Article
Biochemistry & Molecular Biology
Zheng Wei, Xiaoping Cai, Weifeng Cui, Junping Zhang
Summary: A CA19-9 electrochemical immunosensor was developed using a self-assembled membrane modified with a gold electrode. It demonstrated good specificity, strong anti-interference ability, and high precision and accuracy. The results showed its potential application value.
Article
Chemistry, Multidisciplinary
Rocco Cancelliere, Terenzio Cosio, Elena Campione, Martina Corvino, Maria Pia D'Amico, Laura Micheli, Emanuela Signori, Giorgio Contini
Summary: This study developed a low-cost, simple-to-manufacture, and user-friendly label-free electrochemical device for rapid detection of interleukin-6 (IL-6) in psoriasis patients. The immunosensor showed robust analytical performance with a wide linear range and good limit of detection and reproducibility. Compared to the traditional ELISA method, the label-free device has the advantages of ease of use and quicker detection time.
FRONTIERS IN CHEMISTRY
(2023)
Article
Chemistry, Physical
Yurong Zhao, Xuzhi Hu, Limin Zhang, Dong Wang, Stephen M. King, Sarah E. Rogers, Jiqian Wang, Jian R. Lu, Hai Xu
Summary: Controlling the dimension of nanostructures self-assembled from short peptides remains a challenge, with peptide bolaamphiphiles forming nanotubes due to the interplay between side chain structure and hydrophobicity; changes in amino acid side chain structure can significantly impact nanostructure formation, with the introduction of hydroxyl groups alleviating steric hindrance.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Nanoscience & Nanotechnology
Baolin Zhao, Bastian Gothe, Arthur Groh, Thomas Schmaltz, Johannes Will, Hans-Georg Steinrueck, Tobias Unruh, Stefan Mecking, Marcus Halik
Summary: This study demonstrates the use of phosphonic acid oligomers in self-assembled monolayer field-effect transistors, showing increased mobility in devices by controlling the thiophene unit counts. X-ray techniques reveal the molecular-scale packing and order of SAMs with different chain lengths.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Nanoscience & Nanotechnology
Julian M. Dlugosch, Henning Seim, Achyut Bora, Takuya Kamiyama, Itai Lieberman, Falk May, Florian Mueller-Plathe, Alexei Nefedov, Saurav Prasad, Sebastian Resch, Kai Saller, Christian Seim, Maximilian Speckbacher, Frank Voges, Marc Tornow, Peer Kirsch
Summary: This article presents a prototype of a ferroelectric tunnel junction (FTJ) based on a self-assembled monolayer (SAM) of small, functional molecules. The SAM is highly uniform and thin, and exhibits reversible conductance switching. Compared to inorganic material-based FTJs, this approach using SAMs of small organic molecules allows for a higher level of functional complexity while maintaining a robust and simple fabrication process. The technology is expected to find applications in information storage and processing.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Optics
Zhengkun Wang, Haiyang Sha, Ke Yang, Yong Zhu, Jie Zhang
Summary: In this study, the plasmonic response of self-assembled monolayer silver nanoparticles was comprehensively investigated using various computational and experimental techniques. The Fano resonance and SERS application were studied, and it was found that the higher-energy antibonding mode is not only from the antibonding dipole-dipole mode, but also from the antibonding quadrupole-quadrupole mode and hybridized magnetic mode. The effects of dimer gap and size were discussed in detail, and a two coupled oscillators model was used to illustrate the mechanism of the Fano resonance.
