4.7 Article

Evaluation of the effects of Candidatus Liberibacter asiaticus on inoculated citrus plants using laser-induced breakdown spectroscopy (LIBS) and chemometrics tools

期刊

TALANTA
卷 83, 期 2, 页码 351-356

出版社

ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2010.09.021

关键词

Laser-induced breakdown spectroscopy; Chemometrics; Fingerprint; Plant material; Citrus greening; Huanglongbing

资金

  1. Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2007/08618-2]
  2. Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
  3. Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [578576/2008-2]

向作者/读者索取更多资源

This study investigated the organic and inorganic constituents of healthy leaves and Candidatus Liberibacter asiaticus (CLas)-inoculated leaves of citrus plants. The bacteria CLas are one of the causal agents of citrus greening (or Huanglongbing) and its effect on citrus leaves was investigated using laser-induced breakdown spectroscopy (LIBS) combined with chemometrics. The information obtained from the LIBS spectra profiles with chemometrics analysis was promising for the construction of predictive models to identify healthy and infected plants. The major, macro- and microconstituents were relevant for differentiation of the sample conditions. The models were then applied to different inoculation times (from 1 to 8 months). The models were effective in the classification of 82-97% of the diseased samples with a 95% significance level. The novelty of this method was in the fingerprinting of healthy and diseased plants based on their organic and inorganic contents. (C) 2010 Elsevier B.V. All rights reserved.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

Article Food Science & Technology

Impurities in Raw Sugarcane Before and After Biorefinery Processing

Matheus Lima de Mello, Nathalia Zanetti Barros, Marco Aurelio Speranca, Fabiola Manhas Verbi Pereira

Summary: This review examines the impurity issues in sugarcane processing from 1993 to 2021, highlighting the need for specialized analytical methods to address the inherent impurities in cane sugar manufacturing. Various options have been considered to tackle these impurity challenges.

FOOD ANALYTICAL METHODS (2022)

Article Biotechnology & Applied Microbiology

XRF-based analytical methods for supporting sustainability in sugarcane biorefineries

Marco Aurelio Speranca, Paloma Andrade Martins Nascimento, Alejandro Cesar Olivieri, Fabiola Manhas Verbi Pereira

Summary: This study focused on analytical methods for evaluating impurities in sugarcane biorefineries, particularly mineral content, using X-ray fluorescence techniques and multivariate models to discriminate different clarification stages of sugarcane juices. The developed models showed promising results in estimating the contents of various elements with relative prediction errors of <14% and coefficients of determination >0.9083.

BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR (2022)

Article Agriculture, Multidisciplinary

Color approach to the analysis of white crystal cane sugar for the detection of solid impurities

Nathalia Zanetti Barros, Marco Aurelio Speranca, Fabiola Manhas Verbi Pereira

Summary: The study used a digital image system and multivariate calibration strategy to analyze 64 samples of white crystal cane sugar, showing that the method can accurately estimate sugar content and perform well in identifying different content ranges. The new method is affordable and reliable, allowing for quick screening in situations where access to a laboratory and instrumentation is limited.

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2022)

Article Food Science & Technology

Mineral Scale Formed During the Evaporation in Biorefineries - Characterization Using X-ray Fluorescence and Exploratory Analysis

Matheus Lima de Mello, Marco Aurelio Speranca, Fabiola Manhas Verbi Pereira

Summary: This study used energy-dispersive X-ray fluorescence (EDXRF) to analyze samples from sugarcane and corn factories and identified differences in the chemical compositions of mineral scales formed during evaporation. The results can help the factories choose appropriate cleaning methods.

FOOD ANALYTICAL METHODS (2022)

Article Microbiology

A Novel Lineage of Cile-Like Viruses Discloses the Phylogenetic Continuum Across the Family Kitaviridae

Pedro L. Ramos-Gonzalez, Camila Chabi-Jesus, Aline D. Tassi, Renata Faier Calegario, Ricardo Harakava, Claudia F. Nome, Elliot W. Kitajima, Juliana Freitas-Astua

Summary: In this study, molecular and biological features of three new viruses of the family Kitaviridae were described through various analyses. These viruses are transmitted by Brevipalpus mites, which infect plants. The study also identified the Brevipalpus mite species associated with these viruses.

