Article
Biology
Xiao Su, Xiaolei Li, Shiqing Wang, Xiaotong Xue, Rui Liu, Xiaojing Bai, Pixia Gong, Chao Feng, Lijuan Cao, Tingting Wang, Yayun Ding, Junjie Jiang, Yongjing Chen, Yufang Shi, Changshun Shao
Summary: This study found that Nestin-positive mesenchymal stromal cells (T-MSCs) mainly distribute in the medulla and cortical-medulla junction of the thymus, and possess immunosuppressive ability. Additionally, T-MSCs exhibit remarkable therapeutic efficacy in acute liver injury and inflammatory bowel disease (IBD).
Article
Immunology
Nicole Labusek, Yanis Mouloud, Christian Koester, Eva Diesterbeck, Tobias Tertel, Constanze Wiek, Helmut Hanenberg, Peter A. Horn, Ursula Felderhoff-Mueser, Ivo Bendix, Bernd Giebel, Josephine Herz
Summary: By comparing the neuroprotective effects of EVs derived from primary MSCs and ciMSCs in a mouse model of brain injury, we found that ciMSC-EVs can effectively reduce neuroinflammation and promote neuroregeneration, showing similar neuroprotective effects as primary MSC-EVs. This suggests that ciMSCs are an ideal cell source for the scaled manufacturing of EV-based therapeutics to treat neonatal and possibly also adult brain injury, overcoming the heterogeneity issue of MSCs.
INFLAMMATION AND REGENERATION
(2023)
Article
Cell & Tissue Engineering
Carolina Pradenas, Noymar Luque-Campos, Karina Oyarce, Rafael Contreras-Lopez, Felipe A. Bustamante-Barrientos, Andres Bustos, Felipe Galvez-Jiron, Maria Jesus Araya, Catalina Asencio, Raul Lagos, Yeimi Herrera-Luna, Daouda Abba Moussa, Charlotte Nicole Hill, Eliana Lara-Barba, Claudia Altamirano, Alexander Ortloff, Yessia Hidalgo-Fadic, Ana Maria Vega-Letter, Maria de los angeles Garcia-Robles, Farida Djouad, Patricia Luz-Crawford, Roberto Elizondo-Vega
Summary: This study reveals that lactate can act as a functional suppressive molecule and contributes to the enhanced immunosuppressive properties of glycolytic MSCs.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Veterinary Sciences
Lynn Pezzanite, Lyndah Chow, Gregg Griffenhagen, Steven Dow, Laurie Goodrich
Summary: This study compared the immunomodulatory and antibacterial properties of equine mesenchymal stromal cells (MSCs) cultured in different serum sources. Results showed that MSCs cultured in FBS exhibited improved functionality compared to equine serum, while there were no significant differences between cells cultured in autologous vs. allogeneic equine serum.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Sandhya Moise, Luigi Dolcetti, Francesco Dazzi, Paul Roach, Lee Buttery, Sheila MacNeil, Nick Medcalf
Summary: This study evaluated the immunosuppressive effect of alginate-encapsulated MSCs on proliferating mouse splenocytes. The results showed that MSCs significantly suppressed splenocyte proliferation via both direct and indirect mechanisms. Additionally, the study demonstrated for the first time that MSCs can sustain immunosuppressive activity when physically isolated from splenocytes.
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Oliwia Milek, Dino Tur, Lucia Ahcin, Olha Voitseshyna, Christian Behm, Oleh Andrukhov
Summary: This study investigated the impact of osteogenic differentiation on the immunomodulatory properties of hPDL-MSCs, and found that osteogenic differentiation reduced the ability of hPDL-MSCs to suppress CD4(+) T lymphocyte proliferation and enhanced this ability in the presence of IL-1 beta. Additionally, the expression and activity of indoleamine-2,3-dioxygenase and interleukin-8 were also affected by the differentiation process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Charindu K. I. Ranmuthu, Chanuka D. S. Ranmuthu, Chalukya K. Wijewardena, Matthew K. T. Seah, Wasim S. Khan
Summary: This systematic review explores the effect of hypoxia on chondrogenic differentiation of human adult mesenchymal stromal cells (MSCs). Most studies suggest that hypoxia during the expansion or differentiation phase enhances some markers of chondrogenic differentiation in adult MSCs. However, results are inconsistent and not always confirmed at the protein level. Factors such as MSC source, donor variability, hypoxia level, exposure time, and culture methodology contribute to these inconsistent findings.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Jie Zhang, Jiefang Huang, Yuting Gu, Mingxing Xue, Fengtao Qian, Bei Wang, Wanlin Yang, Hongshuang Yu, Qiwei Wang, Xin Guo, Xinyuan Ding, Jina Wang, Min Jin, Yanyun Zhang
Summary: The study revealed that chaperone-mediated autophagy (CMA) in mesenchymal stromal cells (MSCs) is inhibited in response to proinflammatory cytokines, affecting their immunosuppressive function. Inhibition of CMA led to enhanced expression of CXCL10 and iNOS in MSCs, promoting recruitment of inflammatory cells and subsequent inhibition of T cell proliferation through NF-kappa B and STAT1 activation. Additionally, AKT activation induced by IFN-gamma plus TNF-alpha played a role in CMA inhibition in MSCs.
