Article
Cell Biology
Furkan Yigitbilek, Sabena M. Conley, Hui Tang, Ishran M. Saadiq, Kyra L. Jordan, Lilach O. Lerman, Timucin Taner
Summary: MSCs from liver and adipose tissue showed similar proliferation and migration properties, but had differences in immunomodulatory and pro-angiogenic abilities. Selecting cell-based therapy according to the specific characteristics of different cell sources may be important for treating certain diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell & Tissue Engineering
Shu Hui Neo, Zhisheng Her, Rashidah Othman, Ching Ann Tee, Li Ching Ong, Yuehua Wang, Irwin Tan, Jaylen Tan, Yanmeng Yang, Zheng Yang, Qingfeng Chen, Laurie A. Boyer
Summary: Culturing MSCs at high confluency enhances their immunomodulatory functions, inhibiting T cell proliferation and pro-inflammatory cytokine secretion, leading to improved therapeutic efficacy in GVHD mouse model.
STEM CELL RESEARCH & THERAPY
(2023)
Review
Immunology
Ayman F. Refaie, Batoul L. Elbassiouny, Malgorzata Kloc, Omaima M. Sabek, Sherry M. Khater, Amani M. Ismail, Rania H. Mohamed, Mohamed A. Ghoneim
Summary: MSC-based therapy for T1DM shows promise, but there are conflicting views on their immunological profile. Research focuses on understanding the mechanisms of their immunomodulatory functions and exploring ways to enhance their effects.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Immunology
Likun Zheng, Lei Zhang, Yixuan Guo, Xintong Xu, Zhaoyun Liu, Zhenyu Yan, Rong Fu
Summary: Myelodysplastic syndrome (MDS) is a common hematological malignant disease characterized by abnormal proliferation of malignant hematopoietic stem cells in the bone marrow. The dysfunction of mesenchymal stromal cells (MSCs) in the bone marrow microenvironment plays a critical role in MDS progression through modulation of immune cell function, enabling evasion of immune surveillance by MDS-associated malignant cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Endocrinology & Metabolism
Qiong Wu, Shuangli He, Yu Zhu, Shiming Pu, Zuping Zhou
Summary: This study demonstrated the effectiveness of adipose-derived stromal/stem cells (ASCs) in treating obesity and aging in aged mice. ASCs treatment led to significant weight loss, improved organ function, increased antioxidant capacity, enhanced lipid metabolism, and reduced inflammation in old mice. The positive effects of ASCs suggest their potential as primary antiaging and antiobesity agents for the elderly.
Review
Biochemistry & Molecular Biology
Raphael Gorodetsky, Wilhelm K. Aicher
Summary: MSCs from different sources can release pro-regenerative and anti-inflammatory factors, with hPSCs from the fresh human placenta being a preferred source for abundant cell harvesting. The f-hPSCs from neonate tissues of the placenta can undergo extended expansion with low risk of senescence, making them competent cells for therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Materials Science, Multidisciplinary
Ilaria Abdel Aziz, Leonardo Maver, Chiara Giannasi, Stefania Niada, Anna T. Brini, Maria Rosa Antognazza
Summary: Recent progress in regenerative medicine and tissue engineering has been driven by the investigation of crucial cues and the development of functional materials. Optical stimulation has emerged as an effective strategy for directing stem cell differentiation and modulating cell physiology. However, the effects of optical excitation on undifferentiated cells have been poorly studied. This work demonstrates the modulation of cell membrane potential and intracellular calcium signaling in undifferentiated stem cells upon optical excitation.