FRONTIERS IN MICROBIOLOGY (2022)

Letter Chemistry, Analytical

Chemometrics Reveals Not-so-Obvious Analytical Information

Fabiola Manhas Verbi Pereira

BRAZILIAN JOURNAL OF ANALYTICAL CHEMISTRY (2022)

Article Biotechnology & Applied Microbiology

Transgenic 'Hamlin' sweet orange expressing csd1 or d4e1 genes exhibits decreased susceptibility to citrus canker disease

Matheus Luis Docema, Tatiana de Souza Moraes, Lisia Borges Attilio, Joao Paulo Rodrigues Marques, Liliane Cristina Liborio Stipp, Ricardo Harakava, Juliana Freitas-Astua, Jose Belasque Junior, Ricardo Antunes Azevedo, Salete Aparecida Gaziola, Francisco de Assis Alves Mourao Filho

Summary: Currently, there are no resistant sweet orange cultivars to citrus canker. Transgenic plants expressing antimicrobial peptides and resistance genes from tolerant cultivars were evaluated for resistance to Xanthomonas citri subsp. citri (Xcc). Transgenic plants showed reduced incidence and severity of citrus canker, as well as lower Xcc titers. Higher superoxide dismutase activity in transgenic plants led to increased hydrogen peroxide production. Callose deposition, an important plant defense response, was observed in transgenic plants with lower Xcc populations.

PLANT CELL TISSUE AND ORGAN CULTURE (2023)

Review Plant Sciences

Kitaviruses: A Window to Atypical Plant Viruses Causing Nonsystemic Diseases

Pedro Luis Ramos-Gonzalez, Gabriella Dias Arena, Aline Daniele Tassi, Camila Chabi-Jesus, Elliot Watanabe Kitajima, Juliana Freitas-Astua

Summary: Kitaviridae is a family of plant-infecting viruses that have unique localized infections and show deficient movement. They are transmitted by mites and infect a wide range of host plants, causing economic damage to crops.

ANNUAL REVIEW OF PHYTOPATHOLOGY (2023)

Article Chemistry, Multidisciplinary

Chemical variability and evaluation of physical parameters of the essential oil of the leaves of Casearia sylvestris varieties and morphoanatomical characterization of the leaves

Flavio A. Carvalho, Fernando B. Oda, Luis V. S. Sacramento, Fabiola M. V. Pereira, Eduardo J. Crevelin, Antonio E. M. Crotti, Andre G. Santos

Summary: This study evaluated the chemical composition and variability of the essential oil (EO) of Casearia sylvestris leaves. Variations in chemical components were found among different varieties and within the same variety. The main compounds in the EO were beta-elemene, alpha-humulene, germacrene D, bicyclogermacrene, spathulenol, caryophyllene oxide, and humulene epoxide II. Additionally, different varieties of C. sylvestris exhibited variations in trunk fissures and leaf morphology.

CHEMICAL PAPERS (2023)

Article Food Science & Technology

Color Classification for Red Alcohol Vinegar to Control the Quality of the End-Product

Giovanna Nalhiati, Gabriel Goncalves Borges, Marco Aurelio Speranca, Fabiola Manhas Verbi Pereira

Summary: This study presents a feasible analytical method for quality control of red alcohol vinegar without the need for dilutions, filtration, or expensive instrumentation. The method eliminates the requirement for adding chemicals. A total of 30 sample solutions were prepared using a 2(2) central composite design (CCD) to produce vinegar with good quality. Analyzing the data from square images converted into color histograms, multivariate classification models were calculated, and they exhibited a reliability higher than 0.9 for accuracy, sensitivity, and specificity. This low-cost and user-friendly analytical method can be useful in identifying operational errors and out-of-specification end-products.

