Article
Chemistry, Medicinal
Edward Price, J. Cory Kalvass, David DeGoey, Balakrishna Hosmane, Stella Doktor, Kelly Desino
Summary: This study evaluated different models for predicting human intestinal absorption data, providing a resource for DMPK scientists and medicinal/computational chemists to enhance their understanding of the utility and applications of permeability and absorption models for academia and industry.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Oncology
Lijuan Bai, Xin Li, Yang Yang, Rui Zhao, Elshaddai Z. White, Alira Danaher, Nathan J. Bowen, Cimona V. Hinton, Nicholas Cook, Dehong Li, Alyssa Y. Wu, Min Qui, Yuhong Du, Haian Fu, Omer Kucuk, Daqing Wu
Summary: Chemoresistance is a major obstacle in the clinical management of metastatic, castration-resistant prostate cancer. This study identified bromocriptine mesylate as a potent and selective inhibitor of chemoresistant PCa cells. Bromocriptine effectively induced cell cycle arrest and activated apoptosis in chemoresistant PCa cells but not in chemoresponsive PCa cells. RNA-seq analyses revealed that bromocriptine affected a subset of genes implicated in the regulation of cell cycle, DNA repair, and cell death.
TRANSLATIONAL ONCOLOGY
(2023)
Article
Microbiology
A. E. Marriott, J. L. Dagley, S. Hegde, A. Steven, C. Fricks, U. DiCosty, A. Mansour, E. J. Campbell, C. M. Wilson, F. Gusovsky, S. A. Ward, W. D. Hong, P. O'Neill, A. Moorhead, S. McCall, J. W. McCall, M. J. Taylor, J. D. Turner
Summary: We demonstrated that lymphopenic mouse strains like NSG and RAG2 are susceptible to the larval development phase of heartworm disease. These mouse models can provide a refined alternative for preclinical drug research and reduce the need for experimental use of cats or dogs.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Melica Nourmoussavi Brodeur, Kayla Simeone, Kim Leclerc-Deslauniers, Hubert Fleury, Euridice Carmona, Diane M. Provencher, Anne-Marie Mes-Masson
Summary: The research highlights the discrepancies in therapeutic response across different model systems, showing that the carboplatin response in EOC cell lines cultured in a 3D ex vivo model correlates best with the in vivo response. This suggests that the 3D ex vivo model may be a more feasible, reliable, and cost-effective preclinical model for drug screening and prediction studies in EOC.
SCIENTIFIC REPORTS
(2021)
Review
Oncology
Wout Devlies, Florian Handle, Gaetan Devos, Steven Joniau, Frank Claessens
Summary: This review investigates the existing methods to study treatment resistance to androgen receptor targeted therapies in prostate cancer, including the role of preclinical models and understanding of resistance mechanisms. Understanding resistance will greatly improve insights into tumor pathophysiology and future treatment strategies in prostate cancer.
Review
Oncology
Reihaneh Alsadat Mahmoudian, Moein Farshchian, Fatemeh Fardi Golyan, Parvaneh Mahmoudian, Ali Alasti, Vahid Moghimi, Mina Maftooh, Majid Khazaei, Seyed Mahdi Hassanian, Gordon A. Ferns, Hanie Mahaki, Soodabeh Shahidsales, Amir Avan
Summary: Preclinical models in cancer research can be manipulated to mimic human behavior and conditions through environmental, genomic, molecular, organ system, and physical activity modifications. Recent developments in genetic engineering, such as CRISPR/Cas9, transposable elements, epigenome modifications, and liquid biopsies, have advanced the creation of mouse models for studying cancer. This review focuses on the production and usage of xenografts, chemical-induced models, and genetically engineered mouse models (GEMMs), specifically for esophageal cancer research, and briefly discusses potential gene-editing tools.
