Article
Cell Biology
Alessandro Fantin, Carlotta Tacconi, Emanuela Villa, Elena Ceccacci, Laura Denti, Christiana Ruhrberg
Summary: This study found that KIT is crucial for transient definitive hematopoiesis in the fetal liver during mouse embryo development, but dispensable for the process of yolk sac endothelial-to-hematopoietic transition.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell & Tissue Engineering
Yi Yu, Alejandra Vargas Valderrama, Zhongchao Han, Georges Uzan, Sina Naserian, Estelle Oberlin
Summary: The study demonstrates that fetal liver-MSCs have stronger immunosuppressive effects on T cells compared to adult bone marrow-MSCs, by inhibiting T cell proliferation and activation and inducing functional T regs.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Immunology
Rene Reitermaier, Thomas Krausgruber, Nikolaus Fortelny, Tanya Ayub, Pablo Augusto Vieyra-Garcia, Philip Kienzl, Peter Wolf, Anke Scharrer, Christian Fiala, Marita Kolz, Manuela Hiess, Martin Vierhapper, Christopher Schuster, Andreas Spittler, Christof Worda, Wolfgang Weninger, Christoph Bock, Rene Reitermaier, Adelheid Elbe-Buerger
Summary: Single-cell analyses identified a naive T cell population expressing specific T cell receptors enriched in fetal skin and intestine. These cells may contribute to early skin development and fetal immune defense, showing fundamental differences in immune surveillance between fetal and adult human skin.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Review
Hematology
Patricia Davenport, Zhi-Jian Liu, Martha Sola-Visner
Summary: Fetal and neonatal megakaryocyte progenitors are hyperproliferative and generate a large number of small, low-ploidy megakaryocytes. Recent studies have shown that these cells have all the characteristics of adult polyploid megakaryocytes, challenging the notion of immaturity. The unique features of fetal and neonatal megakaryopoiesis reflect a developmentally uncoupled process, allowing them to populate rapidly expanding bone marrow and blood volume.
Review
Cell & Tissue Engineering
Antonella Giancotti, Valentina D'Ambrosio, Sara Corno, Cristina Pajno, Guido Carpino, Gaia Amato, Flaminia Vena, Alessandro Mondo, Lorenzo Spiniello, Marco Monti, Ludovico Muzii, Daniela Bosco, Eugenio Gaudio, Domenico Alvaro, Vincenzo Cardinale
Summary: The fetal liver, with cells of both endodermal and mesenchymal origins, has the potential to be a source of regenerative medicine for liver and pancreatic diseases. However, the heterogeneity of cell isolation protocols and limited clinical studies hinder the widespread use of fetal liver cell therapy. To consolidate its role in regenerative medicine, further preclinical translational studies and large randomized controlled trials are needed, along with improved clinical and imaging assessments.
Article
Biochemistry & Molecular Biology
Hong Wu, Xuhao Zhou, Hui Gong, Zhichao Ni, Qingbo Xu
Summary: Perivascular stem/progenitor cells in the adipose layer and adventitia have the capability to differentiate into vascular cells, promoting intima hyperplasia or endothelial regeneration, and possess therapeutic potentials in vascular diseases. These cells play pivotal roles in vascular development and disease progression.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Immunology
Grace Nauman, Nichole M. Danzl, Jaeyop Lee, Chiara Borsotti, Rachel Madley, Jianing Fu, Markus A. Holzl, Alexander Dahmani, Akaitz Dorronsoro Gonzalez, Estefania Chavez, Sean R. Campbell, Suxiao Yang, Prakash Satwani, Kang Liu, Megan Sykes
Summary: PI mice transplanted with human fetal thymus and adult bone marrow CD34+ cells show reduced human reconstitution levels compared to Hu/Hu mice. The reduced numbers of APC progenitors in PI mice reflect the differences in the source of CD34+ cells used for transplantation. Overall, improvements in PI mouse models allow for a longer time frame for studying immune disease pathogenesis and immunotherapies.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Cell Biology
Marta Perez-Frances, Maria Valentina Abate, Delphine Baronnier, Philipp E. Scherer, Yoshio Fujitani, Fabrizio Thorel, Pedro L. Herrera
Summary: This study investigates the developmental dynamics of pancreatic islet endocrine cell types using murine models. The researchers find that adult islet cells originate from embryonic hormone-expressing cells and there is no evidence of islet cell differentiation from precursor cells after birth. The study also reveals specific patterns of hormone gene activation and switching during islet morphogenesis.
