期刊
SYSTEMATIC AND APPLIED MICROBIOLOGY
卷 33, 期 6, 页码 316-321出版社
ELSEVIER GMBH
DOI: 10.1016/j.syapm.2010.06.005
关键词
Taxonomy; Hydrocarbon biodegradation; Maldi-Tof; MLSA; Breoghania corrubedonensis; New genus
资金
- Spanish Ministry of Education and Science [VEM2004-08-556, CGL2007-64199/BOS]
- Generalitat de Catalunya
- Spanish Government [CE-CSD2007-0005, CLG2009_12651-C02-02]
- FEDER
A Gram-negative bacterium designated UBF-P1(T) was isolated from an enrichment culture established in nutrient supplemented artificial sea water with pyrene as a carbon source, and inoculated with a marine fuel oil-degrading consortium obtained from a sand sample collected from the beach of Corrubedo (A Coruna, Galicia, Spain) after the Prestige accidental oil spill. Phylogenetic analysis based on the almost complete 16S rRNA gene sequence affiliated strain UBF-P1(T) with the family Cohaesibacteraceae, Cohaesibacter gelatinilyticus (DSM 18289(T)) being the closest relative species with 92% sequence similarity. Cells were irregular rods, motile, strictly aerobic, catalase and oxidase positive. Ubiquinone 10 was the major respiratory lipoquinone. The major polar lipids comprised diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidylmonomethylethanolamine (PME), and phosphatidylcholine (PC). The major fatty acids detected were C-18:1 omega 7C, C-19:0 cyclo omega 8c, and C-16:0. The G + C content of strain UBF-P1(T) was 63.9 mol%. The taxonomic comparison with the closest relative based on genotypic, phenotypic and chemotaxonomic characteristics supported that strain UBF-P1(T) could be classified as a novel genus and species, for which the name Breoghania corrubedonensis gen. nov., sp. nov. is proposed. The type strain of this new taxon is UBF-P1(T) (CECT 7622, LMG 25482, DSM 23382). (C) 2010 Elsevier GmbH. All rights reserved.
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