FOOD ANALYTICAL METHODS (2023)

Article Biotechnology & Applied Microbiology

Genetic transformation of 'Hamlin' and 'Valencia' sweet orange plants expressing the cry11A gene of Bacillus thuringiensis as an additional tool for the management of Diaphorina citri (Hemiptera: Liviidae)

Silvia de Oliveira Dorta, Lisia Borges Attilio, Odimar Zanuzo Zanardi, Joao Roberto Spotti Lopes, Marcos Antonio Machado, Juliana Freitas-Astua

Summary: The Asian citrus psyllid (ACP) is the vector of the devastating disease huanglongbing (HLB) in citrus. Previous research has shown the potential of using Bt strains containing cry/cyt genes to control ACP nymphs. This study attempted to produce transgenic sweet orange plants expressing the cry11A gene from Bt, and confirmed the presence and copy number of the transgene using PCR and Southern blot analyses. Greenhouse mortality assays showed that the tested events had effects ranging from 22% to 43% on D. citri nymphs after a five-day exposure period. This is the first report of citrus plants expressing the Bt cry11A gene for the management of D. citri nymphs.

JOURNAL OF BIOTECHNOLOGY (2023)

Article Plant Sciences

Citrus Bright Spot Virus: A New Dichorhavirus, Transmitted by Brevipalpus azores, Causing Citrus Leprosis Disease in Brazil

Camila Chabi-Jesus, Pedro Luis Ramos-Gonzalez, Aline Daniele Tassi, Laura Rossetto Pereira, Marines Bastianel, Douglas Lau, Maria Cristina Canale, Ricardo Harakava, Valdenice Moreira Novelli, Elliot Watanabe Kitajima, Juliana Freitas-Astua

Summary: Citrus leprosis (CL) is a viral disease that seriously affects Brazilian citriculture. In this study, a new virus called citrus bright spot virus (CiBSV) was discovered in sweet orange trees affected by CL in small orchards in Southern Brazil. Sequence analysis revealed that CiBSV belongs to the genus Dichorhavirus and clusters with citrus leprosis virus N, which is transmitted by Brevipalpus phoenicis. Brevipalpus papayensis and B. azores were found in CiBSV-infected citrus plants, with B. azores being the only vector capable of transmitting the virus to Arabidopsis plants.

PLANTS-BASEL (2023)

Article Chemistry, Multidisciplinary

PRINCIPAL COMPONENT ANALYSIS (PCA) PARA A AVALIAcAO DE DADOS QUiMICOS E GERAcAO DE HEAT MAPS: UM TUTORIAL

Dennis da Silva Ferreira, Leticia da Silva Rodrigues, Fabiola Manhas Verbi Pereira, Edenir Rodrigues Pereira-Filho

Summary: This tutorial provides a step-by-step guide on using principal component analysis (PCA) to handle big datasets. Examples of chemical elements' concentration, emission spectrum, and energy-dispersive X-ray fluorescence (EDXRF) of e-waste were used. Five routines were proposed for data processing and PCA calculation, focusing on laser-induced breakdown spectroscopy (LIBS), EDXRF, and heat maps preparation. The tutorial can be implemented in various softwares like MatLab, Octave, R, and Python. Three examples of applying PCA were given, including concentrations and spectra, as well as an example of creating heat maps for a hyperspectral image. A YouTube playlist was also created for further reference. The authors aim to contribute to research in this field.

QUIMICA NOVA (2023)

Article Plant Sciences

A TaqMan RT-qPCR assay for absolute quantification of citrus leprosis virus C lineage SJP: disclosing the subgenomic/genomic ratio in plant and mite vector, plant organ-specific viral loads, and the kinetics of viral accumulation in plants

Gabriella D. Arena, Pedro L. Ramos-Gonzalez, Aline D. Tassi, Marcos A. Machado, Juliana Freitas-Astua

Summary: This study developed two TaqMan RT-qPCR assays for detecting and quantifying Citrus leprosis virus C (CiLV-C) lineage SJP, which is prevalent in the Brazilian citrus belt and the world's main sweet orange production area. The assays targeted two specific genes, p29 and RdRp, and were proven to be efficient and sensitive. By quantifying the target genes in different tissues and organisms, the study revealed the distribution and accumulation of CiLV-C molecules, the higher quantities in sweet orange fruits compared to stems and leaves, as well as the progressive increase during the early stages of infection. These findings contribute to a better understanding of the epidemiology and virus-vector interaction of citrus leprosis.

