Article
Nanoscience & Nanotechnology
Federica Curti, Simone Fortunati, Wolfgang Knoll, Marco Giannetto, Roberto Corradini, Alessandro Bertucci, Maria Careri
Summary: Efficient and timely testing plays a crucial role in managing, controlling, and monitoring the COVID-19 pandemic. This study developed an electrochemical sensing platform based on single-walled carbon nanotube screen-printed electrodes, which can specifically detect the S1 subunit of the SARS-CoV-2 spike protein. The detection is achieved by a concentration-dependent folding of a redox-tagged DNA aptamer, which reduces the electron transfer efficiency and suppresses the amperometric signal.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biochemical Research Methods
Tzi Shien Yeoh, Thean-Hock Tang, Marimuthu Citartan
Summary: Leptospirosis is a potentially life-threatening disease caused by a bacteria called Leptospira. To improve the speed and accuracy of diagnosis, researchers have developed a DNA aptamer called LepDapt-5a that can directly detect the presence of LipL32 protein, a biomarker for Leptospira. The aptamer was successfully tested on three different diagnostic platforms, showing its potential for rapid and accurate detection of Leptospira.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Multidisciplinary Sciences
Alex M. Yoshikawa, Alexandra E. Rangel, Liwei Zheng, Leighton Wan, Linus A. Hein, Amani A. Hariri, Michael Eisenstein, H. Tom Soh
Summary: Aptamer-based molecular switches have various applications such as imaging metabolites, drug delivery, and biomolecule detection. Conventional aptamer selection methods do not produce aptamers with structure-switching functionality, so post-selection modification is necessary. This study presents a screening-based strategy that can convert any aptamer into a molecular switch without prior knowledge of its structure.
NATURE COMMUNICATIONS
(2023)
Article
Biophysics
Shihua Liu, Qiuyan Li, Huili Yang, Po Wang, Xiangmin Miao, Qiumei Feng
Summary: An in situ quenching electrochemiluminescence biosensor sensitized with aptamer recognition-induced multi-DNA release was designed for sensitive detection of pathogenic bacteria, with outstanding specificity and amplification effect, showing potential for infectious food tests.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Multidisciplinary
Velu Ranganathan, Spencer Boisjoli, Maria C. DeRosa
Summary: In this study, a FRET-based fluorescent aptasensor was optimized for the detection of ochratoxin A (OTA) by utilizing the quenching efficiency of single-walled carbon nanotubes (SWCNTs) and the binding affinity of aptamers. The results showed that acid-modified SWCNTs and the A08min aptamer exhibited better detection performance.
Review
Biochemistry & Molecular Biology
Korinn N. Murphy, Amanda J. Brinkworth
Summary: Focal adhesions (FAs) play a crucial role as dynamic signaling hubs within the cell, connecting intracellular actin with the extracellular matrix (ECM) to facilitate processes such as cell-ECM adhesion and migration. Pathogenic microbes often modify host cell actin cytoskeletons in order to enhance uptake and survival.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Multidisciplinary Sciences
Pierre-Marc Delaux, Sebastian Schornack
Summary: Over 450 million years of evolution on land, plants and microbes have formed diverse relationships, from parasitism to mutualism. Scientists have started to unravel the genetic basis and evolutionary trajectories of plant-microbe associations through phylogenetics, cell biology, and reverse genetics. The co-option of protection mechanisms and general cell biology principles is proposed as the basis for symbiosis.
Article
Engineering, Environmental
Stella G. Michael, Barbara Drigo, Irene Michael-Kordatou, Costas Michael, Thomas Jaeger, Samuel C. Aleer, Thomas Schwartz, Erica Donner, Despo Fatta-Kassinos
Summary: This study assessed the effectiveness of ultrafiltration (UF) in removing antibiotic-related microcontaminants from urban wastewater. The results showed that UF combined with granular activated carbon adsorption can effectively remove antibiotics. Furthermore, UF was also able to reduce the load of cultivable bacteria, antibiotic-resistant bacteria, and antibiotic-resistance genes, as well as exhibit toxicity inhibition towards aquatic organisms and plants.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Microbiology
Kylie J. Boyce
Summary: Genetic diversity within microbial populations is crucial for adaptation to changing environmental conditions, which arises from mutations. Mutators, strains with elevated mutation rates, can facilitate emergence of antibiotic resistance and allow phenotypic modifications for survival, but a delicate balance is needed to maintain genomic integrity.
