Article
Chemistry, Analytical
Pian Wu, Man Zhang, Xiaoting Xue, Ping Ding, Lei Ye
Summary: A novel dual-amplification system based on CRISPR-Cas12a and horseradish peroxidase (HRP) was developed for colorimetric determination of MC-LR. The system combines the nuclease activity of CRISPR-Cas12a with the redox activity of HRP. The dual-signal amplification enables the detection of MC-LR in a range of 0.01 to 50 ng·mL(-1), with a limit of detection of 4.53 pg·mL(-1).
Article
Pharmacology & Pharmacy
Jie Wang, Yan Ren, Xiufen Zheng, Jiaqi Kang, Zhenqian Huang, Lizhi Xu, Yaping Wang
Summary: This study demonstrated the anti-fibrotic effect of microcystin-RR on bleomycin-induced pulmonary fibrosis rodent models, attenuating pulmonary inflammation, collagen deposition, and fibrotic markers expression. Microcystin-RR treatment also reduced the number of pulmonary M2 macrophages and inhibited epithelial-mesenchymal transition and fibroblast-myofibroblast transition triggered by M2 macrophages. These findings suggest that microcystin-RR is a promising candidate for therapeutic and prophylactic strategies against idiopathic pulmonary fibrosis.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Soraia P. S. Fernandes, Petr Kovar, Milan Psenicka, Artur M. S. Silva, Laura M. Salonen, Begona Espina
Summary: This study investigated the adsorption performance of three COFs for different MC derivatives, revealing different adsorption efficiencies for different toxins. By selecting COF functional groups, specific compounds can be selectively targeted or excluded, providing insights for the design of more efficient and selective adsorbent materials.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Environmental Sciences
Sijia Hua, Jiawen Chen, Liang Wu, Xinyue Yu, Jing Ye, Yuanting Li, Yongqiang Zhu, Fuxiang Tian
Summary: This study detected the contents of MC-LR in Microcystis aeruginosa cultures and water samples from the Huangpu River using ELISA and HPLC methods. The results showed that ELISA was a reliable and simple method for monitoring low levels of MC-LR. The concentration of MC-LR in the Huangpu River varied with the season, but remained below the WHO drinking water quality standard.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Chemistry, Analytical
Nobuyuki Futai, Yuto Fukazawa, Tomohiro Kashiwagi, Shogo Tamaki, Riho Sakai, Catherine A. Hogan, Kanagavel Murugesan, Ashwin Ramachandran, Niaz Banaei, Juan G. Santiago
Summary: This study presents a method for the high-efficiency separation and extraction of short cfDNA fragments, using a millimeter-scale fluidic device to extract the fragments into solid agarose gel slabs from liquid human plasma samples. The method demonstrates high sensitivity in cfDNA detection.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Liang Fan, Gang Xiao, Mingliang Wang, Shuang Zhao, Qiaochun Yang, Linyang Cheng, Jinhui Jeanne Huang, Zhao Yue
Summary: By optimizing experimental conditions and introducing different nanomaterials, the sensing properties of PEC immunosensors based on MUA-AuNCs were successfully enhanced. Among them, MUA-AuNCs/cGO showed the best sensing performance.
ANALYTICA CHIMICA ACTA
(2021)
Article
Engineering, Environmental
Yarui Wang, Fengjie Chen, Wanchao Yu, Lixia Zhao
Summary: The newly designed x PDDA-gC3N4-NPTEG material shows excellent ability to attack the toxic foundational groups of MC-LR and has high recycling efficiency, making it a promising method for effectively treating drinking water.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Chemistry, Applied
Zhaomin Wang, Bingyan Li, Ping Xie, Yong Liu
Summary: In this study, a novel probe, Bptp-MR, was described to concurrently report MC-LR and lysosomes. The application of this probe provided insights into the interference of MC-LR with the intracellular acidic region, which is important for studying the pathogenesis of MC-LR-related diseases.
Article
Environmental Sciences
Huixia Zhang, Zhaomin Wang, Yong Liu, Ping Xie
Summary: This study investigated the direct effect of MC-LR on DNA using spectral analysis and molecular biology technology. The results showed that there is a significant direct interaction between MC-LR and DNA, but it does not cause DNA strand breaks, change DNA configuration, or affect DNA replication. The effects of MC-LR on DNA mainly originate from secondary effects.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Food Science & Technology
Xueqiong Yao, Ying Liu, Yue Yang, Yafang Li, Na Hu, Fengmei Song, Fei Yang
Summary: Previous studies have established that MC-LR is a risk factor for kidney damage. However, the exact regulation mechanism of MC-LR and its mitochondria-related mechanism leading to kidney damage remain limited. Therefore, this study aimed to explore the inhibitory effect of MC-LR on mitophagy and its toxicological mechanism in kidney toxicity.
Article
Crystallography
Fiora Artusio, Albert Castellvi, Roberto Pisano, Jose A. Gavira
Summary: Agarose gels were used to study the nucleation of three model proteins under different agarose concentrations using a batch method set-up inside flat capillaries. The study revealed the significant influence of agarose on protein crystallization process and demonstrated the versatility of agarose gels in obtaining precise crystal attributes or exploring different polymorphs.