CELLULAR & MOLECULAR IMMUNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Tim Ruhl, Corina Corsten, Justus P. Beier, Bong-Sung Kim
Summary: The study found that endocannabinoids could reduce cytokine secretion in macrophages and mesenchymal stromal cells under inflammatory conditions, especially through the action of the CB2 receptor. This research suggests that stimulating the endocannabinoid system may be an effective therapeutic option for inflammatory disorders.
PHARMACOLOGICAL REPORTS
(2021)
Article
Multidisciplinary Sciences
Brian J. Kwee, Johnny Lam, Adovi Akue, Mark A. KuKuruga, Kunyu Zhang, Luo Gu, Kyung E. Sung
Summary: This study evaluated the immunosuppressive capacity of IFN-gamma-licensed MSCs from multiple donors on different biomaterials, showing that specific integrin expression levels can impact the immunosuppressive capacity of MSCs.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Clinical Neurology
Amara Larpthaveesarp, Praneeti Pathipati, Samuel Ostrin, Anthony Rajah, Donna Ferriero, Fernando F. Gonzalez
Summary: The study found that both single-dose MSC/EPO and multidose systemic EPO can improve long-term neurobehavioral outcomes even when administered after a delay, with EPO showing the most overall effectiveness.
Article
Medicine, Research & Experimental
Tingting Qi, Xiaoming Xu, Yongzhen Guo, Yunlong Xia, Lu Peng, Congye Li, Fengyue Ding, Chao Gao, Miaomiao Fan, Min Yu, Huishou Zhao, Yuan He, Wenli Li, Chunxu Hai, Erhe Gao, Xing Zhang, Feng Gao, Yanhong Fan, Wenjun Yan, Ling Tao
Summary: In this study, it was discovered that overexpression of CSF2RB can enhance the cardiac homing ability of ADSCs and improve their protective effects against ischemic heart injury. CSF2RB overexpression also increases the migration capacity of ADSCs and inhibits their apoptosis under hypoxia/reoxygenation conditions. These findings suggest that CSF2RB overexpression can optimize the efficacy of intravenously delivered MSCs in the treatment of ischemic heart injury.
Article
Biochemistry & Molecular Biology
Dae-Won Lee, Sungho Shin, Jeong-Ho Kim, Cheolju Lee, In Yong Kim, Il-Hoan Oh
Summary: The inhibition of let-7 miRNAs enhances the therapeutic potential of MSCs by promoting cell proliferation, migration, and regenerative processes. It does not alter the fate of MSCs but instead induces functional changes accompanied by changes in proteomics. In addition, let-7-inhibited MSCs promote self-renewal of hematopoietic progenitor cells and enhance capillary formation in endothelial cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Anh T. L. Ngo, Hang M. Le, Nhung T. H. Trinh, Adriel Peng Guo Jun, Trung Q. Bach, Hue T. H. Bui, Van T. Hoang, Anh Bui, Liem T. Nguyen, Duc M. Hoang
Summary: The interaction between mesenchymal stem/stromal cells (MSCs) and preservation conditions plays a crucial role in maintaining cell viability and functionality. Different types of MSCs showed varying viability and metabolic profiles under different storage conditions. The cells released various growth factors, cytokines, and cytokines during preservation, maintaining their stemness and functionality.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Article
Agriculture, Dairy & Animal Science
Hannah J. Stage, Susanne Trappe, Katharina Soellig, Dagmar S. Trachsel, Katharina Kirsch, Cornelia Zieger, Roswitha Merle, Joerg R. Aschenbach, Heidrun Gehlen
Summary: This study investigated the proliferation and multilineage differentiation potential of equine adipose stem cells (ASCs) obtained from different tissue sources. The study found that ASCs had high proliferation and differentiation potential, but were unable to undergo cardiomyogenic differentiation. This study provides a basis for future research in equine medicine.