CRITICAL REVIEWS IN ONCOLOGY HEMATOLOGY
(2023)
Review
Zoology
Sergey Karakashev, Ru-Gang Zhang
Summary: Epithelial ovarian cancer is the leading cause of gynecological cancer-related mortality in developed countries, with multiple histological and molecular subtypes. Research focusing on animal models that consider the heterogeneity of the disease is crucial for studying novel therapies.
ZOOLOGICAL RESEARCH
(2021)
Article
Biochemical Research Methods
Danielle Maeser, Robert F. Gruener, Rong Stephanie Huang
Summary: Cell line drug screening datasets can be used for various drug discovery applications, including correcting drug sensitivity levels, predicting clinical drug response, and associating predictions with clinical features for drug biomarker discovery. These methodologies have been unified and updated into the OncoPredict R package to facilitate tool development and adoption, applicable for drug and biomarker discovery in various in vitro and in vivo contexts.
BRIEFINGS IN BIOINFORMATICS
(2021)
Review
Oncology
Robert Adamiecki, Anita Hryniewicz-Jankowska, Maria A. Ortiz, Xiang Li, Baylee A. Porter-Hansen, Imad Nsouli, Gennady Bratslavsky, Leszek Kotula
Summary: This review examines the in vivo models of prostate cancer, specifically focusing on recently proposed mouse models. The comparison and analysis of these models provides researchers with valuable information to inform their future studies on prostate cancer. The review also highlights the importance of incorporating various types of models to enhance our understanding of prostate cancer.
Article
Biotechnology & Applied Microbiology
Alyna Katti, Adrian Vega-Perez, Miguel Foronda, Jill Zimmerman, Maria Paz Zafra, Elizabeth Granowsky, Sukanya Goswami, Eric E. Gardner, Bianca J. Diaz, Janelle M. Simon, Alexandra Wuest, Wei Luan, Maria Teresa Calvo Fernandez, Anastasia P. Kadina, John A. Walker II, Kevin Holden, Scott W. Lowe, Francisco J. Sanchez Rivera, Lukas E. Dow
Summary: To study the impact of single-nucleotide variants (SNVs) on tumor initiation and progression, researchers developed an inducible base editing (iBE) mouse model that allows for temporal and regulatable in vivo gene editing. This model efficiently edits SNVs in intestinal, lung, and pancreatic organoids using plasmid-based or synthetic guide RNAs. Temporal regulation of base editor activity allows for controlled sequential genome editing both ex vivo and in vivo, while delivery of sgRNAs directly to target tissues facilitates the generation of in situ preclinical cancer models. The iBE mouse model enables the testing of the function of SNVs.
NATURE BIOTECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Sherif Attalla, Tarek Taifour, Tung Bui, William Muller
Summary: Understanding breast cancer progression, metastasis, and therapeutic discovery is crucial. The MMTV-PyMT mouse model is a valuable tool that helps researchers better understand the molecular basis of breast cancer.
Review
Urology & Nephrology
Lucas Germain, Camille Lafront, Virginie Paquette, Bertrand Neveu, Jean-Sebastien Paquette, Frederic Pouliot, Etienne Audet-Walsh
Summary: Prostate cancer depends on androgen receptor (AR) for growth and survival, making it the main target for pharmacological treatment. However, prostate cancer eventually becomes castration-resistant, with most resistance mechanisms involving reactivation of AR. The definition of preclinical models and cell lines for studying prostate cancer and castration resistance is still unclear. Understanding the limitations and advantages of these models is crucial for effective translation of scientific knowledge to clinical applications. Evaluation: x/10
NATURE REVIEWS UROLOGY
(2023)
Review
Pharmacology & Pharmacy
Isha Karnik, Zhisheng Her, Shu Hui Neo, Wai Nam Liu, Qingfeng Chen
Summary: Cancer therapeutics have been extensively studied in the past decade, with a focus on targeted modalities and immunotherapy. The adverse side effects of immune checkpoint inhibitors have underlined the need for clinically relevant animal models. Humanized mouse models have emerged as valuable tools for pre-clinical research, enabling the evaluation of efficacy and safety of immunotherapy, as well as the investigation of novel disease mechanisms.
