期刊
TALANTA
卷 84, 期 3, 页码 607-613出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2010.12.042
关键词
Fe2O3@Au core/shell nanoparticles; Escherichia coli (E. coli); Electrochemical DNA biosensor; Horseradish peroxidase (HRP)
资金
- National Nature Science Foundation of China [20775026]
- Science and Technology Commission of Shanghai Municipality [1052nm06500]
- Shanghai Key Laboratory of Green Chemistry and Chemical Process
A Fe2O3@Au core/shell nanoparticle-based electrochemical DNA biosensor was developed for the ampermetric detection of Escherichia coli (E. coli). Magnetic Fe2O3@Au nanoparticles were prepared by reducing HAuCl4 on the surfaces of Fe2O3 nanoparticles. This DNA biosensor is based on a sandwich detection strategy, which involves capture probe immobilized on magnetic nanoparticles (MNPs), target and reporter probe labeled with horseradish peroxidase (HRP). Once magnetic field was added, these sandwich complexes were magnetically separated and HRP confined at the surfaces of MNPs could catalyze the enzyme substrate and generate electrochemical signals. The biosensor could detect the concentrations upper than 0.01 pM DNA target and upper than 500 cfu/mL of E. coli without any nucleic acid amplification steps. The detection limit could be lowered to 5 cfu/mL of E. coli after 4.0 h of incubation. (C) 2011 Elsevier B.V. All rights reserved.
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