Review
Biochemistry & Molecular Biology
Beatriz Isabel Garcia-Martinez, Mirna Ruiz-Ramos, Jose Pedraza-Chaverri, Edelmiro Santiago-Osorio, Victor Manuel Mendoza-Nunez
Summary: Resveratrol has the potential to improve glucose and insulin levels in patients with type 2 diabetes, with significant improvement in glucose observed at doses of 500-1000 mg/day, and improvement in insulin seen at doses lower than 500 mg/day or greater than 1000 mg/day.
Article
Chemistry, Multidisciplinary
Sandra Salazar-Aguilar, Lucero del Mar Ruiz-Posadas, Jorge Cadena-Iniguez, Marcos Soto-Hernandez, Edelmiro Santiago-Osorio, Itzen Aguiniga-Sanchez, Victor M. Cisneros-Solano, Juan F. Aguirre-Medina, Alvaro D. Olivera
Summary: Microencapsulation based on chitosan was developed for the fruit extract of cv. Perla negra as an administration vehicle, and it proved to be a potential vehicle for its therapeutic administration without affecting the antiproliferative activity of the extract. Empty microspheres made with chitosan also showed an antiproliferative effect, and those loaded with extract showed cellular inhibition without affecting lymphocytes. Chitosan does not interfere with the biological activity of the metabolites incorporated into the microspheres.
APPLIED SCIENCES-BASEL
(2022)
Article
Biochemistry & Molecular Biology
Taide Laurita Arista-Ugalde, Edelmiro Santiago-Osorio, Alberto Monroy-Garcia, Juana Rosado-Perez, Itzen Aguiniga-Sanchez, Jorge Cadena-Iniguez, Graciela Gavia-Garcia, Victor Manuel Mendoza-Nunez
Summary: The consumption of Sechium edule powder has hypotensive, hypoglycemic, antioxidant, and anti-inflammatory effects in older adults with MetS, and it reduces the percentage of patients with MetS.
Review
Biochemistry & Molecular Biology
Beatriz Isabel Garcia-Martinez, Mirna Ruiz-Ramos, Jose Pedraza-Chaverri, Edelmiro Santiago-Osorio, Victor Manuel Mendoza-Nunez
Summary: The findings of the study indicate that resveratrol has a significant dose-response effect on glucose concentrations, HbA1c, and insulin levels. However, there is currently no established therapeutic dose.
Review
Immunology
Maria Victoria Legorreta-Haquet, Paola Santana-Sanchez, Luis Chavez-Sanchez, Adriana Karina Chavez-Rueda
Summary: The higher incidence of autoimmune diseases in females, especially during childbearing age, suggests that hormones like prolactin (PRL) play a significant role in determining the prevalence of autoimmunity in women. PRL acts as both a hormone and a cytokine, modulating immune responses and affecting disease activity in various autoimmune conditions, including systemic lupus erythematosus (SLE). It regulates cell proliferation, survival, and selection in the immune system, interferes with regulatory T cell activity, influences B cell tolerance, and promotes the production of autoantibodies and proinflammatory cytokines. PRL also affects dendritic cell function, neutrophil activity, and the behavior of innate and adaptive immune responses. This review highlights the importance of PRL and its actions in both innate and adaptive immune cells, contributing to a better understanding of SLE etiopathogenesis.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Ricardo Carreon-Talavera, Paola Santana-Sanchez, Ezequiel Moises Fuentes-Panana, Maria Victoria Legorreta-Haquet, Luis Chavez-Sanchez, Patricia Sofia Gorocica-Rosete, Adriana Karina Chavez-Rueda
Summary: PRL increases the concentration of self-reactive antibodies in the MRL/lpr SLE model by promoting the activation and proliferation of germinal center B cells and their differentiation into antibody-secreting cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Chemistry, Medicinal
Eugenia Elisa Delgado-Tiburcio, Jorge Cadena-Iniguez, Edelmiro Santiago-Osorio, Lucero del Mar Ruiz-Posadas, Israel Castillo-Juarez, Itzen Aguiniga-Sanchez, Marcos Soto-Hernandez
Summary: Cucurbitacins are secondary metabolites isolated from the Cucurbitaceae family, known for their analgesic, anti-inflammatory, antimicrobial, antiviral, and anticancer activities. This review summarizes recent pharmacokinetic studies on cucurbitacins, including their absorption, metabolism, distribution, and elimination processes, as well as the molecular mechanisms and anticancer capacity of these compounds.
Article
Chemistry, Analytical
Jorge Cadena-Iniguez, Itzen Aguiniga-Sanchez, Maria Teresa Uriostegui-Arias, Edelmiro Santiago-Osorio, Lucero del Mar Ruiz-Posadas, Marcos Soto-Hernandez
Summary: Sechium edule exhibits wide biological diversity and its hybrids and varieties have been used for food and traditional medicine. This research focuses on the antiproliferative activity of a specific genotype of Sechium edule var. amarus silvestrys on MCF7 breast cancer cells. The study found significant inhibitory activity in specific extracts, with certain active metabolites displaying even higher activity than the total extract.
