期刊
SYNTHETIC METALS
卷 169, 期 -, 页码 18-24出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.synthmet.2013.02.016
关键词
Polymer; Protein antibody; Immunosensor; Impedance
资金
- CSIR, India
We report structural and ac impedimetric properties of a biofunctionalized copolymer poly(pyrrole-co-pyrrolepropylic acid) (PPy-PPa) film electrochemically grown onto an indium-tin-oxide (ITO) coated glass plate. The copolymer film was bio-functionalized with myoglobin protein antibody, Ab-cMb, to form a bioelectrode. The bioelectrode was characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), contact angle measurement and electrochemical techniques. The ac impedance studies of the PPy-PPa copolymer film show both charge transfer resistance (R-et) and ions diffusion (W-R) characteristics, at high and low frequency regions respectively, whereas the biofunctionalized electrode shows only Ret in a comparatively low ac frequency region with respect to the PPy-PPa film, indicating a good biocompatibility of the polymer electrode. The ac impedimetric performance of the bioelectrode was studied toward the detection of myoglobin protein antigen, Ag-cMb, in a phosphate buffer solution. The bioelectrode exhibited a linear impedance response to Ag-cMb concentration in the range long to 1 mu g mL(-1). (C) 2013 Elsevier B.V. All rights reserved.
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