Article
Microbiology
Gyu Ri Kim, Si Hyun Kim, Eun-Young Kim, Eun Hee Park, In Yeong Hwang, Seok Hoon Jeong, Hyun Soo Kim, Young Ah Kim, Young Uh, Kyeong Seob Shin, Young Ree Kim, Namhee Ryoo, Jong Hee Shin, Jeong Hwan Shin
Summary: This study evaluated the usefulness of MALDI-TOF VITEK MS for Salmonella identification. The results showed that VITEK MS could accurately identify most Salmonella species, but had lower accuracy in identifying typhoidal Salmonella and Salmonella enterica ssp. diarizonae isolates.
Article
Chemistry, Applied
Qiuxia Yang, Yushan Guo, Yueming Jiang, Bao Yang
Summary: Oligosaccharides are recognized as prebiotics and their structure is closely related to bioactivity. In this study, UPLC-MS/MS was developed to identify the structure of oligosaccharides, which mainly appeared as sodium adduct in the chromatogram. The fragmentation patterns of oligosaccharides were summarized for different glycosidic linkages. The results showed that UPLC-MS/MS was effective in identifying the structure of oligosaccharides and could be applied in polysaccharide structure identification.
FOOD HYDROCOLLOIDS
(2023)
Review
Environmental Sciences
Mohammad Y. Ashfaq, Dana A. Da'na, Mohammad A. Al-Ghouti
Summary: Bacteria play diverse roles in the environment, with MALDI-TOF MS technology gaining attention for routine bacterial identification. This technique has been applied in various environmental studies, showing benefits in rapid and cost-effective bacteria identification.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2022)
Article
Biochemistry & Molecular Biology
Xia Wang, Guanqi Wu, Shuo Han, Jingjing Yang, Xiangwei He, Haifang Li
Summary: Plant endophytic bacteria play important roles in plant growth and stress resistance. This study identified and classified endophytic bacteria in Populus based on MALDI-TOF MS data and 16S rRNA gene sequencing, and verified the classification capacity of protein mass fingerprints. The results provide a valuable reference for differentiating and identifying endophytic bacteria.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Marcelo de M. A. Coura, Eder A. Barbosa, Guilherme D. Brand, Carlos Bloch, Joao B. de Sousa
Summary: The incidence of colorectal cancer (CRC) is increasing in Brazil and there is a lack of reliable biomarkers for diagnosis and prognosis. Alterations in N-glycosylation profiles in serum and tissues of colon cancer patients were identified using mass spectrometry technologies. While serum N-glycans were mostly decreased, certain forms were overexpressed in tumor tissues. There was little correlation between tissue and serum N-glycosylation profiles in cancer patients. Total serum N-glycome analysis showed potential in discriminating patients from healthy controls, but compositions in serum did not parallel those found in tumor tissues.
Article
Chemistry, Organic
Bernard Fernandez, Jean Armengaud, Gilles Subra, Christine Enjalbal
Summary: This study investigates the feasibility of using high energy charge remote fragmentations to analyze the N-terminus of proteins, and compares it with low energy collision-induced dissociations. The results show that the amino acid composition significantly affects the dissociation pathways of peptide backbones.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2022)
Review
Chemistry, Analytical
Hugo M. Santos, Luis B. Carvalho, Carlos Lodeiro, Goncalo Martins, Ines L. Gomes, Wilson D. T. Antunes, Vanessa Correia, Maria M. Almeida-Santos, Helena Rebelo-de-Andrade, Antonio P. A. Matos, J. L. Capelo
Summary: This work presents a tutorial on the capabilities of bioanalytical mass spectrometry in detecting and differentiating viruses at the peptide level, as well as providing diagnosis and prognosis for infected patients. It offers insights into the possibilities of current high-resolution mass spectrometry technology and bioinformatics.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Jiaojiao Sun, Haimei Shi, Yuyan Xue, Wenmin Cheng, Menghuan Yu, Chuanfan Ding, Fuxing Xu, Shaoning Yu
Summary: The accuracy of bacterial identification based on MALDI-TOF MS can be affected by functional proteins, releasing bacteria from functional material can increase accuracy.