Review
Biochemistry & Molecular Biology
Diandian Li, Yuan Su, Jie Li, Rong Liu, Bing Fang, Jingjing He, Wentao Xu, Longjiao Zhu
Summary: Bacteriostatic aptamers obtained through SELEX screening are a promising strategy for combating bacterial infections. They can reduce bacterial infection through various mechanisms and enhance the function of other antibacterial materials/drugs.
Review
Environmental Sciences
Karthik Basthi Shivaram, Pankaj Bhatt, Mohit S. Verma, Kari Clase, Halis Simsek
Summary: Wastewater from various sources can accumulate pathogenic microorganisms and toxic chemicals when discharged untreated into the environment, leading to waterborne contamination and human deaths. To address this issue, there is a need for faster identification and treatment of harmful microorganisms and substances. Biosensors are devices that can provide quantitative information quickly, and several have been developed for measuring parameters and microorganisms in wastewater. Bacteriophage-based biosensors offer the possibility of identifying specific live bacteria in infected environments. This paper investigates the current state of microbial community analysis and biosensor development for detecting pathogenic microorganisms.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biophysics
Luana Barbosa Correa, Suyene Rocha Pinto, Luciana Magalhaes Rebelo Alencar, Sotiris Missailidis, Elaine Cruz Rosas, Maria das Gracas Muller de Oliveira Henriques, Ralph Santos-Oliveira
Summary: A nanoaptamer anti-MUC1/Y was developed and evaluated as a nanoagent for rheumatoid arthritis treatment, showing high entrapment efficacy and significant therapeutic effects, especially at low doses. The results confirm the applicability of this nanosystem for RA treatment.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Engineering, Environmental
Xunfeng Chen, Juncai Wang, Yimin You, Renyuan Wang, Shaohua Chu, Yaowei Chi, Kashif Hayat, Nan Hui, Xinxin Liu, Dan Zhang, Pei Zhou
Summary: The study demonstrated that the application of MCNT increased soil enzyme activities that promote plant growth, but resulted in reductions in available nitrogen and potassium contents. Although the taxonomic compositions of soil microbes were altered by MCNT, the diversity of bacterial and fungal communities did not significantly change. The study also revealed a correlation between microbial community structure and soil organic carbon content, indicating the strong resistance and adaptation ability of soil microbes to carbon nanotubes in the presence of plants.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Review
Biochemical Research Methods
Yin Jin, Zheng Wang, An-Yu Dong, Yuan-Qin Huang, Ge-Fei Hao, Bao-An Song
Summary: This study investigated online resources related to insecticides, antimicrobials, medicinal plants, and plant essential oils, showcasing their functionalities, data sources, and applicability. The research also discussed limitations of these resources, providing a valuable toolbox for scientists.
BRIEFINGS IN BIOINFORMATICS
(2021)
Editorial Material
Biotechnology & Applied Microbiology
Peter P. Du, Katherine Liu, Michael C. Bassik, Gaelen T. Hess
Summary: CRISPR base- and prime-editing pooled screens are used to reveal the function of genetic variants with unprecedented resolution.
NATURE BIOTECHNOLOGY
(2022)
Review
Chemistry, Analytical
Kyle B. Lynch, Jiangtao Ren, Matthew A. Beckner, Chiyang He, Shaorong Liu
ANALYTICA CHIMICA ACTA
(2019)
Article
Biophysics
Zhipeng Xie, Jinlong Lei, Mingfeng Yang, Youji Li, Xinhua Geng, Simin Liu, Jiahai Wang
BIOSENSORS & BIOELECTRONICS
(2019)
Article
Chemistry, Physical
Qinghe Cao, Chuanqin Wang, Shujie Chen, Xingdong Xu, Fenggang Liu, Xinhua Geng, Jiahai Wang
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2019)
Article
Biochemistry & Molecular Biology
Mengmeng Lv, Yuchun Guo, Jiangtao Ren, Erkang Wang
NUCLEIC ACIDS RESEARCH
(2019)
Article
Materials Science, Multidisciplinary
Yongsheng Dong, Shengzhou Chen, Jialei Liu, Jinlong Lei, Fenggang Liu, Wei Yang, Jiahai Wang
Article
Chemistry, Multidisciplinary
Mengmeng Lv, Weijun Zhou, Daoqing Fan, Yuchun Guo, Xiaoqing Zhu, Jiangtao Ren, Erkang Wang
ADVANCED MATERIALS
(2020)
Article
Chemistry, Inorganic & Nuclear
Yuting Wang, Ting Wang, Rong Zhang, Qian Liu, Yongsong Luo, Guanwei Cui, Siyu Lu, Jiahai Wang, Yongjun Ma, Xuping Sun
INORGANIC CHEMISTRY
(2020)
Article
Chemistry, Multidisciplinary
Yuchun Guo, Mengmeng Lv, Jiangtao Ren, Erkang Wang
Summary: This work demonstrates exquisite engineering of catalytic activity of DNA-templated silver nanoclusters (DNA-AgNCs) through unique adsorption phenomena of DNAs on DNA-AgNCs and reversible transition between double and triple-stranded DNAs. The study shows that the catalytic activity of DNA-AgNCs can be reversibly regulated by controlling the types of polymers and DNA structures.