Article
Nutrition & Dietetics
Ana Rocio Rivera-Martinez, Itzen Aguiniga-Sanchez, Jorge Cadena-Iniguez, Isabel Soto-Cruz, Alberto Monroy-Garcia, Guadalupe Gomez-Garcia, Edgar Ledesma-Martinez, Benny Weiss-Steider, Edelmiro Santiago-Osorio
Summary: The wild species Sechium chinantlense (SCH) has been found to have antitumor properties, inhibiting proliferation in cervical cancer cells without harming normal cells.
Article
Biochemistry & Molecular Biology
Monserrat Carrera-Martinez, Maria de L. Mora-Garcia, Rosario Garcia-Rocha, Benny Weiss-Steider, Juan J. Montesinos-Montesinos, Jorge Hernandez-Montes, Christian A. Don-Lopez, Alberto Monroy-Garcia
Summary: Recently, a potential link between CD73 and multidrug resistance protein (MRP) has been suggested in solid tumors. In this study, researchers investigated the involvement of CD73 and adenosine (ADO) signaling in MRP1 expression in cervical cancer (CC) cells. They found that ADO positively regulates MRP1 expression in a dose-dependent manner. Inhibiting CD73 expression and blocking ADO signaling through A2AR resulted in decreased MRP1 expression and enhanced sensitivity to cisplatin treatment in CC cells.
CELL BIOCHEMISTRY AND FUNCTION
(2023)
Article
Biochemistry & Molecular Biology
Beatriz Isabel Garcia-Martinez, Mirna Ruiz-Ramos, Jose Pedraza-Chaverri, Edelmiro Santiago-Osorio, Victor Manuel Mendoza-Nunez
Summary: Type 2 diabetes (T2D) is common among adults and affects their quality of life. Resveratrol (RV), a polyphenol with antioxidant and anti-inflammatory properties, has been studied in clinical trials for T2D but with conflicting results. In a randomized trial on older adults, 1000 mg/day of RV was found to have a more significant antioxidant effect compared to 500 mg/day, indicating its potential as an adjuvant therapy for T2D.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Victor Adrian Cortes-Morales, Luis Chavez-Sanchez, Leticia Rocha-Zavaleta, Sandra Espindola-Garibay, Alberto Monroy-Garcia, Marta Elena Castro-Manrreza, Guadalupe Rosario Fajardo-Orduna, Teresa Apresa-Garcia, Marcos Gutierrez-de la Barrera, Hector Mayani, Juan Jose Montesinos
Summary: This study found that MSCs derived from cervical cancer (CeCa-MSCs) have the ability to promote M2 macrophage polarization, unlike MSCs derived from normal cervix (NCx-MSCs). CeCa-MSCs increase the production of anti-inflammatory molecules (IL-10 and IL-1RA) to regulate antitumor immune responses.
Review
Biochemistry & Molecular Biology
David Hernandez-Alvarez, Juana Rosado-Perez, Graciela Gavia-Garcia, Taide Laurita Arista-Ugalde, Itzen Aguiniga-Sanchez, Edelmiro Santiago-Osorio, Victor Manuel Mendoza-Nunez
Summary: Human aging is a complex process involving biochemical and molecular changes driven by hallmarks of aging such as oxidative stress, chronic inflammation, and telomere shortening. Sarcopenia, a disease associated with hallmarks of aging, significantly affects functionality and quality of life. The relationship between telomere length, sarcopenia, and age-related mortality is not well-studied. Moderate physical exercise has been found to have positive effects on sarcopenia, reducing oxidative stress and inflammation, and protecting telomeric DNA. This review presents the current state of knowledge on the impact of physical exercise on telomeric maintenance and repair mechanisms in sarcopenia.
Review
Biochemistry & Molecular Biology
Guadalupe Rosario Fajardo-Orduna, Edgar Ledesma-Martinez, Itzen Aguiniga-Sanchez, Benny Weiss-Steider, Edelmiro Santiago-Osorio, Erik A. C. Wiemer, Ignacio Camacho-Arroyo, Jorge Melendez-Zajgla, Mahara A. Valverde Ramirez, Mauricio Rodriguez-Dorantes
Summary: Leukemias of the AML, CML, and CLL types are the most common blood cancers worldwide, posing a major global public health problem. Drug resistance, one of the main obstacles in leukemia treatment, may involve the epigenetic regulator SIRT1, which has been found to play a role in both promoting and inhibiting tumor development. This review explores the role of SIRT1 in drug-resistant leukemia and its potential as a therapeutic target.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)