Review
Chemistry, Multidisciplinary
Kate Moghadam, Diane Beauchemin
Summary: To analyze trace evidence at crime scenes, non-destructive or low-sample analysis techniques are preferred. One such technique is solid sampling ETV-ICPOES, which requires only a small amount of sample. This article discusses the capabilities of ETV-ICPOES in forensic analysis and presents various methods for analyzing physical evidence. Internal standardization and multivariate analysis are used to compensate for sample loading effects and enable quantification and discrimination of evidence. The article also explores future perspectives for utilizing ETV-ICPOES in forensic settings.
CHEMICAL COMMUNICATIONS
(2023)
Article
Materials Science, Multidisciplinary
Corliss Kin Sio, Teresa Baumer, James Cahill, Sarah Hansen, Sharee Harris, Josh Wimpenny, Rachel Lindvall, Wyatt Du Frane, Josh Kuntz
Summary: The phase stability of cBN is influenced by the presence of impurities, but quantitative analysis methods are currently lacking. This study presents a flux fusion method for fully digesting cBN samples and analyzing impurities using ICPMS. High levels of Si impurities were found in all cBN powders studied.
DIAMOND AND RELATED MATERIALS
(2022)
Article
Biochemical Research Methods
Yinyin Tang, Lihong Liu, Qiying Nong, Hua Guo, Qinfei Zhou, Dingyi Wang, Yongguang Yin, Jianbo Shi, Bin He, Ligang Hu, Guibin Jiang
Summary: In this study, a sensitive method based on size exclusion chromatography coupled with inductively coupled plasma-mass spectrometry (SEC-ICP-MS) was developed for the separation and determination of metalloproteins in salt-rich matrices. The method combined with the high matrix introduction (HMI) mode showed good precision and repeatability, and successfully separated and detected lead-binding proteins in real biological tissue samples.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemistry & Molecular Biology
Shuang-Qing Zhang, Sui-Han Cheng, Shi Shen, Bi-Ying Luo, Yan Zhang
Summary: A new method was developed for quantifying Cr(III) and Cr(VI) in Chromium-enriched yeast, using ion chromatography-inductively coupled plasma mass spectrometry. Cr(III) and Cr(VI) were extracted using ultrasound-assisted weakly alkaline EDTA solution combined with boiling, and separated on two successive anion-exchange columns. The method was sensitive, accurate, and precise, with no Cr(VI) species found in CrY.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2021)
Article
Multidisciplinary Sciences
Mateusz Maciejewski, Caroline Sands, Nisha Nair, Stephanie Ling, Suzanne Verstappen, Kimme Hyrich, Anne Barton, Daniel Ziemek, Matthew Lewis, Darren Plant
Summary: Methotrexate is a common first-line treatment for rheumatoid arthritis, but it is ineffective for a significant portion of patients, with no reliable way to predict individual responses. Statistical models based on serum lipid levels were explored for predicting methotrexate response in patients, but clinical covariates alone outperformed lipid-based models in predicting response. Serum lipid profiles at pre-treatment or early-treatment time points are unlikely to be predictive of methotrexate response by 6 months in a way that would be useful for clinical management of rheumatoid arthritis.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Analytical
Yanqun Yang, Yipeng Huang, Zhuqiang Wu, Rui Shi, Zhengyi Chen, Guihua Ruan
Summary: In this work, a porous capillary monolithic column was prepared by thiol-alkyne click polymerization in a fused-silica capillary. The column exhibits excellent permeability and high porosity, allowing for efficient adsorption of trace estrogens via pi-pi interaction and hydrophobic interaction. This method, combined with ultrahigh performance liquid chromatography-tandem mass spectrometry, provides a simple and reliable approach for the determination of eight estrogens in complex biological and environmental samples.
ANALYTICA CHIMICA ACTA
(2022)
Article
Spectroscopy
Peng Li, Yu-Ying Huang, Jun Zeng, Zheng-Zhong Lin, Zhi-Yong Huang
Summary: The study successfully investigated cadmium species in marine shellfish using SEC-ICP-MS and SEC-ESI-Q-TOF-MS methods, revealing multiple cadmium binding species in clams, including high molecular weight Cd-binding molecules and MTs-like Cd-binding proteins.
SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY
(2021)
Article
Chemistry, Analytical
Yu-ki Tanaka, Shunsuke Shimazaki, Yasunori Fukumoto, Yasumitsu Ogra
Summary: A rapid and precise quantification method for a histidine-tagged recombinant protein produced in Escherichia coli (E. coli) has been developed using single-cell inductively coupled plasma-mass spectrometry (SC-ICP-MS). This method allows for the determination of protein quantity based on the signal of nickel or cobalt ions bound to the His-tag, without the need for extraction and purification processes.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Omar Martinez-Mora, Kristof Tirez, Filip Beutels, Wilfried Brusten, Luis F. Leon-Fernandez, Jan Fransaer, Xochitl Dominguez-Benetton, Milica Velimirovic
Summary: In this study, the authors proposed a comprehensive approach that combines conventional sizing techniques and innovative single-particle mass spectrometry to investigate Pt-Pd alloy nanoparticle clusters. This approach provides rapid and accurate characterization of the particle size and distribution of Pt-Pd alloy NCs, enhancing our understanding of bimetallic nanoparticle synthesis and facilitating tailored applications for these materials.
Article
Biochemical Research Methods
Maria S. Jimenez, Mariam Bakir, Daniel Isabal, Maria T. Gomez, Josefina Perez-Arantegui, Juan R. Castillo, Francisco Laborda
Summary: In this study, hydrodynamic chromatography coupled to inductively coupled plasma mass spectrometry was used to simultaneously determine dissolved and nanoparticulate species of gold and silver, with optimized mobile phase for resolution and recovery. The developed methods allowed for quick and accurate detection of nanoparticulate and dissolved species of gold and silver in dietary supplements, showing good agreement with other analysis methods. Due to achievable resolution, the quality of quantitative results is influenced by the relative abundance and size of nanoparticles present.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Jose Grau, Maria Moreno-Guzman, Luis Arruza, Miguel angel Lopez, Alberto Escarpa, Alberto Chisvert
Summary: Miniaturized magnetic-based pipette tip microextraction is a sample preparation approach for microsamples. It involves dispersing a small amount of magnetic sorbent material in a low-volume sample to trap the target analytes, followed by using a pipette tip with a neodymium magnet inside to retrieve the sorbent. The method was successfully applied to determine free cortisol in serum and urine samples from low birth weight preterm newborns.
Article
Chemistry, Multidisciplinary
Jinshun Cang, Weixi Gu, Yuheng Zhang, Xiashi Zhu
Summary: This study developed a magnetic solid extractant coated with amino acid ionic liquid and graphene oxide for the speciation analysis of Cr(iii) and Cr(vi) in water samples using ICP-OES. The method showed good linear ranges and detection limits under optimal conditions, and its effectiveness was validated through quantitative analysis of environmental water samples. This method reveals promising potential in the field of environmental remediation.
NEW JOURNAL OF CHEMISTRY
(2022)
Review
Chemistry, Analytical
A. Fernez Asensio, E. Alvarez-Gonzalez, A. Rodriguez, L. M. Sierra, E. Blanco-Gonzalez
Summary: Dysregulation of TCA cycle enzymes leads to abnormal accumulation of oncometabolites, which contribute to cancer formation and progression. GC-MS and LC-MS are powerful analytical tools for accurate identification and determination of these metabolites.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Luyao Liu, Bin Zhu, Zheng Fang, Na Zhang, Hongqiang Qin, Zhimou Guo, Xinmiao Liang, Zhenzhen Yao, Mingliang Ye
Summary: Bottom-up proteomics is increasingly used in clinical research, but glycoproteomic analysis has been a bottleneck. An automated glycopeptide enrichment method was developed for site-specific N-glycoproteome analysis, showing higher efficiency and reliability.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Liang Fu, Hualin Xie, Jianhua Huang, Xianhua Chen, Lin Chen
Summary: This study introduces a novel strategy for determining ultra-trace Ti in human serum using ICP-MS/MS, successfully eliminating interference and achieving high sensitivity. The proposed method was evaluated for accuracy and reliability through spike recovery experiments and comparative analysis, confirming its effectiveness for accurate determination of ultra-trace Ti in serum samples.
