Article
Entomology
Liliana Toth, Peter Poor, Attila ordog, Gyorgyi Varadi, Attila Farkas, Csaba Papp, Gabor Bende, Gabor K. Toth, Gabor Rakhely, Florentine Marx, Laszlo Galgoczy
Summary: Plant pathogenic fungi cause significant losses in crop production worldwide. Developing new strategies for plant and crop protection is crucial due to the resistance of these fungi to approved chemical fungicides. A study investigated the potential of using extracellular antifungal proteins (AFPs) and their peptide derivatives (PDs) as combinatorial biofungicides for plant and crop protection. The study found that the combination of AFPs and PDs can effectively inhibit the growth of various fungi and mitigate the symptoms of fungal infection on tomato plants.
Article
Agronomy
Rita Buechner, Monika Voros, Henrietta Allaga, Andras Varga, Attila Bartal, Andras Szekeres, Sarolta Varga, Judit Bajzat, Nora Bakos-Barczi, Andras Misz, Csaba Csutoras, Lorant Hatvani, Csaba Vagvolgyi, Laszlo Kredics
Summary: This study isolated and examined bacterial strains from mushroom casing material for their potential use as biocontrol agents against mold pathogens of white button mushroom. The selected Bacillus velezensis strain showed effective control against the pathogen, and even increased the crop yield of A. bisporus. These findings provide a potential method for the biological and integrated treatment in Agaricus cultivation.
Article
Microbiology
Thu Huynh, Monika Voros, Orsolya Kedves, Adiyadolgor Turbat, Gyoergy Sipos, Balazs Leitgeb, Laszlo Kredics, Csaba Vagvolgyi, Andras Szekeres
Summary: This study developed an easy and fast-automated identification tool based on cellular fatty acid profiles to distinguish Bacillus velezensis and Bacillus amyloliquefaciens. The differences and similarities between the two species were identified through the analysis of fatty acid components.
Article
Pharmacology & Pharmacy
Sandor Vaczi, Lilla Barna, Krisztian Laczi, Ferenc Tomosi, Gabor Rakhely, Botond Penke, Livia Fulop, Ferenc Bogar, Tamas Janaky, Maria A. Deli, Zsofia Mezei
Summary: This study examined the effects of sigma-1 receptor ligands on platelet and vascular eicosanoid synthesis. The results showed that these ligands can modify the synthesis of eicosanoids in platelets and vessels and play a role in multiple steps of arachidonic acid metabolism.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Microbiology
Monika Homa, Sandugash Ibragimova, Csilla Szebenyi, Gabor Nagy, Nora Zsindely, Laszlo Bodai, Csaba Vagvolgyi, Gabor Nagy, Tamas Papp
Summary: This study reveals significant changes in gene expression of Mucor lusitanicus under aerobic and anaerobic conditions through transcriptomic analysis. Carbohydrate metabolism and transporter genes, especially sugar transporters, are found to be affected. Moreover, transcripts related to amino acid transport and metabolism, lipid transport and metabolism, and energy production and conversion are downregulated under anaerobic conditions.
Article
Biotechnology & Applied Microbiology
Nikolett Miklovics, Agnes Duzs, Fanni Balogh, Gabor Paragi, Gabor Rakhely, Andras Toth
Summary: This study reports the characteristics of the quinone binding site (QBS) of a type VI sulfide:quinone oxidoreductases (SQR) from the photosynthetic purple sulfur bacterium Thiocapsa roseopersicina. The study identifies the amino acids near the enzyme-bound quinone ring and their interaction with the quinone head group. Mutant experiments further confirm the importance of these interactions.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Etelka Kovacs, Csilla Szucs, Attila Farkas, Mark Szuhaj, Gergely Maroti, Zoltan Bagi, Gabor Rakhely, Kornel L. Kovacs
Summary: This study investigated the decomposition of lignocellulosic plant biomass by four filamentous fungi. It was found that the Penicillium aurantiogriseum strain exhibited excellent hydrolytic enzyme production and generated the highest biogas yield from partially degraded substrates. Corn stover was the most suitable substrate for biomass decomposition and biogas production.
JOURNAL OF BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Alajos Berczi, Zsuzsanna Marton, Krisztina Laskay, Andras Toth, Gabor Rakhely, Agnes Duzs, Krisztina Sebok-Nagy, Tibor Pali, Laszlo Zimanyi
Summary: Cytochrome b561 proteins are integral membrane proteins that have ascorbate reducibility and trans-membrane electron transferring capability. They have been found in various animal and plant phyla and are localized in different membranes. Two homologous proteins in humans and rodents are thought to be involved in cancer pathology. This paper presents the physical-chemical properties of the homologues in mice and humans, providing insights into the CYB561 protein family.