OPTICS AND LASER TECHNOLOGY
(2023)
Article
Chemistry, Physical
Jianfeng Li, Wei Cao, Jinjin Li, Ming Ma
Summary: The study revealed that fluorination of perfluorocarbon SAM enhances superlubricity performance, achieving significantly lower friction coefficient and greater load-bearing capacity between graphite in water.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Jie Xiao, Muyu Cong, Minmin Li, Xin Zhang, Yahui Zhang, Xinjia Zhao, Wenqi Lu, Zhimou Guo, Xinmiao Liang, Guangyan Qing
Summary: This study reports the development of a heterogeneous subnanochannel membrane formed by coating a functionalized self-assembled metal-organic framework (MOF) monolayer (SAMM) film on a porous anodic aluminum oxide membrane. The membrane exhibits excellent capacity for anion-selective transport and has the potential for significant advancement in osmotic power.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Shakil N. N. Afraj, Chun-Hsiao Kuan, Jian-Sing Lin, Jen-Shyang Ni, Arulmozhi Velusamy, Ming-Chou Chen, Eric Wei-Guang Diau
Summary: Four X-shaped quinoxaline-based organic dyes, PQx (1), TQx, (2), PQxD (3), and TQxD (4), are developed as p-type self-assemble monolayer (SAM) for tin perovskite solar cells (TPSC). The thermal, optical, and electrochemical properties of these SAMs are thoroughly investigated. Tin perovskite layers are successfully deposited on these SAM surfaces using a two-step approach, and the achieved device performance follows the order of TQxD (8.3%) > TQx (8.0%) > PQxD (7.1%) > PQx (6.1%). Among them, TQxD (4), with a thiophene pi-extended conjugation unit in the SAM structure, exhibits the highest hole extraction rates, greatest hole mobilities, and slowest charge recombination, leading to the best reported efficiency of 8.3% for SAM-based TPSC. Moreover, all devices except PQx show good enduring stability, retaining approximately 90% of their original values after 1600 hours of shelf storage.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Analytical
Tsung-Han Lee, Lung-Chieh Chen, Erick Wang, Chin-Cheng Wang, Yan-Ren Lin, Wen-Liang Chen
Summary: An electrochemical immunosensor was developed for rapid and accurate detection of cardiac troponin I in pre-hospital settings, showing highly correlated results with standard clinical laboratory methods. This suggests that the proposed point-of-care immunosensor can provide fast and accurate cTnI analysis for rapid diagnosis and patient management.
Article
Chemistry, Physical
Yiwen Wang, Wenlin Jiang, Shi-Chun Liu, Chieh-Ting Lin, Baobing Fan, Yanxun Li, Huanhuan Gao, Ming Liu, Francis R. Lin, Alex K. -Y. Jen
Summary: The operational stability of organic photovoltaics (OPVs) remains a major challenge for commercialization. By replacing the conventional hole-transporting layer with self-assembled monolayers of specific phosphonic acids, the lifetime and efficiency of OPV devices can be significantly improved. The improved performance is attributed to the stabilized vertical distribution of donor and acceptor components, reducing energetic disorder and non-radiative recombination losses. The application of these phosphonic acids in ternary devices demonstrates even higher efficiency and longer lifetime.
ADVANCED ENERGY MATERIALS
(2023)
Article
Medical Laboratory Technology
George J. Kahaly, Johannes Lotz, Sara Walder, Cara Hammad, Rebecca Kraemer, Lara Frommer, Jochem Koenig, Jan Wolf, Ulrike Gottwald-Hostalek, Bogumila Urgatz, Karl J. Lackner
Summary: This study evaluated the analytical performance of a new point-of-care (POC) thyrotropin (TSH) assay called Wondfo. The results showed that the Wondfo system was user-friendly and demonstrated analytical performance suitable for the prompt diagnosis of thyroid dysfunction.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2022)
Article
Chemistry, Physical
Zijing Chen, Yiming Li, Zhenghao Liu, Jiangjian Shi, Bingcheng Yu, Shan Tan, Yuqi Cui, Chengyu Tan, Fubo Tian, Huijue Wu, Yanhong Luo, Dongmei Li, Qingbo Meng
Summary: In this study, a functionalized C-PCBA SAM is introduced to improve interface defects and electron transportation in perovskite solar cells, resulting in high efficiency and stability.
ADVANCED ENERGY MATERIALS
(2023)
Article
Chemistry, Physical
L. E. Lopez-Gonzalez, J. Guerrero-Sanchez, H. Tiznado
Summary: In this study, Stearic acid (SA) self-assembled monolayer (SAM) was proposed as a soft-removal passivating agent for area selective atomic layer deposition (AS-ALD) applications. The experimental results showed that SA SAM formed a highly packed, hydrophobic, and ordered monolayer on a copper surface, transitioning to disorder above 160 degrees C. Furthermore, SA demonstrated good stability as a non-growth surface for ZnO ALD, and could be completely removed with ethanol at room temperature.
SURFACES AND INTERFACES
(2023)
Article
Chemistry, Analytical
Emiliano Felici, Matias D. Regiart, Sirley V. Pereira, Francisco G. Ortega, Lucio Angnes, German A. Messina, Martin A. Fernandez-Baldo
Summary: In this study, a portable and reusable microfluidic device for prostate-specific antigens (PSA) quantification was developed. The device combined a polymethyl methacrylate microfluidic platform with electrochemical detection. By modifying the platinum working microelectrode with carbon nanofibers (CNF)-decorated gold nanoporous (GNP) structures, the device exhibited enhanced properties such as increased antibody immobilization capacity and electroactive area. The device showed a detection limit of 5 pg mL(-1) and a linear range from 0.01 to 50 ng mL(-1). Clinical testing in serum from 30 prostate cancer patients demonstrated the device's sensitivity, selectivity, and portability for diagnosis and monitoring.
