Article
Biophysics
Donato Calabria, Elisa Lazzarini, Andrea Pace, Ilaria Trozzi, Martina Zangheri, Stefano Cinti, Marinella Difonzo, Giovanni Valenti, Massimo Guardigli, Francesco Paolucci, Mara Mirasoli
Summary: A portable, 3D-printed miniaturized electrochemiluminescence (ECL) biosensor for glucose detection has been developed using carbon black-doped polylactic acid (PLA) electrodes. The ECL emission is obtained using a 1.5V AA alkaline battery and detected with a smartphone camera, enabling easy portability. The biosensor showed excellent ability to detect glucose and can be used for point-of-care analysis by preloading the necessary reagents.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Laura Sakalauskiene, Benediktas Brasiunas, Anton Popov, Asta Kausaite-Minkstimiene, Almira Ramanaviciene
Summary: The development of a first-generation reagentless amperometric glucose biosensor based on the combination of AuNS, PB, and GOx has been achieved, enabling convenient and intuitive glucose detection. The biosensor exhibits a wide linear range, good repeatability, and high selectivity.
Article
Biophysics
Wei Yang, Jianwen Fei, Wei Xu, Hongyan Jiang, Marwan Sakran, Junli Hong, Wanying Zhu, Xuemin Zhou
Summary: A biosensor based on Fe3O4@MnO2 NS was constructed for colorimetric determination of uric acid in plasma. The method allows quantitative detection of UA through specific catalytic reactions, and colorimetric analysis can be performed through naked eye, smartphone, and UV-vis spectrophotometer.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Chemistry, Analytical
Son Hai Nguyen, Phan Kim Thi Vu, Hung Manh Nguyen, Mai Thi Tran
Summary: In this study, a glucose sensor based on chitosan-capped ZnS-doped Mn nanomaterials was proposed. The sensor can quickly, simply, and reliably detect glucose concentrations in the range of 0.125 to 0.636 mM, corresponding to 2.3 to 11.4 mg/dL. The results showed that 1% chitosan-capped ZnS-doped Mn was the most sensitive, selective, and stable material.
Article
Biophysics
Elnaz Zeynaloo, Elsayed M. Zahran, Yu-Ping Yang, Emre Dikici, Trajen Head, Leonidas G. Bachas, Sylvia Daunert
Summary: This study introduces a new method of using unnatural amino acids as protein labels, which not only changes the local microenvironment but also has minimal impact on the overall structure. By incorporating L-DOPA into the protein, it can be used as an electrochemical label for highly selective and sensitive detection of glucose.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Physical
Gregory T. Carroll, Reed C. Dowling, David L. Kirschman, Mark B. Masthay, Angela Mammana
Summary: In this study, it was found that both natural and synthetic DNA exhibit a fluorescent photoproduct upon UV irradiation. Calf thymus DNA and poly(dA).poly(dT) showed significant fluorescence after being exposed to UV light, while poly(dAdT)2 displayed fluorescence to a lesser degree. Poly(dGdC)2 did not exhibit fluorescence, but the UV-Vis data indicated photochemical changes. The results indicate that fluorescence occurs when thymine and adenine base pairs are present, and is enhanced when thymine nucleobases are adjacent to each other on the same strand.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
(2023)
Article
Chemistry, Analytical
Leticia Tessaro, Adriano Aquino, Pedro Panzenhagen, Alan Clavelland Ochioni, Yhan S. Mutz, Paulo A. Raymundo-Pereira, Italo Rennan Sousa Vieira, Natasha Kilsy Rocha Belem, Carlos Adam Conte-Junior
Summary: This study investigated the use of functionalized AuNPs as plasmonic nanobiosensors to detect SARS-CoV-2. The nanobiosensor showed excellent sensitivity in detecting the virus in a dynamic concentration range and demonstrated 100% accuracy compared to the standard RT-qPCR technique. It provides a viable alternative for the rapid detection of SARS-CoV-2 on the surfaces of vegetables.
