期刊
TALANTA
卷 105, 期 -, 页码 93-100出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2012.11.058
关键词
Enzyme biosensors; Sucrose; Continuous flow system; Multiplexed sugar detection
资金
- Spanish Ministerio de Ciencia e Innovacion Research Project [CTQ2009-09351BQU]
- AVAN-SENS Program from the Comunidad de Madrid [S2009PPQ-1642]
An integrated amperometric sucrose biosensor involving a 3-mercaptopropionic acid (MPA) self-assembled monolayer (SAM)-modified gold disk electrode (AuE) and coimmobilization of the enzymes invertase (INV) and fructose dehydrogenase (FDH) as well as the redox mediator tetrathiafulvalene (TTF) by means of a dialysis membrane is reported. Amperometry in stirred solutions at a detection potential of +0.10 V provided a linear calibration plot for sucrose over the 1.2 x 10(-6)-3.0 x 10(-3) mol L-1 concentration range, with a limit of detection of 3.6 x 10(-7) mol L-1. The practical usefulness of the biosensor was demonstrated by determining sucrose in condensed milk and in an infant food reference material with good results. Additionally, the biosensor was implemented together with commercial fructose and glucose amperometric biosensors in a continuous flow system to perform the multiplexed quantification of sucrose, fructose and glucose in a single experiment. The operational characteristics of the biosensors in this novel flow system were evaluated and their applicability was demonstrated through the simultaneous determination of the three sugars in the above-mentioned reference material. (C) 2012 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据