ANALYTICA CHIMICA ACTA
(2021)
Article
Materials Science, Multidisciplinary
Vito Kovac, Borut Poljsak, Matic Bergant, Janez Scancar, Uros Mezeg, Jasmina Primozic
Summary: This study assessed the composition of released metal ions from fixed orthodontic appliances in both in vitro and in vivo settings, and found differences in the metal ion content between these two environments. The concentration of released metal ions from all tested alloys was below the upper recommended limit of daily intake, but possible local effects in terms of hypersensitivity cannot be excluded even with such concentrations.
Article
Biochemistry & Molecular Biology
Vito Kovac, Matic Bergant, Janez Scancar, Jasmina Primozic, Polona Jamnik, Borut Poljsak
Summary: This study investigated the metal composition and release of metal ions from orthodontic alloys, as well as the oxidative consequences that the metal ions may cause in the cell. The results showed that beta titanium alloy released the fewest metal ions and did not cause oxidative stress or protein damage. Stainless steel and cobalt-chromium alloy, on the other hand, can cause oxidative stress and protein damage at high concentrations. All metal ions from orthodontic alloys alter the activity of antioxidant enzymes in some way.
Article
Multidisciplinary Sciences
Helena Cindric, Gorana Gasljevic, Ibrahim Edhemovic, Erik Brecelj, Jan Zmuc, Maja Cemazar, Alenka Seliskar, Damijan Miklavcic, Bor Kos
Summary: This study investigated electroporation in the liver using a numerical mesoscale tissue model. The results showed that liver microstructure does not significantly affect the electric field distribution, but major hepatic vessels and portal spaces do have a significant impact, which should be considered in treatment planning.
SCIENTIFIC REPORTS
(2022)
Article
Oncology
Federica Tonon, Maja Cemazar, Urska Kamensek, Cristina Zennaro, Gabriele Pozzato, Sergio Caserta, Flora Ascione, Mario Grassi, Stefano Guido, Cinzia Ferrari, Laura Cansolino, Francesco Trotta, Biljana Grcar Kuzmanov, Giancarlo Forte, Fabiana Martino, Francesca Perrone, Riccardo Bomben, Valter Gattei, Nicola Elvassore, Erminio Murano, Nhung Hai Truong, Michael Olson, Rossella Farra, Gabriele Grassi, Barbara Dapas
Summary: The study demonstrates that 5-Aza can impair the development of HCC by upregulating miR-139-5p, which then affects the proliferative and migratory pathways of HCC.
Article
Biochemistry & Molecular Biology
Monika Savarin, Katarina Znidar, Gregor Sersa, Tilen Komel, Maja Cemazar, Urska Kamensek
Summary: Targeting specific endothelial cell markers involved in different signaling pathways for tumor radiosensitization shows promising potential. We evaluated a plasmid for silencing two prominent targets and explored the therapeutic potential of gene electrotransfer-mediated delivery of this plasmid in an in vivo study. The results demonstrate significant radiosensitization and a good therapeutic potential, making it a promising novel treatment modality for a wide range of tumors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Janez Benedik, Barbara Ogorevc, Simona Kranjc Brezar, Maja Cemazar, Gregor Sersa, Ales Groselj
Summary: This study compared continuous intravenous sedation with general anesthesia in patients undergoing electrochemotherapy, finding that continuous intravenous sedation required lower propofol dosage, shorter anesthesia duration, and higher patient satisfaction.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemical Research Methods
Helena Plesnik, Masa Bosnjak, Maja Cemazar, Gregor Sersa, Tina Kosjek
Summary: The analysis of highly polar agents in cancer treatment is challenging due to their physicochemical properties. In this study, we focused on the analysis of polar cytotoxic bleomycin, which is a mixture of complexing congeners with high molecular mass. We optimized and validated an analytical method for its determination in pharmaceutical and biological specimens.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Sara Milicevic, Maja Cemazar, Andreja Klevisar Ivancic, Gorana Gasljevic, Masa Bosnjak, Gregor Sersa, Barbara Peric
