期刊
TALANTA
卷 83, 期 5, 页码 1415-1421出版社
ELSEVIER
DOI: 10.1016/j.talanta.2010.11.035
关键词
2,3,7,8-Tetrachlorodibenzo-p-dioxin; TCDD; Ah receptor; CALUX; Bioassay; Superinduction
资金
- National Institutes of Environmental Health Sciences (NIEHS) [P42ES004699]
- California Agricultural Experiment Station
- American taxpayers
The chemically activated luciferase expression (CALUX) system is a mechanistically based recombinant luciferase reporter gene cell bioassay used in combination with chemical extraction and clean-up methods for the detection and relative quantitation of 2,3,7,8-tetrachlorodibenzo-p-dioxin and related dioxin-like halogenated aromatic hydrocarbons in a wide variety of sample matrices. While sample extracts containing complex mixtures of chemicals can produce a variety of distinct concentration-dependent luciferase induction responses in CALUX cells, these effects are produced through a common mechanism of action (i.e. the Ah receptor (AhR)) allowing normalization of results and sample potency determination. Here we describe the diversity in CALUX response to PCDD/Fs from sediment and soil extracts and not only report the occurrence of superinduction of the CALUX bioassay, but we describe a mechanistically based approach for normalization of superinduction data that results in a more accurate estimation of the relative potency of such sample extracts. (C) 2010 Elsevier B.V. All rights reserved.
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