Review
Biotechnology & Applied Microbiology
Chiyuan Ma, Xianglong Duan, Xiaohua Lei
Summary: Microgravity has profound effects on cell growth and differentiation, and three-dimensional (3D) culture scaffolds provide a more accurate representation of these effects compared to traditional two-dimensional (2D) culture methods. Hydrogels, in particular, mimic the in vivo environment and show promise in understanding the response of cells to microgravity. This review also highlights the importance of studying cell growth and differentiation under real or simulated microgravity conditions, and the potential of hydrogel-based 3D culture models in various applications.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Jessica Doernen, Thomas Dittmar
Summary: Regenerative medicine focuses on investigating therapeutic agents to promote regeneration after injury or in diseases, with MSCs and EVs being of interest for their roles in tissue regeneration. Clinical studies on MSCs have shown conflicting efficacies, and cell fusion may also play a role in regeneration alongside immune responses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Kengo Abe, Akihiro Yamashita, Miho Morioka, Nanao Horike, Yoshiaki Takei, Saeko Koyamatsu, Keisuke Okita, Shuichi Matsuda, Noriyuki Tsumaki
Summary: Allogeneic iPSC-derived cartilage organoids survive and integrate with surrounding native cartilage without immune reactions in a primate model of chondral defects in the knee joints, being remodeled and functioning as articular cartilage.
NATURE COMMUNICATIONS
(2023)
Review
Biotechnology & Applied Microbiology
Zeqing Zhao, Jin Liu, Michael D. Weir, Abraham Schneider, Tao Ma, Thomas W. Oates, Hockin H. K. Xu, Ke Zhang, Yuxing Bai
Summary: This review article presents the recent advances in PDLSC-based bone regeneration and periodontal tissue regeneration. By combining PDLSCs with various materials and scaffolds, as well as applying different osteoinductive agents and strategies, PDLSCs demonstrate the potential for bone and periodontal tissue regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Engineering, Biomedical
Woochan Kim, Yonghyun Gwon, Sunho Park, Hyoseong Kim, Jangho Kim
Summary: Three-dimensional stem cell culture systems have the potential to mimic complex interactions between cells and the extracellular matrix, and have unique properties that can be applied in therapeutics.
BIOACTIVE MATERIALS
(2023)
Review
Medicine, Research & Experimental
Mengyuan Wang, Ying Li, Hongqiao Wang, Meng Li, Xiaomin Wang, Rongzhen Liu, Daijun Zhang, Wenhua Xu
Summary: Corneal epithelial defects and excessive wound healing can cause severe complications. Stem cells offer a promising solution for regenerating the corneal epithelium and treating severe corneal epithelial injury. Biological scaffolds such as the amniotic membrane, fibrin, and hydrogels provide the necessary signals for stem cell proliferation and differentiation. Researchers have evaluated these scaffolds as potential therapeutic interventions for corneal disorders, identifying both benefits and drawbacks. This study provides a comprehensive overview of the history and use of stem cells in corneal repair, focusing on biological scaffolds used in stem cell transplantation and innovative materials under investigation.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Chemistry, Multidisciplinary
Na Yan, Bin Hu, Junchao Xu, Rong Cai, Zhenhai Liu, Dapeng Fu, Beibei Huo, Zhenhua Liu, Yuliang Zhao, Chunying Chen, Weihua Xu
Summary: Periodontitis poses a significant challenge in healthcare worldwide. Current treatment methods have limitations, but a novel stem cell-containing patch shows great potential in promoting periodontal regeneration and preventing epithelial invasion, offering a safe and effective strategy for repairing and protecting periodontal tissues.
Review
Developmental Biology
Jessica D. Manneken, Peter D. Currie
Summary: The cells of the innate immune system, particularly macrophages, play a vital role in tissue repair and stem cell activation. Recent studies have revealed the complex molecular processes involved in these mechanisms and the direct role of immune cells in coordinating these processes.
News Item
Multidisciplinary Sciences
Smriti Mallapaty
Summary: Research on using induced pluripotent stem cells for disease treatment has received substantial investment.
Review
Biochemistry & Molecular Biology
Yangwang Jin, Weixin Zhao, Ming Yang, Wenzhuo Fang, Guo Gao, Ying Wang, Qiang Fu
Summary: Urethral tissue engineering provides a new approach to repair urethral strictures, but the source, selection, and application conditions of cells need further investigation and improvement.
