Article
Chemistry, Multidisciplinary
Kaoru Kioka, Nagi Mizutani, Nobuhiko Hosono, Takashi Uemura
Summary: This paper proposes a design approach using mixed metal-organic frameworks (MOFs) as tunable stationary phases in liquid chromatography (LC). By systematically designing ligands, the pore sizes in the MOFs can be tuned. Columns packed with the MOFs and their mixed-particle/solid-solution stationary phases are prepared and tested for the retention capability of polyethylene glycol (PEG) in LC. The results demonstrate that mixed-linker solid-solution MOFs have higher retention capability compared to pure MOF stationary phases, due to their specific nanostructures.
Article
Chemistry, Analytical
Xiao-Dong Cheng, Zheng Zhang, Yun-Ping Li
Summary: Green chromatography techniques using low-toxic mobile phase and developing stationary phases with adequate retention and separation under high content water mobile phase have gained increasing attention. In this study, an undecylenic acid-bonded silica stationary phase (UAS) was successfully prepared using thiol-ene click chemistry. The synthesized UAS demonstrated excellent separation ability towards highly polar compounds under the mobile phase of high content water, surpassing commercial C18 and silica stationary phases. Overall, this UAS stationary phase meets the requirements of green chromatography and shows promise for enhanced separation.
ANALYTICA CHIMICA ACTA
(2023)
Review
Chemistry, Analytical
Yan Wu, Ning Zhang, Kaixing Luo, Yanjuan Liu, Zhengwu Bai, Sheng Tang
Summary: This article reviews the recent advances in efficient chromatographic separation materials developed in 2020-2021, focusing on their synthesis strategies, performance evaluation, and applications in hydrophilic interaction chromatography and chiral separation. The review provides readers with a comprehensive overview of the significance of these materials in current and future separation technologies.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Inorganic & Nuclear
Anna A. Kotova, Didier Thiebaut, Jerome Vial, Antoine Tissot, Christian Serre
Summary: Analytical chemistry is crucial for separating complex mixtures, and Metal-organic frameworks (MOFs) are promising porous hybrid solids with unique physico-chemical properties, which can be applied in chromatography and solid-phase extraction to address challenges in these fields.
COORDINATION CHEMISTRY REVIEWS
(2022)
Review
Chemistry, Physical
Mikolaj Dembek, Szymon Bocian
Summary: Industrial research, including pharmaceutical research, is using liquid chromatography techniques more frequently. However, the generation of large quantities of hazardous and toxic organic waste has become a concern. In response, green chemistry proposes solutions such as using pure water as a mobile phase for chromatographic separation, which requires new stationary phases or specific chromatographic conditions.
Review
Chemistry, Analytical
Anna W. Sobanska
Summary: This article describes several newly synthesized or forgotten silica-based stationary phases, as well as biologically-based stationary phases. Additionally, packing materials involving macrocyclic chemistry are discussed.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Meichao Xie, Kaijun Quan, Hui Li, Bei Liu, Jia Chen, Yongliang Yu, Jianhua Wang, Hongdeng Qiu
Summary: A new strategy using non-porous silica (NPS) spheres as support and covalent organic frameworks (COFs) as porous functional shell was developed for liquid chromatography, ensuring the independent effect of COFs on separation. NPS@TPB-DMTP was prepared as proof of concept using 1,3,5-tris(4-aminophenyl)benzene (TPB) and 2,5-dimethoxy-1,4-benzenedicarboxaldehyde (DMTP) as monomers by in situ polymerization on the surface of NPS. This new approach of developing COF-based stationary phases will aid in understanding the impact of COFs on separation.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Medicinal
Takafumi Onishi, Weston J. Umstead
Summary: The use and applicability of Cannabis and Cannabis-derived products have increased significantly in the past 5 years. This study focuses on the separation of a 10-cannabinoid mixture using chiral stationary phases and appropriate single-peak identification.
Review
Chemistry, Analytical
Isabela de Paula Lima, Sandy Polycarpo Valle, Marcone Augusto Leal de Oliveira, Flavia Ferreira de Carvalho Marques, Fernando Antonio Simas Vaz
Summary: Monolithic stationary phases have been widely studied in separation science, particularly in liquid chromatography and capillary electrochromatography, for over three decades. The production of monolithic columns involves various steps, including precursor solution preparation, tubing pretreatment, solution introduction, in situ reaction, and posttreatment. Different types of processes and reactions, such as sol-gel, polycondensation, free radical polymerization, and click reactions, are discussed. This work aims to provide an overview of the production process and the characteristics of monolith compositions, as well as to encourage researchers in finding suitable monoliths for analytical separations.
MICROCHEMICAL JOURNAL
(2023)
Article
Biochemical Research Methods
Yuanyuan Pei, Xinyu Li, Guangjian Zeng, Yuting Gao, Tao Wen
Summary: Two novel chiral stationary phases (CSPs) based on lactide derivatives, PMLA and N-PMLA, showed better chiral recognition ability compared to PLLA in both reversed-phase and normal-phase chromatographic modes in this study.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Multidisciplinary
Yongrui He, Xiaohong Yang, Meiling Qi, Chuanfeng Chen
Summary: The new triptycene-derived oxacalixarene (TDOC) demonstrated excellent separation performance and high thermal stability as a stationary phase for gas chromatography, showing promise for separating various analytes and isomers.
