Article
Chemistry, Analytical
M. C. Villegas-Alvarez, A. Arias-Borrego, I. Velasco, T. Garcia-Barrera
Summary: Sample treatment is a bottleneck in untargeted metabolomics, especially for complex samples like food or biofluids. This study developed a new analytical extraction method called three-phase-UA-HF-LPME, which improved the extraction efficiency and was validated using human milk. The method showed better sensitivity, enrichment factors, and metabolite coverage compared to the conventional liquid-liquid extraction method.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Leyla Haidari-Khoshkelat, Jahan Bakhsh Raoof, Milad Ghani, Reza Ojani
Summary: In this study, a combination of two extraction techniques was used to analyze antibiotics in water and urine samples. The method was found to accurately quantify the selected analytes and was applicable to complex biological samples.
ANALYTICA CHIMICA ACTA
(2022)
Review
Chemistry, Analytical
Reham E. Kannouma, Mohamed A. Hammad, Amira H. Kamal, Fotouh R. Mansour
Summary: Liquid-phase microextraction (LPME) is an important technique for sample preparation that is widely used to extract various organic, inorganic, and pharmaceutical compounds from different environmental and biological matrices. LPME has three configurations - single drop microextraction, hollow-fiber liquid-phase microextraction, and dispersive liquid-liquid microextraction - each with different applications and advantages.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Physical
Reza Hoseinpour Kouhestany, Atefeh Tamaddon, Homayon Ahmad Panahi, Arash Afshar Ebrahimi, Rahebeh Amiri
Summary: Researchers synthesized novel hyperbranched dendrimers grafted on SiO2 nanoparticles as nanosorbents for the trace extraction of exemestane. They optimized the parameters for adsorption efficiency and achieved a lower detection limit by using the HF-LPME system. The proposed method, combined with high-performance liquid chromatography, successfully determined exemestane in biological and pharmaceutical samples.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Biochemical Research Methods
Mohammed Altahir Suliman, Taofiq Abdulraheem Olarewaju, Chanbasha Basheer, Hian Kee Lee
Summary: Petroleum is a complex material containing a wide range of compounds, and ASTM methods are globally accepted but have limitations in terms of sample preparation. The challenge in petrochemical analysis is to develop rapid and simpler automated sample preparation procedures. Microextraction is considered an excellent complement to current methods.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemical Research Methods
Jie Bai, Li-yuan Xie, Li Yang, Run-qin Wang, Xuan Chen, Shuang Hu
Summary: A novel hollow fiber liquid phase microextraction method using reversed lipid micelle was developed for the determination of rotigotine in biological samples. Through optimization and validation, the method showed good accuracy, precision, and feasibility in pharmacokinetic studies.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Chemistry, Analytical
Rafael Oliveira Martins, Gabriela Guimaraes Souza, Lucas Santos Machado, Giovanna Lopes de Araujo, Rosineide Costa Simas, Bruno Jose Goncalves da Silva, Virginia Damin, Andrea Rodrigues Chaves
Summary: This study developed a hollow fiber liquid-phase microextraction (HF-LPME) with liquid chromatography-mass spectrometry method (LC/MS) for the extraction and detection of 13 multiclass pesticides from soil samples. The method showed high analytical method response, good linearity, and determined limits of detection and quantification values. It demonstrated an eco-friendlier alternative for analyzing soil samples and has great potential for multiresidue evaluation in environmental samples.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Behrouz Seyfinejad, Kimiya Jouyban, Ahmad Reza Dehpour, Razieh Mohammad Jafari, Mohammad Charkhpour, Mohammad Reza Afshar Mogaddam, Abolghasem Jouyban
Summary: The development of an ion-pair hollow fiber liquid-phase microextraction (IP-HF-LPME) procedure combined with capillary electrophoresis (CE) and indirect UV detection for the simultaneous extraction and determination of aliphatic biogenic amines in rat tissue samples was described. The CE separation and indirect UV detection conditions, as well as the IP-HF-LPME extraction conditions, were studied and optimized. The method demonstrated good performance in terms of linearity, recovery, sensitivity, and repeatability. The detection limits ranged from 2.8 to 4.5 ng mL-1, with a quantification limit of 15 ng mL-1 and a dynamic range of 15-2000 ng mL-1.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Review
Chemistry, Analytical
Ali Pourali, Aysa Abbasalizadeh, Mohammad Reza Afshar Mogaddam, Mir Ali Farajzadeh, Mustafa Tuzen, Mahboob Nemati
Summary: Liquid phase microextraction techniques are a miniaturized version of traditional liquid-liquid extraction, using only a small amount of solvent to extract analytes from a sample. They have been widely applied in the analysis of various analytes in food, pharmaceuticals, and beverages. This review focuses on the application of these techniques in dairy products and discusses the advantages and disadvantages of different liquid phase microextraction methods.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Vilma del Carmen Salvatierra-Stamp, Silvia G. Ceballos-Magana, Norma Susana Pano-Farias, Jose Belisario Leyva-Morales, Kayim Pineda-Urbina, Roberto Muniz-Valencia
Summary: The developed method offers a highly efficient way to quantify multiple emerging contaminants in water samples, with good quantitative results and high enrichment factors in a short total chromatographic time of 5.5 minutes.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Nerea Lorenzo-Parodi, Wiebke Kaziur-Cegla, Astrid Gjelstad, Torsten C. Schmidt
Summary: Aromatic amines, such as those found in tobacco smoke, can enter the human body and be excreted in urine. Hollow fiber-liquid-phase microextraction (HF-LPME) and parallel artificial liquid membrane extraction (PALME) were optimized as alternatives to traditional liquid-liquid extraction (LLE) for measuring these compounds. PALME showed significantly higher recovery rates compared to HF-LPME and was further validated for nine different aromatic amines. The results demonstrate that PALME is a greener, more efficient, and automated alternative to LLE.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Blanka Mikova, Milos Dvorak, Lenka Rysava, Zdenka Mala, Petr Gebauer, Pavel Kuban
Summary: Direct analysis of complex samples is achieved by coupling HF-LPME with CE, demonstrating excellent repeatability and sensitivity. The use of 3D-printed devices ensures stability and reproducibility of the analysis. Coupling HF-LPME with ITP-ESI-MS further enhances selectivity and sensitivity of the analytical method.