Review
Chemistry, Analytical
Elba Mauriz, Laura M. Lechuga
Summary: This review summarizes the latest advances in plasmonic technologies for monitoring clinically relevant biomarkers at the single-molecule level, discussing design and functional applications targeting individual molecules, and a wide variety of analytes involved in new analytical developments. It also provides an overview of the utility of plasmonic-based single-molecule analysis for personalized medicine.
Article
Engineering, Electrical & Electronic
Bernat Bassols-Cornudella, Patricia Ramirez-Priego, Maria Soler, M-Carmen Estevez, Heriberto J. Diaz Luis-Ravelo, Maria Cardenosa-Rubio, Laura M. Lechuga
Summary: Biosensors employing photonics integrated circuits, specifically interferometric evanescent wave working principles, show excellent performance with high sensitivity and simple design. A new all-optical modulation and trigonometry-based algorithm has been successfully applied to the Bimodal Waveguide (BiMW) biosensor, enabling selective identification and quantification of the Spike (S) protein of SARS-CoV-2. The results demonstrate high sensitivity and specificity, paving the way for a point-of-care device for general use.
JOURNAL OF LIGHTWAVE TECHNOLOGY
(2022)
Article
Cell Biology
Manuel Olazabal-Moran, Miriam Sanchez-Ortega, Laura Martinez-Munoz, Carmen Hernandez, Manuel S. Rodriguez, Mario Mellado, Ana C. Carrera
Summary: PI3-kinase and PTEN regulate the PIP3/AKT signaling pathway through the ubiquitin ligase cCBL, influencing cell survival, division, and migration. These findings reveal a novel mechanism for PI3-kinase/PTEN crosstalk and suggest that cCBL may be a potential target for modulating PTEN activity in cancer.
Article
Chemistry, Analytical
Elba Mauriz, Laura M. Lechuga
Summary: This review highlights recent advancements in using Surface Plasmon Resonance biosensors for drug discovery of SARS-CoV-2 inhibitors, discussing various SPR assay formats based on binding kinetics and drug efficacies, with a special focus on targeting key proteins of the virus. It also reviews the functionality of plasmonic biosensors for high-throughput screening of entry virus inhibitors, considering experimental parameters, potential limitations, and future applications.
Article
Cell Biology
Alvaro Fernando Garcia-Jimenez, Yaiza Caceres-Martell, Daniel Fernandez-Soto, Pedro Martinez Fleta, Jose M. Casasnovas, Francisco Sanchez-Madrid, Jose Miguel Rodriguez Frade, Mar Vales-Gomez, Hugh T. Reyburn
Summary: Several unanswered questions remain about humoral and cellular immunity to SARS-CoV-2, including whether preexisting memory T or B cells in unexposed individuals can recognize and suppress COVID-19. Research has shown that while some unexposed individuals have cross-reactive T cell responses, these responses are weak in aspects like T-FH expansion, which may impact the generation of effective antibodies against SARS-CoV-2. Understanding these differences in cellular responses is crucial for advancing our knowledge of immunity to SARS-CoV-2.
JOURNAL OF LEUKOCYTE BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Elena R. Bovolenta, Eva M. Garcia-Cuesta, Lydia Horndler, Julia Ponomarenko, Wolfgang W. Schamel, Mario Mellado, Mario Castro, David Abia, Hisse M. van Santen
Summary: Signaling through T cell receptor (TCR) plays a critical role in the development, maintenance, and activation of T cells. This study reveals that the strength of pre-TCR-mediated signaling during T cell development determines the diversity of TCR beta repertoire for positive and negative selection.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Eva M. Garcia-Cuesta, Jose Miguel Rodriguez-Frade, Sofia R. Gardeta, Gianluca D'Agostino, Pablo Martinez, Blanca Soler Palacios, Graciela Cascio, Tobias Wolf, Nicolas Mateos, Rosa Ayala-Bueno, Cesar A. Santiago, Pilar Lucas, Lucia Llorente, Luis M. Allende, Luis Ignacio Gonzalez-Granado, Noa Martin-Cofreces, Pedro Roda-Navarro, Federica Sallusto, Francisco Sanchez-Madrid, Maria F. Garcia-Parajo, Laura Martinez-Munoz, Mario Mellado
Summary: This study reveals that the truncated mutant chemokine receptor CXCR4(R334X) associated with WHIM syndrome fails to nanoclusterize after CXCL12 stimulation, affecting cell spatial organization and mobility, and causing multiple phalloidin-positive protrusions in cells. The inappropriate activation of beta-arrestin1 by CXCR4(R334X) leads to inadequate actin cytoskeleton remodeling, disrupting the balance between activated and deactivated cofilin.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Nanoscience & Nanotechnology