CHINESE CHEMICAL LETTERS
(2021)
Article
Chemistry, Analytical
Ang Li, Song Xue, Siru Ren, Yu Xu, Qi Zhang
Summary: Research on the application of deep eutectic solvents (DESs) in capillary electrophoresis (CE) is still in the early stage, and there is controversy over their true role. This study established a CE system using a hydrophobic DES as a pseudo-stationary phase (PSP). The hydrophobic DES formed stable aggregates and exhibited impressive selectivities through a hydrophobic mechanism.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemical Research Methods
Wei Chen, Ji-Zhou Jiang, Guo-Song Qiu, Sheng Tang, Zheng-Wu Bai
Summary: The present study aimed to investigate the interactions between chitosan-type chiral selectors and chiral analytes in enantioseparation. Through synthesizing and characterizing chitosan derivatives and evaluating their enantioseparation capability, the study highlighted the importance of substituents on the phenyl group in chiral recognition mechanism. The study also shed light on the role of hydrogen bonding formation in enantioseparation and the impact of different mobile phases on the process.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemical Research Methods
Mingliang Zhang, Yujie Zhang, Shouyong Zhou, Hui Zhong, Haifeng Han, Hongdeng Qiu
Summary: Among the three isomers, p-terphenyl is particularly useful for the construction of optical devices due to its unique structure. This study presents two rigid stationary phases bearing p-terphenyl as an external moiety and variable embedded carbamate groups, which exhibit multiple retention mechanisms, high specificity to linear analytes, and distinctive chromatographic properties.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Review
Biochemistry & Molecular Biology
Handaja Rusli, Rindia M. Putri, Anita Alni
Summary: Compound separation is crucial in the production and analysis of chemical compounds. This review discusses the recent advancements in liquid chromatography for separating macromolecule proteins and small organic compounds.
Article
Cell Biology
Urszula Doboszewska, Katarzyna Socala, Mateusz Pierog, Dorota Nieoczym, Jan Sawicki, Adam Sajnog, Bernadeta Szewczyk, Katarzyna Mlyniec, Ireneusz Sowa, Danuta Baralkiewicz, Piotr Wlaz
Summary: The G-protein coupled receptor 39 (GPR39) is a potential target for future drugs, and its pharmacology is not fully understood. Zinc acts as an endogenous agonist or an allosteric modulator for GPR39, while TC-G 1008 is a synthetic agonist. Zinc also enhances the activity of TC-G 1008 at GPR39. Activation of GPR39 by TC-G 1008 promotes epileptogenesis in an epilepsy model and decreases seizure threshold in a seizure test. The amount of zinc in the diet contributes to the effects of TC-G 1008 in vivo.
Article
Biochemistry & Molecular Biology
Magdalena Wojciak, Aleksandra Ziemlewska, Martyna Zagorska-Dziok, Zofia Niziol-Lukaszewska, Dariusz Szczepanek, Tomasz Oniszczuk, Ireneusz Sowa
Summary: This study aimed to evaluate the antioxidant, anti-inflammatory, and protective effects of Sambucus nigra fruit extract and its ferment obtained through fermentation with kombucha tea fungus. The chemical composition of fermented and non-fermented extracts was compared using HPLC/ESI-MS chromatographic method. The antioxidant activity of the samples was assessed using DPPH and ABTS assays. The results showed that the extract and the ferment possess antioxidant properties and promote cell proliferation in both fibroblast and keratinocyte skin cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Jaromir Jarecki, Izabela Polkowska, Waldemar Kazimierczak, Magdalena Wojciak, Ireneusz Sowa, Slawomir Dresler, Tomasz Blicharski
Summary: The aim of this study was to assess the impact of physical activity on the musculoskeletal system in an animal model of early degenerative knee joint lesions. The results showed that compared to inactive rats, active rats had decreased bone density, reduced weight of fat and muscle tissues, indicating the impact of physical activity on bone mineralization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Nutrition & Dietetics
Piotr Bawiec, Jan Sawicki, Paulina Lasinska-Pracuta, Marcin Czop, Ireneusz Sowa, Katarzyna Ilowiecka, Wojciech Koch
Summary: Bioavailability refers to the fraction of a substance that is absorbed from the gastrointestinal tract and enters the bloodstream. This study aimed to assess the bioavailability of selenium (Se) from dietary supplements and explore the influence of diet type on relative bioavailability. The research used a two-stage in vitro digestion model to simulate the digestion of food containing dietary supplements. Selenium bioavailability ranged from 19.31% to 66.10% in the presence of food matrix. Sodium selenate had the highest bioavailability, followed by organic forms and sodium selenite. The basic diet with moderate protein and high carbohydrate and fiber content positively influenced Se bioavailability. The pharmaceutical form of the product also affected bioavailability, with tablets having the highest bioavailability, followed by capsules and coated tablets.
