved.
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