Article
Engineering, Environmental
Jing Yu, Hui Zhu, Heli Wang, Brian Shutes, Tingting Niu
Summary: The rapid development of agriculture has led to increased release of butachlor into aquatic environments. Butachlor has significant toxic effects on the growth, physiology, and molecular mechanisms of Microcystis aeruginosa, a dominant species causing cyanobacterial blooms. Low concentrations of butachlor showed no significant changes, indicating a high tolerance in Microcystis aeruginosa. However, high concentrations of butachlor resulted in inhibition of photosynthetic activity, disruption of cell ultrastructure, oxidative stress, and elevated extracellular microcystin-LR concentration.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Review
Environmental Sciences
Shiyu Zhang, Xingde Du, Haohao Liu, Michael D. Losiewic, Xinghai Chen, Ya Ma, Rui Wang, Zhihui Tian, Linjia Shi, Hongxiang Guo, Huizhen Zhang
Summary: MC-LR is an emerging environmental pollutant produced by cyanobacteria that poses a threat to wildlife and human health by damaging reproductive organs and causing trans-generational and developmental toxicity. The mechanisms of MC-LR-induced reproductive toxicity mainly include the inhibition of protein phosphatase activity and the induction of oxidative stress, with potential involvement of epigenetic modifications. Additional research is needed to further understand the estrogenic effects of MC-LR and its impact on reproductive health.
ENVIRONMENTAL RESEARCH
(2021)
Article
Chemistry, Analytical
Wei Yue, Bingyong Lin, Yueyue Huang, Yueliang Wang, Yuanyuan Yao, Lifen Chen, Yanbo Zeng, Lei Li, Zhaosheng Qian, Longhua Guo
Summary: A sensing strategy using aptamer-recognized SERS tag and magnetic separation was developed for ultrasensitive detection of MC-LR in fish organs. The results showed that MC-LR content in fish meat exceeded the WHO proposed tolerable daily intake level, indicating potential threats to human health.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Environmental Sciences
Shengquan Zeng, Eunsung Kan
Summary: Novel iron activated biochars (FA-BCs) were prepared by simultaneous pyrolysis and activation of FeCl3-pretreated bermudagrass (BG), showing enhanced surface area, adsorption capacity, and high regeneration efficiency for removing microcystin-LR (MC-LR) in aqueous solution. The possible mechanisms for MC-LR adsorption onto FA-BC include electrostatic attraction, p-π interaction, hydrogen bond, and hydrophobic interactions. The detailed adsorption studies indicated chemisorption and intra-particle diffusion limitation as main factors in the adsorption process, with high efficiency achieved in regeneration and removal of MC-LR from real lake water.
Article
Biochemical Research Methods
Selma Rabai, Messaoud Benounis, Gaelle Catanante, Abdoullatif Baraket, Abdelhamid Errachid, Nicole Jaffrezic Renault, Jean-Louis Marty, Amina Rhouati
Summary: In this study, a new aptasensor for detecting cadmium (Cd2+) in water was developed. The sensor showed a good linear relationship between the Cd2+ concentration and impedance changes, with a high sensitivity and selectivity towards Cd2+ compared to other interferents. The developed aptasensor provides a simple and sensitive approach for trace Cd2+ detection in aqueous solutions.
ANALYTICAL BIOCHEMISTRY
(2021)
Review
Food Science & Technology
Marjan Majdinasab, Sondes Ben Aissa, Jean Louis Marty
Summary: Colorimetric methods show great potential in mycotoxins analysis due to their visual read-out, easy operation, cost-effectiveness, and rapid response.
Article
Chemistry, Analytical
Selma Rabai, Ahlem Teniou, Gaelle Catanante, Messaoud Benounis, Jean-Louis Marty, Amina Rhouati
Summary: A label-free electrochemical aptasensor for cadmium detection in water was developed using a nanocomposite combining gold nanoparticles, carbon nanotubes, and chitosan. The biosensor exhibited excellent performances for cadmium detection with high sensitivity, low detection limit, and a wide linear range. It also showed good selectivity against interfering ions.
Article
Chemistry, Medicinal
Marjan Majdinasab, Mihaela Badea, Jean Louis Marty
Summary: The lateral flow assay (LFA) is a widely used paper-based platform for rapid and on-site detection of various analytes. While antibodies are traditionally used in LFAs, they have limitations such as poor stability and high cost, making aptamers a promising alternative for biorecognition molecules in LFAs.
Article
Chemistry, Analytical
Xiaoqian Tang, Qi Zhang, Maria Isabel Pividori, Zhaowei Zhang, Jean-Louis Marty, Gaelle Catanante
Summary: Patulin contamination is a serious health risk factor for food safety and human health. In this study, a sensitive aptamer-based lateral flow assay was developed for rapid and simple patulin detection in apple juice.