Article
Biochemistry & Molecular Biology
Gyorgyi Varadi, Zoltan Kele, Andras Czajlik, Attila Borics, Gabor Bende, Csaba Papp, Gabor Rakhely, Gabor K. Toth, Gyula Batta, Laszlo Galgoczy
Summary: Due to the fast spread of drug resistance, only a limited number of drugs can be used to treat fungal infections. Therefore, there is an urgent need to develop new antifungal treatment strategies. The NFAP2 protein secreted by the mold Neosartorya shows promise as a template for future antifungal drug design, but it is difficult to determine its disulfide linkage pattern using traditional mass spectrometry-based methods.
Article
Food Science & Technology
Gabor Endre, Babett Edit Nagy, Daniel Hercegfalvi, Csenge Kasuba, Csaba Vagvolgyi, Andras Szekeres
Summary: Aflatoxins (AFs) are secondary metabolites that cause diseases in animals and humans. Regulations set concentration limits for AFs in EU food and feed products, requiring pure forms for reference standards. Improvements were made to a liquid-liquid chromatographic method to enhance purification and increase the yield of pure AFs. Through a scale-up process, approximately 2.2 g of AFs could be purified using 8.2 L of solvent in a 250 mL rotor, while 7.8 g of AFs could be prepared using 31 L of solvent in a 1000 mL rotor.
Article
Food Science & Technology
David Rakk, Jozsef Kukolya, Biljana D. Skrbic, Csaba Vagvolgyi, Monika Varga, Andras Szekeres
Summary: In this study, a 1-minute multi-mycotoxin analysis method was developed using high-resolution equipment and a short column. This method was able to detect 11 mycotoxins in corn and wheat matrices within one minute, utilizing the multiplexing and high-resolution capability of the QExactive Orbitrap MS.
Article
Pharmacology & Pharmacy
Balint Racz, Annamaria Kincses, Krisztian Laczi, Gabor Rakhely, Enrique Dominguez-alvarez, Gabriella Spengler
Summary: Recently, the anticancer activity of symmetrical selenoesters was investigated in sensitive and resistant human colon adenocarcinoma cells. The compounds showed synergistic interaction with doxorubicin and exhibited ABCB1 inhibitory and apoptosis-inducing effects. Transcriptome analysis revealed that symmetrical selenoesters may influence the redox state of cells and interfere with metastasis formation.
Article
Microbiology
Viktor David Nagy, Anuar Zhumakayev, Monika Voros, Adam Borde, Adrienn Szarvas, Attila Szucs, Sandor Kocsube, Peter Jakab, Tamas Monostori, Biljana D. Skrbic, Edina Mohai, Lorant Hatvani, Csaba Vagvolgyi, Laszlo Kredics
Summary: The cultivation and consumption of sweet potato are increasing globally, but the use of chemical fertilizers and pesticides may cause pollution. Therefore, there is a need for environmentally-friendly biological solutions. This study developed a microbial inoculant from multiple microorganisms and tested its application in sweet potato cultivation, resulting in increased yield. This is the first successful application of a fungal-bacterial consortium in sweet potato cultivation.
Article
Biotechnology & Applied Microbiology
Mark Szuhaj, Balazs Kakuk, Roland Wirth, Gabor Rakhely, Kornel Lajos Kovacs, Zoltan Bagi
Summary: This study investigated the biomethane formation from 4 H-2 + CO2 by two methanogens, Methanocaldococcus fervens and Methanobacterium thermophilum. The research aimed to understand the regulation of biomethane biosynthesis by H-2. It was found that the autotrophic methanogen M. fervens could be easily controlled by H-2/CO2, while the heterotrophic methanogen M. thermophilum was less sensitive to H-2/CO2, suggesting the importance of the biomethane-generating methanogenic community in renewable excess electricity conversion and storage systems.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Dora Balazs, Tamas Marik, Andras Szekeres, Csaba Vagvolgyi, Laszlo Kredics, Chetna Tyagi
Summary: This study investigated the production of peptaibols by various Trichoderma strains and their correlation with structure-activity relationships. The results showed that T. longibrachiatum f. bissettii SZMC 12546 exerted the lowest minimum inhibitory concentrations (MIC) against Gram-positive bacteria and also inhibited the plant pathogenic Gram-negative Rhizobium radiobacter. Molecular dynamics simulations revealed that the folding dynamics of Group A peptaibols were more restricted and had well-folded helical conformations, which may contribute to their bioactivity against bacteria.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)