Article
Biochemistry & Molecular Biology
Manuel Mata, Santiago Peydro, Jose Javier Martin de Llano, Maria Sancho-Tello, Carmen Carda
Summary: Periodontitis is an inflammatory disease that can lead to tooth loss. This study examined the role of surface microtopography in the differentiation of human dental pulp stem cells into cementoid-like secreting cells. The results showed that the cells cultured on dentin surfaces exhibited an odontoblastic phenotype, while cells cultured on cementum surfaces generated a well-organized tissue consisting of secretory cells and dense fibrous connective tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Dentistry, Oral Surgery & Medicine
Chao Wang, Yuejun Wang, Haiyan Wang, Haoqing Yang, Yangyang Cao, DengSheng Xia, Yu Cao, Zhipeng Fan
Summary: The study found that over-expression of SFRP2 significantly enhanced dentinogenesis in DPSCs, promoting dentin regeneration, which may have potential clinical applications.
Article
Multidisciplinary Sciences
Muhammad Chair Effendi, Ahmad Taufiq, Boy Muchlis Bachtiar, Endang Winiati Bachtiar, Ellyza Herda
Summary: The study revealed that DPSCs exposed to NMT showed enhanced proliferation and differentiation, demonstrating dentin formation and extracellular matrix formation.
Article
Dentistry, Oral Surgery & Medicine
Vellore Kannan Gopinath, Sheela Soumya, V. Yogeshwar Chakrapani, T. S. Sampath Kumar
Summary: This study developed electrospun nanofibrous membranes blending PCL with HA, with PCL10%HA membrane showing the most favorable effects on cell attachment, upregulation of gene expression, and Ca/P ratio compared to PCL and PCL15%HA membranes in inducing differentiation of IDPSCs into odontoblasts.
DENTAL MATERIALS JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Cristina- Bucchi, Ella Ohlsson, Josep Maria de Anta, Melanie Woelflick, Kerstin Galler, Maria Cristina Manzanares-Cespedes, Matthias Widbiller
Summary: Pluripotent stem cells derived from the amnion show the ability to adhere to dentin and mineralize in vitro, but exhibit unfavorable proliferation behavior and fail to undergo odontoblastic transition.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Kunihiro Kajiura, Naoki Umemura, Emika Ohkoshi, Takahisa Ohta, Nobuo Kondoh, Satoshi Kawano
Summary: The study demonstrates that shikonin can induce odontoblastic differentiation of dental pulp stem cells through the involvement of the AKT-mTOR signaling pathway and CD44. While CD44 expression is crucial for odontoblastic differentiation of dental pulp stem cells, the relationship between shikonin-induced expression of DSPP and the AKT-mTOR signaling pathway and CD44 expression remains to be fully elucidated.
CONNECTIVE TISSUE RESEARCH
(2021)
Article
Cell & Tissue Engineering
Xin He, Zhan Yang, Xiao-Yang Chu, Yun-Xia Li, Biao Zhu, Yan-Xia Huang, Wei Wang, Chun-Yan Gao, Xu Chen, Chun-Yan Zheng, Kai Yang, Dong-Liang Zhang
Summary: The present study revealed the vital role of the ROR2/MSX2/NSUN2 axis in the regulation of DPSC senescence, thereby providing a potential target for antagonizing DPSC aging.
Article
Anatomy & Morphology
Giovanna Sarra, Manoel Eduardo de Lima Machado, Hector Valentin Caballero-Flores, Maria Stella Moreira, Ana Clara Fagundes Pedroni, Marcia Martins Marques
Summary: This study evaluated the effects of human dental pulp stem cell conditioned medium on dental pulp tissue response to vital pulp therapy. The results showed that the conditioned medium, when combined with MTA, promoted dentin-pulp complex regeneration, indicating promising potential for regenerative dentistry studies.
Article
Cell & Tissue Engineering
Jie Chen, Huaxing Xu, Kun Xia, Shuhua Cheng, Qi Zhang
Summary: In this study, it was found that RvE1 promotes pulp inflammation resolution and dentin regeneration, and positively influences the proliferation, chemotaxis, and differentiation of LPS-stimulated hDPSCs. The effects of RvE1 on DPSCs were mediated at least partially through AKT, ERK, and rS6-associated signaling in the inflammatory microenvironment. RvE1 shows promising application potential in regenerative endodontics.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell & Tissue Engineering
Mayu Matsumura-Kawashima, Kenichi Ogata, Masafumi Moriyama, Yuka Murakami, Tatsuya Kawado, Seiji Nakamura
Summary: DPSC-CM contained more secreted factors with tissue-regenerating mechanisms, such as cell proliferation, anti-inflammatory effects, and immunomodulatory effects. DPSC-CM was more effective in suppressing the activated T cells than other groups. The stimulated salivary flow rate increased in SS mice with DPSC-CM compared with that in the other groups.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Lina Yu, Liting Zeng, Zeyu Zhang, Guanxiong Zhu, Zidan Xu, Junyi Xia, Jinlong Weng, Jiang Li, Janak Lal Pathak
Summary: This study found that cannabidiol enhances the proliferation, migration, and osteogenic/odontogenic differentiation of dental pulp stem cells (DPSCs). CBD also exhibits anti-inflammatory, chemotactic, anti-microbial, and tissue regenerative potentials. These findings suggest the potential application of CBD in dental pulp and bone regeneration based on DPSCs.