Article
Chemistry, Applied
Xiaoqian Tang, Gaelle Catanante, Xiaorong Huang, Jean-Louis Marty, Hong Wang, Qi Zhang, Peiwu Li
Summary: This study successfully devised a nontoxic electrochemical immunosensor for the quantitative determination of aflatoxin M-1. The immunosensor demonstrated good selectivity and sensitivity, making it suitable for practical applications such as milk samples.
Review
Chemistry, Medicinal
Marjan Majdinasab, Jean Louis Marty
Summary: The early diagnosis of diseases is crucial for effective treatment, and biomarkers offer great potential in this area. Aptamer-based biosensors, with their high sensitivity and selectivity, have emerged as a promising diagnostic tool. Electrochemical aptasensors have attracted significant attention due to their high sensitivity and accuracy in biomarker detection.
Review
Chemistry, Medicinal
Amina Rhouati, Adel Rhouati, Jean Louis Marty
Summary: Detection and quantification of biomarkers in biological fluids are crucial for screening, diagnosing, and treating diseases. Aptamers have recently emerged as an alternative to antibodies for sensing various biomarkers. With different transducers, aptamers offer simple and rapid tools for highly sensitive and selective detection. This review focuses on the use of aptamers in biosensing cardiac biomarkers, specifically Troponin I, B-type natriuretic peptide, and myoglobin. The principles and characteristics of electrochemical aptasensors reported in the literature over the past five years are also discussed.
CURRENT TOPICS IN MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
V. Sunil Kumar, Shekher Kummari, Gaelle Catanante, K. Vengatajalabathy Gobi, Jean Louis Marty, K. Yugender Goud
Summary: A label-free electrochemical impedimetric immunosensor was developed for rapid and sensitive detection of zearalenone (ZEN) using palladium nanoparticle embedded functionalized carbon nanotube-chitosan (SPCE/CS-CNT-Pd) modified electrode. The structure of the nanocomposite was analyzed and verified using X-ray diffraction, transmission electron microscopy, and energy dispersive X-ray spectroscopy. The coexistence of Pd nanoparticles with CNTs enhanced the conductivity and catalytic properties of the transducer surface, as well as improved antibody immobilization. The immunosensor showed high sensitivity and simplicity compared to conventional methods and successfully detected ZEN in corn samples with good recovery values.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Rabia Batool, Sara Riaz, Shehar Bano, Akhtar Hayat, Muhammad Shahid Nazir, Muhammad Nasir, Jean Louis Marty, Mian Hasnain Nawaz
Summary: A flexible CeO2 nanostructured polydopamine-modified carbon cloth (CeO2/PDA/CC) interface was fabricated for ethanol detection. The fabrication method involved electrodeposition of dopamine on carbon fibers followed by electrochemical growth of CeO2 nanoparticles. The CeO2/PDA-based electroactive interface exhibited impressive electrochemical performance due to the synergistic effect of PDA functionalization and active sites. The CeO2 nanostructures anchored on conductive CC contributed to superior electrocatalytic performance. The designed electrochemical sensor showed wide response range, low detection limit, good anti-interference ability, and excellent repeatability and reproducibility, making it suitable for practical implementation in saliva samples.
Review
Chemistry, Analytical
Jagadeeswara Rao Bommi, Shekher Kummari, Kavitha Lakavath, Reshmi A. Sukumaran, Lakshmi R. Panicker, Jean Louis Marty, Kotagiri Yugender Goud
Summary: Cancer is a significant global public health issue, accounting for the second highest number of deaths, especially in low- and middle-income countries. It has a huge economic burden of over a trillion dollars annually. Early detection plays a crucial role in effective treatment and improved survival rates, as a large percentage of cancers are diagnosed at late stages. Biosensors offer promise in the early screening of cancer biomarkers. This review highlights recent findings of novel cancer biomarkers, their advantages over traditional biomarkers, and innovative biosensing and diagnostic methods, aiding in early recognition and treatment monitoring of various human cancers.
Review
Chemistry, Analytical
Marjan Majdinasab, Marc Lamy de la Chapelle, Jean Louis Marty
Summary: Interleukin 6 (IL-6) is a pleiotropic cytokine that exerts pathological pro-inflammatory effects and plays important roles in various biological processes. The detection of IL-6 in small quantities with high selectivity is crucial in medical and biological fields. Conventional laboratory methods such as ELISAs and CLIAs are complex, expensive, and time-consuming, while biosensors offer a simple, rapid, economical, and user-friendly alternative due to their high sensitivity, quick detection time, ease of use, and miniaturization capability.
Article
Chemistry, Analytical
Ahlem Teniou, Ibrahim A. Madi, Riane Mouhoub, Jean Louis Marty, Amina Rhouati
Summary: In this study, a homogeneous technique was developed for sensitive and rapid quantification of hydrogen peroxide using hemoglobin as a bioreceptor. The chemiluminescence reagent luminol served as a peroxidase substrate and generated a chemiluminescent signal when oxidized by hemoglobin. The biosensor demonstrated high selectivity and a detection limit of 0.308 mM, making it a cost-effective and simple method for monitoring peroxidase substrates.
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).