Review
Biochemistry & Molecular Biology
Batoul Chouaib, Mandana Haack-Sorensen, Franck Chaubron, Frederic Cuisinier, Pierre-Yves Collart-Dutilleul
Summary: Mesenchymal stem cell secretome or conditioned medium (MSC-CM) is a crucial starting point for various derived products and can be used for regenerative medicine. However, issues such as manufacturing protocols and quality control must be addressed before their clinical application. This review highlights the importance of standardized procedures in conditioned medium production and the influence of various factors on the quality of the secretome and its derivatives.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biotechnology & Applied Microbiology
Genglei Chu, Weidong Zhang, Feng Han, Kexin Li, Chengyuan Liu, Qiang Wei, Huan Wang, Yijie Liu, Fengxuan Han, Bin Li
Summary: Regenerative medicine for intervertebral disc (IVD) disease, using chondrocytes, IVD cells, and stem cells, has reached clinical trials for back pain treatment. The survival and function of mesenchymal stem cells (MSCs) depend on the microenvironment in which they are transplanted, which is influenced by the complex microenvironment in the degenerative disc. Understanding how MSCs and endogenous disc cells survive and thrive in the harsh microenvironment is important. The use of materials containing stem cells and their natural microenvironment has shown good clinical effects.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
David A. Hart, Norimasa Nakamura
Summary: After injuries to musculoskeletal tissues, an inflammatory response occurs to promote endogenous repair, but it often results in functionally inferior scar tissue. Injuries in adults no longer have the anabolic processes that contribute to growth and maturation, which can lead to degenerative conditions such as osteoarthritis. Researchers have been exploring cellular therapies, such as Platelet-rich Plasma and mesenchymal stem/medicinal signaling cells, to enhance tissue repair and regeneration. However, implanting these cells or engineered constructs into an inflammatory adult environment may compromise their potential for regeneration. Therefore, it may be necessary to neutralize the inflammatory environment and create an anabolic environment for optimal outcomes.
Review
Cell Biology
Ahmed Nugud, Latifa Alghfeli, Moustafa Elmasry, Ibrahim El-Serafi, Ahmed T. El-Serafi
Summary: Biomaterials and tissue regeneration are two active fields of research that can be effectively combined to enhance stem cell expansion and differentiation for tissue regeneration. However, further analysis and investigation are needed to achieve significant advancements in this field.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell & Tissue Engineering
Xiaotong Zhao, Qiong Li, Zhikun Guo, Zongjin Li
Summary: Stem cell therapy is recognized as a promising strategy for treating degenerative diseases, but low cell retention and survival rates limit its clinical applications. Emerging biomaterials that provide a supportive cellular microenvironment have optimized the therapeutic efficacy of stem cells in injured tissues. Advances in engineered microenvironment, through co-transplanting with synthetic and tissue-derived biomaterials, offer unprecedented opportunities for significant influences in tissue repair and regeneration.
STEM CELL RESEARCH & THERAPY
(2021)
Editorial Material
Cell & Tissue Engineering
Elizabeth A. Blaber, Graham C. Parker
STEM CELLS AND DEVELOPMENT
(2018)
Article
Cell & Tissue Engineering
Jessica Cardenas Turner, George Collins, Elizabeth A. Blaber, Eduardo A. C. Almeida, Treena L. Arinzeh
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2020)
Article
Cell Biology
Sherina Malkani, Christopher R. Chin, Egle Cekanaviciute, Marie Mortreux, Hazeem Okinula, Marcel Tarbier, Ann-Sofie Schreurs, Yasaman Shirazi-Fard, Candice G. T. Tahimic, Deyra N. Rodriguez, Brittany S. Sexton, Daniel Butler, Akanksha Verma, Daniela Bezdan, Ceyda Durmaz, Matthew MacKay, Ari Melnick, Cem Meydan, Sheng Li, Francine Garrett-Bakelman, Bastian Fromm, Ebrahim Afshinnekoo, Brad W. Langhorst, Eileen T. Dimalanta, Margareth Cheng-Campbell, Elizabeth Blaber, Jonathan C. Schisler, Charles Vanderburg, Marc R. Friedlaender, J. Tyson McDonald, Sylvain Costes, Seward Rutkove, Peter Grabham, Christopher E. Mason, Afshin Beheshti
Article
Multidisciplinary Sciences
Yen-Ruh Wuu, Burong Hu, Hazeem Okunola, Amber M. Paul, Elizabeth A. Blaber, Margareth Cheng-Campbell, Afshin Beheshti, Peter Grabham
Article
Multidisciplinary Sciences
Amber M. Paul, Margareth Cheng-Campbell, Elizabeth A. Blaber, Sulekha Anand, Sharmila Bhattacharya, Sara R. Zwart, Brian E. Crucian, Scott M. Smith, Robert Meller, Peter Grabham, Afshin Beheshti
Article
Multidisciplinary Sciences
Eliah G. Overbey, Amanda M. Saravia-Butler, Zhe Zhang, Komal S. Rathi, Homer Fogle, Willian A. da Silveira, Richard J. Barker, Joseph J. Bass, Afshin Beheshti, Daniel C. Berrios, Elizabeth A. Blaber, Egle Cekanaviciute, Helio A. Costa, Laurence B. Davin, Kathleen M. Fisch, Samrawit G. Gebre, Matthew Geniza, Rachel Gilbert, Simon Gilroy, Gary Hardiman, Raul Herranz, Yared H. Kidane, Colin P. S. Kruse, Michael D. Lee, Ted Liefeld, Norman G. Lewis, J. Tyson McDonald, Robert Meller, Tejaswini Mishra, Imara Y. Perera, Shayoni Ray, Sigrid S. Reinsch, Sara Brin Rosenthal, Michael Strong, Nathaniel J. Szewczyk, Candice G. T. Tahimic, Deanne M. Taylor, Joshua P. Vandenbrink, Alicia Villacampa, Silvio Weging, Chris Wolverton, Sarah E. Wyatt, Luis Zea, Sylvain Costes, Jonathan M. Galazka
Summary: With the advancement of transcriptomic technologies, NASA GeneLab and associated working groups have developed a consensus pipeline for analyzing short-read RNA-sequencing data from spaceflight experiments, which is now publicly available. This aims to promote transparency, reproducibility, and reusability of data, provide a template for future data processing, and encourage cross-analysis of data from different databases.