Article
Cell & Tissue Engineering
Thomas J. Streef, Esmee J. Groeneveld, Tessa van Herwaarden, Jesper Hjortnaes, Marie Jose Goumans, Anke M. Smits
Summary: This study identified the composition and regulators of developmental processes in human fetal epicardium using single-cell RNA sequencing, providing a valuable platform for studying the developing epicardium in detail.
Article
Oncology
Khaled Zohni, Lianet Lopez, Poonam Mander, Peter Szaraz, Melissa Filice, Brandon A. Wyse, Meredith Garcia, Itai Gat, Karen Glass, Andree Gauthier-Fisher, Clifford L. Librach
Summary: Chemotherapies can lead to germ cell depletion and gonadal failure. In this study, first trimester human umbilical cord perivascular cells (HUCPVCs) were shown to resist the cytotoxic effects of cyclophosphamide and prevent ovarian damage in rodent models of chemotherapy-induced gonadal injury. These cells demonstrate promising potential for fertility preservation.
Article
Cell & Tissue Engineering
Yoon Jung Choi, Adam M. Heck, Brian J. Hayes, Daniel Lih, Samuel G. Rayner, Brandon Hadland, Ying Zheng
Summary: The human fetal liver endothelium plays a unique role in supporting the maturation and expansion of multilineage hematopoietic stem and progenitor cells, with EC-derived WNT5A identified as a key factor in this process. The study highlights the significance of organ-specific endothelial niche in hematopoietic development and provides insights into potential signals for HSPC expansion in clinical applications.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Neurosciences
Muhammad N. Arshad, Simon Oppenheimer, Jaye Jeong, Bilge Buyukdemirtas, Janice R. Naegele
Summary: GABAergic interneurons play a crucial role in regulating adult neurogenesis and their transplantation can alleviate seizures and increase synaptic inhibition in temporal lobe epilepsy. The transplantation also affects the types of progenitor cells involved in adult neurogenesis.
NEUROBIOLOGY OF DISEASE
(2022)
Article
Multidisciplinary Sciences
Rashmi Bhardwaj, Lalit Kumar, Deepika Chhabra, N. K. Mehra, Atul Sharma, Sujata Mohanty, Vinod Kochupillai
Summary: This study explores the expansion of fetal liver hematopoietic stem cells in vitro, finding that a specific combination of 5 cytokines can optimally increase cell numbers with a cell viability greater than 90% throughout the 21-day culture period.
SCIENTIFIC REPORTS
(2021)
Article
Genetics & Heredity
Yaoxian Xu, Christoph Kuppe, Javier Perales-Paton, Sikander Hayat, Jennifer Kranz, Ali T. Abdallah, James Nagai, Zhijian Li, Fabian Peisker, Turgay Saritas, Maurice Halder, Sylvia Menzel, Konrad Hoeft, Annegien Kenter, Hyojin Kim, Claudia R. C. van Roeyen, Michael Lehrke, Julia Moellmann, Thimoteus Speer, Eva M. Buhl, Remco Hoogenboezem, Peter Boor, Jitske Jansen, Cordula Knopp, Ingo Kurth, Bart Smeets, Eric Bindels, Marlies E. J. Reinders, Carla Baan, Joost Gribnau, Ewout J. Hoorn, Joachim Steffens, Tobias B. Huber, Ivan Costa, Jurgen Floege, Rebekka K. Schneider, Julio Saez-Rodriguez, Benjamin S. Freedman, Rafael Kramann
Summary: Adult kidney organoids, known as tubuloids, are derived from a distinct CD24(+) epithelial subpopulation and can be used to model autosomal dominant polycystic kidney disease (ADPKD) and study drug response. The study found similarities in gene expression between gene-edited tubuloids and ADPKD patients' tissue, demonstrating the potential of tubuloids in ADPKD disease modeling. Additionally, the approved drug for ADPKD, tolvaptan, was found to have a significant effect on cyst size in tubuloids but not in pluripotent stem cell-derived models.
Article
Cell Biology
Suwei Gao, Qiang Shi, Yifan Zhang, Guixian Liang, Zhixin Kang, Baofeng Huang, Dongyuan Ma, Lu Wang, Jianwei Jiao, Xiangdong Fang, Cheng-Ran Xu, Longqi Liu, Xun Xu, Berthold Gottgens, Cheng Li, Feng Liu
Summary: This study identified novel transcriptionally heterogeneous subsets of HSC/MPP in mouse fetal liver, including a CD93-enriched subset with enhanced stem cell properties, as well as 'pocket-like' units called HSC PLUS enriched with growth factors and niche cells. Unexpectedly, macrophages were found to play a supportive role in HSC/MPP expansion, along with growth factors such as MDK, PTN, and IGFBP5. Furthermore, while conserved cell-cell interactions and expansion mechanisms were observed between mouse and human FL HSCs/MPPs, their transcriptome signatures showed divergence.