Article
Spectroscopy
Abdu Saeed, Fouad Abolaban
Summary: The study irradiated 20 rats with fast neutrons, finding that low-dose fast neutrons can cause tiny changes in the structure of hemoglobin, including alterations in sulfur-hydrogen bonds and the oxidation state of iron atoms. UV-Vis spectroscopy revealed that the irradiated rats might be more susceptive to oxidation.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Materials Science, Multidisciplinary
M. O. Kalinkin, D. A. Akulov, N. I. Medvedeva, R. M. Abashev, M. A. Melkozerova, E. V. Zabolotskaya, A. I. Surdo, D. G. Kellerman
Summary: This study examined the optical properties and defect structure of Li1-xNaxMgPO4 solid solutions using experimental and theoretical methods and found that the thermoluminescence of phosphate significantly increased with an increase in sodium concentration, which could be attributed to an increase in the number of oxygen vacancies. It was also discovered that sodium substitution could enhance the solubility of rare earth elements in LiMgPO4, leading to improved dosimetric characteristics.
MATERIALS TODAY COMMUNICATIONS
(2022)
Article
Chemistry, Physical
Luca Botti, Sebastian Meier, Ceri Hammond
Summary: This study investigates the catalytic conversion of glucose using Lewis acidic silicates in a continuous flow reactor with operando UV-vis spectroscopy. Transient absorption features related to glucose activation and conversion at various operational conditions are identified, and the deactivation of the stannosilicate catalyst is shown to affect the overall performance of the glucose upgrading reaction during continuous operation. The study highlights the nonuniform deactivation of the catalyst and its implications for improving the selectivity of catalysts for glucose upgrading.
Article
Engineering, Environmental
Xu-Dan Yang, Bo Gong, Wei Chen, Chen Qian, Meng Du, Han-Qing Yu
Summary: Microplastics serve as a source of organic contaminants in aquatic environments, impacting their fate and toxicity. To assess the environmental risks associated with microplastics, a method for evaluating the dynamic uptake of organic contaminants onto suspended microplastics in water is necessary. In this study, a UV-vis spectroscopy-based approach was developed to monitor the uptake of organic contaminants onto suspended microplastics in real-time.
Article
Chemistry, Analytical
Sumayya Binti Abd Azis, Nur Syafika, Hanin Azka Qonita, Tiara Resky Anugrah Mahmud, Ahmad Abizart, Andi Dian Permana
Summary: Glucose-responsive microparticles (GR-MP) containing metformin (MTF) were developed for oral drug delivery and to overcome the limitations of MTF. An analytical method was developed and validated to quantify MTF in GR-MP preparations, and to measure drug release and permeation in hyperglycemic conditions. The method showed accuracy and precision, and successfully supported the development of GR-MP containing MTF for controlled drug release.
MICROCHEMICAL JOURNAL
(2022)
Article
Engineering, Electrical & Electronic
Liang Wu, Yeqing Jin, Hanchao Guo, Jiale Wang, Feng Zhang, Qian Zhang, Mengdi Li
Summary: This article proposes an on-orbit calibration method for star sensors based on microvariation in intrinsic parameters (IPV method). By establishing a mathematical model and combining with the Kalman filter method, the accuracy of star sensor attitude can be improved.
IEEE SENSORS JOURNAL
(2023)
Article
Chemistry, Analytical
Kuan-Ying Lin, Yi-Hsing Tseng, Kai-Wei Chiang
Summary: The precision modelling of intrinsic camera geometry is a common issue in the fields of photogrammetry and computer vision. This paper clarifies the definitions of intrinsic camera parameters used in each field and proposes an algorithm to transform these parameters. The proposed transformation method is more rigorous than conventional methods and enables users to apply mixed software in the fields of photogrammetry and computer vision.
