期刊
STRUCTURE
卷 18, 期 7, 页码 777-786出版社
CELL PRESS
DOI: 10.1016/j.str.2010.06.001
关键词
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资金
- Swedish Research Council
- Wenner-Gren foundation
- Marie-Curie Early-Stage Training grant
We have developed methods for ab initio three-dimensional (3D) structure determination from projection images of randomly oriented single molecules coexisting in multiple functional states, to aid the study of complex samples of macromolecules and nanoparticles by electron microscopy (EM). New algorithms for the determination of relative 3D orientations and conformational state assignment of single-molecule projection images are combined with well-established techniques for alignment and statistical image analysis. We describe how the methodology arrives at homogeneous groups of images aligned in 3D and discuss application to experimental EM data sets of the Escherichia coli ribosome and yeast RNA polymerase II.
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