Article
Biotechnology & Applied Microbiology
Arjanneke F. van de Merbel, Geertje van der Horst, Maaike H. van der Mark, Selas T. F. Bots, Diana J. M. van den Wollenberg, Corrina M. A. de Ridder, Debra Stuurman, Tilly Aalders, Sigrun Erkens-Schulz, Nadine van Montfoort, Wouter R. Karthaus, Niven Mehra, Minke Smits, Jack A. Schalken, Wytske M. van Weerden, Rob C. Hoeben, Gabri van der Pluijm
Summary: The study evaluated the oncolytic efficacy of the bioselected oncolytic reovirus mutant jin-3 in multiple human prostate cancer models, showing tumor tropism and potent anti-cancer immune responses.
CANCER GENE THERAPY
(2022)
Article
Oncology
Xin Li, Yanhua Chen, Lijuan Bai, Rui Zhao, Yifei Wu, Zhong-Ru Xie, Jason M. M. Wu, Nathan J. Bowen, Alira Danaher, Nicholas Cook, Dehong Li, Min Qui, Yuhong Du, Haian Fu, Adeboye O. Osunkoya, Omer Kucuk, Daqing Wu
Summary: Nicardipine exhibited high selectivity and potency against chemoresistant PCa cells by inducing apoptosis and cell cycle arrest. It inhibited key components of noncanonical EZH2 signaling pathway, thus improving the clinical efficacy of chemoresistant prostate cancer.
BRITISH JOURNAL OF CANCER
(2023)
Article
Environmental Sciences
Lijian Han, Xiaoyan Zhang, Weiqi Zhou, Miaogen Shen, Yan Huang, Weifeng Li, Yuguo Qian
Summary: In recent years, rapid urbanization in China has significantly impacted the eco-environment, with studies showing a correlation between urban expansion and population-weighted vegetation and PM2.5 concentration. Different periods of urban expansion have demonstrated varying trends in population-weighted vegetation and PM2.5 concentration.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Nursing
M. D. Shen, Y. W. Li, L. Q. Xu, H. Y. Shi, Y. Y. Ni, H. J. Lin, F. Li
Summary: The study aimed to investigate the effect of active cycle of breathing technique on sputum viscosity and production among patients with chronic obstructive pulmonary disease. The results showed significant improvements in respiratory function and sputum production with the intervention.
INTERNATIONAL JOURNAL OF NURSING STUDIES
(2021)
Article
Optics
Yuntao Xu, Mohan Shen, Juanjuan Lu, Joshua B. Surya, Ayed Al Sayem, Hong X. Tang
Summary: This study demonstrates that the dielectric cladding on thin-film lithium niobate microring resonators has a significant impact on the photorefractive effect, and removing the dielectric cladding layer can effectively alleviate this effect. This research offers a reliable method for controlling the photorefractive effect on thin-film lithium niobate, which will further enhance the performance of integrated classical and quantum photonic devices based on thin-film lithium niobate.
Article
Environmental Sciences
Dailiang Peng, Yan Wang, George Xian, Alfredo R. Huete, Wenjiang Huang, Miaogen Shen, Fumin Wang, Le Yu, Liangyun Liu, Qiaoyun Xie, Lingling Liu, Xiaoyang Zhang
Summary: Shrublands cover 13% of the global land surface, but current satellite-derived land surface phenology products lack detection in some semi-arid areas. Research shows that green-up onset date shifts to earlier dates with increasing vegetation cover, becoming fully detectable at over 50% vegetation cover. This highlights the importance of considering vegetation cover when monitoring and assessing phenological dynamics in shrubland ecosystems.