JOURNAL OF MATERIALS CHEMISTRY C
(2022)
Article
Cell & Tissue Engineering
Rose Triantafillia Psaroudis, Urvashi Singh, Maximilien Lora, Peter Jeon, Abigail Boursiquot, Ursula Stochaj, David Langlais, Ines Colmegna
Summary: This study identifies DPP4 gene expression and CD26 protein abundance as markers of replicative senescence in MSC(AT). MSCs with high CD26 expression have reduced immunopotency, and senolytic treatment can decrease the frequency of CD26(high) MSCs.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Agnieszka Krawczenko, Aleksandra Klimczak
Summary: MSCs, known for their regenerative and immunomodulatory properties, are typically isolated from bone marrow and adipose tissue. This review focuses on MSCs derived from adipose tissue and their secretome in regeneration processes, with a special emphasis on their contribution to angiogenic processes. Various clinical trials have been registered to explore the application of AT-MSCs and their secretome in treating different medical conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Adrienne Wright, Orman (Larry) Snyder, Hong He, Lane K. Christenson, Sherry Fleming, Mark L. Weiss
Summary: Many cell types, including cancer cells, release extracellular vesicles (EVs) that expose tissue factor (TF). It is unclear whether MSC-EVs have a risk of thromboembolism due to TF expression. In this study, the researchers investigated the expression of TF and the procoagulant activity of MSC-EVs. They also examined the impact of different EV isolation methods and cell culture expansion on EV yield, characterization, and potential risk. The results showed that MSC-EVs express TF and have procoagulant activity. Therefore, when using MSC-derived EVs as a therapeutic agent, considerations should be given to TF expression, procoagulant activity, and thromboembolism risk.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Surgery
Sarah C. Stokes, Sandra K. Kabagambe, Chelsey J. Lee, Aijun Wang, Diana L. Farmer, Priyadarsini Kumar
Summary: This study demonstrates that placental mesenchymal stromal cells (PMSCs) from different gestational ages have varying levels of neuroprotective function. Some cell lines showed enhanced neuroprotective capabilities, expanding the donor pool of PMSCs for clinical use. Further studies are needed to explore potential differences in cell function at different gestational ages.
JOURNAL OF SURGICAL RESEARCH
(2022)
Article
Cell Biology
Maria Serena Piccinno, Tiziana Petrachi, Marco Pignatti, Alba Murgia, Giulia Grisendi, Olivia Candini, Elisa Resca, Valentina Bergamini, Francesco Ganzerli, Alberto Portone, Ilenia Mastrolia, Chiara Chiavelli, Ilaria Castelli, Daniela Bernabei, Mara Tagliazucchi, Elisa Bonetti, Francesca Lolli, Giorgio De Santis, Massimo Dominici, Elena Veronesi
Summary: This study investigates the use of adipose-derived mesenchymal stromal/stem cells (AD-MSCs) in autologous fat transfer (AFT) for tissue reconstruction. The results show that AD-MSCs can improve graft persistence and reduce tissue resorption after AFT. The implanted AD-MSCs can persist and differentiate into mature adipocytes, leading to better outcomes in terms of graft weight and reduced cyst formation. This suggests a long-term protective and anti-inflammatory/proangiogenic role of AD-MSCs in autologous AFT tissue resorption.
Article
Biochemistry & Molecular Biology
Aniko Kun-Varga, Barbara Guban, Vanda Miklos, Shahram Parvaneh, Melinda Guba, Diana Szucs, Tamas Monostori, Janos Varga, Akos Varga, Zsolt Razga, Zsuzsanna Bata-Csorgo, Lajos Kemeny, Klara Megyeri, Zoltan Vereb
Summary: The proper functioning of mesenchymal stem cells (MSCs) is crucial for the body's homeostasis. Inflammation and infection can alter the function of MSCs, which in turn affects tissue regeneration and immunological status. This study aimed to investigate the response of adipose-derived MSCs (ADMSCs) to HSV infection in vitro.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Dan Xing, Kai Wang, Jun Wu, Yu Zhao, Wei Liu, Jiao Jiao Li, Tingting Gao, Deng Yan, Liu Wang, Jie Hao, Jianhao Lin
Summary: The study demonstrates that multiple injections of ES-MSCs can improve osteoarthritis in a rat model. The repeated doses group showed more proteoglycan and less cartilage loss, along with lower Mankin scores, indicating the potential of ES-MSCs as a regenerative therapy for OA.