Article
Chemistry, Multidisciplinary
John M. Kokosa
Summary: Liquid phase microextraction (LPME) techniques are increasingly important in bioanalytical sample preparation due to their versatility, adaptability, sensitivity, and adherence to green analytical chemistry principles. Continuous innovation and improvements in LPME methods provide potential for new applications in bioanalytical sample preparation. Current trends focus on bioanalytical sample preparation and the use of non-traditional green solvents in LPME methods.
SUSTAINABLE CHEMISTRY AND PHARMACY
(2021)
Review
Engineering, Environmental
Ayo Olasupo, Faiz Bukhari Mohd Suah
Summary: Low concentrations of pharmaceutically active compounds in complex aqueous environments require efficient sample pretreatment methods. Hollow fibre liquid phase microextraction (HF-LPME) is an effective alternative to conventional techniques, consuming less organic solvent and having a shorter extraction time. However, membrane instability remains a limitation. Hollow fibre polymer inclusion membrane liquid-phase micro extraction (HF-PIM-LPME) using ionic liquids shows promise for overcoming this limitation, with high membrane stability and similar selectivity and reproducibility to HF-SLM-LPME.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Environmental Sciences
Zhao-hui Wang, Dou-dou Xu, Xiao-hong Bai, Shuang Hu, Rong-rong Xing, Xuan Chen
Summary: By using charge-transfer supramolecular synergistic three-phase microextraction system, the extraction and enrichment of three nitrophenol isomers were investigated. A combination of charge transfer supramolecular-mediated hollow fiber liquid-phase microextraction (CTSM-HF-LPME) and high-performance liquid chromatography-ultraviolet detector (HPLC-UV) method was established for the determination of real environmental water samples. The study found that the nitrophenols formed charge-transfer supramolecules with electron-rich hollow fibers, promoting their transport in the three-phase extraction system and significantly increasing their enrichment factors. Factors affecting the enrichment factors of the nitrophenols were investigated and optimized, leading to optimal conditions for analysis. The method showed good selectivity and sensitivity, and was successfully applied to the trace detection of real water samples.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Review
Food Science & Technology
Araceli Rivera-Perez, Roberto Romero-Gonzalez, Antonia Garrido Frenich
Summary: This review examines the extraction and clean-up procedures for toxic compounds in spices, herbs, and tea from 2010 to 2020. Analytical methods, including chromatographic separation and mass spectrometry detection, are discussed. The occurrence of these compounds in different matrices is also summarized.
FOOD REVIEWS INTERNATIONAL
(2023)
Article
Biochemistry & Molecular Biology
Julia Baranzelli, Sabrina Somacal, Camila Sant'Anna Monteiro, Renius de Oliveira Mello, Eliseu Rodrigues, Osmar Damian Prestes, Rosalia Lopez-Ruiz, Antonia Garrido Frenich, Roberto Romero-Gonzalez, Martha Zavariz de Miranda, Tatiana Emanuelli
Summary: Pre-harvest sprouting in wheat can be simulated in the laboratory, and different wheat cultivars have distinct behavior in terms of changes in phytochemical compounds during germination. Hard wheat cultivars have higher contents of benzoxazinoids and flavonoids compared to soft wheat cultivars. The matrix-bound phytochemicals decrease only in hard-texture wheat cultivars during germination, due to decreased levels of phenolic acids and flavonoids that are bound to the cell wall. Germination also increases the content of benzoxazinoids and antioxidant capacity in wheat, enhancing its health benefits.