Article
Engineering, Electrical & Electronic
Imed Sassi, Mounir Ben El Hadj Rhouma
Summary: This study investigates glucose and hemoglobin detection in blood samples and other liquids using a novel SPR sensor. The sensor design incorporates layers of silver, titanium dioxide, black phosphorus, and gold. Simulation results demonstrate the impact of different materials on sensitivity performance. The proposed sensor achieves high sensitivity and shows potential for detecting biological or chemical entities in liquids.
OPTICAL AND QUANTUM ELECTRONICS
(2023)
Article
Biochemical Research Methods
Vicente L. Cebolla, Carmen Jarne, Jesus Vela, Rosa Garriga, Luis Membrado, Javier Galban
Summary: This review highlights the contribution of HPTLC in lipid analysis, especially its hyphenation with Mass Spectrometry and use of biological assays and isotopically labeled lipids. The described techniques allow for qualitative and quantitative information on lipids in complex matrices, with applications in molecular biology and biochemistry-related samples.
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
(2021)
Article
Chemistry, Inorganic & Nuclear
Lara Cancela, Miguel A. Esteruelas, Javier Galban, Montserrat Olivan, Enrique Onate, Andrea Velez, Juan C. Vidal
Summary: The influence of a metal center on mixed valence binuclear polyhydrides was explored by investigating the reactions of polyhydrides OsH6((PPr3)-Pr-i)(2) (1) and IrH5((PPr3)-Pr-i)(2) (2) with rollover cyclometalated hydride complexes. It was found that the metal center can promote the activation of specific bonds and lead to the formation of different numbers of hydrides in reactions with complexes 1 and 2.
INORGANIC CHEMISTRY
(2021)
Article
Biochemical Research Methods
Carmen Jarne, Luis Membrado, Maria Saviron, Jesus Vela, Jesus Orduna, Rosa Garriga, Javier Galban, Vicente L. Cebolla
Summary: In this study, 19 molecular species of globotriaosylceramides (Gb(3)) were identified in extracts from a Fabry's plasma patient and a healthy control using High-Performance Thin-Layer Chromatography (HPTLC)-mass spectrometry techniques. The analysis revealed different concentrations and distributions of these species in the plasma of Fabry's disease patients compared to healthy individuals.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Sofia Oliver, Susana de Marcos, Isabel Sanz-Vicente, Vicente Cebolla, Javier Galban
Summary: An enzymatic method for minimally invasive tyramine determination in cheese is proposed, where colorimetric test strips with tyramine oxidase and peroxidase are used for measurement. A spectrophotometric method has also been developed for tyramine determination in cheese extracts, with a mathematical model linking RGB signals to tyramine concentrations and spectroscopic properties. This method provides a quick and accurate way to detect tyramine levels in cheese samples.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Multidisciplinary
Alba Martin-Barreiro, Rogelio Soto, Stefano Chiodini, Aitor Garcia-Serrano, Santiago Martin, Lucia Herrer, Francesc Perez-Murano, Paul J. Low, Jose Luis Serrano, Susana Marcos, Javier Galban, Pilar Cea
Summary: Deposition of a top-contact electrode on a large-area monolayer electrode junction is a contemporary challenge for integrating molecular electronic phenomena into device structures. A two-step self-assembly process with uncapped gold nanoparticles results in promising electrical characteristics, paving a path for the fabrication of molecular electronic devices using simple methodologies.
ADVANCED MATERIALS INTERFACES
(2021)
Article
Biochemical Research Methods
Alba Martin-Barreiro, Susana de Marcos, Javier Galban
Summary: An enzymatic-colorimetric method based on the reaction between L-phenylalanine and L-amino acid oxidase in the presence of Au(III) has been developed, leading to the formation of gold nanoparticles. The intensity of the generated nanoparticles' localized surface plasmon resonance band can be related to the concentration of L-phenylalanine in the sample. The method allows for the determination of L-phenylalanine in a specific concentration range and is free from interference from other amino acids.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Pablo Cebrian, Leticia Perez-Sienes, Isabel Sanz-Vicente, Angel Lopez-Molinero, Susana de Marcos, Javier Galban
Summary: This article discusses the importance of digital image colorimetry (DIC) on smartphones as an alternative analytical technique. It proposes a methodology for standardization and correction of the problems associated with DIC, and demonstrates its effectiveness through three different colorimetric analyses using various smartphones. The study highlights the potential application of this measurement methodology in different sectors.
