Article
Chemistry, Analytical
Tamara Fernandez-Bautista, Beatriz Gomez-Gomez, Roberto Palacin-Garcia, Emma Gracia-Lor, Teresa Perez-Corona, Yolanda Madrid
Summary: The distribution of Se and Hg in different protein fractions extracted from muscle tissues of tuna, swordfish, and salmon (wild vs. farmed) was investigated for the first time. The study revealed that Se and Hg were associated with proteins of specific molecular weights, with differences observed between farmed and wild salmon. Additionally, Se speciation studies showed SeMeSeCys as the primary selenium species found in the proteins analyzed.
Article
Environmental Sciences
Miyuki Iwai-Shimada, Yayoi Kobayashi, Tomohiko Isobe, Shoji E. Nakayama, Makiko Sekiyama, Yu Taniguchi, Shin Yamazaki, Takehiro Michikawa, Masako Oda, Hiroshi Mitsubuchi, Masafumi Sanefuji, Shouichi Ohga, Nathan Mise, Akihiko Ikegami, Reiko Suga, Masayuki Shimono
Summary: Prenatal exposure to methylmercury can impact child development. This study compared two high-throughput methods for mercury speciation and found relatively good agreement between them. MeHg and IHg concentrations were determined in maternal and cord blood samples, highlighting the importance of analyzing different chemical forms for health effects.
Article
Chemistry, Analytical
Yihuan Song, Qingfang Ma, Heyong Cheng, Jinhua Liu, Yuanchao Wang
Summary: A method utilizing online solid-phase extraction coupled with high performance liquid chromatography and inductively coupled plasma mass spectrometry was developed for simultaneous enrichment and analysis of ultra-trace lead and mercury species in water. This method allows for efficient and accurate determination of lead and mercury species in water samples, with high enrichment factors and low LODs and LOQs. The method was successfully applied to analyze trace lead and mercury species in various types of water samples, showing promising results for unstable sample analysis.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Davide Spanu, Laura Butti, Ginevra Boldrocchi, Roberta Bettinetti, Sandro Recchia, Damiano Monticelli
Summary: A fast and cost-effective procedure based on ICP-MS was proposed for the selective determination of methylmercury in fish tissue. The procedure achieved selectivity for methylmercury by inserting a strong anion exchange resin to block inorganic mercury. It featured a short extraction time and analysis time with a low limit of detection. The procedure was validated using standard reference materials and successfully applied to detecting methylmercury in the marine trophic web of Djibouti, revealing a high risk associated with biomagnification of methylmercury.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemical Research Methods
M. Ribeiro, E. Galli, T. Guerin, J. A. L. Silva, I. Castanheira, A. Leufroy, P. Jitaru
Summary: This study presents the development and validation of a new analytical method for simultaneous speciation analysis of selenium and mercury in fish muscle. The method involves the extraction and separation of the different species using microwave-assisted enzymatic hydrolysis and HPLC with a specific column and mobile phase. The method was validated and successfully applied to analyze muscle samples from various fish species.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Applied
Alongkote Singhato, Kunchit Judprasong, Piyanut Sridonpai, Nunnapus Laitip, Nattikarn Ornthai, Charun Yafa
Summary: Fish are a significant source of protein for Thai people due to their availability and affordability. This study investigated the chemical forms of selenium in the ten most commonly consumed freshwater and marine fish in Thailand. The results showed that organic forms of selenium, such as selenomethionine and selenocystine, were the major forms present in most fish species.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Chemistry, Analytical
Herick Macedo Santos, Julymar Marcano Higuera, Ana Rita Araujo Nogueira
Summary: Multi-energy calibration (MEC) and high matrix introduction (HMI) system were used to directly analyze simulated gastrointestinal chyme and estimate the bioaccessibility of essential elements and As in shrimp. The results showed that the proposed procedures were accurate and efficient, with no significant differences compared to the reference method. Heat treatment increased the bioaccessibility of Cu and Fe, but decreased the bioaccessibility of Zn. The procedures provide simplicity and high sample throughput for elemental bioaccessibility studies.
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2023)
Article
Environmental Sciences
Martha Gledhill, Kechen Zhu, Dagmara Rusiecka, Eric P. Achterberg
Summary: In this study, we analyzed the concentrations and identities of siderophores in samples from the Northwest European shelf. We found 24 different siderophores and discovered that their impact on iron biogeochemistry is low at low concentrations, with iron bound to humic like dissolved organic matter being the dominant species.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Environmental Sciences
M. Ribeiro, N. Zephyr, J. A. L. Silva, M. Danion, T. Guerin, I Castanheira, A. Leufroy, P. Jitaru
Summary: The study found that MeHg bioaccumulates steadily in fish muscles when exposed individually, but the bioaccumulation is reduced when fish are first exposed to SeMet and then to MeHg, indicating a detoxifying effect of SeMet on MeHg.
Article
Spectroscopy
Davide Spanu, Stefano Fantinuoli, Gilberto Binda, Laura Rampazzi, Damiano Monticelli, Sandro Recchia
Summary: This study reports the development and validation of a sensitive, fast, high throughput and cost-effective method for the selective determination of Sb(III) and Sb(V) in water samples. The method separates the two species using a homemade cation exchange column and detects them using ICP-MS. The method offers very low detection limits, fast analysis time, and favorable instrumental configuration.
SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY
(2023)
Review
Chemistry, Multidisciplinary
Laura Favilli, Agnese Giacomino, Mery Malandrino, Paolo Inaudi, Aleandro Diana, Ornella Abollino
Summary: This review summarizes different approaches for the quantification of mercury species in different samples using HPLC-ICP-MS, and discusses the sampling and pretreatment steps prior to quantification.
FRONTIERS IN CHEMISTRY
(2022)
Article
Biochemical Research Methods
Sebastian Fassbender, Marcus von der Au, Maren Koenig, Jurgen Pelzer, Christian Piechotta, Jochen Vogl, Bjoern Meermann
Summary: The newly introduced ICP-ToF-MS instrument demonstrates enhanced sensitivity and superior isotope ratio precision, making it suitable for species-specific isotope dilution analysis, especially when analyzing multiple isotopic systems simultaneously.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Bernhard Michalke
Summary: Element analysis in clinical or biological samples plays a crucial role in clinical diagnostics, drug development, and drug-effect monitoring. By combining sophisticated sample delivery systems with ICP-MS, it is possible to gain deeper insights into the specific forms of element binding, the actual redox state, and the spatial distribution in tissue or single cells. This can provide a better understanding of biological processes and their dynamics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Vladimir N. Losev, Svetlana L. Didukh-Shadrina, Anastasia S. Orobyeva, Sergey I. Metelitsa, Elena V. Borodina, Urana V. Ondar, Pavel N. Nesterenko, Natalia V. Maznyak
Summary: A simple and efficient method for the determination of highly toxic arsenic species using noncovalently aminated silica was proposed. The method allows for the effective preconcentration of arsenic anionic species from aqueous solutions, with separate preconcentration and determination of As(V) and As(III) at pH 5.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Tom Van Helden, Simone Braeuer, Thibaut Van Acker, Olivier Leroux, Dominique Van Der Straeten, Frank Vanhaecke
Summary: This study investigates the effects of instrumental setup on the response duration of elements, such as Hg and Se, in biological tissue. By optimizing the setup, significant improvement in the response duration is achieved. However, caution should be taken when selecting instrument settings and data acquisition conditions for accurate elemental mapping to avoid smearing effects.
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2022)
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).