Article
Cell & Tissue Engineering
Cassandra M. Juran, Justina Zvirblyte, Margareth Cheng-Campbell, Elizabeth A. Blaber, Eduardo A. C. Almeida
Summary: In the experiment, cyclically stretched Cdkn1a(-/-) cells showed nearly 4 times more proliferation compared to wildtype, while static Cdkn1a(-/-) cells exhibited a 2.5-fold increase. Additionally, single cell RNAseq analyses revealed that expression of Cdkn1a was strongly suppressed by cyclic stretch in early and late osteoblasts, with minimal effect on the progenitor population. Furthermore, both mechanical stretch and Cdkn1a deletion led to a shift in cell population from osteoprogenitors to osteoblasts, indicating enhanced differentiation.
STEM CELL RESEARCH
(2021)
Article
Multidisciplinary Sciences
Meghana Pendyala, Paige S. Woods, Douglas K. Brubaker, Elizabeth A. Blaber, Tannin A. Schmidt, Deva D. Chan
Summary: The study demonstrates that cyclic loading can modulate the endogenous production of hyaluronan, PRG4, and cytokines from synovial fibroblasts. The concentration of hyaluronan and PRG4 is affected by the magnitude of strain, and different strain levels can result in distinct cytokine profiles.
Meeting Abstract
Ophthalmology
Tasneem Putliwala Sharma, Ryan C. S. Miller, Shahna P. ShahulHameed, Angela Kubik, Noah Allen, Selin Altinok, Leah E. Oswalt, Rebekah Sanchez-Hodge, Jonathan Cummings Schisler, Elizabeth Blaber, Afshin Beheshti
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Meeting Abstract
Cell & Tissue Engineering
Alexander Regner, Kalin Gibbons, Derek Nesbitt, Elizabeth Blaber, Trevor Lujan, Claire Fitzpatrick, Mary C. Farach-Carson, Danielle Wu, Gunes Uzer
TISSUE ENGINEERING PART A
(2022)
Meeting Abstract
Endocrinology & Metabolism
Meghana Pendyala, Samuel Stephen, Elizabeth Blaber, Deepak Vashishth, Deva Chan
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)
Meeting Abstract
Endocrinology & Metabolism
Meghana Pendyala, Samuel Stephen, Elizabeth Blaber, Deepak Vashishth, Deva Chan
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)
Meeting Abstract
Immunology
Amber M. Paul, Margareth Cheng-Campbell, Elizabeth A. Blaber, Sulekha Anand, Sharmila Bhattacharya, Sara R. Zwart, Brian E. Crucian, Scott M. Smith, Robert Meller, Peter Grabham, Afshin Beheshti
JOURNAL OF IMMUNOLOGY
(2021)
Article
Oncology
Jeffrey S. Willey, Richard A. Britten, Elizabeth Blaber, Candice G. T. Tahimic, Jeffrey Chancellor, Marie Mortreux, Larry D. Sanford, Angela J. Kubik, Michael D. Delp, Xiao Wen Mao
Summary: Both microgravity and radiation exposure in the spaceflight environment have been identified as hazards to astronaut health and performance. Study on the biology and risks associated with these hazards have mainly focused on individual aspects, but combined hazard studies are essential for understanding the risks astronauts face outside of low earth orbit. This review highlights the combined effects of radiation and reduced weight-bearing from ground-based analog models for microgravity and radiation.
JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART C-TOXICOLOGY AND CARCINOGENESIS
(2021)
Meeting Abstract
Endocrinology & Metabolism
Elizabeth Blaber, Ann-Sofie Schreurs
JOURNAL OF BONE AND MINERAL RESEARCH
(2018)