REMOTE SENSING OF ENVIRONMENT
(2021)
Article
Environmental Sciences
Lei Zhang, Miaogen Shen, Chunming Shi, Fangzhong Shi, Nan Jiang, Zhiyong Yang, Zhenming Ji
Summary: In the Tibetan Plateau, large-scale climate oscillations, such as the Atlantic Multidecadal Oscillation (AMO) and the Pacific Decadal Oscillation (PDO), can indirectly impact vegetation growth by modifying local climatic factors differently across regions.
FRONTIERS IN ENVIRONMENTAL SCIENCE
(2021)
Article
Oncology
Weiping Li, Michael M. Shen
Summary: Prostate adenocarcinoma lacks distinguishable histopathological subtypes, but prostate cancer displays significant inter- and intratumor heterogeneity at the molecular level. Understanding the basis for prostate cancer heterogeneity can help distinguish aggressive from indolent disease, and help overcome treatment resistance in advanced prostate cancer.
SEMINARS IN CANCER BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Jennifer Cable, Duanqing Pei, Lola M. Reid, Xin Wei Wang, Sonam Bhatia, Panagiotis Karras, Jan Joseph Melenhorst, Markus Grompe, Justin D. Lathia, Erwei Song, Calvin J. Kuo, Ning Zhang, Richard M. White, Stephanie Ky Ma, Lichun Ma, Y. Rebecca Chin, Michael M. Shen, Irene Oi Lin Ng, Klaus H. Kaestner, Lei Zhou, Shaheen Sikandar, Clemens A. Schmitt, Wei Guo, Carmen Chak-Lui Wong, Junfang Ji, Dean G. Tang, Anna Dubrovska, Chunzhang Yang, Wolf R. Wiedemeyer, Irving L. Weissman
Summary: The test for the cancer stem cell hypothesis is to find a target expressed uniquely on CSCs in a patient's tumor and eliminate them to eradicate cancer, which has not yet been achieved. However, progress in CSC diagnostics, targeting, and understanding the importance of eliminating tumor cells with self-renewal properties has led to clinically relevant therapies. Recent research suggests that targeting CSCs may offer novel therapeutic strategies with clinical applicability.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2021)
Article
Oncology
Laura Crowley, Michael M. Shen
Summary: The recent advancement in single-cell RNA-sequencing technology has provided new insights into the heterogeneity of prostate epithelium. Studies have shown extensive heterogeneity among luminal epithelial cells in different regions of the prostate, with varying progenitor potentials and plasticity in context-dependent environments.
Article
Oncology
Veronica Gil, Susana Miranda, Ruth Riisnaes, Bora Gurel, Mariantonietta D'Ambrosio, Alessandro Vasciaveo, Mateus Crespo, Ana Ferreira, Daniela Brina, Martina Troiani, Adam Sharp, Beshara Sheehan, Rossitza Christova, George Seed, Ines Figueiredo, Maryou Lambros, David Dolling, Jan Rekowski, Abdullah Alajati, Matthew Clarke, Rita Pereira, Penny Flohr, Gemma Fowler, Gunther Boysen, Semini Sumanasuriya, Diletta Bianchini, Pasquale Rescigno, Caterina Aversa, Nina Tunariu, Christina Guo, Alec Paschalis, Claudia Bertan, Lorenzo Buroni, Jian Ning, Suzanne Carreira, Paul Workman, Amanda Swain, Andrea Califano, Michael M. Shen, Andrea Alimonti, Antje Neeb, Jonathan Welti, Wei Yuan, Johann de Bono
Article
Biochemistry & Molecular Biology
Francesco Cambuli, Veronica Foletto, Alessandro Alaimo, Dario De Felice, Francesco Gandolfi, Maria Dilia Palumbieri, Michela Zaffagni, Sacha Genovesi, Marco Lorenzoni, Martina Celotti, Emiliana Bertossio, Giosue Mazzero, Arianna Bertossi, Alessandra Bisio, Francesco Berardinelli, Antonio Antoccia, Marco Gaspari, Mattia Barbareschi, Michelangelo Fiorentino, Michael M. Shen, Massimo Loda, Alessandro Romanel, Andrea Lunardi
Summary: This study demonstrates that non-canonical Activin A signaling plays a critical role in maintaining epithelial quiescence in the healthy prostate, with potential implications for understanding cancer initiation and the development of therapies targeting quiescent tumor progenitors.