Review
Cell Biology
Yuan Liu, Christina Holmes
Summary: This review explores the tissue regeneration potential of EVs isolated from bone marrow and adipose tissue, the two most common clinical sources of adult MSCs, focusing on their angiogenic, osteogenic, and immunomodulatory potentials. Other important issues in the development of MSC-derived EV based therapies are also discussed.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Immunology
Carla Cendon, Weijie Du, Pawel Durek, Yuk-Chien Liu, Tobias Alexander, Lindsay Serene, Xinyi Yang, Gilles Gasparoni, Abdulrahman Salhab, Karl Nordstroem, Tina Lai, Axel R. Schulz, Anna Rao, Gitta A. Heinz, Ana L. Stefanski, Anne Claussnitzer, Katherina Siewert, Thomas Doerner, Hyun-Dong Chang, Hans-Dieter Volk, Chiara Romagnani, Zhihai Qin, Sebastian Hardt, Carsten Perka, Simon Reinke, Jorn Walter, Mir-Farzin Mashreghi, Kevin Thurley, Andreas Radbruch, Jun Dong
Summary: CD4(+) T-RM cells are mobilized into the blood within 16 to 48 h after immunization with the measles-mumps-rubella (MMR) vaccine, and they make significant contribution to the systemic immune reaction.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Respiratory System
Andrej Mantei, Tim Meyer, Mariana Schuermann, Christiane Bessler, Harald Bias, David Krieger, Torsten Bauer, Petra Bacher, Johannes Helmuth, Hans-Dieter Volk, Dirk Schuermann, Alexander Scheffold, Christian Meisel
Summary: This study established a standardized TB-Flow assay that can rapidly and reliably discriminate TB infection from TB disease based on multiparameter analysis of TB antigen-reactive CD4(+) T-cells.
EUROPEAN RESPIRATORY JOURNAL
(2022)
Article
Biotechnology & Applied Microbiology
Sybille Landwehr-Kenzel, Leonie Mueller-Jensen, Joern-Sven Kuehl, Mohamed Abou-el-Enein, Henrike Hoffmann, Sandra Muench, Daniel Kaiser, Andy Roemhild, Horst von Bernuth, Mirjam Voeller, Michael Schmueck-Henneresse, Bernd Gruhn, Ulrik Stervbo, Nina Babel, Hans-Dieter Volk, Petra Reinke
Summary: This study explored the use of adoptive transfer of ex vivo expanded regulatory T cells (Tregs) for the treatment of chronic graft-versus-host disease (GvHD). The results showed that Tregs therapy was well tolerated and capable of modulating established adverse T cell mediated allo-response in patients.
Article
Immunology
Sophie Steiner, Tatjana Schwarz, Victor M. Corman, Laura Gebert, Malte C. Kleinschmidt, Alexandra Wald, Sven Glaeser, Jan M. Kruse, Daniel Zickler, Alexander Peric, Christian Meisel, Tim Meyer, Olga L. Staudacher, Kirsten Wittke, Claudia Kedor, Sandra Bauer, Nabeel Al Besher, Ulrich Kalus, Axel Pruss, Christian Drosten, Hans-Dieter Volk, Carmen Scheibenbogen, Leif G. Hanitsch
Summary: This study reports clinical and immunological findings in PAD patients with severe/fatal COVID-19 and undetectable humoral immune response to SARS-CoV-2. Robust T cell response was observed in all examined PAD patients, indicating the importance of T cell immunity in COVID-19.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Julia Sbierski-Kind, Sophia Grenkowitz, Stephan Schlickeiser, Arvid Sandforth, Marie Friedrich, Desiree Kunkel, Rainer Glauben, Sebastian Brachs, Knut Mai, Andrea Thuermer, Aleksandar Radonic, Oliver Drechsel, Peter J. Turnbaugh, Jordan E. Bisanz, Hans-Dieter Volk, Joachim Spranger, Reiner Jumpertz von Schwartzenberg
Summary: Caloric restriction can improve metabolic health, reduce body fat accumulation, and enhance glucose tolerance, while delaying immune senescence by reducing specific cellular immune responses. This suggests that modulation of the gut microbiome could lead to the development of new therapeutic options for metabolic diseases.