Article
Biochemistry & Molecular Biology
Marcin Feldo, Magdalena Wojciak, Slawomir Dresler, Pawel Sowa, Bartosz J. Plachno, Dariusz Samborski, Ireneusz Sowa
Summary: Chronic venous disease (CVD) affects up to 30% of the global population and is characterized by disturbances in the microcirculation of veins. Diosmin, a phlebotropic drug, has been used in the treatment of CVD, but its molecular mechanism of action is not fully understood. In this study, patients were administered diosmin and biochemical assays were used to evaluate its effects on oxygen management, angiogenesis, and inflammation. The findings suggest that diosmin may regulate angiogenesis, inflammation, and hypoxia-inducible factor pathway in CVD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Anna Danielewicz, Magdalena Wojciak, Ireneusz Sowa, Monika Kusz, Joanna Wessely-Szponder, Slawomir Dresler, Michal Latalski
Summary: This study investigated the concentrations of bone remodeling-related agents in adolescent idiopathic scoliosis (AIS) patients, and found that the levels of vitamin D, N-terminal propeptide of type I procollagen, and fibroblast growth factor-23 were altered in these patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Martyna Zagorska-Dziok, Aleksandra Ziemlewska, Agnieszka Mokrzynska, Zofia Niziol-Lukaszewska, Magdalena Wojciak, Ireneusz Sowa
Summary: This study assessed the biological activity of extracts and hydrogels containing Cornus mas L. extracts. The results showed that these extracts and hydrogels had strong antioxidant properties, positively affected the viability of skin cells, stimulated cell migration, inhibited collagenase and elastase activity, and increased skin hydration.
Article
Chemistry, Physical
Jedrzej Kozak, Katarzyna Tyszczuk-Rotko, Aleksy Keller, Magdalena Wojciak, Ireneusz Sowa
Summary: The purpose of this study was to develop a simple, fast, and sensitive method for determining the polyphenolic antioxidant curcumin. By electrochemically activating a screen-printed boron-doped diamond electrode, the analytical signal of curcumin was significantly amplified. Under optimized conditions, the method achieved a very low detection limit and yielded consistent results compared to other commonly used methods.
Review
Medicine, General & Internal
Jaromir Jarecki, Marcin Krzysztof Wasko, Wojciech Widuchowski, Agnieszka Tomczyk-Warunek, Magdalena Wojciak, Ireneusz Sowa, Tomasz Blicharski
Summary: This review provides an overview of the commonly used clinical methods for treating articular cartilage defects, with a specific emphasis on recent clinical trials. Currently, no single method fully satisfies the requirements for effective cartilage healing while remaining easily applicable during surgical procedures. However, there are several available options and the choice of treatment should take into consideration factors such as the location and size of the cartilage lesion, patient preferences, and whether it is chondral or osteochondral in nature. Promising future directions include tissue engineering, stem cell therapies, and the development of pre-formed scaffolds from hyaline cartilage, offering hope for improved outcomes.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Ireneusz Sowa, Roman Paduch, Jaroslaw Moldoch, Dariusz Szczepanek, Jacek Szkutnik, Pawel Sowa, Katarzyna Tyszczuk-Rotko, Tomasz Blicharski, Magdalena Wojciak
Summary: This study assessed the cytotoxic potential of Carlina vulgaris against human colorectal adenocarcinoma and identified several compounds linked to this effect. One of the compounds exhibited significant cytotoxicity, although without selectivity. The cytotoxicity was associated with the generation of intracellular reactive oxygen species.
Article
Biochemistry & Molecular Biology
Martyna Zagorska-Dziok, Aleksandra Ziemlewska, Agnieszka Mokrzynska, Zofia Niziol-Lukaszewska, Ireneusz Sowa, Dariusz Szczepanek, Magdalena Wojciak
Summary: The properties of extracts and ferments from Cornus mas L. were compared for their potential use in cosmetic products. The study evaluated their composition, antioxidant properties, cytotoxicity, collagenase and elastase inhibitory activity, and antimicrobial properties. Both the extract and ferment showed antioxidant and anti-aging effects, making them promising ingredients in skin care preparations. The stronger activity of the ferment suggested the legitimacy of utilizing kombucha fermentation process for valuable cosmetic products.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Jaromir Jarecki, Bartosz Potoczniak, Artur Dziedzic, Teresa Malecka-Masalska, Tomasz Skrzypek, Waldemar Kazimierczak, Marcin Skowronek, Magdalena Wojciak, Slawomir Dresler, Marcin Wasko, Ireneusz Sowa
Summary: This study assessed the relationship between the severity of knee osteoarthritis (KOA) and body composition in postmenopausal women using bioimpedance analysis (BIA). The results showed that overweight and obesity in postmenopausal women are primarily associated with adipose tissue level and its metabolic activity.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Peripheral Vascular Disease
Marcin Feldo, Magdalena Wojciak, Ireneusz Sowa, Stanislaw Przywara, Pawel Sowa, Roman Paduch
Summary: Diosmin and diosmetin have different impacts on pro-inflammatory factors depending on the inflammation inducer. Diosmetin more effectively modulated inflammation than diosmin, suggesting that the attachment of the sugar moiety attenuates its activity.
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).