Daheui Choi, Kihak Gwon, Hye Jin Hong, Harihara Baskaran, Olalla Calvo-Lozano, Alan M. Gonzalez-Suarez, Kyungtae Park, Jose M. de Hoyos-Vega, Laura M. Lechuga, Jinkee Hong, Gulnaz Stybayeva, Alexander Revzin
Summary: Human pluripotent stem cells have great potential for cell therapeutics and disease modeling. Researchers have developed heparin-containing microcapsules that promote stem cell aggregation and can deliver growth factors. They also found that coating the microcapsules with tannic acid can enhance their biological activity and increase pluripotency expression.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Immunology
Sofia R. Gardeta, Eva M. Garcia-Cuesta, Gianluca D'Agostino, Blanca Soler Palacios, Adriana Quijada-Freire, Pilar Lucas, Jorge Bernardino de la Serna, Carolina Gonzalez-Riano, Coral Barbas, Jose Miguel Rodriguez-Frade, Mario Mellado
Summary: The local lipid environment plays an important role in regulating CXCR4 organization and function, and modulating chemokine-triggered directed cell migration. Treatment of T cells with bacterial sphingomyelinase alters the lipid composition of the cell membrane, affecting membrane fluidity and CXCR4 nanoclustering and dynamics.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Analytical
Olalla Calvo-Lozano, Pablo Garcia-Aparicio, Lajos-Zsolt Raduly, Maria Carmen Estevez, Ioana Berindan-Neagoe, Manuela Ferracin, Laura M. Lechuga
Summary: Fast and accurate diagnosis of cancer is crucial for timely treatment and improved patient survival. This study presents a nanophotonic biosensor that can directly and in real-time detect a biomarker associated with lung cancer in human plasma. The biosensor achieves highly sensitive detection without the need for complex pre-processing steps.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Seonhwa Lee, Alan M. Gonzalez-Suarez, XuHai Huang, Olalla Calvo-Lozano, Sonja Suvakov, Laura M. Lechuga, Vesna D. Garovic, Gulnaz Stybayeva, Alexander Revzin
Summary: Extracellular vesicles (EVs) are lipid bilayer nanovesicles that contain important biological information for disease diagnosis. This paper presents a nanoparticle-enabled immunoassay integrated with an electrochemical plate for quantifying the expression of podocin and nephrin in urinary EVs. The electrochemical immunoassay revealed a higher podocin to nephrin ratio in preeclamptic women compared to healthy controls, suggesting the potential of this ratio for disease diagnosis.
Article
Nanoscience & Nanotechnology
Olalla Calvo-Lozano, Laura Hernandez-Lopez, Leyre Gomez, Arnau Carne-Sanchez, Cornelia von Baeckmann, Laura M. Lechuga, Daniel Maspoch
Summary: In this study, metal-organic cages or polyhedra (MOCs/MOPs) were integrated into a nanophotonic sensor for the rapid and real-time detection of small pollutant molecules in water. The sensor, which incorporates Rh(II)-based MOPs as chemical receptors, does not require sample pretreatment and provides fast and accurate results. The detection limits for two common pollutants, 1,2,3-benzotriazole (BTA) and imidacloprid (IMD), were both below the corresponding toxicity thresholds defined by the European Chemicals Agency (ECHA). This research opens the possibility of implementing competitive sensor devices for in situ environmental monitoring by combining innovative chemical receptors with state-of-the-art photonic sensing technologies.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Immunology
Blanca Soler Palacios, Ricardo Villares, Pilar Lucas, Jose Miguel Rodriguez-Frade, Ana Cayuela, Jonathan G. Piccirillo, Manuel Lombardia, David Delgado Gestoso, Miguel Fernandez-Garcia, Cristina Risco, Coral Barbas, Fernando Corrales, Carlos Oscar S. Sorzano, Nuria Martinez-Martin, Jose Javier Conesa, Francisco J. Iborra, Mario Mellado
Summary: This study found that growth hormone can alter the metabolism of inflammatory macrophages, increase mitochondrial volume and alter mitochondrial dynamics. It reduces glycolysis and lactate production, and increases the density of the inner mitochondrial membrane. The results suggest that targeting macrophage metabolism could be a new approach for intervening in inflammatory diseases.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Analytical
Olalla Calvo-Lozano, Pablo Garcia-Aparicio, Lajos-Zsolt Raduly, Maria Carmen Estevez, Ioana Berindan-Neagoe, Manuela Ferracin, Laura M. Lechuga
Summary: This study introduces a nanophotonic biosensor for the direct and real-time detection of microRNA biomarkers related to lung cancer in human plasma. Its application in clinical samples showed competitive detectability and significance compared to traditional molecular analysis methods like qRT-PCR.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).