Article
Electrochemistry
Huanhuan Xing, Hongyin Xia, Yongchao Fan, Yuan Xue, Chao Peng, Jiangtao Ren, Jing Li, Erkang Wang
Summary: Highly conductive MXene-Ti3C2 was employed as nanocarriers to anchor ECL luminophore Ru(bpy)(3)(2+), forming Ti3C2-Ru composite within seconds. The solid-state ECL platform was constructed with the assistance of Nafion, providing good electron transfer capacity and stable loading of Ru(bpy)(3)(2+) for bioanalysis.
Article
Chemistry, Multidisciplinary
Yuchun Guo, Jiangtao Ren, Erkang Wang
Summary: This work presents the exquisite logic manipulation of nanometallic catalysis of DNA-templated silver nanoclusters (DNA-AgNCs) based on designative input-responsive DNA allostery and distinct inhibition effects of duplexes and triplexes on catalytic activity. A series of molecular logic gates are introduced, capable of controlling the catalytic activity of DNA-AgNCs in logic manners. The importance of this study lies in the intelligent detection of significant disease biomarkers using a low-cost and universal platform.
Review
Chemistry, Analytical
Liu He, Yuchun Guo, Yunhui Li, Jianwei Zhu, Jiangtao Ren, Erkang Wang
Summary: Nucleic acid aptamers are important in the field of bioanalysis and can be used to construct aptasensors for detecting various disease biomarkers. These sensors have advantages such as good stability, easy synthesis, and low cost.
JOURNAL OF ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Husile Bai, Liu He, Jing-Hai Liu, Zuo-Jia Liu, Jiang-Tao Ren, Er-Kang Wang
Summary: This work presents a simple enzyme-linked hybrid-sandwich assay (ELHSA) for the detection of cardiac troponin I (cTnI), an important cardiac biomarker. The study designed a unique capture antibody-target detection aptamer configuration, and obtained optimal antibody-aptamer combination and ratio between streptavidin-horseradish peroxidase conjugate and biotin-modified aptamer. The research revealed a beneficial interaction mode between cTnI and the two recognition elements, and discovered that bovine serum albumin can be used as an enhancer in the detection process.
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Yuchun Guo, Jiangtao Ren, Erkang Wang
Summary: This review summarizes the significant progress in DNA-encoded nanomaterials and their controllable properties, and presents the engineering methods based on DNA-metal ion and DNA-nanomaterial interactions. The study reveals the advantages of DNA modules in terms of strong specific interactions, stimulus-responsive features, and addressable functions.
ANALYSIS & SENSING
(2023)
Article
Chemistry, Analytical
Xiushuang Fan, Jinpeng Qiu, Chao Peng, Jiangtao Ren, Huanhuan Xing, Chuyao Bi, Jianyuan Yin, Jing Li
Summary: A novel sensing concept based on boron nitride quantum dots and copper ions is proposed for monitoring dopamine concentration through the in situ formation of polydopamine, showing excellent selectivity and detection limit. The experimental results demonstrate good reproducibility and stability of the proposed sensing system for assessing dopamine in human plasma samples.
Article
Chemistry, Multidisciplinary
Weijun Zhou, Youxing Fang, Jiangtao Ren, Shaojun Dong
CHEMICAL COMMUNICATIONS
(2019)
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).