TROPICAL PLANT PATHOLOGY (2023)

Article Plant Sciences

Expression of the Citrus sinensis EDS5 gene, MATE family, in Solanum lycopersicum L. cv. Micro-Tom enhances resistance to tomato spot disease

Jessika Angelotti-Mendonca, Perla Novais de Oliveira, Nathalia Felipe Ansante, Liliane Cristina Liborio Stipp, Juliana Freitas-Astua, Fernando Manuel Matias Hurtado, Jose Belasque, Francisco de Assis Alves Mourao Filho

Summary: Researchers investigated the role of the CsEDS5 gene in the resistance of transgenic plants by transforming it into Micro-Tom tomato plants. They found that some transgenic plants exhibited higher resistance to X. vesicatoria, which could be significant in mitigating the impact of citrus canker.

TROPICAL PLANT PATHOLOGY (2022)

Article Chemistry, Analytical

Enhancing electrochemiluminescence by modifying Fe3CuO4 and CdS@ZnS: A novel ECL sensor for highly sensitive detection of permethrin

Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu

Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.

TALANTA (2024)

Article Chemistry, Analytical

Ag+-stabilized DNA triplex coupled with catalytic hairpin assembly and CRISPR/Cas12a amplifications for sensitive metallothionein assay

Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang

Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.

TALANTA (2024)

Article Chemistry, Analytical

Boron quantification using ion chromatography tandem triple quadrupole mass spectrometry. Application to retention analysis in boron-treated wood

Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull

Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.

TALANTA (2024)

Article Chemistry, Analytical

Innovative SALDI mass spectrometry analysis for Alzheimer's disease synthetic peptides detection

Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich

Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.

TALANTA (2024)

Article Chemistry, Analytical

Deep eutectic solvents for the determination of endocrine disrupting chemicals

Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere

Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.

TALANTA (2024)

Article Chemistry, Analytical

Nanopore-related cellular death through cytoskeleton depolymerization by drug-induced ROS

Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang

Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.

TALANTA (2024)

Article Chemistry, Analytical

CRISPR-empowered electrochemical biosensor for target amplification-free and sensitive detection of miRNA

Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang

Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.

TALANTA (2024)

Article Chemistry, Analytical

Application and evaluation of molecular docking for aptamer and small molecular interaction- A case study with tetracycline antibiotics

Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong

Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.

TALANTA (2024)

Article Chemistry, Analytical

Highly bright stable organic radicals encapsulated by amphiphilic polypeptide for efficient near-infrared phototheranostics

Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan

Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.

TALANTA (2024)

Article Chemistry, Analytical

The construction of highly selective surface molecularly imprinted polymers based on Cu(II) coordination for the detection of bisphenol A

Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song

Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.

TALANTA (2024)

Article Chemistry, Analytical

A simple joint detection platform for high-throughput single-cell heterogeneity screening

Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu

Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.

TALANTA (2024)

Article Chemistry, Analytical

Nanotechnology-based analytical techniques for the detection of contaminants in aquatic products

Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li

Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.

TALANTA (2024)

Article Chemistry, Analytical

A sensitive off-on electrochemiluminescence DNA sensor based on signal cascade amplification circuit and distance-dependent energy transfer

Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang

Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.

TALANTA (2024)

Article Chemistry, Analytical

A binary system based DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells

Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia

Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.

TALANTA (2024)

Article Chemistry, Analytical

Design and synthesis of hierarchical MnO-Fe3O4@C/expanded graphite composite for sensitive electrochemical detection of bisphenol A

Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang

Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).

TALANTA (2024)