Article
Biophysics
Javier Camacho-Aguayo, Susana de Marcos, Veronica Mora-Sanz, Javier Galban
Summary: This paper explores the use of flavo-enzymes for enzymatic generation of gold nanomaterials for the first time. It demonstrates that in the presence of Au(III), gold nanomaterials can be formed in-situ during the oxidation of glucose by GOx. The obtained gold nanomaterials can be either nanoparticles (AuNPs) or nanoclusters (AuNCs), depending on the experimental conditions and the concentration of glucose. Mathematical equations describing the fluorescence and absorbance values during the reaction have been proposed. The method has also been successfully applied to determine glucose in orange juice and human plasma samples, showing no significant differences compared to other methods.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biochemical Research Methods
Javier Camacho-Aguayo, Susana de Marcos, Marta Pericas, Javier Galban
Summary: This study demonstrates the enzymatic synthesis of fluorescence nanomaterials and their application for substrate quantification. Specifically, fluorescent copper nanoclusters were synthesized during the enzymatic reaction of tyramine oxidase and tyramine in the presence of Cu(II), with the fluorescence intensity being directly proportional to the tyramine concentration. The formed nanoclusters were characterized and the formation mechanism was studied, revealing the reducing properties of the enzymatic reaction product that contributed to the formation. The developed method showed excellent sensitivity, precision, and has been successfully applied to the quantification of tyramine in sausage.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Isabel Sanz-Vicente, Irina Rivero, Lucia Marcuello, Maria Pilar Montano, Susana de Marcos, Javier Galban
Summary: Tyramine oxidase (TAO), peroxidase (HRP), and Amplex Red (AR) were immobilized on cellulose to create disposable biosensors for histamine determination. The enzymatic reaction produced a pink spot through the oxidation of AR. By using a smartphone to measure the G (green) color coordinate, histamine can be quantified in the presence of other biogenic amines in a range of concentrations, with a low limit of detection. The method was successfully applied to determine tyramine and histamine in a tuna extract, showing good results compared to the reference HPLC-MS method.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Mario Dominguez, Sofia Oliver, Rosa Garriga, Edgar Munoz, Vicente L. Cebolla, Susana de Marcos, Javier Galban
Summary: This study reports the development of simple, low-cost optical nanosensors for the semi-quantitative or naked-eye detection of tyramine in food spoilage. The sensors utilize Au(III)/tectomer films deposited on PLA supports, and the tyramine concentration can be determined by the color change of gold nanoparticles, which is measured by a smartphone color recognition app. The method also allows for more accurate quantification of tyramine using the reflectance of sensing layers and the absorbance of the characteristic plasmon band of gold nanoparticles at 550 nm.
Article
Chemistry, Applied
Irene Melendo, Javier Camacho-Aguayo, Sareh Paziresh, Sara Fuertes, Antonio Martin, Susana de Marcos, Javier Galban, Violeta Sicilia
Summary: New beta-diketonate platinum (II) complexes with bright phosphorescent emission and high quantum yield were synthesized and characterized. One of these complexes was used as a dye for sensing glucose levels.
Article
Chemistry, Analytical
Jesus Navarro, Gemma Cepria, Javier Camacho-Aguayo, Santiago Martin, Alejandro Gonzalez Orive, Susana de Marcos, Javier Galban
Summary: In this manuscript, a method for the fluorometric determination of tyramine is described. The method utilizes the direct reaction between Au(III) and tyramine to produce fluorescent gold nanoclusters, allowing for fast and stable detection of tyramine. The method shows high accuracy and sensitivity, making it suitable for the determination of tyramine in food samples.
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).
