期刊
SYSTEMATIC AND APPLIED MICROBIOLOGY
卷 32, 期 7, 页码 471-479出版社
ELSEVIER GMBH
DOI: 10.1016/j.syapm.2009.06.004
关键词
Aeromonas piscicola sp nov.; DNA-DNA hybridizations; 16S rDNA; Housekeeping genes
资金
- Ministerio de Ciencia y Tecnologia (Spain) [AGL2006-13208-C02-01]
Four Aeromonas strains (S1.2(T), EO-0505, TC1 and TI 1.1) isolated from moribund fish in Spain showed a restriction fragment length polymorphism (RFLP) pattern related to strains of Aeromonas salmonicida and Aeromonas bestiarum but their specific taxonomic position was unclear. Multilocus sequence analysis (MLSA) of housekeeping genes rpoD, gyrB, recA and dnaJ confirmed the allocation of these isolates to an unknown genetic lineage within the genus Aeromonas with A. salmonicida, A. bestiarum and Aeromonas popoffii as the phylogenetically nearest neighbours. Furthermore, a strain biochemically labelled as Aeromonas hydrophila (AH-3), showing a pattern of A. bestiarum based on 16S rDNA-RFLP, also clustered with the unknown genetic lineage. The genes rpoD and gyrB proved to be the best phylogenetic markers for differentiating these isolates from their neighbouring species. Useful phenotypic features for differentiating the novel species from other known Aeromonas species included their ability to hydrolyze elastin, produce acid from L-arabinose and salicin, and their inability to produce acid from lactose and use L-lactate as a sole carbon source. A polyphasic approach using phenotypic characterization, phylogenetic analysis of the 16S rRNA gene and of four housekeeping genes, as well as DNA-DNA hybridization studies and an analysis of the protein profiles by MALDI-TOF-MS, showed that these strains represented a novel species for which the name Aeromonas piscicola sp. nov. is proposed with isolate S1.2(T) ( = CECT 7443(T), LMG 24783(T)) as the type strain. (C) 2009 Elsevier GmbH. All rights reserved.
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