Article
Biochemistry & Molecular Biology
Chiemi Kimura-Nakajima, Kousuke Sakaguchi, Yoshiko Hatano, Masahito Matsumoto, Yasushi Okazaki, Keisuke Tanaka, Takumi Yamane, Yuichi Oishi, Kenji Kamimoto, Ken Iwatsuki
Summary: The authors found through experimental research that Ngn3-positive endocrine precursor cells in adult mice may arise from the pancreatic duct, indicating that the pancreatic duct could be a potential donor for therapeutic use.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Tessa Dignum, Barbara Varnum-Finney, Sanjay R. Srivatsan, Stacey Dozono, Olivia Waltner, Adam M. Heck, Takashi Ishida, Cynthia Nourigat-McKay, Dana L. Jackson, Shahin Rafii, Cole Trapnell, Irwin D. Bernstein, Brandon Hadland
Summary: This study demonstrates that rare HE with functional HSC potential in the early murine embryo are distinct from more abundant HE with multilineage hematopoietic potential that fail to generate HSCs. HSC-competent HE are characterized by expression of CXCR4 surfacemarker and higher expression of genes tied to arterial programs regulating HSC dormancy and self-renewal.
Article
Biochemistry & Molecular Biology
Xiaorui Geng, Jinmei Xue, Haoyue Zheng, Limin Suo, Haotao Zeng, Miao Zhao, Shuo Song, Yu Liu, Changqing Zhao, Pingchang Yang
Summary: CD46 contributes to the production of IgE and its activation may be involved in the pathogenesis of allergic diseases. This study found increased CD46 expression in peripheral B cells of patients with allergic rhinitis (AR), which was positively associated with the AR response. Ablation of CD46 in B cells prevented the production of IgE in mice with airway allergy, and exposure to aluminum hydroxide upregulated Cd46 expression. Administration of Cd46 shRNA nanoparticles attenuated experimental airway allergy.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Multidisciplinary Sciences
Nicholas J. Hess, David P. Turicek, Jeremiah Riendeau, Sean J. McIlwain, Emmanuel Contreras Guzman, Kalyan Nadiminti, Amy Hudson, Natalie S. Callander, Melissa C. Skala, Jenny E. Gumperz, Peiman Hematti, Christian M. Capitini
Summary: An important aspect of allogeneic hematopoietic cell transplantations is finding a way to prevent graft-versus-host disease (GVHD) while still maintaining the graft-versus-leukemia (GVL) activity of donor T cells. In an observational clinical study, a specific type of T cell called CD4+/CD8+ double-positive T cell (DPT) was identified in recipients of allo-HCT, and its presence was found to be predictive of severe GVHD. Further experiments using a transplant model revealed that DPTs develop from antigen-stimulated CD8 T cells and exhibit distinct characteristics from single-positive CD4 and CD8 T cells. These findings highlight the role of DPTs in GVHD pathology.
Review
Allergy
Pattraporn Satitsuksanoa, Monique Daanje, Mubeccel Akdis, Scott D. Boyd, Willem van de Veen
Summary: B cells play a crucial role in the development of IgE-mediated food allergies by producing allergen-specific antibodies, but the mechanisms regulating allergen-specific IgE production and immunological memory are not fully understood. Recent technological advances have allowed detailed analysis of B cells at a single-cell level, providing a better understanding of their role in food allergies.
Article
Biochemistry & Molecular Biology
Aleksandra Rodak, Katharina Stadlbauer, Madhusudhan Reddy Bobbili, Oskar Smrzka, Florian Rueker, Gordana Wozniak Knopp
Summary: This study developed an antibody-drug conjugate that showed cytotoxicity for IgE-positive cells. The antibody had fast internalization and strict target specificity, providing a valuable starting point for the development of an anti-IgE cell-depleting agent.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Neta Gurwicz, Liat Stoler-Barak, Niklas Schwan, Arnab Bandyopadhyay, Michael Meyer-Hermann, Ziv Shulman
Summary: This study reveals that Tingible body macrophages (TBMs) originate from lymph node-resident precursors and consume germinal center B cells through dynamic protrusions to prevent intracellular-content dissemination. The dynamics and function of TBMs provide insights into the emergence of various antibody-mediated autoimmune conditions.