Article
Medicine, Research & Experimental
Lena Peter, Desiree Jacqueline Wendering, Stephan Schlickeiser, Henrike Hoffmann, Rebecca Noster, Dimitrios Laurin Wagner, Ghazaleh Zarrinrad, Sandra Muench, Samira Picht, Sarah Schulenberg, Hanieh Moradian, Mir-Farzin Mashreghi, Oliver Klein, Manfred Gossen, Toralf Roch, Nina Babel, Petra Reinke, Hans-Dieter Volk, Leila Amini, Michael Schmueck-Henneresse
Summary: Solid organ transplant (SOT) recipients with compromised immune response are at high risk of severe COVID-19. This research proposes the adoptive transfer of tacrolimus-resistant SARS-CoV-2-specific T cells to enhance immune response in immunosuppressed patients. Promising results from laboratory analysis support the potential clinical validation of this approach in transplantation. The combinatory use of tacrolimus and tacrolimus-resistant T cells is suggested to prevent immunopathology and facilitate viral clearance in severe COVID-19 cases.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2022)
Article
Medicine, Research & Experimental
Jonas Kath, Weijie Du, Alina Pruene, Tobias Braun, Bernice Thommandru, Rolf Turk, Morgan L. Sturgeon, Gavin L. Kurgan, Leila Amini, Maik Stein, Tatiana Zittel, Stefania Martini, Lennard Ostendorf, Andreas Wilhelm, Levent Akyuez, Armin Rehm, Uta E. Hoepken, Axel Pruss, Annette Kunkele, Ashley M. Jacobi, Hans-Dieter Volk, Michael Schmueck-Henneresse, Renata Stripecke, Petra Reinke, Dimitrios L. Wagner
Summary: This study optimized a virus-free genome-editing method to efficiently insert CAR into the TRAC locus of primary human T cells using CRISPR-Cas and double-stranded template DNA (dsDNA). Two pharmacological interventions were evaluated to improve cell viability and relative CAR knockin rates. The results showed that the combination of both interventions significantly increased CAR knockin rates and CAR T cell yield.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2022)
Article
Cell Biology
Gabriele Brachtl, Rodolphe Poupardin, Sarah Hochmann, Anna Raninger, Karsten Jurchott, Mathias Streitz, Stephan Schlickeiser, Michaela Oeller, Martin Wolf, Katharina Schallmoser, Hans-Dieter Volk, Sven Geissler, Dirk Strunk
Summary: Donor variation is an important issue limiting cell-based therapies. The use of human platelet lysate (hPL) instead of fetal bovine serum (FBS) to propagate bone marrow stromal cells (BMSCs) can have significant effects on BMSC phenotype and function. Notably, propagation in hPL increased BMSC proliferation and gene expression trajectories, resulting in distinct surface marker signatures after just one passage. The use of FBS as a culture medium led to declining proliferative potential and limited effects on DNA methylation. Culturing BMSCs in xenogenic serum resulted in loss of function during cartilage disk formation and increased clotting risk.
Article
Cell Biology
Bella Rossbach, Krithika Hariharan, Nancy Mah, Su-Jun Oh, Hans-Dieter Volk, Petra Reinke, Andreas Kurtz
Summary: This study investigates the immunological effects of hiPSC-derived cells and finds that their immune response varies depending on the stage of cell maturation. Autologous and allogeneic T cells tolerate early renal progenitors and mature renal epithelial cells, while NK cell responses are not suppressed and can effectively change NK cell activation status. These findings have important implications for the success of cell therapies.