Article
Medicine, Research & Experimental
Alberto Bartolome, Nina Suda, Junjie Yu, Changyu Zhu, Jinsook Son, Hongxu Ding, Andrea Califano, Domenico Accili, Utpal B. Pajvani
Summary: This study suggests that Notch/Ephrin signaling can permanently alter islet architecture during a morphogenetic window in early life, leading to beta cell dysfunction and progression of type 2 diabetes (T2D).
Article
Health Care Sciences & Services
Paula Peremiquel-Trillas, Sonia Paytubi, Beatriz Pelegrina, Jon Frias-Gomez, Alvaro Carmona, Jose Manuel Martinez, Javier de Francisco, Yolanda Benavente, Marc Barahona, Ferran Brianso, Julia Canet-Hermida, Victor Cano, August Vidal, Alba Zanca, Nuria Baixeras, Axel Rodriguez, Sergi Fernandez-Gonzalez, Nuria Duenas, Laura Cardenas, Alvaro Aytes, Ilaria Bianchi, Miquel Angel Pavon, Jaume Reventos, Gabriel Capella, David Gomez, Mireia Diaz, Jordi Ponce, Joan Brunet, Xavier Matias-Guiu, Francesc Xavier Bosch, Silvia de Sanjose, Laia Alemany, Marta Pineda, Fatima Marin, Laura Costas
Summary: Screenwide is a four-year case-control study aimed at evaluating potential risk factors for endometrial and ovarian cancers, as well as developing early detection strategies. The results of the study showed that genetic variants in the samples could be detected through various methods for early detection, suggesting the potential application of this approach in clinical practice.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Biology
Yizhuo Zhou, Ying Yang, Lihao Guo, Jun Qian, Jian Ge, Debora Sinner, Hongxu Ding, Andrea Califano, Wellington Cardoso
Summary: Using single cell RNA-sequencing and functional approaches, researchers identified six subpopulations of basal cells in the adult murine trachea. These subpopulations showed distinct gene expression signatures and functional differences, including their ability to self-renew and differentiate in response to environmental changes and injury. The heterogeneity of basal cells is established during embryonic development and depends on the integrity of local niches. The study also found that similar subpopulations exist in human airways.
Article
Cell Biology
Rasmani Hazra, Lily Brine, Libia Garcia, Brian Benz, Napon Chirathivat, Michael M. Shen, John Erby Wilkinson, Scott K. Lyons, David L. Spector
Summary: This study demonstrates that the lncRNA Platr4 directly influences cardiac mesoderm lineage differentiation and acts as a molecular scaffold interacting with molecules in the Hippo signaling pathway to regulate the expression of a key gene involved in cardiac lineage programming. Knockout of Platr4 in mice results in myocardial atrophy and valve mucinous degeneration, which are associated with reduced cardiac output and sudden heart failure.
DEVELOPMENTAL CELL
(2022)
Article
Multidisciplinary Sciences
Zhengping Shao, Brian J. Lee, Hanwen Zhang, Xiaohui Lin, Chen Li, Wenxia Jiang, Napon Chirathivat, Steven Gershik, Michael M. Shen, Richard Baer, Shan Zha
Summary: PARP1 is an important enzyme recruited and activated by DNA strand breaks to maintain genomic stability. However, the expression of catalytically inactive PARP1 negatively affects murine embryonic development, providing a mechanism for the side effects of PARP inhibitors used for cancer therapy.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