JOURNAL OF EXPERIMENTAL MEDICINE
(2023)
Review
Pediatrics
Chioma Udemgba, Adora Lin
Summary: B cells play important roles in the development of food allergy and tolerance. Understanding B cell biology in the context of food allergy is crucial for effective prevention and therapy strategies. Allergen-specific IgG4 and regulatory B cells may be key components in promoting tolerance and protecting against food allergies.
CURRENT OPINION IN PEDIATRICS
(2021)
Article
Multidisciplinary Sciences
Yaara Oren, Michael Tsabar, Michael S. Cuoco, Liat Amir-Zilberstein, Heidie F. Cabanos, Jan-Christian Hutter, Bomiao Hu, Pratiksha I. Thakore, Marcin Tabaka, Charles P. Fulco, William Colgan, Brandon M. Cuevas, Sara A. Hurvitz, Dennis J. Slamon, Amy Deik, Kerry A. Pierce, Clary Clish, Aaron N. Hata, Elma Zaganjor, Galit Lahav, Katerina Politi, Joan S. Brugge, Aviv Regev
Summary: Recent studies have revealed that cancer cells can enter different lineages in response to treatment, with distinct biological characteristics and metabolic programs. Proliferative capacity of drug-tolerant persister cells is associated with upregulation of antioxidant gene programs and a metabolic shift to fatty acid oxidation.
Article
Biology
Julia Schanin, Wouter Korver, Emily C. Brock, John Leung, Zachary Benet, Thuy Luu, Katherine Chang, Alan Xu, Naomi De Freitas, Kenneth Luehrsen, Michael A. Brehm, Alan Wong, Bradford A. Youngblood
Summary: This study identified an agonistic Siglec-6 mAb that induces inhibition and reduction of mast cells through Fc-mediated interaction. The findings suggest that targeting Siglec-6 with agonistic antibodies may be a potential therapeutic approach for allergic diseases.
COMMUNICATIONS BIOLOGY
(2022)
Article
Microbiology
Rajesh Balagam, Pengbo Cao, Govind P. Sah, Zhaoyang Zhang, Kalpana Subedi, Daniel Wall, Oleg A. Igoshin
Summary: This study sheds light on the mechanism of emergent social behavior in Myxococcus xanthus through overexpression of TraA and TraB adhesins. Both experiments and mathematical modeling demonstrate the ability to predict self-organization patterns even when phenotypically distinct strains are mixed.
Article
Multidisciplinary Sciences
Hector Cordero, Rodney G. King, Pranay Dogra, Chloe Dufeu, Sarah B. See, Alexander M. Chong, Anne-Catrin Uhlemann, Siu-Hong Ho, David M. Kalfa, Emile A. Bacha, John F. Kearney, Emmanuel Zorn
Summary: The thymus of human neonates contains CD138(+) plasma cells that produce antibodies against bacteria, serving as a source of innate humoral immunity in human neonates.
NATURE COMMUNICATIONS
(2021)
Review
Chemistry, Multidisciplinary
Marco Bertolini, Man Sing Wong, Lorena Mendive-Tapia, Marc Vendrell
Summary: T cells play crucial roles in adaptive response and tissue homeostasis in the immune system. Smart probes, including small molecule fluorophores and nanoconstructs, have been developed to image T cells in tumors and inflammation sites by targeting metabolic and enzymatic biomarkers as well as specific surface receptors. This review summarizes recent advancements in the design, synthesis, and application of smart probes for imaging T cells, and discusses their use in monitoring T cell response to anti-cancer immunotherapies.
