期刊
TALANTA
卷 85, 期 1, 页码 216-221出版社
ELSEVIER
DOI: 10.1016/j.talanta.2011.03.045
关键词
Acetylcholinesterase; Biosensor; Screen-printed electrode; Inhibitor measurement
资金
- RFBR [09-03-92420-KE]
- EINSTEIN Consortium (Italy) [09-03-92420-KE]
A simple and reliable technique has been developed for the construction of an amperometric acetylcholinesterase biosensor based on screen-printed carbon electrodes. For the first time, one-step modification using single-walled carbon nanotubes and Co phtalocyanine has been proposed to decrease the working potential and to increase the signal of thiocholine oxidation. The biosensor developed made it possible to detect 5-50 ppb of paraoxon and 2-50 ppb of malaoxon with detection limits of 3 and 2 ppb, respectively (incubation 15 min). The biosensor showed high reproducibility when measurements of the substrate and inhibitor were performed (R.S.D. about 1% and 2.5%, respectively). The reliability of the inhibition measurements was confirmed by testing spiked samples of sparkling and tape waters. (C) 2011 Elsevier B.V. All rights reserved.
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