Article
Multidisciplinary Sciences
Leslie S. Babonis, Camille Enjolras, Joseph F. Ryan, Mark Q. Martindale
Summary: This study reveals the developmental mechanism of a truly novel cell type, cnidocytes. Cnidocytes develop by suppressing the neural fate of a subset of neurons expressing RFamide. A single regulatory gene, a C2H2-type zinc finger transcription factor (ZNF845), plays a crucial role in the development of cnidocytes and emerged through domain shuffling in the ancestor of cnidarians.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Engineering, Biomedical
Ju Won Jung, So Young Park, Eun Jin Seo, Il Ho Jang, Yeji Park, Dasun Lee, Dohyun Kim, Jin Man Kim
Summary: Dental pulp-derived stromal cells (DPSCs) are important for maintaining the tissue integrity of the pulp-dentin complex. In this study, we demonstrate the biological functions and significance of the oxytocin receptor (OXTR) in DPSCs. Blocking OXTR activity promotes DPSC differentiation and is involved in extracellular matrix (ECM) remodeling. The genetic changes mediated by OXTR are regulated by the transcriptional activity of Yes-associated protein (YAP). These findings provide new insights into the regenerative process of the pulp-dentin complex.
Article
Dentistry, Oral Surgery & Medicine
Hengameh Bakhtiar, Mohammad Reza Mousavi, Sarah Rajabi, Mohammad Pezeshki-Modaress, Alireza Ayati, Azin Ashoori, Mohammad Reza Ellini, Khadijeh Baaji, Amir Kamali, Zhila Abediasl, Amir Azarpazhooh, Anil Kishen
Summary: This study aimed to fabricate and characterize a novel injectable human amniotic membrane (HAM) hydrogel scaffold crosslinked with genipin, enabling the proliferation of Dental Pulp Stem Cells (DPSCs) and optimizing pulp regeneration. Genipin crosslinking improved the biodegradability of injectable HAM hydrogels and conferred higher biocompatibility. Hydrogels encapsulated with DPSCs can support stem cell viability and proliferation, and highly vascularized pulp-like tissue formation by this biomaterial displayed potential for pulp regeneration.
Article
Medicine, General & Internal
Aishwarya Balasankar, Shu-Yi Claire Chan, Venkata Pakala Sudheer Babu, Gary Yam, Goh Bee Tin, Shweta Singhal
Summary: Dental pulp stem cells (DPSCs) derived from the neural crest are easily accessible MSCs that have potential for retinal therapy. This study shows that DPSCs maintain their mesenchymal phenotype and upregulate retinal markers under differentiation conditions. However, current markers are inadequate for isolating neural crest cells in DPSCs, highlighting the need for more specific markers.
