期刊
STRUCTURE
卷 20, 期 3, 页码 504-512出版社
CELL PRESS
DOI: 10.1016/j.str.2012.01.012
关键词
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资金
- Wellcome Trust
- Medical Research Council (UK)
- Human Frontier Science Programme
- Biotechnology and Biological Sciences Research Council
- Birkbeck College
- Papworth Hospital NHS Trust
- Medical Research Council [G0700990, MC_U117533887, G0901786, G0701236, MC_G1000734] Funding Source: researchfish
- MRC [MC_U117533887, G0901786, G0700990, MC_G1000734, G0701236] Funding Source: UKRI
In conformational diseases, native protein conformers convert to pathological intermediates that polymerize. Structural characterization of these key intermediates is challenging. They are unstable and minimally populated in dynamic equilibria that may be perturbed by many analytical techniques. We have characterized a forme fruste deficiency variant of alpha(1)-antitrypsin (Lys154Asn) that forms polymers recapitulating the conformer-specific neo-epitope observed in polymers that form in vivo. Lys154Asn alpha(1)-antitrypsin populates an intermediate ensemble along the polymerization pathway at physiological temperatures. Nuclear magnetic resonance spectroscopy was used to report the structural and dynamic changes associated with this. Our data highlight an interaction network likely to regulate conformational change and do not support the recent contention that the disease-relevant intermediate is substantially unfolded. Conformational disease intermediates may best be defined using powerful but minimally perturbing techniques, mild disease mutants, and physiological conditions.
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