Review
Cell Biology
Vadim I. Chechekhin, Konstantin Yu. Kulebyakin, Romesh I. Kokaev, Pyotr A. Tyurin-Kuzmin
Summary: Adipose tissue relies on the regulation of multipotent mesenchymal stromal cells (MSCs), which serve as both precursors for new adipocytes and key paracrine regulators of adipose tissue homeostasis. The activity of MSCs is tightly controlled, regulating aspects such as proliferation, differentiation, and signal molecule production through modulation of hormonal sensitivity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell & Tissue Engineering
Mauro Krampera, Katarina Le Blanc
Summary: MSCs have shown exceptional safety in clinical trials, but reliable potency assays to predict their immunosuppressive efficacy are still lacking. They are approved for certain diseases in Japan and Europe, but not in the United States. Researchers discuss potential mechanisms of action, experimental models, and approaches for identifying MSC effects in vivo.
Review
Cell & Tissue Engineering
Hesham Soliman, Marine Theret, Wilder Scott, Lesley Hill, Tully Michael Underhill, Boris Hinz, Fabio M. Rossi
Summary: MSCs are essential for the development, maintenance, function, and regeneration of most tissues. They play a crucial role in responding to injury, promoting regeneration through secretion of trophic factors and ECM molecules, and contributing to fibrotic reparative processes. Through fate mapping and single cell omics, tissue-specific MSC identities and functions are being elucidated, providing unprecedented insights into the secret lives of tissue-resident MSCs.
Article
Neurosciences
Vitalii Kyryk, Oleg Tsupykov, Alina Ustymenko, Ekaterina Smozhanik, Iryna Govbakh, Gennadii Butenko, Galyna Skibo
Summary: The study aimed to investigate the ultrastructural characteristics of 3D spheroids formed by adipose-derived multipotent mesenchymal stromal cells (ADSCs) of ovariectomized mice of different ages. The findings suggest that aging has a negative impact on the ultrastructural characteristics of 3D spheroids formed by ADSCs in old mice compared to young mice.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Cell & Tissue Engineering
Gertraud Eylert, Reinhard Dolp, Alexandra Parousis, Richard Cheng, Christopher Auger, Magdalena Holter, Ingrid Lang-Olip, Viola Reiner, Lars-Peter Kamolz, Marc G. Jeschke
Summary: This study suggests that for full-thickness burn excised wound regeneration, the optimal cell dosage range is the low dose range of 200 to 40,000 cells/cm(2), rather than the traditional belief that more cells lead to better healing outcomes.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell & Tissue Engineering
Rose Triantafillia Psaroudis, Urvashi Singh, Maximilien Lora, Peter Jeon, Abigail Boursiquot, Ursula Stochaj, David Langlais, Ines Colmegna
Summary: This study identifies DPP4 gene expression and CD26 protein abundance as markers of replicative senescence in MSC(AT). MSCs with high CD26 expression have reduced immunopotency, and senolytic treatment can decrease the frequency of CD26(high) MSCs.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell Biology
Mariachiara Zuccarini, Catia Lambertucci, Marzia Carluccio, Patricia Giuliani, Maurizio Ronci, Andrea Spinaci, Rosaria Volpini, Renata Ciccarelli, Patrizia Di Iorio
Summary: The study found that A(1)R partial agonists or antagonists can limit the differentiation of adipose tissue cells into adipocytes, thereby preventing an increase in fat mass, which may help manage metabolic syndrome in obese individuals.
Article
Cell Biology
Nataliya Petinati, Irina Shipounova, Natalia Sats, Alena Dorofeeva, Alexandra Sadovskaya, Nikolay Kapranov, Yulia Tkachuk, Anatoliy Bondarenko, Margarita Muravskaya, Michail Kotsky, Irina Kaplanskaya, Tamara Vasilieva, Nina Drize
Summary: This article investigates the properties of BM-MSCs before and after implantation under the renal capsule in a mini pig model. It is found that the implants formed ectopic foci containing bones, ectopic hematopoiesis, bone marrow stromal cells, and muscle cells. The cells that migrated out from these implants meet the criteria for MSCs and are designated as IM-MSCs.