Review
Chemistry, Analytical
Jesus Marin-Saez, Rosalia Lopez-Ruiz, Madalena Sobral, Roberto Romero-Gonzalez, Antonia Garrido Frenich, Isabel M. P. L. V. O. Ferreira
Summary: This study conducted a systematic review of literature from January 1, 2000 to March 15, 2022, focusing on studies related to the analysis of organic chemical hazards in saliva. The review aimed to provide insights into saliva sample collection, extraction, and analytical techniques, as well as the presence of identified compounds. The findings identified significant gaps in the standardized procedures for saliva sampling/collection/storage, limiting the wider use of saliva as a valuable specimen for human biomonitoring of exposure to food chain contaminants.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Food Science & Technology
Maria Elena Hergueta-Castillo, Rosalia Lopez-Ruiz, Antonia Garrido Frenich, Roberto Romero-Gonzalez
Summary: This study evaluated the metabolism and dissipation of flutriafol in different conditions and found that it had a short half-life of less than 17 days. Seven metabolites were detected at low concentrations, and some of them were found to be more toxic than the parent compound.
Article
Chemistry, Applied
Araceli Rivera-Perez, Pascual Garcia-Perez, Roberto Romero-Gonzalez, Antonia Garrido Frenich, Luigi Lucini
Summary: A comprehensive UHPLC-QTOF-HRMS fingerprinting approach was used to trace thyme from three different production regions and evaluate the metabolic differences in response to processing. 37 key compounds related to origin and 33 key compounds related to processing differentiation were identified. The study highlights the terroir influence on thyme composition, with flavonoids, amino acids, and peptides being the most discriminant chemical classes. Thyme sterilization was found to enrich the overall metabolite content, potentially due to increased compound accessibility.
Article
Agriculture, Multidisciplinary
Jesus Marin-Saez, Rosalia Lopez-Ruiz, Roberto Romero-Gonzalez, Antonia Garrido Frenich
Summary: In this study, a method involving solid-liquid extraction and liquid chromatography-high-resolution mass spectrometry was developed to investigate the dissipation of neem oil when applied alone or together with Bacillus thuringiensis (Bt). The dissipation of azadirachtin A (AzA) was found to be faster when neem oil and Bt were applied together at 22 degrees C compared to when neem oil was applied alone at 3 degrees C. Several related compounds and unknown metabolites were also identified in the degraded samples.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Antonio Jesus Maldonado-Reina, Rosalia Lopez-Ruiz, Jesus Marin Saez, Roberto Romero-Gonzalez, Patricia Marin-Membrive, Antonia Garrido-Frenich
Summary: The dissipation of chlorantraniliprole insecticide in tomatoes treated with Altacor 35 WG was studied in laboratory and greenhouse conditions, and suspect screening analysis was performed to identify transformation products and coformulants. Analyses using UHPLC-Q-Orbitrap-MS and GC-Q-Orbitrap-MS showed that chlorantraniliprole followed a biphasic kinetic model with R2 values greater than 0.99. Dissipation was faster in greenhouse studies, with 96% achieved over 53 days. One transformation product (IN-F6L99) was tentatively identified in both laboratory and greenhouse studies, and 15 volatile coformulants were identified by GC-Q-Orbitrap-MS.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemical Research Methods
Ana M. Jimenez-Carvelo, Rosalia Lopez-Ruiz, Roberto Romero-Gonzalez, Antonia Garrido-Frenich
Summary: The study shows that employing an experimental design tool facilitates rapid optimization of critical parameters in mass spectrometry. It also highlights the disadvantage of using commercial databases in compound identification and the superiority of home-made databases.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Beatriz Martin-Garcia, Roberto Romero-Gonzalez, Jose Luis Martinez Vidal, Antonia Garrido Frenich
Summary: A stationary reversed phase based on polyhydroxy methacrylate was used to determine coformulants in 20 plant protection products (PPPs) by liquid chromatography coupled to Q-Orbitrap high-resolution mass spectrometry. A total of 92 coformulants were tentatively identified, with 12 of them quantified by analytical standards. The study found that the polyhydroxy methacrylate-based stationary phase increased the identification of new coformulants in PPPs, complementing conventional C18. This strategy could be applied in future studies to estimate potential coformulant residues from PPPs applied to crops.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Jesus Marin-Saez, Rosalia Lopez-Ruiz, Roberto Romero-Gonzalez, Antonia Garrido Frenich
Summary: This study conducted targeted and nontargeted analysis to investigate the fate of additives in tomato samples treated with two pesticides under greenhouse conditions. Most of the compounds detected degraded within 7 days after the second application.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Review
Chemistry, Analytical
Rosalia Lopez-Ruiz, Jesus Marin-Saez, Osmar D. Prestes, Roberto Romero-Gonzalez, Antonia Garrido Frenich
Summary: This paper discusses the methods for controlling anthropogenic contaminants in edible oils, including sample preparation, analysis, and regulations. Liquid-liquid extraction and QuEChERS methods are commonly used for extraction and analysis, with mass spectrometry being the most commonly used analyzer. The results show that a portion of the analyzed samples exceed the legal limits for pesticides, MCPDs, and PAHs.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).
