期刊
TALANTA
卷 115, 期 -, 页码 642-651出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2013.05.052
关键词
Carbohydrates; Derivatization; GC/MS; Ethoximation; Retention index; Trimethylsilylation
资金
- Christian Doppler Research Society (CD lab Advanced Cellulose Chemistry and Analytics)
- Lenzing AG, Austria
The qualitative and quantitative analysis of complex carbohydrate mixtures is a challenging problem. When tackled by GC/MS, close retention times and largely similar mass spectra with no specific features complicate unambiguous identification, especially of monosaccharides. An optimized pre-capillary ethoximation-silylation GC/MS method for determination of monosaccharides and disaccharides was applied to a wide range of analytes (46 compounds). The two-step derivatization resulted in a pair of syn and anti peaks with specific retention and intensity ratio. The resulting dataset of mass spectra was subjected to a PCA-based pattern recognition. An oxime peak identifier (OPI) of the carbohydrate analytes, based on the combination of an internal standard and the corresponding syn/anti peak ratios, increased the reliability of the identification of reducing carbohydrates. Finally, the introduced EtOx-TMS derivatization method was applied to four different carbohydrate matrices (agave sirup, maple sirup, palm sugar, and honey). (C) 2013 Elsevier B.V. All rights reserved.
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