Article
Cell & Tissue Engineering
Fabiana Zanata, Lowry Curley, Elizabeth Martin, Annie Bowles, Bruce A. Bunnell, Xiying Wu, Lydia Masako Ferreira, Jeffrey M. Gimble
Summary: This study found similarities between ASC and DF in terms of surface marker expression, differentiation potential, and gene expression, but ASC may have a relatively stronger adipogenesis. However, there is substantial overlap between ASC and DF phenotypes based on biochemical and differentiation metrics.
STEM CELLS AND DEVELOPMENT
(2021)
Article
Biochemistry & Molecular Biology
Gabriela Gorczyca, Kamil Wartalski, Jerzy Wiater, Marcin Samiec, Zbigniew Tabarowski, Malgorzata Duda
Summary: Ndn and Bdn, synthetic testosterone analogues, at pharmacological doses, can increase cancer stem cell markers and negatively impact growth of potential stem cells, potentially enhancing risk of neoplastic transformation. Detailed molecular characterization of these cells and their derivatives may aid in further investigations on nuclear donor cells and cloning by somatic cell nuclear transfer, providing insights into genetic and epigenetic mechanisms in tumorigenesis and potential reversal.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Mike Wagenbrenner, Konrad Poker, Tizian Heinz, Marietta Herrmann, Konstantin Horas, Regina Ebert, Susanne Mayer-Wagner, Boris M. Holzapfel, Maximilian Rudert, Andre F. Steinert, Manuel Weissenberger
Summary: This study compared the in vitro chondrogenic potential of MSC-like cells from different tissue sources and found that regardless of the cell source, all MSC-like cells showed multipotent differentiation potential. This provides insight into the suitability of various tissue origins for cell-based cartilage therapies and tissue engineering, leading to increased flexibility in cell availability.
APPLIED SCIENCES-BASEL
(2022)
Article
Medicine, Research & Experimental
Elisa Montanari, Luca Szabo, Alexandre Balaphas, Jeremy Meyer, Nadja Perriraz-Mayer, Joel Pimenta, Marie-Noelle Giraud, Bernhard Egger, Sandrine Gerber-Lemaire, Leo Buhler, Carmen Gonelle-Gispert
Summary: Neonatal and juvenile porcine islet cell clusters (ICC) are considered as an unlimited source for islet xenotransplantation to treat type 1 diabetes patients. Multipotent mesenchymal stromal cells derived from the same pancreata (pMSC) were found to improve insulin secretion of ICC through direct cell-cell contact, indicating a potential role in supporting beta-cell function in transplantation. Further development of new anti-fibrotic polymers or genetically modified pigs with lower immunogenicity may be necessary for successful clinical application.
XENOTRANSPLANTATION
(2021)
Article
Cell Biology
Janina Burk, Michaela Melzer, Alina Hagen, Katrin Susanne Lips, Katja Trinkaus, Ariane Nimptsch, Jenny Leopold
Summary: This study investigated the applicability of lipid phenotyping for MSC characterization and discrimination, finding differences in phospholipid species diversity between MSC from different species, and identified potentially suitable lipid markers across culture conditions. Phospholipid profiling could be a promising approach for MSC characterization, allowing for some heterogeneity within MSC while still achieving clear discrimination from fibroblasts.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Wen-Xiang Liu, Chun-Xiao Li, Xin-Xiang Xie, Wei Ge, Tian Qiao, Xiao-Feng Sun, Wei Shen, Shun-Feng Cheng
Summary: According to estimations, around 15% of couples worldwide suffer from infertility, especially individuals with azoospermia or oocyte abnormalities. Recent breakthroughs in stem cell intervention for infertility treatment include the differentiation of skin-derived stem cells (SDSCs) into primordial germ cell-like cells (PGCLCs). However, the cellular origin of SDSCs and their transcriptional changes during differentiation into PGCLCs in vitro are still not well understood. This study provides novel insights by revealing that porcine SDSCs are derived from multipotent dermal fibroblast progenitors (MDFPs), and they can effectively differentiate into PGCLCs through complex transcriptional regulation and histone modification.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Cell Biology
Kamil Wartalski, Gabriela Gorczyca, Jerzy Wiater, Zbigniew Tabarowski, Malgorzata Duda
Summary: The study demonstrated that porcine ovarian putative stem cells (poPSCs) have the potential to differentiate into endothelial cells (ECs) and develop capillary networks under appropriate induction, indicating their suitability as a potential source of ECs for tissue engineering.
HISTOCHEMISTRY AND CELL BIOLOGY
(2021)