Article
Chemistry, Applied
Jing Ren, Lihong Su, Huilan Hu, Xuechi Yin, Jingke Xu, Sijie Liu, Jianlong Wang, Zhanhui Wang, Daohong Zhang
Summary: A third-stage amplifier indirect probe (TsAIP) based lateral flow immunoassay (LFIA) was developed for detecting furazolidone (FZD) using Prussian blue nanoparticles (PBNPs) as carrier. The method achieved primary, second-stage, and third-stage signal amplification, showing improved detection performance and expanded detection range for FZD metabolite derivative, with successful application in food samples. This approach provides a platform for broadening the detection range and application of PBNPs based LFIAs.
Article
Chemistry, Analytical
Kentaro Ito, Kumi Y. Inoue, Kosuke Ino, Hitoshi Shiku
Summary: In this study, a high-sensitivity dual immunoassay using Lumulus amebocyte lysate (LAL) and blood coagulation cascade reactions with redox cycling in a nanoscale-gap electrode was developed. The dual immunoassay showed high sensitivity for detecting two types of analytes, and the signal amplification by the cascade reactions and the use of redox cycling in a nanoscale gap contributed to the high sensitivity. The cross reaction between the LAL and coagulation cascade reactions was minimized by bisecting the beads after the immunocomplex formation. The dual immunoassay demonstrated its potential for clinical diagnosis requiring the detection of multiple analytes.
ANALYTICAL CHEMISTRY
(2022)
Review
Biotechnology & Applied Microbiology
Yanna Shao, Huan Zhou, Qingping Wu, Yonghua Xiong, Juan Wang, Yu Ding
Summary: This study systematically discusses the importance of enzyme-based signal transduction patterns in immunoassay, as well as different strategies for enhancing the output signals of enzymes to significantly improve the sensitivity of enzyme-based immunosensors. The article categorizes these strategies into three categories and discusses the current focus and future direction of enzyme-based immunoassays.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Chemistry, Analytical
Danhua Wang, Yibei Dai, Xuchu Wang, Pan Yu, Shufang Qu, Zhenping Liu, Ying Cao, Lingyu Zhang, Ying Ping, Weiwei Liu, Zhihua Tao
Summary: The RCA-CLIA method enables accurate quantitation of plasma A beta, showing higher sensitivity and specificity than conventional CLIA. The A beta 42/A beta 40 ratio in plasma serves as an accurate indicator for AD diagnosis, indicating great potential for noninvasive diagnosis and progressive assessment of AD.
Article
Chemistry, Analytical
Yi Li, Fei Deng, Ewa M. Goldys
Summary: The study demonstrates the use of CRISPR/Cas12a ribonucleoproteins to enhance the sensitivity of commercial ELISA systems by amplifying fluorescence signals. The approach, known as CES-Amplifier, involves integrating a hybrid single strand DNA oligo and antibody conjugate targeting the HRP enzyme into commercial ELISA kits, allowing for expansion of CRISPR/Cas-based biosensing to a wide range of non-nucleic acid analytes.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Yi Li, Fei Deng, Ewa M. Goldys
Summary: The study demonstrates the successful integration of CRISPR/Cas12a-based signal amplification into immunoassays using a functional hybrid conjugate of antibody and DNA oligonucleotide. This approach improves the sensitivity of conventional immunoassays, allowing for the detection of small proteins with attomolar sensitivity. The CRISPR-based Universal Immunoassay Signal Enhancer (CRUISE) can be applied to various immunoassays and has the potential to enhance sensitivity and expand the detection range.
Article
Chemistry, Multidisciplinary
Yuhui Chen, Hang Ao, Wencheng Xiao, Huangxian Ju, Jie Wu
Summary: A chemiluminescent imaging assay (CLIA) was developed for the sensitive and convenient detection of SARS-CoV-2 nucleocapsid protein (NP). This assay utilizes a target-induced enzyme activity regulation (T-EAR) strategy to amplify the signal, resulting in a wide detection range and high-throughput imaging detection.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Linzhi Li, Xing Liu, Saijun He, Hongmei Cao, Benchao Su, Tianzeng Huang, Qi Chen, Minghuan Liu, Da-Peng Yang
Summary: A novel ultrasensitive electrochemiluminescence (ECL) immunoassay based on Au/CaCO3 nanocomposites synthesized using waste eggshells was developed for detecting ochratoxin A (OTA) in coffee. The sensor showed high sensitivity, selectivity, low detection limit, excellent performance, and stability, with promising practical applicability in analyzing OTA in food samples. This work demonstrates the potential of utilizing natural waste as a source for fabricating nanomaterials in sensor technology for detecting toxic substances in foods.