Article
Cell Biology
Martin Wolf, Rodolphe W. Poupardin, Patricia Ebner-Peking, Andre Cronemberger Andrade, Constantin Bloechl, Astrid Obermayer, Fausto Gueths Gomes, Balazs Vari, Nicole Maeding, Essi Eminger, Heide-Marie Binder, Anna M. Raninger, Sarah Hochmann, Gabriele Brachtl, Andreas Spittler, Thomas Heuser, Racheli Ofir, Christian G. Huber, Zami Aberman, Katharina Schallmoser, Hans-Dieter Volk, Dirk Strunk
Summary: Nanoparticles derived from therapy-grade human placental-expanded stromal cells are surrounded by a protein corona, which improves skin regeneration and angiogenesis and induces differential signaling in immune cells.
JOURNAL OF EXTRACELLULAR VESICLES
(2022)
Article
Multidisciplinary Sciences
C. Kedor, H. Freitag, L. Meyer-Arndt, K. Wittke, L. G. Hanitsch, T. Zoller, F. Steinbeis, M. Haffke, G. Rudolf, B. Heidecker, T. Bobbert, J. Spranger, H. D. Volk, C. Skurk, F. Konietschke, F. Paul, U. Behrends, J. Bellmann-Strobl, C. Scheibenbogen
Summary: Some COVID-19 patients experience long-lasting symptoms, such as fatigue and exertion intolerance, even after recovery. These symptoms are similar to those seen in non-COVID-19 patients and may be related to low-level inflammation and hypoperfusion.
NATURE COMMUNICATIONS
(2022)
Article
Allergy
Robert Sabat, Deimante Simaite, Johann Eli Gudjonsson, Theresa-Charlotte Brembach, Katrin Witte, Torben Krause, Georgios Kokolakis, Eckart Bartnik, Christos Nikolaou, Natascha Rill, Bema Coulibaly, Clement Levin, Matthias Herrmann, Gabriela Salinas, Thomas Leeuw, Hans-Dieter Volk, Kamran Ghoreschi, Kerstin Wolk
Summary: This study aimed to characterize mediators that support B/plasma cell persistence in Hidradenitis suppurativa (HS), a chronic inflammatory disease. The results showed that levels of B/plasma cells, neutrophils, CD81 T cells, and M0 and M1 macrophages were elevated in HS lesions compared to healthy skin. It was found that BAFF, produced by myeloid cells, played a key role in supporting B/plasma cell persistence and function in HS lesions.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Christian H. Bucher, Julia C. Berkmann, Lisa-Marie Burkhardt, Carolin Paschke, Claudia Schlundt, Annemarie Lang, Angelique Wolter, Alexandra Damerau, Sven Geissler, Hans-Dieter Volk, Georg N. Duda, Katharina Schmidt-Bleek
Summary: Neutralizing immune protein could rejuvenate delayed bone fracture healing in elderly patients by reducing inflammatory responses. This study reveals the impact of immune profile on healing process and the role of interleukin-22 in delayed healing. Neutralizing IL-22 may offer a novel therapeutic strategy for accelerating healing in individuals with advanced immune experiences.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2022)
Article
Orthopedics
T. Winkler, M. L. Costa, R. Ofir, O. Parolini, S. Geissler, H-D Volk, C. Eder
Summary: The HIPGEN consortium aims to develop a cell therapy product for hip fracture patients using PLacental-eXpanded (PLX-PAD) stromal cells. The multicentre, multinational, randomized, double-blind, placebo-controlled trial involves 240 patients aged 60 to 90 years. The study evaluates the efficacy, safety, and tolerability of intramuscular PLX-PAD administration for the treatment of muscle injury following arthroplasty for hip fracture.
Article
Immunology
Florian Lammers-Lietz, Levent Akyuz, Insa Feinkohl, Cornelia Lachmann, Tobias Pischon, Hans-Dieter Volk, Clarissa von Haefen, Fatima Yurek, Georg Winterer, Claudia D. Spies
Summary: This study suggests that inflammation may contribute to the development of postoperative delirium. Elevated levels of IL-8 in plasma were observed in delirious patients, and whole blood IL-8 levels showed distinct patterns. Increased preoperative levels of IL-8 in whole blood were associated with delirium occurrence.
BRAIN, BEHAVIOR, & IMMUNITY - HEALTH
(2022)