CHEMICAL SOCIETY REVIEWS
(2023)
Article
Multidisciplinary Sciences
Weizhen Jia, Lingyu Kong, Hiroyasu Kidoya, Hisamichi Naito, Fumitaka Muramatsu, Yumiko Hayashi, Han-Yun Hsieh, Daishi Yamakawa, Daniel K. Hsu, Fu-Tong Liu, Nobuyuki Takakura
Summary: This study demonstrates that Gal-3 expression in long-term repopulating HSCs is induced in response to Tie2 and Mpl, and plays a crucial role in maintaining HSC quiescence through regulation of p21.
NATURE COMMUNICATIONS
(2021)
Review
Allergy
Paul Engeroff, Monique Vogel
Summary: IgE can bind to two receptors, playing different roles on different cells, inducing allergic reactions on one hand and participating in IgE regulation and antigen presentation on the other. In inflammation and immune homeostasis, CD23 plays an important modulatory role.
Article
Cell Biology
Mathieu Lesouhaitier, Murielle Gregoire, Arnaud Gacouin, Valentin Coirier, Aurelien Frerou, Caroline Piau, Vincent Cattoir, Erwan Dumontet, Matthieu Revest, Pierre Tattevin, Antoine Roisne, Jean-Philippe Verhoye, Erwan Flecher, Yves Le Tulzo, Karin Tarte, Jean-Marc Tadie
Summary: This study aimed to investigate the impact of cardiopulmonary bypass (CPB) on neutrophil functions. The results showed that CPB induced a systemic inflammation with an increase in circulating mature neutrophils after surgery. Neutrophil lifespan was significantly increased and functions such as NET release and ROS production were enhanced after CPB, while bacteria killing and phagocytosis were not impacted. These data suggest that CPB recruits mature neutrophils via a demargination process and neutrophils remain efficient post-CPB without contributing to immunosuppression.
JOURNAL OF LEUKOCYTE BIOLOGY
(2022)
Article
Allergy
Anne Marchalot, Catherine Horiot, Jean-Marie Lambert, Claire Carrion, Christelle Oblet, Justine Pollet, Michel Cogne, Jeanne Moreau, Brice Laffleur, Laurent Delpy
Summary: This study demonstrates the effectiveness of an antisense oligonucleotide (ASO) strategy in reducing IgE secretion and promoting allergen tolerance by inducing apoptosis of IgE1 antibody-secreting cells.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2022)
Article
Urology & Nephrology
Vincent Javaugue, Virginie Pascal, Sebastien Bender, Sarah Nasraddine, Mathilde Dargelos, Mehdi Alizadeh, Alexis Saintamand, Matthieu Filloux, Paco Derouault, Sabrina Bouyer, Estelle Desport, Arnaud Jaccard, Frank Bridoux, Michel Cogne, Christophe Sirac
Summary: The study developed a high throughput sequencing assay to detect subtle clonal disorders in monoclonal gammopathy of renal significance, which could potentially improve the management of this kidney disease.
KIDNEY INTERNATIONAL
(2022)
Article
Multidisciplinary Sciences
Florian Reizine, Murielle Gregoire, Mathieu Lesouhaitier, Valentin Coirier, Juliette Gauthier, Celine Delaloy, Elise Dessauge, Florent Creusat, Fabrice Uhel, Arnaud Gacouin, Frederic Dessauge, Cecile Le Naoures, Caroline Moreau, Claude Bendavid, Yoann Daniel, Kilian Petitjean, Valerie Bordeau, Claire Lamaison, Caroline Piau, Vincent Cattoir, Mikael Roussel, Bernard Fromenty, Christian Michelet, Yves Le Tulzo, Jaroslaw Zmijewski, Ronan Thibault, Michel Cogne, Karin Tarte, Jean-Marc Tadie
Summary: Severe sepsis can lead to sustained immune dysfunction, resulting in organ failure, secondary infections, and late mortality. Supplementation with arginine or citrulline can restore T cell function and reduce immunosuppression induced by sepsis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Allergy
M. Cogne, Z. Dalloul, O. Deze, M. Le Goff, S. Thomas, M. Cahen, N. Ueda, B. Laffleur
Summary: Persisting adaptive humoral immunity relies on memory B lymphocytes and long-lived plasma cells. IgE responses, although protective against parasites and certain venoms, can have detrimental effects and result in chronic conditions with dramatic acute adverse events.
