期刊
TALANTA
卷 75, 期 5, 页码 1348-1355出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2008.01.055
关键词
dual enzymes based biosensor; direct electrochemistry; signal bioamplification; Sonogel-Carbon electrode
Direct electrochemistry of Trametes versicolor Laccase (LAC) was found at a Sonogel-Carbon electrode. The bioamplification, performed by dual immobilization of this enzyme and Mushroom Tyrosinase (TYR), of the bio-electrocatalytic reduction Of 02 was investigated. The calculated a transfer coefficients were 0.64 and 0.67, and the heterogeneous electron-transfer rate constants were 6.19 and 8.52 s(-1), respectively, for the individual LAC and dual LAC-TYR-based Nafion/Sonogel-Carbon bio-electrodes. The responses of the dual enzymes electrode to polyphenols were stronger than those of the individual LAC or TYR biosensors. Hypotheses are offered about the mechanism of bioamplification. The surfaces of the biosensors were also characterized by AFM. (c) 2008 Elsevier B.V. All rights reserved.
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