Article
Chemistry, Analytical
Jiajia Wang, Wenhui Dong, Xiaomin Yang, Yanan Li, Baokang Jin
Summary: This study developed a highly sensitive electrochemical biosensor based on steric effects for the detection of IgG. The results showed that IgG-modified CdTe quantum dots (CdTe-sig-DNA) limited the hybridization between CdTe-sig-DNA or CdTe-sig-DNA-IgG conjugate and the captured DNA strand (cap-DNA). The concentration of IgG was detected by differential pulse anode stripping voltammetry (DPASV) on a modified glassy carbon electrode surface. A highly sensitive detection of IgG from 5 pM to 50 μM with a low detection limit of 1.7 pM was achieved.
ANALYTICAL METHODS
(2023)
Article
Medicine, General & Internal
Yukihiro Nishikawa, Shiyo Nishida, Keiko Kuroda, Hirokazu Kashiwagi, Yoshiaki Tomiyama, Masataka Kuwana
Summary: Plasma thrombopoietin (TPO) measurements are helpful in distinguishing different types of thrombocytopenia. A newly developed fully automated quantitative chemiluminescent enzyme immunoassay (CLEIA) for TPO measurement showed high accuracy and sensitivity. This assay has the potential to facilitate routine clinical measurement of TPO in patients with various types of thrombocytopenia.
Article
Biochemistry & Molecular Biology
Chao Wang, Songqin Liu, Huangxian Ju
Summary: This article reviews the origin and development of electrochemiluminescent nanoemitters and their application in sensitive detection of protein biomarkers. It specifically focuses on metal-organic frameworks (MOFs)-based nanoemitters and discusses effective strategies to enhance the electrochemiluminescent response and improve immunosensing sensitivity. The article comprehensively presents the application of electrochemiluminescent nanoemitters in the diagnosis of cancers and other diseases, particularly lung cancer and heart diseases. The recent advancement of electrochemiluminescent imaging with nanoemitters for the detection of multiplex protein biomarkers on single cell membranes is also highlighted. Finally, the future opportunities and challenges in the field of electrochemiluminescent biosensing are discussed.
BIOELECTROCHEMISTRY
(2023)
Article
Engineering, Environmental
Feng Hong, Rui Chen, Peng Lu, Letian Li, Ruiheng Xiao, Yiping Chen, Hong Yang
Summary: Inspired by the stability and biocompatibility of polydopamine, a universal, portable, and ultra-sensitive pipet immunoassay platform was developed for the detection of the highly toxic mycotoxin deoxynivalenol (DON). The platform showed high sensitivity, reduced detection cost, and simplified experimental steps.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Medicine, Research & Experimental
Lei Li, Xi Yan, Meng Xia, Bi Shen, Yiting Cao, Xiayu Wu, Jinwen Sun, Yue Zhang, Mingcui Zhang
Summary: The study reveals that nanoparticle formulations have high immunogenicity and antigenicity, enhancing immune responses effectively. The antigenicity of nanoparticle antigens is size-dependent, while nanoparticle drug carriers generate antibody-specific immune responses to their antigens.
MOLECULAR PHARMACEUTICS
(2022)
Review
Chemistry, Analytical
Mingxia Liu, Daiyu Miao, Shaojie Qin, Huwei Liu, Yu Bai
Summary: This review summarizes the comprehensive and essential developments of mass spectrometric immunoassay (MSIA) and its bioanalytical applications. MSIA overcomes the limitation of low ionization efficiency of biomacromolecules through mass tag-based detection, enabling highly sensitive detection and showing great potential in bioanalysis.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Ghasem Anbiaee, Zahra Khoshbin, Hamed Zahraee, Mohammad Ramezani, Mona Alibolandi, Khalil Abnous, Seyed Mohammad Taghdisi
Summary: Developing cost-effective smart sensing tools with ultrahigh sensitivity for food safety insurance is highly important. Aptamer-based biosensors powered by recycling signal amplification strategies, such as target recycling supported by enzymes, have shown great potential in achieving signal amplification. Exonucleases, as superior biocatalysts, simplify the amplification process and exhibit outstanding selectivity and sensitivity, making them promising sensing tools for label-free, ease-of-use, cost-effective, and real-time diagnosis of diverse targets. This article summarizes the achievements and perspectives in aptasensor design for qualitative monitoring of diverse targets using exonucleases for signal amplification.
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).