REVUE FRANCAISE D ALLERGOLOGIE
(2022)
Letter
Biochemistry & Molecular Biology
Diana Molino, Christine Durier, Anne Radenne, Corinne Desaint, Jacques Ropers, Soizic Courcier, Louis Victorien Vieillard, Claire Rekacewicz, Beatrice Parfait, Victor Appay, Frederic Batteux, Emmanuel Barillot, Michel Cogne, Behazine Combadiere, Christiane S. Eberhardt, Guy Gorochov, Philippe Hupe, Laetitia Ninove, Stephane Paul, Isabelle Pellegrin, Sylvie van der Werf, Maeva Lefebvre, Elisabeth Botelho-Nevers, Inmaculada Ortega-Perez, Marie Jaspard, Samba Sow, Jean Daniel Lelievre, Xavier de Lamballerie, Marie Paule Kieny, Eric Tartour, Odile Launay
Article
Immunology
Amelie Bos, Esil Aleyd, Lydia P. E. van der Steen, P. J. Winter, Niels Heemskerk, Stephan M. Pouw, Louis Boon, Rene J. P. Musters, Jantine E. Bakema, Cassian Sitaru, Michel Cogne, Marjolein van Egmond
Summary: IgA autoantibodies may play a role in autoimmune or chronic inflammatory diseases. A novel IgA autoimmune blistering model using genetically modified mice showed that IgA anti-collagen XVII could activate neutrophils and cause tissue damage, while treatment with anti-Fc alpha RI monoclonal antibodies prevented and resolved the disease.
FRONTIERS IN IMMUNOLOGY
(2022)
Editorial Material
Immunology
Michel Cogne, Thierry Fest, Said Aoufouchi
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Ophelie Deze, Brice Laffleur, Michel Cogne
Summary: Mature B cells utilize class switch recombination (CSR) to diversify immunoglobulin (Ig) production, facilitated by activation-induced deaminase (AID) targeting cytosines in transcribed targeted S regions. G-rich sequences in S regions form canonical G-quadruplexes (G4s) DNA structures, favoring CSR. Small molecules interacting with G4-DNA (G4 ligands) can regulate CSR either positively or negatively. The specific regulatory role of G4 structures in CSR and lymphomagenesis remains a major issue in understanding immune responses.
Article
Immunology
Lamine Alaoui, Javiera Villar, Renaud Leclere, Simon Le Gallou, Francis Relouzat, Henri-Alexandre Michaud, Karin Tarte, Natacha Teissier, Benoit Favier, Mikael Roussel, Elodie Segura
Summary: This study characterizes the phenotype, transcriptome, life cycle, and function of human tonsil macrophages, and identifies three subtypes with distinct features. CD36(hi) macrophages are related to monocytes, CD36(lo) macrophages have features of embryonic origin, and CD36(int) macrophages display mixed characteristics. Functionally, CD36(hi) macrophages are specialized in stimulating T follicular helper cells by producing Activin A. Stromal cell-derived TNF-alpha is identified as the inducer of Activin A secretion.
JOURNAL OF EXPERIMENTAL MEDICINE
(2023)
Article
Cell & Tissue Engineering
Severine Loisel, Pauline Lansiaux, Delphine Rossille, Cedric Menard, Joelle Dulong, Celine Monvoisin, Nadege Bescher, Isabelle Bezier, Maelle Latour, Audrey Cras, Dominique Farge, Karin Tarte
Summary: Initial overexpression of profibrotic factors by B cells is associated with a lack of clinical response to MSCs in severe SSc patients. Increase of regulatory B cells is a marker of clinical response to MSCs in SSc patients. MSCs directly upregulate IL-10 production by activated B cells in vitro.