Review
Gastroenterology & Hepatology
Laurent Dolle, Neil D. Theise, Eva Schmelzer, Luke Boulter, Olivier Gires, Leo A. van Grunsven
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2015)
Article
Biotechnology & Applied Microbiology
Eva Schmelzer, Anthony Finoli, Ian Nettleship, Joerg C. Gerlach
BIOTECHNOLOGY AND BIOENGINEERING
(2015)
Article
Cell & Tissue Engineering
Giada Pietrosi, Giovanni Vizzini, Jorg Gerlach, Cinzia Chinnici, Angelo Luca, Giandomenico Amico, Monica D'Amato, Pier Giulio Conaldi, Sergio Li Petri, Marco Spada, Fabio Tuzzolino, Luigi Alio, Eva Schmelzer, Bruno Gridelli
CELL TRANSPLANTATION
(2015)
Article
Cell Biology
Roger Esteban-Vives, Matt Young, Patrick Over, Eva Schmelzer, Alain Corcos, Jenny Ziembicki, Joerg Gerlach
Article
Cell & Tissue Engineering
William C. W. Chen, James E. Baily, Mirko Corselli, Mary E. Diaz, Bin Sun, Guosheng Xiang, Gillian A. Gray, Johnny Huard, Bruno Peault
Article
Cell & Tissue Engineering
Christopher Pekor, Joerg C. Gerlach, Ian Nettleship, Eva Schmelzer
TISSUE ENGINEERING PART C-METHODS
(2015)
Review
Medicine, Research & Experimental
Huidong Zhou, Hong Liu, Mohamed Ezzelarab, Eva Schmelzer, Yi Wang, Joerg Gerlach, Bruno Gridelli, David K. C. Cooper
XENOTRANSPLANTATION
(2015)
Article
Engineering, Biomedical
Eva Schmelzer, Patrick Over, Bruno Gridelli, Jorg C. Gerlach
JOURNAL OF MEDICAL AND BIOLOGICAL ENGINEERING
(2016)
Article
Multidisciplinary Sciences
M. J. Pont, M. W. Honders, A. N. Kremer, C. van Kooten, C. Out, P. S. Hiemstra, H. C. de Boer, M. J. Jager, E. Schmelzer, R. G. Vries, A. S. Al Hinai, W. G. Kroes, R. Monajemi, J. J. Goeman, S. Bohringer, W. A. F. Marijt, J. H. F. Falkenburg, M. Griffioen
Review
Cell & Tissue Engineering
Arman Saparov, Vyacheslav Ogay, Talgat Nurgozhin, Medet Jumabay, William C. W. Chen
STEM CELLS INTERNATIONAL
(2016)
Article
Materials Science, Biomaterials
Qinghao Zhang, Eva Schmelzer, Joerg C. Gerlach, Ian Nettleship
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2017)
Article
Medicine, Research & Experimental
Hayato Iwase, Hong Liu, Eva Schmelzer, Mohamed Ezzelarab, Martin Wijkstrom, Hidetaka Hara, Whayoung Lee, Jagjit Singh, Cassandra Long, Eric Lagasse, Joerg C. Gerlach, David K. C. Cooper, Bruno Gridelli
XENOTRANSPLANTATION
(2017)
Article
Biochemistry & Molecular Biology
Xueqin Gao, Arvydas Usas, Jonathan D. Proto, Aiping Lu, James H. Cummins, Alexander Proctor, Chien-Wen Chen, Johnny Huard
Article
Gastroenterology & Hepatology
Joerg C. Gerlach, Patrick Over, Hubert G. Foka, Morris E. Turner, Robert L. Thompson, Bruno Gridelli, Eva Schmelzer
HEPATOLOGY RESEARCH
(2015)
Article
Multidisciplinary Sciences
William C. W. Chen, Leonid Gaidukov, Yong Lai, Ming-Ru Wu, Jicong Cao, Michael J. Gutbrod, Gigi C. G. Choi, Rachel P. Utomo, Ying-Chou Chen, Liliana Wroblewska, Manolis Kellis, Lin Zhang, Ron Weiss, Timothy K. Lu
Summary: In this study, a highly tunable, modular, and versatile CRISPR-based synthetic transcription system was developed for programmable control of gene expression and cellular phenotypes in mammalian cells. The system allows for predictable and tunable regulation of gene expression and cellular function in multiple cell types.
NATURE COMMUNICATIONS
(2022)