Review
Chemistry, Analytical
Judy Gopal, Manikandan Muthu
Summary: Mushrooms have transitioned from a luxury food to a widely accepted nutrient-rich food. Mushroom polysaccharides have become icons of mushroom bioactivity, and the analytical techniques for their analysis have been consolidated and summarized.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Microbiology
Alexandre Godmer, Yahia Benzerara, Anne Cecile Normand, Nicolas Veziris, Salah Gallah, Catherine Eckert, Philipe Morand, Renaud Piarroux, Alexandra Aubry
Summary: MALDI-TOF MS is commonly used in clinical microbiology labs for pathogen identification, but faces limitations, especially in uncertain identification of ECC species like E. hormaechei. By utilizing an online database combined with high-performance MSI software, substantial improvements in species-level identification of ECC isolates can be achieved.
MICROBIOLOGY SPECTRUM
(2021)
Article
Microbiology
Adriana Calderaro, Mirko Buttrini, Benedetta Farina, Sara Montecchini, Monica Martinelli, Federica Crocamo, Maria Cristina Arcangeletti, Carlo Chezzi, Flora De Conto
Summary: Colistin resistance is a major threat to global public health, and this study evaluated a MALDI-TOF mass spectrometry peak-based assay to distinguish colistin resistant and susceptible E. coli strains. A classifying algorithm model was developed, with SNN and GA algorithms showing the best performances in recognizing strains.
Article
Biochemistry & Molecular Biology
Pushpanjali Dasauni, Nirpendra Singh, Varun Chhabra, Manoranjan Mahapatra, Renu Saxena, Suman Kundu
Summary: A method using TFE and heat denaturation prior to digestion was developed and optimized for improved sequence coverage and analysis of Hb variants by nano LC-MALDI MS/MS. The TFE method provided the most suitable efficiency for Hb variant identification based on high sequence coverage.
Article
Microbiology
Arnaud Jabet, Anne-Cecile Normand, Alicia Moreno-Sabater, Jacques Guillot, Veronica Risco-Castillo, Sophie Brun, Magalie Demar, Romain Blaizot, Cecile Nabet, Ann Packeu, Renaud Piarroux
Summary: The objective of the study was to reduce recurrent errors between phylogenetically close species by adding new reference spectra. Diversifying the references and adding spectra from various culture media and culture durations improved identification performance, although confusion remained between certain closely related species.
Article
Microbiology
Camila Morschbacher Wilhelm, Maiara dos Santos Carneiro, Everton Inamine, Afonso Luis Barth
Summary: This study aimed to evaluate an adaptation of the MBT-ASTRA technique for faster antimicrobial susceptibility testing. The adapted method showed high categorical agreement with the standard method, indicating its potential for rapid AST results.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Victoria Girard, Valerie Monnin, Delphine Giraud, Sophie Polsinelli, Marion Caille, Gary W. Procop, Marion Tuohy, Deborah Wilson, Sandra S. Richter, Katalin Kiss, Kimberly Clem, Nicholas Tolli, Laurence Bridon, Constance Bradford, Sara Blamey, Jay Li, David H. Pincus
Summary: The use of MALDI-TOF for clinical isolates has been proven to be rapid and accurate, with the VITEK MS v3.2 database showing high accuracy in identifying a wide range of isolates. The study revealed that the majority of isolates were identified to species or group level, with a small percentage of isolates identified only to the genus level. The ability of VITEK MS to identify various bacteria and fungi, including emerging and highly pathogenic species, highlights its potential as a valuable tool in clinical laboratories.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2021)
Article
Infectious Diseases
C. Mirande, I. Canard, S. Buffet Croix Blanche, J. -P. Charrier, A. van Belkum, M. Welker, S. Chatellier
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2015)
Article
Microbiology
Jennifer Mesureur, Sebastien Ranaldi, Valerie Monnin, Victoria Girard, Sandrine Arend, Martin Welker, David O'Callaghan, Jean-Philippe Lavigne, Anne Keriel