STEM CELLS TRANSLATIONAL MEDICINE
(2023)
Article
Immunology
Nicolas Denis-Lagache, Christelle Oblet, Tiffany Marchiol, Audrey Baylet, Ophelie Teteau, Iman Dalloul, Zeinab Dalloul, Lina Zawil, Ophelie Deze, Jeanne Cook-Moreau, Alexis Saintamand, Hend Boutouil, Ahmed Amine Khamlichi, Claire Carrion, Sophie Peron, Sandrine Le Noir, Brice Laffleur, Michel Cogne
Summary: This study investigates the deletion of a specific gene structure that is associated with antibody gene variation and class switching, using a new mouse model. The results suggest that this gene deletion is associated with an increase in self-reactive antibodies.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Virginie Pascal, Marine Dupont, Paco de Rouault, David Rizzo, Delphine Rossille, Robin Jeannet, Thomas Daix, Bruno Francois, Steve Genebrier, Marie Cornic, Guillaume Monneret, Fabienne Venet, Juliette Ferrant, Mikael Roussel, Florian Reizine, Mathieu Le Souhaitier, Jean-Marc Tadie, Kartin Tarte, Jean Feuillard, Michel Cogne
Summary: To investigate the immune response architecture in COVID-19 patients and understand the factors that can cause or prevent ARDS, we analyzed the B cell responses using flow cytometry and Ig repertoire analysis. Flow cytometry with FlowSOM analysis revealed changes associated with COVID-19 inflammation, including an increase in double-negative B cells and ongoing plasma cell differentiation. We also found the expansion of two disconnected B cell repertoires. Analysis of Ig repertoire patterns showed an early expansion of IgG1 clonotypes with atypical CDR3 characteristics, which correlated with ARDS severity. Additionally, we observed a convergent response with progressively increasing somatic hypermutation and normal or short CDR3 in anti-SARS-CoV-2 clonotypes, which persisted until the memory B cell stage after recovery.
Article
Biochemistry & Molecular Biology
Ophelie Deze, Delfina Ordanoska, Delphine Rossille, Emma Miglierina, Brice Laffleur, Michel Cogne
Summary: Ig genes have the unique ability to be reshaped in B lymphocytes after encountering specific antigens, resulting in improved affinity and antibody production. Ig class switching, an important change in this process, is mediated by DNA repeats and DNA lesions. The discovery of IgH-like DNA repeats in the human IgH locus suggests their regulatory role in antibody production.
Article
Hematology
Martin Dreyling, Marc Andre, Nicola Gokbuget, Herve Tilly, Mats Jerkeman, John Gribben, Andres Ferreri, Pierre Morel, Stephan Stilgenbauer, Christopher Fox, Jose Maria Ribera, Sonja Zweegman, Igor Aurer, Csaba Bodor, Birgit Burkhardt, Christian Buske, Maria Dollores Caballero, Elias Campo, Bjoern Chapuy, Andrew Davies, Laurence de Leval, Jeanette Doorduijn, Massimo Federico, Philippe Gaulard, Francesca Gay, Paolo Ghia, Kirsten Gronbaek, Hartmut Goldschmidt, Marie-Jose Kersten, Barbara Kiesewetter, Judith Landman-Parker, Steven Le Gouill, Georg Lenz, Sirpa Leppa, Armando Lopez-Guillermo, Elizabeth Macintyre, Maria Victoria Mateos Mantega, Philippe Moreau, Carol Moreno, Bertrand Nadel, Jessica Okosun, Roger Owen, Sarka Pospisilova, Christiane Pott, Tadeusz Robak, Michelle Spina, Kostas Stamatopoulos, Jan Stary, Karin Tarte, Allessandra Tedeschi, Catherine Thieblemont, Ralf Ulrich Trappe, Lorenz H. Trumper, Gilles Salles