FRONTIERS IN MEDICINE
(2022)
Article
Andrology
Cathy A. Goldstein, Louise M. O'Brien, Ingrid L. Bergin, Thomas L. Saunders
SYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE
(2018)
Article
Genetics & Heredity
Jung-Wook Kim, Hong Zhang, Figen Seymen, Mine Koruyucu, Yuanyuan Hu, Jenny Kang, Youn J. Kim, Atsushi Ikeda, Yelda Kasimoglu, Merve Bayram, Chuhua Zhang, Kazuhiko Kawasaki, John D. Bartlett, Thomas L. Saunders, James P. Simmer, Jan C-C. Hu
Article
Biochemistry & Molecular Biology
Christopher M. Allan, Patrick J. Heizer, Yiping Tu, Norma P. Sandoval, Rachel S. Jung, Jazmin E. Morales, Eniko Sajti, Ty D. Troutman, Thomas L. Saunders, Darren A. Cusanovich, Anne P. Beigneux, Casey E. Romanoski, Loren G. Fong, Stephen G. Young
JOURNAL OF LIPID RESEARCH
(2019)
Article
Dentistry, Oral Surgery & Medicine
R. Said, L. Zheng, T. Saunders, M. Zeidler, S. Papagerakis, P. Papagerakis
JOURNAL OF DENTAL RESEARCH
(2019)
Article
Multidisciplinary Sciences
Brian T. Emmer, Paul J. Lascuna, Vi T. Tang, Emilee N. Kotnik, Thomas L. Saunders, Rami Khoriaty, David Ginsburg
Article
Multidisciplinary Sciences
Xiaohua Zheng, Edward B. Arias, Nathan R. Qi, Thomas L. Saunders, Gregory D. Cartee
Review
Genetics & Heredity
Thomas M. Lanigan, Huira C. Kopera, Thomas L. Saunders
Article
Multidisciplinary Sciences
Dandrich Lim, Ko-Chien Wu, Arthur Lee, Thomas L. Saunders, Helena H. Ritchie
Summary: Dentin Sialoprotein (DSP) and phosphophoryn (PP) are two major non-collagenous proteins in dentin that play essential roles in dentin formation. Using transgenic technology, the DSPP gene knockout defect in mice can be partially or completely rescued, indicating the importance of DSPP in tooth development.
Article
Multidisciplinary Sciences
Richard King, Zesen Lin, Ginette Balbin-Cuesta, Gregg Myers, Ann Friedman, Guojing Zhu, Beth McGee, Thomas L. Saunders, Ryo Kurita, Yukio Nakamura, James Douglas Engel, Pavan Reddy, Rami Khoriaty
Summary: The expression of full SEC23A protein from endogenous regulatory elements of Sec23b can completely rescue the SEC23B-deficient mouse phenotype, while erythroid-specific deletion of all four Sec23 alleles in mice results in mid-embryogenesis death with features of CDAII. The study also suggests a novel therapeutic strategy for CDAII by increasing the expression of SEC23A in human erythroid cells.
Article
Medicine, Research & Experimental
Di Chen, Elizabeth D. Hughes, Thomas L. Saunders, Jiangping Wu, Magda N. Hernandez Vasquez, Taija Makinen, Philip D. King
Summary: This study demonstrates that RASA1 and EPHB4 function in the same signaling pathway to protect against the development of CM-AVM, independent of physical interaction. These findings have important implications for possible treatment of this disease.
Article
Multidisciplinary Sciences
Yuki Matsushita, Jialin Liu, Angel Ka Yan Chu, Chiaki Tsutsumi-Arai, Mizuki Nagata, Yuki Arai, Wanida Ono, Kouhei Yamamoto, Thomas L. Saunders, Joshua D. Welch, Noriaki Ono
Summary: This study discovered a new class of skeletal stem cells in the bone marrow's endosteal space, which are abundant in young bone marrow, express Fgfr3, and have osteoblastic-chondrocytic transitional identities. These cells can transform into cells that generate bone tumors.
NATURE COMMUNICATIONS
(2023)
Article
Medicine, Research & Experimental
Yang Zhao, Guizhen Zhao, Ziyi Chang, Tianqing Zhu, Ying Zhao, Haocheng Lu, Chao Xue, Thomas L. Saunders, Yanhong Guo, Lin Chang, Y. Eugene Chen, Jifeng Zhang
Summary: To achieve specific and efficient smooth muscle cell (SMC) targeted gene deletion, we used CRISPR/Cas9-mediated homologous recombination to generate Cre-knockin mice by inserting CreNLSP2A or CreERT2-P2A sequences into the homologous arm surrounding the translation start site of the Myh11 gene. We evaluated the efficiency, specificity, tamoxifen-dependent controllability, and functionality of Cre-mediated recombination in both sexes using reporter mice. These models will expand the research toolbox and facilitate unbiased and comprehensive research in SMCs and SMC-dependent cardiovascular diseases.
Article
Neurosciences
Jeffrey R. Pettibone, Jai Y. Yu, Rifka C. Derman, Thomas W. Faust, Elizabeth D. Hughes, Wanda E. Filipiak, Thomas L. Saunders, Carrie R. Ferrario, Joshua D. Berke
Article
Hematology
Lingjun Zhang, Yang Dai, Ping Huang, Thomas L. Saunders, David A. Fox, Jijun Xu, Feng Lin
Article
Pathology
Lisa C. Flores, Madeline G. Roman, Geneva M. Cunningham, Christie Cheng, Sara Dube, Colton Allen, Holly Van Remmen, Gene B. Hubbard, Thomas L. Saunders, Yuji Ikeno
PATHOBIOLOGY OF AGING AND AGE-RELATED DISEASES
(2018)