JOURNAL OF CLINICAL MICROBIOLOGY
(2016)
Article
Microbiology
Shuping Nie, Baoyu Tian, Xiaowei Wang, David H. Pincus, Martin Welker, Kathleen Gilhuley, Xuedong Lu, Yiping W. Han, Yi-Wei Tang
JOURNAL OF CLINICAL MICROBIOLOGY
(2015)
Letter
Microbiology
Alex van Belkum, Martin Welker, W. Michael Dunne, Victoria Girard
JOURNAL OF CLINICAL MICROBIOLOGY
(2015)
Article
Infectious Diseases
Victoria Girard, Sandrine Mailler, Martin Welker, Maud Arsac, Beatrice Celliere, Pierre-Jean Cotte-Pattat, Sonia Chatellier, Geraldine Durand, Anne-Marie Beni, Jacques Schrenzel, Elizabeth Miller, Rahima Dussoulier, W. Michael Dunne, Susan Butler-Wu, Michael A. Saubolle, Den Sussland, Melissa Bell, Alex van Belkum, Parampal Deol
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2016)
Article
Infectious Diseases
J. Veenemans, M. Welker, A. van Belkum, M. C. Saccomani, V. Girard, A. Pettersson, C. Verhulst, M. Kluytmans-Vandenbergh, J. Kluytmans
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2016)
Article
Infectious Diseases
Asa Lindgren, Nahid Karami, Roger Karlsson, Christina Ahren, Martin Welker, Edward R. B. Moore, Liselott Svensson Stadler
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2018)
Article
Environmental Sciences
Danka Harustiakova, Martin Welker
ENVIRONMENTAL MICROBIOLOGY REPORTS
(2017)
Review
Medical Laboratory Technology
Alex van Belkum, Martin Welker, David Pincus, Jean-Philippe Charrier, Victoria Girard
ANNALS OF LABORATORY MEDICINE
(2017)
Article
Microbiology
Ruxin Li, Di Xiao, Jing Yang, Suju Sun, Samuel Kaplan, Zhirong Li, Yanan Niu, Cuixin Qiang, Yu Zhai, Xiaoming Wang, Xingzhen Zhao, Baoxin Zhao, Martin Welker, David H. Pincus, Dazhi Jin, Mini Kamboj, Guanghui Zheng, Guojun Zhang, Jianzhong Zhang, Yi-Wei Tang, Jianhong Zhao
JOURNAL OF CLINICAL MICROBIOLOGY
(2018)
Review
Biochemical Research Methods
Martin Welker, Alex Van Belkum, Victoria Girard, Jean-Philippe Charrier, David Pincus
EXPERT REVIEW OF PROTEOMICS
(2019)
Review
Microbiology
Martin Welker, Alex van Belkum
FRONTIERS IN MICROBIOLOGY
(2019)
Review
Environmental Sciences
Ingrid Chorus, Jutta Fastner, Martin Welker
Summary: Climate change may promote cyanobacterial blooms in certain waterbodies; controlling blooms and toxin concentrations require reducing phosphorus and nitrogen concentrations; health risks depend on whether concentrations exceed WHO cyanotoxin guideline values.
Editorial Material
Multidisciplinary Sciences
Jef Huisman, Elke Dittmann, Jutta Fastner, J. Merijn Schuurmans, J. Thad Scott, Dedmer B. Van de Waal, Petra M. Visser, Martin Welker, Ingrid Chorus
Summary: Hellweger et al. suggest that phosphorus limitation will lead to higher concentrations of cyanobacterial toxins in lakes. However, their assumptions about molecular, physiological, and ecological mechanisms lack support and contradict other studies. It is concluded that their take-home message about increased toxicity in Lake Erie due to phosphorus load reduction is fundamentally flawed.
Article
Environmental Sciences
Jutta Fastner, Jonna Teikari, Anja Hoffmann, Antje Kohler, Sebastian Hoppe, Elke Dittmann, Martin Welker
Summary: Fatal dog poisoning in Tegeler See, Berlin, Germany, caused by neurotoxic cyanobacteria associated with aquatic macrophytes has raised concerns about human exposure, especially for children, to cyanotoxins during recreational activities. A study conducted from 2017 to 2021 analyzed 398 macrophyte samples and 463 water samples from bathing sites in 19 Berlin lakes, detecting cyanotoxins in 66% of macrophyte samples and 50% of water samples. Anatoxins were the most frequently detected toxin group in macrophyte samples (58%), while cylindrospermopsins were most prevalent in water samples (41%). The study also identified the presence of anatoxin-producing cyanobacteria in the water moss Fontinalis antipyretica. Based on toxicological evaluations, a critical anatoxin content of 3 μg/g fresh weight in macrophyte samples is proposed.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)