Summary: Cutaneous melanoma, a highly aggressive form of skin cancer, has seen improved treatment with the development of immune checkpoint inhibitors (ICIs). The tumor microenvironment (TME) plays a crucial role in the success of ICIs, and understanding its interactions is important for optimizing their efficacy. Electrochemotherapy (ECT) has been shown to enhance ICIs by inducing tumor cell death, releasing tumor antigens, and modulating the TME. This review summarizes the knowledge of TME changes after ECT in melanoma treatment and highlights differences in tumor-infiltrating immune cells between immunocompetent and immunosuppressed organisms, as well as the potential benefits of ECT for organ transplant recipients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Ziva Modic, Maja Cemazar, Bostjan Markelc, Andrej Cor, Gregor Sersa, Simona Kranjc Brezar, Tanja Jesenko
Summary: This study successfully developed a transplantable immunocompetent mouse model of HPV-positive OSCC and characterized it in vitro and in vivo. The results showed that this model exhibited increased radiosensitivity compared to traditional OSCC mouse models and can be used to explore immune-based treatment approaches.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Immunology
Barbara Lisec, Bostjan Markelc, Katja Ursic Valentinuzzi, Gregor Sersa, Maja Cemazar
Summary: This study compared the effectiveness of CaEP and ECT, and investigated the impact of the combination of CaEP and IL-12 GET on tumors. The results showed that CaEP was more effective than ECT in vitro and in the 4T1 tumor model, while the efficacy of CaEP and ECT was similar in the B16-F10 tumor model. Furthermore, the combination of CaEP and IL-12 GET could modify tumor immune cells and vasculature, further enhancing the antitumor effect.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Oncology
Gregor Vivod, Masa Bosnjak, Nina Kovacevic, Gregor Sersa, Sebastjan Merlo, Maja Cemazar
Summary: This prospective institutional study aimed to determine the safety and feasibility of electrochemotherapy in the treatment of vulvar cancer recurrence. The results showed that electrochemotherapy is safe and feasible for the treatment of local vulvar cancer recurrence for nonpalliative purposes.
Review
Pharmacology & Pharmacy
Tanja Jesenko, Simona Kranjc Brezar, Maja Cemazar, Alice Biasin, Domenico Tierno, Bruna Scaggiante, Mario Grassi, Chiara Grassi, Barbara Dapas, Nhung Hai Truong, Michela Abrami, Fabrizio Zanconati, Deborah Bonazza, Flavio Rizzolio, Salvatore Parisi, Giorgia Pastorin, Gabriele Grassi
Summary: Hepatocellular carcinoma (HCC) is a major global health challenge, ranking third in cancer deaths worldwide. Despite recent therapeutic advances, prognosis remains poor, necessitating the development of novel strategies. This study focuses on targeting molecules with aberrant expression confined to tumor cells, particularly non-coding RNAs (ncRNAs) such as miRNAs, lncRNAs, and circRNAs. These ncRNAs play a significant role in regulating various aspects of HCC, including proliferation, apoptosis, invasion, and metastasis. The review provides the latest state-of-the-art approaches, highlighting key trends and opportunities for advanced and efficacious HCC treatments.
Article
Immunology
Ursa Kesar, Bostjan Markelc, Tanja Jesenko, Katja Ursic Valentinuzzi, Maja Cemazar, Primoz Strojan, Gregor Sersa
Summary: Electrochemotherapy (ECT) is a clinically acknowledged method that combines the use of anticancer drugs and electrical pulses. It has been found that ECT can induce immunogenic cell death (ICD) in certain situations. However, the universality of this effect across different cancer types and other clinically relevant chemotherapeutics used with ECT remains unknown.
Article
Immunology
Ursa Lampreht Tratar, Nina Milevoj, Maja Cemazar, Katarina Znidar, Katja Ursic Valentinuzzi, Andreja Brozic, Katerina Tomsic, Gregor Sersa, Natasa Tozon
Summary: This study compared the intratumoral (i.t.) and peritumoral (peri.t.) routes of interleukin-12 (IL-12) gene electrotransfer (GET) in combination with electrochemotherapy (ECT). The results showed that intratumoral administration had better treatment outcomes, with significantly improved local tumor control, longer disease-free interval, and progression-free survival compared to the other routes.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Oncology
Gregor Vivod, Tanja Jesenko, Gorana Gasljevic, Nina Kovacevic, Masa Bosnjak, Gregor Sersa, Sebastjan Merlo, Maja Cemazar
Summary: Electrochemotherapy is a effective treatment for vulvar cancer, but some tumors do not respond to it. This study suggests that the vascularity of the tumor prior to treatment may predict the response to electrochemotherapy.
RADIOLOGY AND ONCOLOGY
(2023)
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).
