期刊
TALANTA
卷 77, 期 2, 页码 809-814出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2008.07.038
关键词
Chemiluminescence immunosensor; Liposome; Prostate-specific antigen; Enhanced chemiluminescence reaction
资金
- 973 National Key Basic Research Program [2007CB310500]
- National Nature Science Foundation of China [20775023, 20675028, 20435010]
A highly sensitive chemiluminescence immunosensor for the detection of prostate-specific antigen (PSA) was developed based on a novel amplification procedure with the application of enzyme encapsulated liposome. Horseradish peroxidase (HRP) encapsulated and antibody-modified liposome acts as the carrier of a large number of markers and specific recognition label for the amplified detection of PSA. In the detection of PSA, the analyte was first bound to the specific capture antibody immobilized on the microwell plates, and then sandwiched by the antibody-modified liposomes encapsulating HRP. The encapsulated markets, HRP molecules were released by the lysis of the specifically bound liposomes in the microwell with Triton X-100 solution. Then, the analyte PSA could be determined via the chemiluminescence signal of HRP-catalyzed luminol/peroxide/enhancer system. The sandwich-type immunoassay provides the amplification route for the PSA detection in ultratrace levels, The CL emission intensity exhibits dynamic correlation to PSA concentration in the range from 0.74 pg/ml to 0.74 mu g/ml with readily achievable detection limit of 0.7 pg/ml. (C) 2008 Elsevier B.V. All rights reserved.
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